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All Journal Jurnal Kedokteran Brawijaya e-Journal Pustaka Kesehatan Jurnal Kesehatan STOMATOGNATIC- Jurnal Kedokteran Gigi Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Journal of Dentomaxillofacial Science BIOEDUKASI Odonto dental journal
Happy Harmono
Bagian Biomedik, Fakultas Kedokteran Gigi, Universitas Jember, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Library & Information Science Medicine & Pharmacology Nursing Public Health

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IL-17 plasma levels and erythrocyte sedimentation rate on oral candidiasis animal model Erna Sulistyani; Iin Eliana Triwahyuni; Happy Harmono; Lisa Miftakhul Janna; Saikha Adila Azzah; Muchamad Ziyad Afif; Ainunnusak Ayuningtyas
Dental Journal Vol. 55 No. 4 (2022): December 2022
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v55.i4.p209-214

Abstract

Background: A study with female animal models is important because the system immune of females is remarkably different from the male because of interaction between sex hormone and immune system. Interleukin-17 (IL-17) plays an important role in immune response toward Candida albicans (C. albicans) infection and Erythrocyte Sedimentation Rate (ERS) is an easy and sensitive test to assess the inflammation. Purpose: This study aimed to evaluate C. albicans infection, analyse the IL-17 levels and ESR in a female animal model of oral candidiasis. Methods: Female Wistar rats were used as oral candidiasis animal model. The rats divided into three groups (pre-treatment group (P0), 5th-day post-treatment group (P1) and 8th-day post- treatment group (P1). Each group consists of six rats. After the adaptation period, the P0 was sacrificed. The drinking water of the P1 and P2 was added tetracycline HCl 500mg /1L. On the day before and after inoculation C. albicans, Methylprednisolone was injected. Ten minutes before inoculation with 0.3 ml C. albicans 9.4 x 107/mL, rats were sedated by CPZ 0.7 mg IM. The rats in P1 group were sacrificed after five days and in P2 were sacrificed eight days after inoculation. The IL-17 plasma levels measured by enzyme-linked immunosorbent assay (ELISA), decreased on the 5th day but not on the 8th-day post-treatment. The obtained data were analyzed by parametric and non-parametric tests according to normality and homogeneity of the data with p<0.05. Results: The colony forming unit (CFU) of C. albicans collected over the mouth on increased almost 8-fold and on 8th-day post-treatment almost 3-fold compared with pre-treatment. The ESR increased on the 8th day but not on the 5th-day post-treatment. Conclusion: The IL-17 level was decreased on the 3rd day, ESR increased on eight days after inoculation of C. albicans in female rats’ model of oral candidiasis.
EFFECTIVENESS OF ROBUSTA COFFEE BEAN EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN POST-GINGIVECTOMY WOUND HEALING Tantin Ermawati; Happy Harmono; Dania Kartikasari
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (866.887 KB) | DOI: 10.30659/odj.8.1.45-53

Abstract

Background: Gingivectomy is a periodontal surgical procedure that eliminates excess gingival tissues by excision, usually to remove a gingival pocket which leads to scars and recontours the gingival to its proper anatomical form. Wound healing is a process to restore the missing functions and structures of a tissue due to injuries or inflammations. Robusta coffee bean contains many bioactive compounds that can accelerate the wound healing process. The study aimed to determinate the effectiveness of robusta coffee bean extract gel with concentration 40%,50%,60% on collagen fibers density in post-gingivectomy wound healing Method: 36 Wistar rats were divided into 9 groups: control, gingivectomy without therapy, gingivectomy +40% robusta coffee bean extract gel, gingivectomy +50% robusta coffee bean extract gel, and gingivectomy+60% robusta coffee bean extract gel. The last 4 groups were divided again into day 3 and 7 euthanasia. The results were processed by Trichrome Mallory to visualized the collagen fibers and then measured by ImageJ software.Result: Robusta coffee beans extract gel therapy with concentration 40%, 50% and 60% had higher average collagen fibers density compared to group without therapy.Conclusion: Robusta coffee bean extract gel increased the density of collagen fibers in post-gingivectomy wound healing
Ekspresi BMP2 dan TRAF6 pada Jaringan Periodontal setelah Pemberian Ekstrak Kopi Robusta selama Pergerakan Gigi Ortodonti Herniyati Herniyati; Leliana Sabdra deviade Putri; Hestieyonini Hadnyanawati; Happy Harmono
Pustaka Kesehatan Vol 11 No 2 (2023): Volume 11 No.2, 2023
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v11i2.38029

