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PENGARUH PEMBERIAN PROBIOTIK SEBAGAI ADITIF PAKAN TERHADAP KADAR KOLESTEROL, HIGH DENSITY LIPOPROTEIN (HDL) DAN LOW DENSITY LIPOPROTEIN (LDL) DALAM DARAH AYAM KAMPUNG Suryo, Heinrich; Yudiarti, Turrini; Isroli, Isroli
Animal Agriculture Journal Vol 1, No 2 (2012): Volume 1, Nomor 2, Tahun 2012
Publisher : Animal Agriculture Journal

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Abstract

Probiotik adalah suplemen pakan mikrobia yang bermanfaat bagi ternak untuk menjaga keseimbangan mikrobia di dalam usus. Pemberian probiotik dapat menjaga keseimbangan komposisi mikrobia dalam saluran pencernaan ternak sehingga meningkatkan daya cerna bahan pakan dan menjaga kesehatan ternak. Tujuan penelitian ini adalah mengukur kadar kolesterol, HDL dan LDL darah ayam kampung yang diberi aditif pakan probiotik. Penelitian ini menggunakan Day Old Chick (DOC) ayam kampung sebanyak 200 ekor dengan bobot badan awal rata-rata 33,58 ± 0,8 gram, antikoagulen, EDTA, Vacuntainer, Centrifuge. Probiotik yang digunakan yaitu jenis kapang dan pakan gasal memiliki kandungan energi termetabolit 2750,000 kkal, protein kasar 20,900 %, lemak 6,260 %. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dengan 5 ulangan, masing-masing unit percobaan 20 ekor. Perlakuan yang diterapkan pada penelitian ini adalah tanpa penambahan dosis probiotik (T0), dengan tambahan dosis probiotik 0,25 g probiotik / 100 g pakan (T1), 0,50 g probiotik / 100 g pakan (T2), 0,75 g probiotik / 100 g pakan (T3). Hasil penelitian menunjukkan bahwa penambahan probiotik dalam pakan ayam kampung berpengaruh nyata (P<0,05) terhadap kadar HDL, LDL, dan Kolesterol. Rerata kadar HDL masing-masing perlakuan T0, T1, T2, dan T3 berturut-turut adalah 41,440; 45,920; 48,440 dan 50,400 mg/dl. Rerata kadar LDL masing-masing perlakuan T0, T1, T2 dan T3 berturut-turut adalah 78,089; 70,550; 45,677; dan 47,011 mg/dl. Rerata kadar Kolesterol masing-masing perlakuan T0, T1, T2 dan T3 berturut-turut adalah 163,529; 160,000; 132,941; dan 131,764 mg/dl. Simpulan penelitian adalah pemberian probiotik mampu meningkatkan kadar HDL, menurunkan kadar LDL dan Kolesterol darah ayam kampung. Hasil yang paling baik pada perlakuan taraf 0,75 g/100 g (T3).Kata kunci : Probiotik, ayam kampung, kolesterol, HDL, LDL.ABSTRACT Probiotic is a supllement feed mikrobia which beneficial for cattle to keep mikrobia balance in gut. Giving probiotic can keep the composition mikrobia in digestive tract of cattle to increase the power of gastrointestinal tract and keep healty of cattle. The aims of research was to measure the levels of cholesterol, HDL and LDL in blood of indigenous chicken with given as probiotics feed additive. This study used Day Old Chick (DOC) of 200 chicken with initial body weight of an average of 33.58 ± 0.8 grams, antikoagulen, EDTA, Vacuntainer, Centrifuge. Probiotics used which are the kind of mold. The composition of basal feed are metabolizable energy (ME) 2750 kcal, 20.9% crude protein, 6.260% fat. Experimental design used was completely randomized design (CRD) consisting of 4 treatments with 5 replicates, each experimental unit 20. The treatment was dose of probiotic T0 without probiotics addition, applied 0.25 g probiotic / 100 g (T1), 0.50 g probiotic / 100 (T2), 0.75 g probiotic / 100 g (T3). The results showed that addition of probiotics affected (P <0.05) to the levels of Cholesterol, HDL, LDL. The average HDL levels each treatment T0, T1, T2 and T3 were 41,440; 45,920; 48,440 and 50,440 mg/dl respectively. The average LDL levels each treatment T0, T1, T2 and T3 were 78,089; 70,550; 45,677; and 47,011 mg/dl respectively. The average Cholesterol levels each treatment T0, T1, T2 and T3 were 163,529; 160,000; 132,941; and 131,764 mg/dl respectively. The emelutions the addition of probiotics increased levels of HDL, decreased levels of LDL and cholesterol in indigenous chickens.Key words: probiotics, indigenous chicken, Cholesterol, HDL, LDL
PENGARUH PEMBERIAN EKSTRAK KUNYIT (Curcuma domestica) TERHADAP KADAR AIR, PROTEIN DAN LEMAK DAGING AYAM BROILER Estancia, Kafaah; Isroli, Isroli; Nurwantoro, Nurwantoro
Animal Agriculture Journal Vol 1, No 2 (2012): Volume 1, Nomor 2, Tahun 2012
Publisher : Animal Agriculture Journal

