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All Journal Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Narra J eJournal Kedokteran Indonesia Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal) Jurnal Kesehatan
Andon Hestiantoro
Department Of Obstetrics And Gynecology, Faculty Of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Comparison of DNA Extraction Feasibility from Menstrual Blood and Endometrial Tissue in Reproductive-Aged Women Febriyeni; Hestiantoro, Andon; Asmarinah; Tulandi, Togas; Hasnita, Evi; Zuraida; Harnaldo Putra, Billy; Octariyana; Rizki Amanda, Clara; Astria Rishel, RIka
Jurnal Kesehatan - STIKes Prima Nusantara Vol 16 No 2 (2025): Jurnal Kesehatan Volume 16 Nomor 2 Tahun 2025
Publisher : LPPM Universitas Prima Nusantara Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35730/jk.v16i2.1314

Abstract

ABSTRACT The endometrium is a highly vascularized tissue composed of numerous blood vessels, with cells that are formed and destroyed during each menstrual cycle. Menstrual blood, which is shed cyclically from the endometrium in women of reproductive age, contains endometrial tissue or cells. Both deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are the primary nucleic acids present in cells with nuclei. In this study, 20 samples of menstrual blood and endometrial tissue were collected from women of reproductive age. Menstrual blood was gathered using specially designed filter paper, and DNA was extracted using the QIAamp DNA Mini Kit (Qiagen, Cat No.: 51304). Endometrial tissue was obtained via biopsy, and DNA was extracted using the Geneaid DNA Isolation Kit (Geneaid, New Taipei, Taiwan). DNA purity and concentration were measured using a Thermo Scientific Nano-Drop microvolume Spectrophotometer. The results indicated an average DNA purity of 1.88 ± 0.09 and a mean concentration of 116.9 ng/µL for menstrual blood, while endometrial tissue had a median DNA purity of 1.92 and a concentration of 192 ng/µL. Statistical analysis revealed no significant difference in DNA purity between menstrual blood and endometrial tissue (p = 0.083), but a significant difference in DNA concentration was observed (p = 0.002). Although there was a slight difference in DNA concentration, both menstrual blood and endometrial tissue samples were equally effective in preserving optimal purity and concentration of DNA molecules. Keywords: Deoxyribonucleic Acid, DNA, Endometrial Tissue, Menstrual Blood, Nucleic Acid.
Clinical Pregnancy Rate in in-Vitro Fertilization (IVF) among Endometrioma Patients Underwent Cystectomy Laparoscopy : Kejadian Kehamilan Klinis setelah Fertilisasi In Vitro pada Pasien Endometrioma Postsistektomi Laparoskopi Kurniawan, Andrew; Ulfa, Maryam; Surya, Raymond; Hestiantoro, Andon
Indonesian Journal of Obstetrics and Gynecology Volume 10 No. 3 July 2022
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32771/inajog.v10i3.1566

