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Journal : Jurnal Biologi Tropis

Organoid Culture Applications: Mini Systematic Review Rilwan Efendi; Puti Khairunnajwa Amar; Resti Rahayu; Putra Santoso; Zozy Aneloi Noli; Rita Maliza
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5387

Abstract

Recent scientific developments in the stem cell field have made it possible to create complex organoids, or structures that resemble complete organs, in vitro. In the majority of these methods, stem cells produced from stem cells or tissue progenitors are allowed to self-organize into three-dimensional (3D) structures using culture systems in three dimensions. The purpose of this study is to ascertain the use of organoid culture in the area of health. This kind of study employs the literature review methodology. The Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) criteria are referenced throughout the stages of the literature review performed in this study. The four processes that make up this level are identification, screening, eligibility, and acceptance. These discoveries showing that human stem cells and patient-derived pluripotent stem cells can be used to generate organoids open up a wide range of opportunities for modeling and disease development, toxicological research and medication discovery, and the field of regenerative medicine. Here, we discuss some of the most significant recent advancements in 3D human organoid production as well as the field's historical advancements. Finally, we discuss present restrictions and provide illustrations of how organoid technology may be used in the healthcare industry.
Twenty-Five Years Research on Micropropagation of Stevia and Curcuma sp. and Improving Secondary Metabolites using Precursor-elicitor in vitro: A Review Delfi, Shyla Aulia; Putri, Suci Indah; Santoso, Putra; Idris, Muhammad
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8882

Abstract

In vitro culture technique is an effective method for plant propagation to overcome the limitations of conventional cultivation. This method is used to improve accumulation of plant secondary metabolites. The purpose of this study is to review the development of micropropagation and improving secondary metabolites in Stevia rebaudiana and Curcuma plants through the application of precursors and elicitors. The systematic literature review is used to analyse scientific articles or publications from 2000 to 2025 obtained from various online databases using relevant keywords. The results showed that Stevia was more studied when compared to Curcuma due to its high economic value, with significant impact on the study of improving accumulation of stevioside and rebaudioside production through several elicitor and precursors. In Curcuma, beside in vitro culture for mass propagation, the response to in vitro treatment of elicitor dan precursors were varied, but some precursors such as phenylalanine were shown to improve curcuminoid accumulation. In conclusion, tissue culture techniques and the use of elicitor and precursor for improving secondary metabolites accumulation have the potential impact to support mass propagation, production and sustainable optimization of plant bioactive compounds.
Literature Review: Analysis of Cyanogenic Compounds in Plants Noli, Zozy Aneloi; Asih, Enda Tarni; Pasha, Gusti Ari Afrilya; Siagian, Marhamah; Adelia, Sabbrina; Putri, Suci Indah; Santoso, Putra
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9417

Abstract

Cyanogenic glycosides are hydrocarbon compounds that can bind to CN groups and sugars. Certain plants, notably higher plants, can perform cyanogenesis, forming cyanogenic glycosides as by-products of biochemical reactions. From a medical perspective, cyanide is known to negatively impact health, particularly the respiratory system, as it binds oxygen in the blood to toxic compounds. Symptoms of cyanide poisoning from food consumption include dizziness, weakness, vomiting, throat inflammation, and abdominal cramps. Acute poisoning and death have been reported from consuming food containing 50 – 100 mg of cyanide. This review utilizes a literature study method, focusing on national journals from the past decade accessed via Google Scholar. The findings identify five plant species containing cyanogenic glycosides: cassava, bamboo shoots, cassava leaves, raw Pangium edule seeds, and Dioscorea hispida tubers. Cassava, in particular, contains linamarin, a cyanogenic glycoside found in all parts of the plant, with bitter varieties generally having higher toxin levels.
Effects of Low Protein Diet on Body Weight and Hematological Parameters in Rats Zikrah, Azizah Amatu; Santoso, Putra; Maliza, Rita
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7066

Abstract

Malnutrition is a condition in which the body is deficient or excessive in macronutrients and micronutrients such as carbohydrates, fats, proteins and vitamins. Malnutrition causes changes in body weight and blood tissue that inhibit the development of hematopoietic cells, resulting in anemia, leukopenia and bone marrow hypoplasia. This study aimed to examine the effect of feeding a diet with a protein level of 12% and 20 % for 6-week to Wistar rats. The parameters seen are the impact on body weight and selected hematological parameters included white blood cell, monocyte, lymphocyte, granulocyte, hemoglobin and mean corpuscular volume. This study found that rats fed with 12% protein showed significant differences in body weight and several hematological parameters such as monocytes, lymphocytes, hemoglobin and mean corpuscular volume compared to normal.
Black Soldier Fly (Hermetia illucens L.) Larvae Oil Ointment to Accelerate Incision Wound Healing in Mice Afriani, Yona; Rahayu, Resti; Santoso, Putra
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7272

