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All Journal HAYATI Journal of Biosciences VALENSI Bionatura Indonesian Journal of Applied Sciences Microbiology Indonesia Majalah Kedokteran Bandung Indonesian Journal of Pharmaceutical Science and Technology Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia JURNAL SAINS INDONESIA Jurnal Pendidikan Kimia (JPKim) Indonesian Journal of Chemistry Pharmaceutical Sciences and Research (PSR) Jurnal Sains dan Teknologi Farmasi Indonesia Kimia Padjadjaran
Iman Permana Maksum
Departemen Kimia, FMIPA, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Journal : VALENSI

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The Effect of Single Co-expression of The DnaK-DnaJ-GrpE and GroEL/ES Chaperones and Their Combination on Expression Intein-pretrombin-2 in Escherichia coli ER2566 Iman Permana Maksum; D Agus Yusuf Wildan; Khomaini Hasan; Toto Subroto
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 6, No. 1, May 2020
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.174 KB) | DOI: 10.15408/jkv.v6i1.11333

Abstract

The use of recombinant thrombin in the manufacture of fibrin glue allows diseases contamination to be avoided. However, the expression of recombinant protein in E. coli still has a disadvantage of the formation of inclusion bodies, so it needs to be minimized by co-expression of chaperones. Therefore, the aim of this study was to determine the effect of single DnaK-DnaJ-GrpE and GroEL/ES chaperone expression and their combination on the expression of intein-pretrombin-2Ti,pH on E. coli ER2566. The method started with isolation of pTWIN1-prethrombin-2Ti,pH and pG-KJE8 from E. coli TOP10F' and DH5α respectively, the co-transformation of the expression host E. coli ER2566 using pG-KJE8 and pTWIN1-prethrombin-2Ti,pHvectors, the chaperone co-expression was induced using L-Arabinosa before IPTG induction and cell culture growth was incubated at 22 oC. The expression products were characterized by using Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The results of the co-expression of chaperone showed that the number of soluble fraction was higher than the one without co-expression of chaperone. In addition, the co-expression of chaperone using pG-KJE8 in intein-prothrombin-2Ti,pH expression was sufficient using tetracycline as an inducer.
PCR Multipleks untuk Identifikasi Mycobacterium tuberculosis Resisten terhadap Isoniazid dan Rifampisin pada Galur Lokal Balai Laboratorium Kesehatan Provinsi Jawa Barat Iman Permana Maksum; Suhaili Suhaili; Rizki Amalia; Dian Siti Kamara; Saadah Dian Rachman; Rifky W. Rachman
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 4, No. 2, November 2018
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (649.2 KB) | DOI: 10.15408/jkv.v4i2.7226

Abstract

The incidence of multidrug-resistant tuberculosis (MDR-TB) cases has become the biggest source of the problem in the effort to eradicate tuberculosis (TB) disease in Indonesia. MDR-TB is a resistant TB bacteria to the two, at least, first-line TB drugs, e.g., rifampin and isoniazid. Unfortunately, the current diagnostics methods to identify the MDR-TB are still slow, unspecific, and inaccurate. The purpose of this study is to identify the isoniazid- and rifampin-resistant M. tuberculosis (local strain Balai Laboratorium Kesehatan Provinsi Jawa Barat) by using multiplex PCR method. The TB bacteria colonies were cultivated in Middlebrook 7h9 broth media, which followed by the isolation of chromosomal DNA. The best PCR condition was achieved by optimizing the annealing temperature, the concentration of magnesium chloride, and a number of the cycle. Multiplex PCR was conducted with inhA1-inhA2, rpoB1- rpoB2, katG1- katG2, and B1-B2 pair primers. Furthermore, the PCR product was characterized on 2% gel agarose electrophoresis which stained by using ethidium bromide. The result showed that isoniazid- and rifampin-resistant M. tuberculosis sample could be identified using multiplex PCR, producing DNA fragments with a size of 71 bp, 124 bp 186 bp, and 200 bp. A non-MDR-TB only produced one DNA fragments with a size of 200 bp. Therefore, it can be concluded that MDR-TB and non-MDR-TB can be distinguished using multiplex PCR with a combination of four pair primers.  
Co-Authors Abubakar Sidik Abubakar Sidik Ace Tatang Hidayat Ade Sholeh Hidayat Agus Safari Agus Safari Ahmad Fariz Maulana Alifa I. Nabila Anne Carolina Astrid, Dewi Azizah, Mamlikatu Ilmi Budiantoro, Ogi Carolina, Anne D Agus Yusuf Wildan DESSY NATALIA Dewi Astriany Dewi Astrid Dian Siti Kamara Diandra Firdiani Utami DWIRYANI ARIZONA Effendi, Syulastri Euis Julaeha Euis Julaeha Febriani Aji Kusuma, Sherlynda Febrianti, Inky Hidayat, Ade Sholeh Iman Rahayu Inky Febrianti KHOMAINI HASAN Khomairi Hasan Kusumawardhani, Shinta Mamlikatu Ilmi Azizah Mamlikatu Ilmi Azizah Muhammad Yusuf Muhammad Yusuf Mulyani, Dinda Exelsa Mulyani, Rahmaniar Mulyasari, Rita Murniaty Simorangkir Murniaty Simorangkir Nida Yumna O Suprijana O Suprijana, O Ogi Budiantoro Pertiwi, Wulan Purba Upay R. Ukun M.S. Soedjanaatmadja Rachman, Saadah Diana Rahmaniar Mulyani Rahmaniar Mulyani Rifky W. Rachman Rita Mulyasari Riyona Desvy Pratiwi Rizki Amalia Ryan Adibagus Haryanto Saadah Dian Rachman Saadah Diana Rachman Safri Ishmayana Sari Agung, Syennie Saronom Silaban Saronom Silaban SARONOM SILABAN Saronom Silaban Setiawan, Topan Shabarni Gaffar Sheila Destiani Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro SOETIJOSO SOEMITRO Soetijoso Soemitro Sriwidodo B, Sriwidodo Sriwidodo Sriwidodo Sriwidodo Sriwidodo Sriwidodo, . Suhaili Suhaili Sunan M. Alpiansyah Sutarya Enus Sutarya Enus SUTARYA ENUS Sutarya Enus TATI NURHAYATI Toto Subroto Upay, Purba Wanda Destiarani Wulan Pertiwi Yanyan Mulyana Yeni Wahyuni Yeni Wahyuni Hartati