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak kunyit terhadap kadar air, protein dan lemak pada daging ayam broiler. Penelitian dilakukan dari bulan November - Desember 2011 di Fakultas Peternakan, Laboratorium Teknologi Hasil Ternak Fakultas Peternakan dan Pertanian Universitas Diponegoro. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) terdiri dari 5 perlakuan yaitu tanpa ekstrak kunyit (T0), pemberian ekstrak kunyit 100mg/kgBB/hari (T1), pemberian ekstrak kunyit 200 mg/kgBB/hari (T2), pemberian ekstrak kunyit 300mg/kgBB/hari dan pemberian ekstrak kunyit 400 mg/kgBB/hari, masing-masing 4 ulangan dan setiap unit terdiri dari 3 ekor. Parameter yang diamati dalam penelitian ini adalah kadar air, protein dan lemak daging ayam broiler. Hasil penelitian didapatkan untuk perlakuan T0, T1, T2, T3, dan T4 berturut-turut rata-rata kadar air 74,75 %; 75,48 %; 75,98 %; 74,98 % dan 75,38 %. Persentase rata-rata kadar protein 19,31 %; 18,7 %, 19,4 %, 19,91 % and 19,89 %. Presentase rata-rata kadar lemak adalah 0,89 %; 0,81 %; 1,96 %; 0,88 %; 1,28 %. Pemberian ekstrak kunyit (Curcuma domestica) tidak berpengaruh terhadap kadar air, protein dan lemak daging ayam broiler.Kata Kunci : ekstrak kunyit, daging ayam broiler, kadar air, protein, lemakABSTRACTThis study aims to determine the effect of turmeric extract addition on the water, protein and fat levels of broiler chicken meat. The study was conducted from the month of November - December 2011 Faculty of Animal Husbandry and Agriculture, Laboratory of Livestock Technology Faculty of Animal Husbandry in the and Agriculture University of Diponegoro. The design of the study is a completely randomized design (CRD) consisting of 5 treatments that is without turmeric extract (T0), 100 mg/kg (T1), 200 mg/kg (T2), 300 mg/kg (T3) and 400 mg/kg (T4) body weight per day, each one 4 replications and consist 3 chickens. The parameters observed were water, protein and fat levels. Treatment T0, T1, T2, T3, and T4 each having average for water level 74.75 %, 75.48 %, 75.98 %, 74.98 % and 75,38 %. Percentage average for protein level 19.31, 18.7 %, 19.4 %, 19,91 and 19.89 %. Percentage average for fat level 0.89 %, 0.81 %, 0.96 %, 0.88 % and 1.28 %. Extract of turmeric (Curcuma domestica) no effect on level water, protein and fat meat of broiler chicken.Key words : turmeric extract, broiler chickens meat, water, protein, fat
PENGARUH AIR REBUSAN KUNYIT (CURCUMA DOMESTICA) DALAM AIR MINUM TERHADAP DAYA TAHAN DAN PERFROMANS KARKAS AYAM BROILER Ma?rifah, Binti; Isroli, Isroli; Sartono, Tri Agus
JURNAL RISET AGRIBISNIS & PETERNAKAN Vol 5, No 1 (2020): JURNAL RISET Agribisnis & Peternakan
Publisher : JURNAL RISET AGRIBISNIS & PETERNAKAN

