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Electrode Distances Of Microbial Fuel Cell System On Salted Boiled Fish Processing Wastewater To Electricity And Pollution Load Bustami Ibrahim; Pipih Suptijah; Bagus Sukma Agung
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 3 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia 20(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.916 KB) | DOI: 10.17844/jphpi.v20i3.19813

Abstract

Microbial fuel cell (MFC) is a technology produce electricity by helping exoelectrogenic bacteria. Thetechnology can also utilize fishery processing wastewater as a media for bacteria to live, so it reduces organicpollution load in the wastewater. The purpose of this research was to identify the effect of electrodes distanceto electricity and water quality parameters of fisheries processing wastewater using MFC technology.. TheMFC system used was single chamber system. The distance between electrodes applied were 2 cm, 4 cmand 6 cm and the electrodes were made of stainless wire mesh coated with chitosan and active carbon.The results showed that electrodes distance affected to MFC electricity within salted boiled fish wastewatermedia. The average value of electric current during 48 hours observation on the distance of 2 cm, 4 cm and6 cm were 0.17±0.06 mA, 0.46±0.17 mA and 0.44±0.16 mA, respectively. Average values of electric voltageon the distance of 2 cm, 4 cm and 6 cm were 0.12±0.03 V, 0.34±0.07 V and 0.37±0.08 V, respectively. Theresearch also showed that MFC system can decrease average value of BOD 20.5%, COD 30.41%, and TAN21.2 % of salted boiled fish wastewater media.
ANTIOXIDANT ACTIVITY OF PROTEIN HYDROLYSATE PRODUCED FROM TUNA EYE (Thunnus sp.) BY ENZYMATIC HYDROLYSIS Dewi Mutamimah; Bustami Ibrahim; Wini Trilaksani
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 21 No 3 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.073 KB) | DOI: 10.17844/jphpi.v21i3.24736

Abstract

Tuna (Thunnus sp.) by-products from frozen loin and canning industry especially the eye is rich in proteins and in lipids consisting of polyunsaturated fatty acids (PUFA). That requires protective agent (antioxidant) to inhibit the oxidation naturally present and predicted to be protein peptides. Enzymatic hydrolysis of protein is an appropriate method to produce bioactive peptide with such nutraceutical/pharmaceutical function such as an antioxidant peptide. This study aimed to produce protein hydrolysate having a function as anwith an antioxidant activity from eye of tuna through enzymatic hydrolysis and determining the antioxidant activity by DPPH methods. Protein soluble content of tuna’s eye protein hydrolysate (TEPH) ranged from 59.98±0.130 to 94.90±0.002%. The degree of hydrolysis (DH) of TEPH was about 9.10±0.28 to 16.14±0.09%. The highest inhibition of DPPH radical scavenging activity was 93.57±0.05% (at 5 mg/mL) was obtained with a DH of 11.35±0.002% at the concentration 0.1% of papain for 6 hours hydrolysis. The IC50 value of was 1.08±0.008 mg/mL
Hydroxyapatite Production from Cuttlebone as Bone Scaffold Material Preparations Krisman Umbu Henggu; Bustami Ibrahim; Pipih Suptijah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 22 No 1 (2019): Jurnal Pengolahan Hasil Perikanan
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2244.688 KB) | DOI: 10.17844/jphpi.v22i1.25869

Abstract

The increasing production of cuttlefish has been associated with the increasing of by-product waste particularly cuttlebone. Cuttlebone is known to contain an inorganic element in form of calcium carbonate(CaCO3) which can be utilized as a source of calcium oxide (CaO) for hydroxyapatite synthesis. This study was aimed to determine the physicochemical characteristics of the cuttlebone and the optimum calcination temperature for CaO extraction and hydroxyapatite synthesis. This study was divided into three steps. Firstly, analysis of the cuttlebone physicochemical properties; secondly, extraction and characterization of the CaO with different calcination temperature (500°C, 600°C, 700°C for 6 hours); and thirdly, hydroxyapatite synthesis using a combination of hydrothermal method at 200°C 6 hours and different calcination treatments (800°C, 900°C, 1,000°C for 1 hour). The results showed that the cuttlebone contained moisture 3.54±0.11%,lipid 0.32±0.19%, protein 4.78±0.23%, carbohydrate 5.29±0.02%, and ash 89.61±0.26. The main element of the ash was CaCO3 aragonite characterized by the high absorption at wavelengths of 1,795; 1,507;1,083; 871; 713 and 700 cm-1. The calcination treatment of 700°C produced the highest amount of CaO. The hydroxyapatite produced with a combination of hydrothermal and calcination temperature 1,000°Chad calcium phosphate ratio (Ca/P) 1.66, crystalline level 90.10%, amorphous level 9.90% and particles morphology of rod-shaped.
Biofilm Density on the Electrode is Positively Correlated with the Bioelectricity of the Microbial Fuel Cell of Fisheries Wastewater Bustami Ibrahim; Uju Uju; Alvindo Chrisna Mukti
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 22 No 1 (2019): Jurnal Pengolahan Hasil Perikanan
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.376 KB) | DOI: 10.17844/jphpi.v22i1.25880

