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All Journal Buletin Peternakan Jurnal Ilmu Ternak Veteriner AGRISAINTIFIKA Jurnal Ilmu-ilmu Pertanian AGRITROPICA : Journal of Agricultural Sciences Jurnal Hilirisasi IPTEKS (JHI) Wahana Peternakan Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Agripet
Kusnadidi Subekti
Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Engineering Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Identification of Myostatin Gene Polymorphism (MSTN|Taq1) Exon-2 in Pitalah Duck Using The PCR-RFLP Method Subekti, Kusnadidi; Salsabila, Winanti; Samara, Nada Iswari; Arlina, Firda
Buletin Peternakan Vol 49, No 3 (2025): BULETIN PETERNAKAN VOL. 49 (3) August 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i3.106398

Abstract

This study aimed to identify the polymorphism of the exon-2 myostatin gene (MSTN) in Pitalah ducks using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method. 28 blood samples of Pitalah ducks were used in this study. Blood samples were extracted using Intron Biotechnology's Intron G-SpinTM Total DNA Extraction Kit protocol. The DNA extraction results were then amplified using a pair of primers, namely the Forward: 5' TCCACTTGTTACTGATGCTGT-3' primer and the Reverse: 5'- TAGGGAAATGGAGGCACAGG-3' primer, with a fragment target of 700 bp. Furthermore, the amplified product is restricted using the Taq1 enzyme, which recognizes the cutting site on T|CGA. Based on the results of the study, two genotypes were found, namely 89.29% truncated homozygous (+/+) and heterozygous (+/-), as well as 10.71% of the total sample used, while untruncated homozygous genotypes (-/-) were not found in this study. Meanwhile, the allele frequency (+) was 0.946, the allele frequency (-) was 0.054, and the observed heterozygosity value was greater than the expected heterozygosity value (Ho>He). This study concludes that the myostatin exon-2 gene in Pitalah ducks is polymorphic and is in Hardy-Weinberg equilibrium.
ANALISIS KERAGAMAN GEN PROLAKTIN (PRL) PADA ITIK PITALAH; ITIK LOKAL SUMATERA BARAT Amelia, Riska; Rusfidra, Rusfidra; Subekti, Kusnadidi; Arlina, Firda; Husmaini, Husmaini; Haryati, Stefani Fitri
Agrisaintifika: Jurnal Ilmu-Ilmu Pertanian Vol. 7 No. 2(is) (2023): Pengembangan Pertanian Berbasis Kearifan Lokal Yang Berkelanjutan
Publisher : Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/ags.v7i2(is).4359

Abstract

AbstrakProlaktin merupakan hormon polipeptida yang disekresikan oleh kelenjar pituitary anterior dan secara spesifik kandidat gen yang mengontrol variasi jumlah produksi telur dengan reduksi biosintesis telur dalam beberapa periode mengeram. Tujuan penelitian ini  adalah untuk menganalisis keragaman gen Prolaktin pada itik Pitalah. Ekstraksi yang dilakukan pada sampel darah 45 ekor itik Pitalah dengan menggunakan protocol G-spinTM Total DNA Extraction Mini Kit dari Intron Biotechnology. Selanjutnya diamplifikasi dengan menggunakan sepasang primer forward 5’- CTG CAT CTG TGG ACA TTG CT-3’ dan reverse 5’GAA GCA GGT TTG GGA GTA CG-3’ dengan target fragmen 496 bp dan disekuensing menggunakan jasa 1sr Base Singapore. Data hasil sekuensing dianalisis menggunakan Seqmen DNASTAR dan MEGA 11 dan menunjukkan 14 keragaman pada daerah ekson 4 dan parsial intron 3 dan 4 pada titik mutasi INDEL 3703A, INDEL3939A, C3974A, A4030G, A4038C, T4056C, G4061A, T4091G, T4094A, T4098C, G4102A, C4106A, A4107C dan C4154G. Keragaman gen Prolaktin bersifat polimorfik dengan frekuensi genotype tidak dalam keseimbangan Hardy-Weinberg. Kata Kunci: Prolaktin, Itik Pitalah, Keragaman, Mutasi, Mutasi, Sekuensing
Co-Authors Amri Syahardi Ananda Ananda Arfan Hanafi Arif Darmawan Asep Gunawan Cece Sumanri Cici Wulandari, Cici Dedi Duryadi Solihin Firda Arlina Firdaus, Stevanny Husmaini Husmaini Husmaini, Husmaini Idrasit Paldi Jafrinur James Hellyward Khalil Khalil Riska Amelia Robi Amizar Rudi Afnan Rusfidra rusfidra Rusli, Ridho Kurniawan Sabrina Sabrina Sabrina Sabrina Sabrina Sabrina Salsabila, Winanti Samara, Nada Iswari SATRIYAS ILYAS Sosmiarti Sosmiarti Stefani Fitri Haryati Ulvira Rifni Wizna Wizna WULANDARI, MERI Zurmiati