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh air kunyit rebus terhadap daya tahan dan produksi karkas ayam broiler. Penelitian menggunakan 200 ekor DOC (day old chicken) ayam broiler dengan berat awal 41,48 ± 0,99 g/ekor. Pakan yang digunakan adalah pakan komersial dengan kode B-11S untuk fase starter dan BR-1AJ untuk fase finisher. Desain yang digunakan dalam penelitian ini adalah rancangan acak lengkap (RAL) dengan 5 perlakuan dan 5 ulangan yang masing masing terdiri dari 8 ekor. Perlakuan terdiri dari T0 (100% air), T1 (75% air + 25% air rebusan kunyit), T2 (50% air + 50% air rebusan kunyit), T3 (25% air + 75% air rebusan kunyit), T4 (100% air rebusan kunyit). Hasil penelitian menunjukkan bahwa air kunyit rebus memberikan pengaruh yang signifikan (P&#60;0,05) terhadap rasio jumlah heterofil : limfosit dan porsi potongan karkas bagian dada, namun tidak berpengaruh terhadap jumlah sel limfosit, dan neutrophil serta bobot badan akhir, bobot karkas dan potongan karkas (sayap, femur, betis dan punggung). Kesimpulan dari penelitian ini adalah bahwa penggunaan air kunyit rebus dalam air minum ayam pedaging mampu memperbaiki derajat kesehatan ayam dan meningkatkan performans potongan karkas ayam broiler.
Effect of decocted turmeric on performance, hematological parameters and carcass traits of broiler chickens Isroli, I.; Yudiarti, T.; Widiastuti, E.; Sugiharto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 4 (2017): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.4.263-269

Abstract

This study aimed to investigate the effect of decocted turmeric on performance, haematological parameters and carcass traits of broilers. Broiler chicks at 11 days of age were allotted to five groups, including T0 (birds drinking 100% water), T1 (25% decocted turmeric + 75% water), T2 (50% decocted turmeric + 50% water), T3 (75% decocted turmeric + 25% water) and T4 (100% decocted turmeric). The study was arranged based on a completely randomized design (CRD), parameters investigated was performance and blood chemistry (leukocyte, total protein, albumin, globulin, uric acid, glucose). The data were subjected to analysis of variance at 5% probability. Decocted turmeric did not affect weight gain, feed and water consumption and feed conversion ratio of broilers. Lymphocytes proportion was higher (P<0.05) in T1, T2 and T4 than in T0 and T3 birds. The heterophils to lymphocytes ratio was lower (P<0.05) in birds receiving decocted turmeric than control. Globulin concentration and albumin to globulin ratio were higher and lower (P<0.05), respectively, in serum of T2, T3 and T4 than T0 and T1 birds. The proportion of heterophils and concentration of serum total protein, albumin, uric acid and glucose were not different among treatments. The weight of breast meat was higher and lower (P<0.05) in T3 and T4 birds, respectively, compared to the control. In conclusion, providing decocted turmeric through drinking water helped to improve stress responses and increased the weight of breast meat of broiler chickens. 
MUSCLE FIBER DIAMETER AND FAT TISSUE SCORE IN QUAIL (Coturnix-coturnix japonica L) MEAT AS AFFECTED BY DIETARY TURMERIC (Curcuma longa) POWDER AND SWANGI FISH (Priacanthus tayenus) MEAL Kartikayudha, W.; Isroli, I.; Suprapti, N.H.; Saraswati, T.R.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 4 (2013): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.4.264-272