Abstract

Microbial fuel cell (MFC) is a bioreactor utilizing bacteria as electrocatalysts to convert bioenergy from biomass into electrical energy. The aim of this research were to determine the effects of the electrode distance on the bacterial density and the electrical value generated by the MFC as well as to evaluate the ability of MFC in reducing the pollutant. Single chamber MFC system with various electrode distances including 2 cm, 4 cm, and 6 cm were assembled. The wastewater of fish pindang processing was used as the mediumfor the MFC. The results showed that the distance had no effect on the biofilm density of the electrode and the reduction of the wastewater pollutant load. However, the distance affected the electrical value of theMFC. Biofilm density on the MFC electrode after 120 hours was 0.65-6.46 CFU/ cm2. The highest voltage was obtained from the 6 cm electrode distance with the voltage 0.38±0.01 V. Positive correlation (R2 = 0.99)between microbial density and electricity produced at the cathode was observed, but weak at the anoda (R2 = 0.47). The MFC system could decrease the BOD value up to 50.78% and COD up to 33.29%, however the TAN value was increased to 6 mg/L.
Characterization of Tuna Jerky with the Addition of Seaweed (Eucheuma cottonii) Flour Reni Lobo; Joko Santoso; Bustami Ibrahim
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 22 No 2 (2019): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.311 KB) | DOI: 10.17844/jphpi.v22i2.27678

Abstract

Fish jerky has hard texture which could make the appearance became less attractive to consumers. The aim of this study was to characterize tuna jerky with the addition of seaweed flour E. cottonii in order to increase acceptability by consumers. Result shown that seaweed flour characterization was consisted dietary fiber 73.95±0.45%, viscosity 107.02±0.51 cPs, gel strength 435.03±4.99 g/cm2, carageenan 54.56±0.18%, heavy metal Hg was <0.002 ppm, Pb was <0.004 ppm and Cd was 0.063±0.001 ppm, water content 12.63±0.08% and yield 6.07±0.08%. Based on research result, the additional of 2.5% E. cottonii concentration flour resulting high quality jerky with appearance value was 7.60, flavour was 7.67, texture was 5.7 and taste was 7.63 from 1-9 scales. Result from paired comparison test against commercial jerky (beef) resulting positive value, that means the quality of tuna jerky had better quality compared to commercial jerky and well accepted by panelist. Proximate analysis of tuna jerky shown that protein content 30.24±0.1%, water 11.32±0.02%, lipid 3.03±0.00% and ash 5.69±0.06%. Texture analysis results shown hardness 827.50±15.67, adhesiveness 0.09±0.02 and fracture 10.95±2.24.
Kinerja Membran Komposit Kitosan-Karagenan pada Sistem Microbial Fuel Cell dalam menghasilkan Biolistrik dari Limbah Pemindangan Ikan: Performance of Chitosan-Carrageenan Composite Membrane on Microbial Fuel Cell System in Producing Bioelectricity from Boiled Fish Wastewater Bustami Ibrahim; Uju; Agus Muhamad Soleh
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 23 No 1 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(1)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.142 KB) | DOI: 10.17844/jphpi.v23i1.31056

Abstract

Microbial fuel cell (MFC) merupakan suatu teknologi yang memanfaatkan mikroba untuk mendegradasi bahan organik dan anorganik menjadi energi listrik, dapat dilakukan menggunakan sistem satu bejana atau dua bejana. Sistem MFC dua bejana menggunakan membran penukar proton yang berfungsi untuk mengalirkan proton yang dihasilkan dari ruang anoda ke ruang katoda, salah satu alternatif membran yang digunakan yaitu komposit kitosan-karagenan. Penelitian ini bertujuan untuk menentukan perbandingan komposit kitosan-karagenan sebagai membran penukar proton pada MFC, menentukan kinerja MFC dalam menghasilkan elektrisitas, serta menentukan kinerja penurunan beban polutan limbah cair pada MFC. Nilai elektrisitas MFC diukur menggunakan multimeter dengan parameter yang diuji adalah tegangan listrik, serta arus listrik. Parameter uji yang digunakan untuk mengukur penurunan beban polutan limbah cair adalah chemical oxygen demand (COD), biologycal oxygen demand (BOD) dan total amonia nitrogen (TAN). Membran komposit kitosan-karagenan dibuat dengan perlakuan perbedaan komposisi kitosan dan karagenan 1:1; 1,5:1; 3:1 (v/v). Perbedaan rasio kitosan dan karagenan pada membran komposit kitosan-karagenan memberikan pengaruh terhadap sifat mekanik membran, nilai elektrisitas MFC, serta beban polutan cair pada MFC. Membran komposit kitosan-karagenan dengan perbandingan 1:1 menghasilkan nilai konduktivitas proton tertinggi sebesar 1,15x10-3 S/cm, kuat tarik tertinggi 7,047 MPa, tegangan listrik 0,97 V, arus 7,02 mA, serta daya listrik 6,84 mW. Nilai COD, BOD, serta TAN limbah cair pemindangan ikan mengalami penurunan sebesar 90%, 76% dan 32%.
ISOLATION AND SCREENING OF ENDOPHYTIC MARINE FUNGI FOR CELLULASE PRODUCTION Aulia Andhikawati; Yulia Oktavia; Bustami Ibrahim; Kustiariyah Tarman
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.895 KB) | DOI: 10.29244/jitkt.v6i1.8643