Abstract

Abstract Objective: to discuss the clinical pregnancy rate and factors influencing fecundity among endometriosis women having cystectomy laparoscopically who underwent IVF. Methods: The search was conducted on Pubmed®, EBSCOhost®, and Proquest®, Cochrane Library®, ClinicalKey® using MeSH. Case : A 38-years woman, P0A0 come to the gynecology clinic with a chief complaint of primary infertility for eight years. The patient had undergone a bilateral endometrial cyst resected with laparoscopy and failed for two cycles of IVF. What is the clinical pregnancy rate of women that undergo IVF with a history of ovarian endometrioma cystectomy? Results:, There were six articles appropriate to the inclusion criteria and further appraised using the Centre for Evidence-Based Medicine, University of Oxford appraisal tools. The studies consisted of five prognostic studies and one meta-analysis. The studies were appraised for their validity, Importance, and Applicability. Conclusion: The pregnancy rate in patients undergoing IVF after ovarian cystectomy ranges from 14%-45.2%. A prudent consideration and carefulness during cystectomy surgery are essential for patients that wish to be fertile. Keywords: clinical pregnancy rate, endometrioma, in vitro fertilization, laparoscopy, ovarian cystectomy Abstrak Tujuan: Untuk menelaah tentang kemungkinan terjadinya kehamilan secara klinis pada perempuan yang menjalani IVF dengan riwayat laparoskopi sistektomi dan faktor-faktor yang mempengaruhi kesuburannya. Metode: Pencarian jurnal dilakukan dengan menggunakan search engine Pubmed®, EBSCOhost®, dan Proquest®, Cochrane Library®, ClinicalKey® menggunakan MeSH. Kasus: Perempuan P0A0 berusia 38 tahun datang ke poliklinik dengan keluhan utama infertilitas selama delapan tahun. Pasien memiliki riwayat kista endometrioma bilateral yang sudah di kistektomi dengan laparoskopi dan dua kali gagal menjalani siklus IVF. Berapa kemungkinan terjadinya kehamilan secara klinis pada perempuan yang menjalani IVF dengan riwayat kistektomi endometrioma? Hasil: Dari hasil pencarian didapatkan enam artikel jurnal yang sesuai dengan kriteria inklusi dan dianalisis lebih lanjut menggunakan telaah kritis Evideence-Based Medicine, University of Oxford. .Terdapat lima jurnal studi prognostic dan satu meta-analisis. Studi-studi tersebut akan ditelaah kritis lebih lanjut berdasarkan validitas, kepentingan, dan aplikabilitasnya Kesimpulan: Kemungkinan terjadinya kehamilan secara klinis pada pasien yang menjalani IVF setelah kistektomi endometrioma ovarium adalah 14%-45,2%. Pertimbangan yang bijaksana dan hati-hati diperlukan untuk melakukan operasi kistektomi pada pasien yang mengalami endometrioma dan ingin tetap subur. Kata kunci: endometrioma, fertilisasi in vitro, kehamilan klinis, kistektomi ovarium, laparoskopi
Co-Authors A. Abadi Abdullah, Muhammad Raoul Taufiq Achmad K. Harzif Achmad K. Harzif Achmad Kemal Achmad Kemal Harzif Aldri Frinaldi Ali Baziad Ario Legiantuko Asmarinah Asmarinah Asmarinah Astria Rishel, RIka Astuti, Brilliant Putri Kusuma Bagastoro, Aswan Baidah, Priska A Betaria Putri Billy Harnaldo Putra, Dini Fatmi, Bram Pradipta Budi Wiweko Caroline G. Puspita Christopher Yo, Edward Danang T. Pamungkas Dedi Afandi Dick F. Swaab Eka R. Gunardi Elisia Atnil Eliza Mansyur Evi Hasnita Fadilah Fadilah, Fadilah Fakhri, Aldithya Faradina, Anindita Abigail Febriyeni Febriyeni, Febriyeni Ferry Sandra Gita Pratama Hari Kurniawan, Riyan Hartanto Bayuaji Herbert Situmorang Herbert Situmorang Indah S. Widyahening Intan Kusumaningtyas Intan Kusumaningtyas Irzanti, Alisha Nurdya Iswara, Raja Al Fath Widya Jasirwan, Shanty Olivia Kanadi Sumapradja Kemal, Achmad Kurniawan, Andrew Legiantuko, Ario Martina Wiwie Setiawan Nasrun Mila Maidarti Muharam, Muharam Muharam, R Naylah Muna Nurdya Irzanti, Alisha Nurhadi Ibrahim Octariyana Oki Riayati Ponco Birowo Prasetya, David Eka Priska A Baidah Puspita E. Wuyung Raden Muharam Natadisastra Raymond R. Tjandrawinata Raymond Surya Retno A. Werdhani Ririn Rahmala Febri Rizki Amanda, Clara Robert H. Purwaka, Robert H. Saraswati, Jaya Shadrina, Amalia Silvana, Vita Suryoadji, Kemal Akbar Tiara Aninditha Tita Yuningsih Trinovita Andraini Tulandi, Togas Ulfa, Maryam Vita Silvana Yo, Edward Christopher Yudianto B Saroyo Yuli Budiningsih Zuraida