Abstract

Orthodontic tooth movement occurs due to mechanical stimulation followed by alveolar bone and periodontal ligament remodeling. Bone remodeling is the process of bone resorption in areas of tension and bone formation in areas of tension. Various techniques and drugs are used to speed up orthodontic treatment. This study aims to analyze the expression of BMP2 and TRAP6 on orthodontic tooth movement after the administration of Robusta coffee extract. Twenty-four Wistar rats were divided into 4 groups: C1 (2 weeks) and C2 (3 weeks) groups: rats were given orthodontic mechanical force (OMF) and T1 (2 weeks) and T2 (3 weeks) groups rats were given OMF and Robusta coffee extract. OMF in rats was performed by moving the maxillary right permanent molars mesially with a Niti closed coil spring. Observations were made on the 15th and 22nd day by immunohistochemical examination to determine the expression of BMP2 and TRAP6. Administration of Robusta coffee extract for 2 weeks and 3 weeks increased BMP2 and TRAP6 expression (p <0.05). Administration of Robusta coffee extract increased the expression of BMP2 and TRAP6 in the alveolar bone during orthodontic tooth movement.
The Potency of Polyphenols Extract of Robusta Coffee Bean (Coffea robusta) on COX-2 Inhibition in Neutrophil Cells Tantin Ermawati; Qurratul Aini Wahyu Pratiwi; Zahara Meilawaty; Happy Harmono
Jurnal Kedokteran Brawijaya Vol. 32 No. 4 (2023)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2023.032.04.1

Abstract

Inflammation is a physiological response to various stimuli including infection and tissue injury. One cause of inflammation is gram-negative bacteria that release various toxins, e.g. lipopolysaccharide endotoxin (LPS). The first body defense response is neutrophils. Body defense response is expressed by the cyclooxygenase (COX) enzyme that functions to transform arachidonic acid into prostaglandin. Polyphenols extract from Robusta coffee bean is known to play an anti-inflammatory role, but the mechanism for inhibiting COX-2 remains unknown. This study aimed to determine the potential of polyphenols extracted from Robusta coffee beans on COX-2 inhibition in neutrophils exposed to E. coli LPS. The research design was experimental in vitro with a post-test-only control group design. The sample consisted of 6 treatment groups, neutrophil isolates was incubated in concentrations of Robusta coffee polyphenols extract of 3.13%, 6.25%, 12.5%, and 25% and exposed to E. coli LPS. Measurement of COX-2 expression was conducted using immunohistochemical methods. ANOVA and LSD test results showed COX-2 expression in the treatment groups, namely neutrophils which were incubated with Robusta coffee polyphenols extract and exposed to E. coli LPS, was lower than the neutrophil group exposed only to E. coli LPS. The conclusion of this study is that the polyphenols extract of the Robusta coffee bean can inhibit COX-2 expression on neutrophils exposed to E. coli LPS.
Efek Ekstrak Buah Delima Merah terhadap Jumlah Fibroblas di Ligamen Periodontal Daerah Tarikan Gigi Tikus Wistar Selama Pergerakan Gigi Ortodonti Dina Nuur Rosyiidah; Happy Harmono; Herniyati Herniyati
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 20 No 2 (2023)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v20i2.44018

Abstract

Orthodontic useful for repairing the arrangement of teeth by remodeling the alveolar bone and periodontal ligament and then fibroblast proliferation will occur. Fibroblast play a role in the formation of collagen fibers that will connect teeth to bones. Orthodontic takes a long time so it takes a method to speed it up. One of them can use red pomegranate extract which contains antioxidants. This Research purpose to analyze the effect and impact of the duration of administration of red pomegranate extract on the number of fibroblasts in the 1st, 2nd, and 3rd weeks of the periodontal ligament in the tooth-tensioning area of wistar rats during orthodontic tooth movement. 36 rats divided into 6 groups: the group with orthodontic appliance installation without red pomegranate extract for 1 week (A), 2 weeks (B), and 3 weeks (C), and the orthodontic appliance installation group with red pomegranate extract administration for 1 week. weeks (D), 2 weeks (E) and 3 weeks (F). The provision of orthodontic mechanical forces by placing a ligature on the right M1 tooth and both maxillary incisors. Then the M1 is moved mesial using a tension gauge to produce a force of 11.5 gF with Ni-Ti closed coil spring. When the rats were euthanized and histology was made with Haematoxylin Eosin (HE) staining. Fibroblasts were observed by using microscope with 400 magnification in 3 fields of view. The average number of fibroblasts in all groups (A, B, C, D, E and F) increased and in the LSD test it was found that between groups there was a significant difference because it had a p-value ≤ 0.05 Red pomegranate extract increase the number of fibroblasts in the periodontal ligament tension area and there is an increase in the number of fibroblasts in the 1st week to the 3rd week.
Efek Ekstrak Ampas Seduhan Bubuk Kopi Dalam Menghambat Produksi Malondialdehid Pada Isolat Monosit Perifer Manusia yang Distimuli Dengan Eksotoksin Streptococcus mutans Fawwazi, Rafif Hanif; Harmono, Happy; Susilawati, I Dewa Ayu
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 21 No 1 (2024)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v21i1.47332