Abstract

The objectives of this study were evaluate the dietary turmeric powder and swangi fish meal onsize of muscle fiber diameter and fat tissue score of major pectorales and semimembranosus of quailmeat. Research was conducted based on 2x3 of factorial completely randomized design, in which thefirst factor was 2 types of diet, i.e. RA : standard diet; RB : 85% standard diet + 15% swangi fish meal,and the second factor was 3 levels of period time of turmeric powder addition, i.e. P0 : without turmericpowder; P1 : turmeric powder 54 mg/quail/day was given since quail age 210 days old; P2 : turmericpowder 54 mg/quail/day was given since quail age 14 days old. Difference of means between treatmentgroups were analyzed by Duncan’s Multiple Range Test in 95% significance level. The results showedthat combined treatment of tumeric powder with RA (P0 : 2.33 μm; P1 : 3.06 μm; P2 : 2.98 μm) and RB(P0 : 2.22 μm; P1 : 3.12 μm; P2 : 2.92 μm) increased (P<0.05) muscle fiber diameter on majorpectorales significantly. Muscle fiber diameter on semimembranosus were increased (P<0.05) bycombined treatment of tumeric powder with RA (P0 : 2.83 μm; P1 : 3.50 μm; P2 : 3.24 μm) and RB (P0= 2.85 μm; P1 = 3.28 μm; P2 = 3.33 μm). In conclusion, combined treatment of RA (standard diet) andRB (diet with Swangi fish meal) increased (P<0.05) the size of muscle fiber diameter in majorpectorales and semimembranosus.
Effect of dietary supplementation of formic acid, butyric acid or their combination on carcass and meat characteristics of broiler chickens Sugiharto, S.; Yudiarti, T.; Isroli, I.; Widiastuti, E.; Wahyuni, H. I.; Sartono, T. A.; Nurwantoro, N.; Al-Baarri, A. N.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.3.286-294

Abstract

The objective of the present study was to evaluate the impact of dietary supplementation of formic acid, butyric acid or their combination on carcass and meat characteristics of broiler chicks. A number of 288 day-old-chicks (Lohmann MB-202 meat broilers) were distributed to four groups, including CONT (basal diet with no additive as a control), FORM (basal diet containing 0.1% formic acid), BUTR (basal diet with 0.03% butyric acid) and FORM+BUTR (basal diet with 0.1% formic and 0.03% butyric acid). At day 35 the birds were slaughtered and eviscerated, and from which the breast meat was obtained. Carcass and cut yields of broilers were not different (P>0.05) among CONT, FORM and BUTR birds. The combination of formic and butyric acids increased (P<0.05) the relative weight of back of broilers. Meat pH value was higher (P<0.05) in organic acids treated than in non-treated birds. Organic acids lowered (P<0.05) drip loss of broiler meat. Butyric acid increased (P<0.05) moisture and decreased (P<0.05) the content of meat protein. Formic acid increased (P<0.05), whereas butyric acid and combination of formic and butyric acids decreased (P<0.05) lightness values of breast meat. Yellowness values of meat increased (P<0.05) with organic acid administration. Intense bands of 25 kDa (phosphoglycerate mutase) was observed in most of meat samples from the treated birds, but not in meat from the control birds. In conclusion, dietary supplementation of organic acids was capable of improving the meat quality of broiler meats. The treatments may prevent the pale, soft and exudative (PSE)-like condition in broiler meats. 
Breast meat characteristics of broilers fed fermented mixture of cassava pulp and Moringa oleifera leaf meal Sugiharto, S.; Widiastuti, E.; Isroli, I.; Yudiarti, T.; Sartono, T. A.; Wahyuni, H. I.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 2 (2020): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.2.103-114

Abstract

The present study aimed to investigate the effect of feeding fermented mixture of cassava pulp and M. oleifera leaf meal (FCPMO) on the breast meat characteristics of broiler chickens. From day 8 onward, 400 Lohmann broiler chicks were allotted to four dietary groups included CONT (maize-soybean-based feed with no additive), BACI (maize-soybean-based feed supplemented with 0.1% zinc bacitracin), FERM (feed containing 20% of FCPMO) and FERB (feed containing 20% of FCPMO and 0.1% probiotic Bacillus subtilis). At day 35, one male chick from each replicate (10 chicks per treatment group) was taken and slaughtered. After being eviscerated and de-feathered, sample of breast meat was obtained for the assessment of meat characteristics. Results showed that feeding FCPMO had no effect (P>0.05) on pH and water holding capacity (WHC) of breast meats. Compared to other groups, FERB meat had higher (P<0.05) cooking loss. FERM and FERB meats had lower (P<0.05) moisture content than that of CONT and BACI meats. Feeding FCPMO with or without B. subtilis increased (P<0.05) crude protein content of breast meats. Feeding FCPMO resulted in lower (P<0.05) fat content in the breast meat. Compared to other meats, breast meats from BACI had higher (P<0.05) ash content. Cholesterol was lower (P=0.08) in FERB than in BACI and FERM meats. The 2,2-diphenylpicrylhydrazyl (DPPH) IC50 values were higher (P<0.05) in meat of CONT than that in other groups. Feeding FCPMO resulted in higher (P<0.05) polyunsaturated fatty acids (PUFA), lower (P<0.05) monounsaturated fatty acids (MUFA) and had no effect on saturated fatty acids (SFA) contents of broiler breast meats. In conclusion, feeding FCPMO produced healthy broiler meat as reflected by the higher contents of protein, PUFA and antioxidants, and lower fat in meats.
PHYSIOLOGICAL RESPONSES OF BLOOD AND IMMUNE ORGANS OF BROILER CHICKEN FED DIETARY BLACK CUMIN POWDER (Nigella sativa) DURING DRY SEASONS Salam, S.; Sunarti, D.; Isroli, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 3 (2013): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.3.185-191

