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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) Majalah Ilmiah Peternakan BIOTROPIA - The Southeast Asian Journal of Tropical Biology Suluh Pembangunan: Journal of Extension and Development BERITA BIOLOGI ANNALES BOGORIENSES Prosiding SAKTI (Seminar Ilmu Komputer dan Teknologi Informasi) Jurnal Riset dan Inovasi Peternakan Buletin Penelitian Tanaman Rempah dan Obat Wahana Peternakan Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Journal La Lifesci JUSTICIA SAINS: Jurnal Ilmu Hukum Jurnal Pendidikan Jurnal Hibrul Ulama Journal of Livestock and Animal Health (JLAH) Annales Bogorienses Berita Biologi
Ali Husni
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Veterinary Other

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Journal : ANNALES BOGORIENSES

 1 
In Vitro Induction Of Tetraploid Pummelo ’Nambangan’ (Citrus maxima (Burm.) Merr.) By Colchicine Treatment Using Germinated Seed, Shoot Tip And Cotyledonary Node As Explants Wulandari, Dyah Retno; Purwito, Agus; Susanto, Slamet; Husni, Ali; Ermayanti, Tri Muji
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i1.105

Abstract

Tetraploid citrus are important for interploidal hybridization to create triploid seedless citrus.  Colchicine is the most commonly used as antimitotic agent to induce polyploid plants.  Tetraploid induction by colchicine in Pummelo ‘Nambangan’ was conducted in vitro using different types of explants.  The aim of this research was to induce tetraploid pummelo ‘Nambangan’ by colchicine treatment using germinated seed, shoot tip and cotyledonary node as explants.  Tetraploid shoot induction was conducted by soaking germinated seeds, shoot tips and cotyledonary nodes in 0.1% colchicine for 1, 3 and 5 h.  Regenerant shoots were grown on MS medium and their growth was observed after four weeks in culture.  Ploidy level was determined using flow cytometry analysis.  Stomata density, length and width of stomatal guard cell were also recorded. The results showed that shoot elongation was inhibited by colchicine treatment.  Soaking of shoot tip explants in 0.1% colchicine for 1 h resulted in 66.66% of putative tetraploid shoots.  Compared to diploid shoots, tetraploids had lower stomata density but bigger in guard cell size. Keywords: colchicine, tetraploid, pummelo (Citrus maxima (Burm.) Merr.), flow cytometry, stomata,
Characterization Of Zygotic And Nucellar Embryo Of Six Indonesian Mango Cultivars Using Molecular Markers Fatimah, Fatimah; Husni, Ali; Kosmiatin, Mia; Karsinah, Karsinah; Baroya, Mushlihatun
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.762 KB) | DOI: 10.14203/ann.bogor.2016.v20.n2.69-75

Abstract

Breeding mangoes is a long term activity and complicated. One of difficulties is polyembryonic seeds. These factors make genetic improvement through conventional parental selection and breeding slow and unpredictable. Adoption of molecular markers and genomics-based breeding strategies will likely improve predictability and breeding efficiency. The objective of this study was to evaluate the occurrence of zygotic or nucellar in polyembryony mango cultivars Garifta Merah, Lalijiwo, Manalagi, Madu, Saigon Kuning, and Saigon Merah using RAPD and SSR markers. The type of embryo (zygotic or nucellar) was evaluated by comparing the exhibiting amplification patterns, if different from the mother plant considered as zygotic and identified as nucellar if they exhibited the same banding pattern as the mother plant. Out of the 16 SSR and 16 RAPD primers evaluated, 9 primers each amplified the largest number of allele and sharply defined band. Dendogram of the SSR primers divide into three groups while RAPD primers distinguish clearly into two major groups, zygotic group and nucellar group. Based on zygotic and nucellar size, number and position, indicating no relationship between the type of embryo and embryo size, number and the position. 
Co-Authors Adhitia, Fabian Agus Joko Santoso Agus Purwito Annisa, Ajeng Nur Antika, Dea Mela Ariesta, Wiwin Arif Qisthon Arzakiyah, Fina Badruttamam, Badruttamam Baroya, Mushlihatun Beninda, Adellia Budiman, Edy Budiyanto, Ferly Rahmat Chaireni Martasari Dakhlan, Akhmad Daniatur, Arya Desyandri Desyandri Dewi Sukma Dewi, Anggi Derma Tungga Dian Puji Rahayu Dian Septinova Diny Dinarti Disa, Putra Rama edy santosa, purnama Eka Rosyida Erwanto Erwanto Farida Fathul Fathur Rahmi, Atika fatimah Fatimah Febriyanti, Leni Nur Fitri Yulianti Fransiska Hamdani, M Dima Iqbal Hobir, NFN Ika Mariska Ilhamsyah, Alifudin Indonant, Hassem Muhamad Inka Aulia Karsinah - Karyanti ,, Karyanti Kirani, Wahyu Sasi Kohir, Muhammad Zahir Alwan Komang Trisna Wirakusuma KRISTIANTO NUGROHO, KRISTIANTO Kurniantha, Muhammad Aldi Kusuma Adhianto Liman Liman Liman, Liman M. Kosmiatin Madi Hartono Maemunah Maemunah Maliya, Mirwa Siti Maulidiyah, Salamatul Mia Kosmiatin Muhammad Habibi Muhammad Mirandy Pratama Sirat Muhtarudin Musthofa, Arfan Fahrizki Permata Sari, Lilis Pramana, Weldi Adi Pratama Adi Prayoga Pratama, Ridho PUJI LESTARI Purnama Edy Santosa Putra, Alvin Permana Putri Pramudita Putri, Elly Yani Medyas Raden Mohamad Herdian Bhakti Ramadani, Fajar Ratna Ermawati Reni Indrayanti Reza Ramdan Rivai Rosita Rosita Rr Riyanti, Rr Rudy Sutrisna Samliok Ndobe Saputri, Nenti Sari, Berliana Setio, Slamet Setyadi, Hario Jati Slamet Susanto Sukmadjaja, D. Sulastri Sulastri Sulistiawan, Dicky Syinwani, Billy M Taruk, Medi TRI JOKO SANTOSO Tri Muji Ermayanti Triyani Dewi Veithzal Rivai Zainal Veronica Wanniatie Widiasri, Ni Luh Putu Widiawati, Arlintia Wulandari, Dyah Retno Wulandari, Dyah Retno Zakirah Raihani Ya’la