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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Hemera Zoa Media Veteriner Jurnal Veteriner JOURNAL OF COASTAL DEVELOPMENT Jurnal Kedokteran Hewan
Ita Djuwita
Departemen Anatomi Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, IPB, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : HAYATI Journal of Biosciences

 1 
Development of Domestic Cat Embryo Produced by Preserved Sperms KARTINI ERIANI; ARIEF BOEDIONO; ITA DJUWITA; SONY HERU SUMARSONO; AL-AZHAR AL-AZHAR
HAYATI Journal of Biosciences Vol. 15 No. 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.643 KB) | DOI: 10.4308/hjb.15.4.155

Abstract

The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether both epididymis sperms and oocytes can be used to produce viable embryos and offspring. The purpose of this study was to determine how long cats sperms contained in epididymis were remain motile and had intact membranes when preserved at 4 oC, and to determine whether such those preserved sperms are able to fertilize oocytes. Epididymis was preserved immediately in phosphate buffer saline at 4 oC for 1, 3, and 6 days. The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. The results showed that average motility of sperms collected from ductus deferens, cauda and corpus epididymis decreased not significantly (P > 0.05) from 0, 1, 3, and 6 days of preservation times (from 83.0%, 80.2%, 79.0%; 80.9%, 75.0%, 75.5%; 52.0%, 63.2%, 55.0% to 34.6%, 34.6%, 33.3%, respectively). The general results showed that sperms from epididymis preserved for 1, 3, and 6 days can be used for IVF. The rate of embryonal cleavage produced by IVF technique using sperms collected from epididymis preserved for 1-, 3- and 6-days were 33.3, 26.7, and 20.0%, respectively and significantly different (P < 0.05) from that of controll (50.0%). In conclusion, sperms contained in epididyimis preserved at 4 oC in PBS (Phospate Buffer Saline) for 1-6 days can be used to IVF and in vitro production of cat embryos. Key words: gamet, preservation, in vitro fertilization
In Vitro Fertilization and Embryo Development ITA DJUWITA; ARIEF BOEDIONO; SRIHADI AGUNGPRIYONO; IMAN SUPRIATNA
HAYATI Journal of Biosciences Vol. 12 No. 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.772 KB) | DOI: 10.4308/hjb.12.2.73

Abstract

Experiments were conducted on the morphology, fertilization and embryo development rate of vitrified ovine oocytes matured in vitro. Three vitrification solutions were used for vitrification. PBS supplemented with 1% BSA, 30% ethylene glycol was added by one of three different sucrose concentrations, 1.00 M (VS1), 0.50 M (VS2), and 0.25 M (VS3). The results showed that the percentages of normal vitrified oocytes after warming were 78 and 63% in VS1 and VS2, respectively, which was significantly higher as compared for VS3. The fertilization rates were 59 and 66% in VS1 and VS2, respectively, which were also significantly higher as compared with VS3 (35%). Zygote viability after 18 h was 57; 43; and 40%, for VS1,VS2, and VS3, respectively, which was not significantly different. The incidence of polyspermic penetration increased with increasing sucrose concentration, i.e 23, 11, and 9% in VS1, VS2, and VS3, respectively, as compared with unvitrified oocytes (4%). The cleavage rate of vitrified oocytes in VS1 was 13.2% which was significantly lower (p
Co-Authors Adi Winarto AL-AZHAR AL-AZHAR Ari Purbayanto Arief Boediono Bambang Purwantara Bibiana W Lay BIBIANA W LAY Chairun Nisa Clara M. Kusharto Deny Putra Romadhon Erdiansyah Rahmi Etty Riani Faralinda Sari Fitri Susana Hamny Sofyan Harald Asmus Heru Setijanto I Ketut Suatha I Wayan Batan Iman Supriatna KARTINI ERIANI Katrin Roosita Ketut Adnyane Mudite Koeswinarning Sigit Kusdiantoro Mohamad M Agus Setiadi Mohamad Fakhrudin Mokhamad Fahrudin Muhamad Rizal Martua Damanik N adiarti Nining Handayani Nining Handhayani Nurhidayat - Rimbawan R Roza Helmita SONY HERU SUMARSONO Srihadi Agungpriyono Sugeng Budiharsono Supar - Supar . TAKDIR SAILI Tutik Wrediati Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esti Prasetyaningtyas Wahono Esti PrasetyoningtyaserB Wahyudin - Yohan Rusiyanto Yuhara Sukra