Abstract

This study was designed to determine the physiological response of blood and immune organs ofbroiler chickens fed on various concentration of dietary black cumin powder (BCP) during the dryseason. A total number of 100 unsexed one-day old Cobb broiler chicks were used and distributed to 5treatments (control, antibiotics and without BCP, 20 g/kg BCP, 40 g/kg BCP and 60 g/kg BCP) and 4replications (5 birds for each). Physiological responses of blood and immune organs were measured at30 day of age. Addition of BCP to broiler ration did not significantly effects on physical properties ofblood (leukocytes count, erythrocytes count, haemoglobin, hematocrit, monocytes, and eosinophils) andrelative weights of thymus and bursa of fabricius, but significantly (P<0.05) increased relative weightsof spleen when compared to control. It was concluded that the black cumin grinds (Nigella sativa) as afeed additive could not change the physical properties of blood, relative weights of thymus and bursa offabricius, but it increased the relative weight of spleen at the level of 60 g/kg BCP, which could reduceadverse effects of infectious diseases in broiler chicken.
EFFECT OF POST-HATCH FEED DEPRIVATION ON FATTY ACID COMPOSITION OF BROILER MEAT Sugiharto, S.; Isroli, I.; Yudiarti, T.; Widiastuti, E.; Kusumanti, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 4 (2015): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.4.234-237

Abstract

The objective of the present study was to investigate the effect of feed deprivation over the first 48 h post-hatch on the composition of fatty acids in broiler meat. Sixty 1-d-old Ross chicks were divided to two experimental groups, i.e., birds provided access to feed and water ad libitumimmediately post-hatch until d 35 and birds deprived from feed but not from water over the first 48 h post-hatch. Blood for cholesterol analysis was collected on d 34. The same birds were sacrificed on d 36, and immediately breast meat, abdominal fat, heart and liver were collected and weighed.Feed deprivation over the first 48 h post-hatch retarded (P<0.05) growth performance of chickens, but had no impact (P>0.05) on the abdominal fat, fatty acid profile of meat, total serum cholesterol level as well as the weight of heart and liver of broiler. In conclusion, early post-hatch feeding is a beneficial means to enhance the final body weight of broilers without affecting the meat quality.
EFFECT OF TURMERIC EXTRACT ON BLOOD PARAMETERS, FEED EFFICIENCY AND ABDOMINAL FAT CONTENT IN BROILERS Sugiharto, S.; Isroli, I.; Widiastuti, E.; Prabowo, N.S.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 1 (2011): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.1.21-26

Abstract

The experiment was carried out to investigate the effect of turmeric extract on blood parameters,feed efficiency and abdominal fat content in broilers. A total of 100 mixed-sexes Lohman chicks wereused in the experiment set up with completely randomized design of 5 different doses of turmeric extractgiven orally start from day-15 to day-35. The chicks given no turmeric extract were used as a control(T0), whereas T1, T2, T3 and T4 were the chicks given turmeric extract of 200, 400, 600 and 800mg/kg-live BW, respectively. Hemoglobin values of broilers given turmeric extract 800 mg/kg-live BWwas significantly higher (p<0.05) at week-3 and week-4, but not at week-5, compared to broilers giventurmeric extract with lower doses or control group. Turmeric extract did not affect significantly (p>0.05)the concentration of erythrocytes, the levels of hematocrit as well as FCR and abdominal fat content ofbroiler. Turmeric extract seemed to be able to improve the digestion and metabolism (of fat) resulted inenhanced production of hemoglobin in broilers. The hypolipidemic effect of turmeric that could hamperits potential to promote the growth of broilers per se might explain why turmeric could not improve thefeed efficiency of broiler.