Abstract

Monocytes as non-specific immune cells provide an early response to pathogens and foreign objects that enter the body. When exposed to pathogens or foreign objects, monocytes experience an increase in the number of free radicals. Free radicals activate phospholipase enzymes that break down membrane phospholipids into phosphate, glycerol and fatty acids. Unsaturated fatty acids (PUFAs) react with free radicals to undergo peroxidation to produce malondialdehyde (MDA). It takes antioxidant compounds that can reduce excess free radicals in the body. Coffee grounds as solid waste from the consumption of processed coffee products are not used and disposed of, it is suspected that they can be used as antioxidants. This study aims to determine the effect of coffee grounds steeping antioxidants on the profile of MDA levels in monocytes stimulated by the S. mutans exotoxin (In Vitro). Research was done in experimental post test only control group design. The object of this study was human peripheral blood monocytes, which were isolated by the Gradient Density method. Following in vitro exposure to the exotoxin S. mutans, the monocyte isolates were subjected to treatment with brewed coffee grounds extract at three different concentrations (0.25 mg/ml, 0.50 mg/ml, and 0.75 mg/ml). The MDA levels were subsequently determined by employing the TBArs method on a UV-Vis spectrophotometer. The results of MDA levels were analyzed using One Way Anova and LSD. The MDA level data showed significance (p<0.05) with the average MDA level in the Negative Control group1.49 g/ml; Positive Control 10.62 g/ml; T1 8.15 g/ml; T2 6.59 g/ml; and T3 4.69 g/ml. It can be concluded that the extract of brewed coffee grounds can reducing MDA levels in monocytes exposed to the exotoxin of S. mutans.
STATUS KEBERSIHAN RONGGA MULUT DAN KEBUTUHAN PERAWATAN JARINGAN PERIODONTAL PADA LANSIA DI DAERAH PESISIR PUGER, KABUPATEN JEMBER Utha, Maria Fransisca; Fany, Manta; Wahyukundari, Melok Aris; Pudjiastuti, Peni; Prasetya, Rendra C.; Harmono, Happy; Wanodyo, Ari Tri; Sari, Desi Sandra
Jurnal Kesehatan Vol 17 No 2 (2024): JURNAL KESEHATAN
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/kesehatan.v17i2.38943

Abstract

Background: Periodontal disease is a dental and oral health problem that is often experienced by the elderly. Aging affects the ability of the elderly to carry out their daily activities, including cleaning the oral cavity. Increased accumulation of calculus due to poor oral hygiene can lead to the incidence of periodontal disease which affects the quality of life of the elderly. Purpose: to examine the status of oral hygiene and the need for periodontal tissue care in the elderly in the Pesisir Puger and Payangan area, Jember Regency. Methods: Descriptive observational with a cross-sectional approach. Subjects were selected as many as 192 elderly by purposive sampling technique. Data were obtained by taking questionnaires and clinical examinations in the form of OHI-S and CPITN. Results: the majority of elderly in the Pesisir Puger area of Jember district have a poor oral hygiene status of 96.88% and , the condition of the periodontal tissue has a 4-5 mm periodontal pocket of 66.67% and the need for periodontal tissue treatment is in the form of oral hygiene instructions and SRP procedures of 96.88%. Conclusion: Majority of the elderly in Pesisir Puger and Payangan have poor oral hygiene and require periodontal tissue treatment in the form of oral hygiene instructions and scaling and root planing procedures
Antibacterial Activity of Robusta Instant Coffee Powder Against Acidogenic and Aciduric Species of Streptococcus mutans Bacteria Lestari, Pujiana Endah; Susilawati, I Dewa Ayu; Harmono, Happy; Dewanti, I Dewa Ayu Ratna; Masari, Al; Nailufar, Khanun; Morauli, Fatricia
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.50812

Abstract

Streptococcus mutans is a major species that causes dental caries due to its acidogenic and aciduric properties. Coffee beans contain several compounds, such as caffeine, phenols, tannins, trigonelline, chlorogenic acid, and caffeic acid, which are known for their antibacterial properties. Robusta instant coffee powder is a stable product that is not easily contaminated with bacteria, fungi, and yeast. It is a zero-waste and soluble material. This study aimed to analyze the antibacterial activity of robusta instant coffee powder (RICP) against S. mutans. The antibacterial activity of RICP against S. mutans was tested using three antimicrobial susceptibility tests: 1) disk diffusion method, 2) minimum inhibitory concentrations (MIC), and 3) minimum bactericidal concentration (MBC). The results of the disk diffusion method test showed that the RICP concentration of 0.2 g/mL to 0.8 g/mL against S. mutans had an inhibition zone of 31.14 mm to 42.01 mm. MIC and MBC values of RICP against S. mutans were both 0.0313 g/mL. In conclusion, robusta instant coffee powder (RICP) demonstrates antibacterial activity against the acidogenic and aciduric S. mutans bacteria.
Evaluation of rat model oral candidiasis mucosal response immune Sulistyani, Erna; Triwahyuni, Iin Eliana; Harmono, Happy; Sholehah, Almaratus; Prameswari, Syefira Putri; Mayangsari, Zahra; Perwitasuci, Brigitta Damai; Wulandari, Widya Nurfitriastuti
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.172-179

