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All Journal Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Narra J eJournal Kedokteran Indonesia Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal) Jurnal Kesehatan
Andon Hestiantoro
Department Of Obstetrics And Gynecology, Faculty Of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Exploring the Potential of Lifestyle and Dietary Intervention to Improve In Vitro Fertilization Outcomes in Overweight and Obese Women: Systematic Review and Meta-Analysis Abdullah, Muhammad Raoul Taufiq; Hestiantoro, Andon
Jurnal Ilmu Kesehatan Masyarakat Vol. 14 No. 06 (2025): Jurnal Ilmu Kesehatan Masyarakat
Publisher : UIMA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33221/jikm.v14i06.4346

Abstract

Lifestyle and dietary interventions have been proposed to improve in vitro fertilization (IVF) outcomes in women with overweight and obesity, conditions known to reduce reproductive success. This systematic review followed PRISMA guidelines and searched PubMed, MEDLINE, and Scopus up to July 2025. Four randomized controlled trials involving 433 participants met inclusion criteria. A fixed-effects meta-analysis showed that weight loss interventions significantly increased clinical pregnancy rates (OR 1.55; 95% CI 1.03–2.35; p=0.04), with low heterogeneity (I²=32%). The strongest effect was reported in Sim 2014 (OR 5.07; 95% CI 1.21–21.28). Improvement in IVF outcomes is likely mediated through enhanced insulin sensitivity, reduced inflammation, and better hormonal regulation. Although both obesity and advanced maternal age reduce fertility, obesity has a greater impact on IVF success in younger women; therefore, weight reduction may be particularly beneficial for women under 35–38 years. In contrast, delaying treatment for weight loss may disadvantage older women due to age-related fertility decline. Overall, lifestyle-based weight loss significantly improves IVF outcomes and should be integrated into treatment planning for overweight and obese patients.
Comparison of DNA Extraction Feasibility from Menstrual Blood and Endometrial Tissue in Reproductive-Aged Women Febriyeni; Hestiantoro, Andon; Asmarinah; Tulandi, Togas; Hasnita, Evi; Zuraida; Harnaldo Putra, Billy; Octariyana; Rizki Amanda, Clara; Astria Rishel, RIka
Jurnal Kesehatan - STIKes Prima Nusantara Vol 16 No 2 (2025): Jurnal Kesehatan Volume 16 Nomor 2 Tahun 2025
Publisher : LPPM Universitas Prima Nusantara Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35730/jk.v16i2.1314

Abstract

ABSTRACT The endometrium is a highly vascularized tissue composed of numerous blood vessels, with cells that are formed and destroyed during each menstrual cycle. Menstrual blood, which is shed cyclically from the endometrium in women of reproductive age, contains endometrial tissue or cells. Both deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are the primary nucleic acids present in cells with nuclei. In this study, 20 samples of menstrual blood and endometrial tissue were collected from women of reproductive age. Menstrual blood was gathered using specially designed filter paper, and DNA was extracted using the QIAamp DNA Mini Kit (Qiagen, Cat No.: 51304). Endometrial tissue was obtained via biopsy, and DNA was extracted using the Geneaid DNA Isolation Kit (Geneaid, New Taipei, Taiwan). DNA purity and concentration were measured using a Thermo Scientific Nano-Drop microvolume Spectrophotometer. The results indicated an average DNA purity of 1.88 ± 0.09 and a mean concentration of 116.9 ng/µL for menstrual blood, while endometrial tissue had a median DNA purity of 1.92 and a concentration of 192 ng/µL. Statistical analysis revealed no significant difference in DNA purity between menstrual blood and endometrial tissue (p = 0.083), but a significant difference in DNA concentration was observed (p = 0.002). Although there was a slight difference in DNA concentration, both menstrual blood and endometrial tissue samples were equally effective in preserving optimal purity and concentration of DNA molecules. Keywords: Deoxyribonucleic Acid, DNA, Endometrial Tissue, Menstrual Blood, Nucleic Acid.
Co-Authors A. Abadi Abdullah, Muhammad Raoul Taufiq Achmad K. Harzif Achmad K. Harzif Achmad Kemal Achmad Kemal Harzif Aldri Frinaldi Ali Baziad Andrew Kurniawan Ario Legiantuko Asmarinah Asmarinah Asmarinah Astria Rishel, RIka Astuti, Brilliant Putri Kusuma Bagastoro, Aswan Baidah, Priska A Betaria Putri Billy Harnaldo Putra, Dini Fatmi, Bram Pradipta Budi Wiweko Caroline G. Puspita Christopher Yo, Edward Danang T. Pamungkas Dedi Afandi Dick F. Swaab Eka R. Gunardi Elisia Atnil Eliza Mansyur Evi Hasnita Fadilah Fadilah, Fadilah Fakhri, Aldithya Faradina, Anindita Abigail Febriyeni Febriyeni, Febriyeni Ferry Sandra Gita Pratama Hari Kurniawan, Riyan Hartanto Bayuaji Herbert Situmorang Herbert Situmorang Indah S. Widyahening Intan Kusumaningtyas Intan Kusumaningtyas Irzanti, Alisha Nurdya Iswara, Raja Al Fath Widya Jasirwan, Shanty Olivia Kanadi Sumapradja Kemal, Achmad Legiantuko, Ario Martina Wiwie Setiawan Nasrun Maryam Ulfa Mila Maidarti Muharam, Muharam Muharam, R Naylah Muna Nurdya Irzanti, Alisha Nurhadi Ibrahim Octariyana Oki Riayati Ponco Birowo Prasetya, David Eka Priska A Baidah Puspita E. Wuyung Raden Muharam Natadisastra Raymond R. Tjandrawinata Raymond Surya Retno A. Werdhani Ririn Rahmala Febri Rizki Amanda, Clara Robert H. Purwaka, Robert H. Saraswati, Jaya Shadrina, Amalia Silvana, Vita Suryoadji, Kemal Akbar Tiara Aninditha Tita Yuningsih Trinovita Andraini Tulandi, Togas Vita Silvana Yo, Edward Christopher Yudianto B Saroyo Yuli Budiningsih Zuraida