Abstract

Larvae of the Black Soldier Fly (BSF) are a natural material that has the potential to become a new alternative for accelerating wound healing due to their nutritional content. This study aims to analyze the potential of BSF larvae oil ointment in accelerating the healing of incisional wounds in mice. The study was conducted as an experimental research with six treatments and four repetitions, including a control group without treatment, ointment base, gentamicin ointment, BSF larvae oil ointment at 5% concentration, BSF larvae oil ointment at 10% concentration, and BSF larvae oil ointment at 15% concentration. The parameters observed included the percentage of wound healing, area under the curve (AUC) values, and anti-inflammatory efficacy percentage. The results showed no significant differences between groups based on statistical analysis (P>0.05) for the observed parameters. However, the 5% BSF larvae oil ointment showed a 100% healing percentage on the fourteenth day. Based on AUC values and anti-inflammatory efficacy percentage, the 5% BSF larvae oil ointment had values close to commercial gentamicin ointment. This indicates that treatment with BSF larvae oil ointment, especially at the lowest dose, plays a role in accelerating incisional wound healing and has great potential to be developed as a commercial ointment in the future.
Mentawai Taro Corm Flour’s Benefits on Hyperglycemia and Pancreas Histopathology in Diabetic Mice Aini, Wardatul; Santoso, Putra; Rahayu, Resti
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7763

Abstract

Diabetes mellitus (DM) is among the most severe health issues globally. This study aimed to determine the beneficial effects of Mentawai taro (Colocasia esculenta var. Mentawai) in managing hyperglycemia and attenuating inflammation in pancreatic ß-cells in alloxan-induced diabetic mice. Twenty-one adult male mice were divided into three groups: healthy (non-DM), alloxan-induced DM, and DM mice fed with a diet containing 25% Mentawai taro flour. The treatments were administered for four consecutive weeks. Various parameters, including blood glucose levels, glucose tolerance, and insulin tolerance, were assessed, alongside microscopic examination of pancreatic histology. The results demonstrated that supplementation with 25% Mentawai taro corm significantly lowered fasting blood glucose levels (**P<0.01) and improved glucose tolerance (*P<0.05) and insulin tolerance (*P<0.05) compared to untreated diabetic mice. Mentawai taro corm also ameliorated pancreatic degeneration, as indicated by a larger islet of Langerhans area, a higher total cell number per islet, and a significantly lower number of degenerated cells in pancreatic tissue (**P<0.01). In conclusion, supplementing Mentawai taro corm at 25% in the diet could effectively help manage diabetic issues, including hyperglycemia and pancreatic degeneration.
Physical Quality and Organoleptic Testing of Golden Apple Snail Pomacea canaliculata) Mucus Gel as a Burn Treatment Fadhilah, Diana; Nadjmir, Mikail; Adilla, Ardis Trianita; Santoso, Putra; Maliza, Rita
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8023

Abstract

Golden Apple Snail (Pomacea canaliculata) secretion has traditionally been used for wound healing due to its high protein content, which promotes cell regeneration and inhibits inflammation. This study aims to utilize this secretion to formulate a topical gel for burn treatment, providing a controlled use for the abundant, invasive species in agricultural areas. Gel formulations with varying secretion concentrations (10%, 15%, and 20%) were evaluated for organoleptic properties, homogeneity, pH, adhesion, spreadability, and viscosity. Results indicated that all formulations were homogeneous and had pH values within the acceptable range (4.5-6.5). The 15% secretion gel showed the highest user acceptance and optimal balance between spreadability and adhesion, but all formulations had higher than standard viscosity and lower spreadability. Adjusting carbopol concentration is recommended to improve the gel's physical properties. The 15% formulation is the most promising for effective topical application.
Evaluation of Anti-inflammatory Effects of Extract Maggot Black Soldier Fly (Hermetia illucens L.) in vitro study Rahayu, Resti; Efendi, Rilwan; Santoso, Putra
Jurnal Biologi Tropis Vol. 24 No. 2b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2b.8100

Abstract

Inflammation is the body's normal protective response, but it is often an over-response that can cause tissue damage. Commercial synthetic anti-inflammatory drugs of steroidal and non-steroidal groups have high side effects. So that alternative anti-inflammatory drugs are needed that have low side effects. Maggot black soldier fly is a type of insect that has begun to be widely studied to utilize its bioactives as anti-inflammatory, one of which is because it is high in protein. Therefore, this study aims to explore the anti-inflammatory properties of black soldier fly maggot extract. Anti-inflammatory testing of maggot extract was carried out using an in vitro heat-induced protein denaturation assay, with diclofenac sodium serving as a commercial drug reference. It was found that maggot extracted with 100% methanol was 5 times more effective than maggot extracted with other methanol concentrations. Amino acid analysis showed increased levels of alanine and arginine, which are believed to contribute to the inhibition of protein denaturation. Thus, maggot extracted with 100% methanol showed better potential as an anti-inflammatory drug.
Literature Review: The Potential of Phenolic Compounds as Allelopathic Agents in Mangifera indica Noli, Zozy Aneloi; Nurhafitri, Amanda; Puspita, Ayumi Rizci; Putri, Mellanie Alia; Santoso, Putra
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8271