Abstract

Cellulolytic fungi are potentially useful in biotechnology. Decomposition of substrates containing cellulose can be applied in bioethanol and enzymes production. Fungi are one group of microorganisms that can decompose cellulase materials.  This study aimed to obtain potential cellulolytic fungi isolated from marine habitat. Screening of the fungi was performed using CBM media with different salinity. Six isolates were tested quantitatively for cellulolytic activity using congo red staining. Cellulolytic index of algicolous and manglicolous fungi were lower than those of EN isolate, Veronea sp. KT19, and sponge associate (SMH). Fungi cultured in freshwater and seawater media possessed higher cellulolytic index in compared to those of cultures in 3% NaCl medium. However, freshwater and seawater media showed no significant effect. Cellulolytic index of EN isolate, Veronaea sp. KT19, and SMH after six days of incubation were 1.357; 1.267, and 1.278, respectively. All six isolates potentially produced cellulase in all medium tested. Keywords: Cellulolytic, cellulose, endophytes, enzyme
KARAKTERISASI PEPTON IKAN HASIL TANGKAP SAMPINGAN TIDAK LAYAK KONSUMSI SEBAGAI SUMBER NUTRIEN PERTUMBUHAN MIKROORGANISME Tati Nurhayati, Bustami Ibrahim, Pipih Suptijah, Ella Salamah, Risa Nurul Fitra, Eska Rizky wiji Ast
Jurnal Teknologi Industri Pertanian Vol. 25 No. 1 (2015): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Spoiled by-catch fish (HTS) has a high protein content, so that is potential to be used as peptone raw material. This research aimed to produce peptone from spoiled by-catch fish and to apply it as nutrient source of bacteria and yeast growth. Peptone was produced by the addition of 0.3% papain enzyme 5 hours hydrolysed process. The result showed that peptone from by-catch fish had protein of 71.39%, solubility of 99.96%, nitrogen of 11.42%, α-Amino free nitrogen of 1.76%, AN/TN ratio of 15.41%, salt of 7.82% and pH of 7.1. The results ofthe analysis of the specific growth rate (μ max) and the average growth rate indicated that the media containing peptone HTS did not give a better growth effect on Staphylococcus aureus and Sacharomyces cereviceae than that grown on media without peptone and commercial peptone. Escherichiacoli grown in media containing peptone HTS was not consumable due to the lower values of μ max and the average growth rate.Keywords: by-catch fish, growth nutrient, hydrolysis, peptone
PRODUKSI BIODIESEL DARI BIOMASSA Chlamydomonas sp. ICBB 9113 DIKULTIVASI MENGGUNAKAN MEDIA YANG MURAH: EFEKTIFITAS DARI BEBERAPA METODE EKSTRAKSI Patmawati Patmawati; Bustami Ibrahim; Iriani Setyaningsih; Untung Sudadi
Widyariset Vol 17, No 2 (2014): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.292 KB) | DOI: 10.14203/widyariset.17.2.2014.269-276