Abstract

Background: The choice of antifungal drugs is limited and has not yet made significant progress. The animal model of oral candidiasis is a crucial factor in exploring antifungals. In our previous study's oral candidiasis rat model, we found an increase in the sedimentation rate and a decrease in infection microbiologically and histologically on day 8 compared to day five after inoculation. On the other hand, blood plasma levels of IL-17, as the main cytokine in fungal infections, keep at a low level. The study aims to prove the increase in the immune response in the oral mucosa of an oral candidiasis rat model on the 8th day after inoculation of C. albicans. Methods: The sample consisted of 2 groups; healthy Wistar rats as a control and Wistar Rat rats treated as an oral candidiasis rat model. Eight days after inoculating C. albicans,The immune response was evaluated by counting the number of visual field inflammatory cells on the dorsum of the rat dorsum tongue.Results: The number of inflammatory cells, including macrophages in the dorsal tongue mucosa of oral candidiasis rats, was higher than that of healthy rats. Conclusion: There was an increase in the number of inflammatory cells in the dorsum of the tongue mucosa of the oral candidiasis rat model on day 8, which is a sign of an increased immune response in the experimental animal model.
Toothbrush design appropriate for pre-elderly: The relationship of toothbrush bristles number to oral hygiene Suhartini, Suhartini; Hamzah, Zahreni; Setyaningsih, Sari; Prijatmoko, Dwi; Harmono, Happy; Arina, Yuliana Mahdiyah Daat; Prihatiningrum, Berlian
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.%p

Abstract

Background: The increase in the elderly population leads to a decline in their health and affects the productivity and quality of life. Preventive measures must be taken to improve oral hygiene for the elderly starting from the pre-elderly group so that later it will form healthy and productive elderly. One of the efforts to improve dental hygiene and health is through mechanical tooth cleaning with a toothbrush. However, currently, there is no toothbrush design that is suitable for the condition of the pre-elderly oral cavity. Therefore, it is necessary to analyze the design of the number of toothbrush bristles as initial research to obtain an appropriate toothbrush design for the elderly. Method:The study was conducted on 30 participants using a toothbrush with a number of toothbrush bristles paths: with 4 paths, 3 paths, and 2 paths. The effectiveness of tooth brushing hygiene was measured through the debris index before using a toothbrush and after brushing. Result: The results of the Kruskal Wallis test showed a significance value of 0.01 (p
Co-Authors Ainunnusak Ayuningtyas Anisa Nur Hakima Arina, Yuliana Mahdiyah Daat Cicik Khilar Rizqi Dania Kartikasari Desi Sandra Sari Didin Erma Indahyani Dina Nuur Rosyiidah Dwi Prijatmoko Erna Sulistyani Fany, Manta Fawwazi, Rafif Hanif Hakima, Anisa Nur Herniyati Herniyati Herniyati Hestieyonini Hadnyanawati I Dewa Ayu Ratna Dewanti I Dewa Ayu Susilawati IDA Susilawati Iin Eliana Triwahyuni Leliana Sabdra deviade Putri Leliana Sandra Devi Ade Putri Lisa Miftakhul Janna Maria Devitha Masari, Al Mayangsari, Zahra Melok Aris Wahyukundari Melok Aris Wahyukundari, Melok Aris Morauli, Fatricia Muchamad Ziyad Afif Nahdiya Fitriyana Nailah Rahmadani Nailufar, Khanun Perwitasuci, Brigitta Damai Prameswari, Syefira Putri Prasetya, Rendra C. Prihatiningrum, Berlian Pudjiastuti, Peni Pujiana Endah Lestari Qurratul Aini Wahyu Pratiwi Raditya Nugroho Riclas Yusuf Punta Wibisono Saikha Adila Azzah Sari Setyaningsih Setyaningsih, Sari Sholehah, Almaratus Sri Hernawati Suhartini Suhartini Tantin Ermawati Tantin Ermawati Utha, Maria Fransisca Wanodyo, Ari Tri Wulandari, Widya Nurfitriastuti Yuliana Mahdiyah Daat Arina Zahara Meilawaty Zahara Meilawaty Zahreni Hamzah