Abstract

Phenolic compounds are a group of secondary metabolites identified as having significant roles in various ecophysiological processes, including allelopathic activity. In Mangifera indica (mango), phenolic compounds contribute to chemical interactions between plants by releasing allelopathic substances that influence the growth and development of surrounding plant species. This study aims to review the potential of phenolic compounds in M. indica as allelopathic agents, focusing on identifying dominant types of phenolic compounds, allelopathic mechanisms, and their ecological impacts on plant communities. The research method employed is a literature review involving a search for relevant sources on Google Scholar covering the period from 2010 to 2024. Based on a review of various studies, it has been demonstrated that phenolic compounds in M. indica possess significant potential to inhibit the growth of competitors and can be utilized in environmentally friendly agricultural management.
Literatur Review: Analysis of Essential Oil Secondary Metabolite Content in Several Plants Noli, Zozy Aneloi; Delfi, Shyla Aulia; Zulkarnain, Alivia; Syabila, Hutri Dinda; Rusiati, Anisa Rahman; Santoso, Putra
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8513

Abstract

Indonesia’s rich plant biodiversity offers a wide array of secondary metabolites, including essential oils, known for their antioxidant, antifungal, and antibacterial properties. This review article examines the secondary metabolite composition of essential oils from various plant species by synthesizing findings from existing literature. The review article highlights the presence of diverse compounds, including terpenes, phenols, and alkaloids, with variations observed between species—for instance, limonin in citrus, linalool in ylang-ylang, and eugenol in cloves. Commonly utilized methodologies, such as steam distillation and GC-MS analysis, discuss their effectiveness in characterizing essential oil components. The findings underscore the extensive potential of crucial oils for applications in health, food, and cosmetic industries, providing a foundation for future research and practical innovations.
Co-Authors . Mansyurdin Adelia, sabbrina Adilla, Ardis Trianita Afriani, Nita Afriani, Yona Aini, Wardahtul Aini, Wardatul Alimuddin Tofrizal Asful, Ferdhinal Ashrifurrahman, Ashrifurrahman Asih, Enda Tarni Aszareta, Muhammad Andoni Aziz, M. Abdul Belahusna, Dinda Fadhilah Bramadi Arya Dahelmi Dahelmi Delfi, Shyla Aulia Dinda Fadhilah Belahusna Efendi, Rilwan Efrizal Efrizal Eli Ratni Elni Fatimah Elni Fatimah Eti Yerizel Fadhilah, Diana Fadil, Muhammad Syukri Fadilaturahmah Fadilaturahmah Fadillah Fadillah Fadillah Fajri Hidayat Feskaharni Alamsjah Firman Syukri Hamdi Ibrahim, Muhammad Hari Marta Saputra Hasmiandy Hamid Henny Herwina Hermeria, Noveriza Husna Yetti Idris, Muhamma Ilham Samudra, Muhammad Jabang Nurdin Janra, Muhammad Nazri Jasmi Jasmi Jasmi Jefrial Santoso Maliza, Rita Marchellino Irwan, Reziq Maya Sari Miftahul Ilmi Muhammad Afif Muhammad Idris Muhammad Idris Muhammad Idris Muhammad Nazri Janra Nabilah Rahmachila Azura Nadira Nurul Fathiyah Nadjmir, Mikail Nazri Janra, Muhammad Netti Marusin Netti Marusin Nofrita Nofrita Nofrita Nofrita Nurhafitri, Amanda Pasha, Gusti Ari Afrilya Petrovsky, Ivan Pratama Elisa, Tasya Putri Pratama, Raihan Anugrah Puspita, Ayumi Rizci Puti Khairunnajwa Amar Putri Pratama Elisa, Tasya Putri, Mellanie Alia Putri, Suci Indah Putri, Syntia Mai r maliza, rita Rarastoeti Pratiwi Rauza Sukma Rita Resti Rahayu Resti Rahayu Ridho, Muhammad Syifa'ur Rilwan Efendi Robby Jannatan Rusiati, Anisa Rahman Sayati Mandia Siagian, Marhamah Sinurat, Arthauly Yopita Syabila, Hutri Dinda Syaifullah Syaifullah Syakira Tiara Rezvi Syamsuardi Syamsuardi Tofrizal Tofrizal, Alimuddin Wellyalina, Wellyalina Wilson Novarino Yona Afriani Yulmira Yanti Zaini Zaini Zikrah, Azizah Amatu Zozy Aneloi Noli Zulkarnain, Alivia