Abstract

The main challenges to overcome in biodiesel production from microalgae are lower oil yield, as compared to those derived from plant and animal biomass, and expensive culture media. This work was aimed to compare the effectivity of three extraction methods differed in solvent used, e.g. n-hexane (N-hex), ethanol (Eth), and mixture of chloroform-methanol-water (CMW), to extract crude lipid and biodiesel from dry biomass of Chlamydomonas sp. ICBB 9113. This microalgae was cultivated in a cheap culture media using N and P soil fertilizers as nutrient sources. The results showed that, by using N-hex, Eth, and CMW methods, it could be extracted, respectively, 0.06%, 4.51%, and 20.45% crude lipid, and 384.2, 1333.8, and 2430.6 mg/100g biodiesel. The fatty acid profile of the studied microalgae biomass was: C8:0 (0.11%), C10:0 (0.09%), C14:0 (7.70%), 16:0 (1.39%), C18:0 (0.85%), C14:1 (5.12%), C16:1 (7.09%), C18:1 (8.28%), C18:2 (12.80%), and C18:3 (42.57%). Fatty acid characterization showed that Chlamydomonas sp. ICBB 9113 was dominated by C18: 3 and C16:0. Therefore, these microlagae were suitable to be used as raw material for biodiesel production to substitute the conventional fuel.
PRODUKSI BIODIESEL DARI BIOMASSA Chlamydomonas sp. ICBB 9113 DIKULTIVASI MENGGUNAKAN MEDIA YANG MURAH: EFEKTIFITAS DARI BEBERAPA METODE EKSTRAKSI Patmawati Patmawati; Bustami Ibrahim; Iriani Setyaningsih; Untung Sudadi
Widyariset Vol 17, No 2 (2014): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.292 KB) | DOI: 10.14203/widyariset.17.2.2014.269-276

Abstract

The main challenges to overcome in biodiesel production from microalgae are lower oil yield, as compared to those derived from plant and animal biomass, and expensive culture media. This work was aimed to compare the effectivity of three extraction methods differed in solvent used, e.g. n-hexane (N-hex), ethanol (Eth), and mixture of chloroform-methanol-water (CMW), to extract crude lipid and biodiesel from dry biomass of Chlamydomonas sp. ICBB 9113. This microalgae was cultivated in a cheap culture media using N and P soil fertilizers as nutrient sources. The results showed that, by using N-hex, Eth, and CMW methods, it could be extracted, respectively, 0.06%, 4.51%, and 20.45% crude lipid, and 384.2, 1333.8, and 2430.6 mg/100g biodiesel. The fatty acid profile of the studied microalgae biomass was: C8:0 (0.11%), C10:0 (0.09%), C14:0 (7.70%), 16:0 (1.39%), C18:0 (0.85%), C14:1 (5.12%), C16:1 (7.09%), C18:1 (8.28%), C18:2 (12.80%), and C18:3 (42.57%). Fatty acid characterization showed that Chlamydomonas sp. ICBB 9113 was dominated by C18: 3 and C16:0. Therefore, these microlagae were suitable to be used as raw material for biodiesel production to substitute the conventional fuel.
Co-Authors . Heriyanto . Prantommy . Uju . Uju Ade Komalasari Suhendar Adenia Cahyatie Aprillia Agus Muhamad Soleh Ahmad Zahid Aldo Reshwara Alvi Nur Yudistira Alvindo Chrisna Mukti Anna C. Erungan As Syaffa Amalia Adha Asadatun Abdullah Aulia Andhikawati Bagus Sukma Agung Dadan Heryada Wigenaputra Dadi Rochnadi Sukarsa Desi Listiana Desniar - - Dewi Mutamimah Dwi Winarsih Ella - Salamah ETI KURNIAWATI Farah Nurjannah Farell Pramuja Fariz Pari, Rizfi Giri Rohmad Barokah Henggu, Krisman Umbu Hijrah Amin Hirda Umayyah I Dewa Made Subrata Iffani Nabila Zain Irama Dramawanti Pamungkas Iriani Setyaningsih Irma Irma Jannah, Arum Jayanti, Feby Dwi Joko Santoso Kurniawan, Farhan Kustiariyah Tarman Laode Muhamad Hazairin Nadia Linda Aryanti Maglory Siburian Mandela, Risco Mohammad Haryst Daryaly Mudji Ana Yanti Muhamad Reza Fahlepi Muhammad Zaki Dama Nazihah, Nilna Tsabita NONI DWI SARI Nur Shasqia Malewa Nurhaliza, Ghefira Nurjanah Nurmaida Nurmaida Nurul - Hak Patmawati Patmawati Pipih Suptijah Pramesti, Nabilla Adhiany Pratiwi, Widya Prapti Purwadi, Wiwik Raffiq, Fathurrizqi Ramadhan, Rahmat Agung Ravellino Fahlepi Reni Lobo Ridho Orilda Rizki Tri Kurnia Ramadhan Roni Nugraha Rudy Nistibaskara safrina dyah hardiningtyas Safrina Dyah Hardiningtyas Setyarini, Dian Shabrina Itsnaini Oktafira Sidik, Adi Permana Sita Permata Sari Slamet Hermanto Steffen Sugeng Heri Suseno Surya Herlambang, Firhan Swastikawati, Ayu Setiti syeila - rosmalawati TATI NURHAYATI Theresia Puspita Arumsari Uju Uju Uju Uju Uju Uju Untung Sudadi Wini - Trilaksani Yogaswara, Ghema Yulia Oktavia YULYA FITRIA Zhalindri Noor Adjani