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All Journal Biology, Medicine, & Natural Product Chemistry BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Pendidikan Biologi Jurnal Biologi Tropis Jambura Journal of Animal Science Jambura Edu Biosfer Journal Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Journal of Natural Sciences Jambura Journal of Husbandry and Agriculture Community Serve Jurnal Pendidikan, Sains dan Teknologi Journal of Tropical Mycorrhiza HUMANIORASAINS Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Wirnangsih D. Uno
Universitas Negeri Gorontalo
Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Response of Actinomycetes Inoculation to the Growth Rate of waxy Corn (Zea mays var. ceratina) Adelia Salsabila Rahman; Novri Youla Kandowangko; Wirnangsih D. Uno; Yuliana Retnowati; Devi Bunga Pagalla; Hayatiningsih Gubali; Muhammad Isra; Aisyah Ahmad
Journal of Tropical Mycorrhiza Vol. 5 No. 1 (2026): April
Publisher : Asosiasi Mikoriza Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/jtm.v5i1.119

Abstract

Waxy corn (Zea mays var. ceratina) is an important food crop whose growth and productivity are strongly influenced by nutrient availability and soil microbial activity. The use of Actinomycetes as a biological biostimulant has gained attention due to its potential to enhance plant growth in an environmentally sustainable manner. This study aimed to evaluate the response of waxy corn growth rate to different doses of Actinomycetes inoculation. The experiment was conducted from July to December 2025 using a Randomized Block Design (RBD) consisting of three treatments: without Actinomycetes application (PA0), 5 g Actinomycetes (PA1), and 10 g Actinomycetes (PA2), with three replications for each treatment. Plant growth observations were performed at 14, 21, 28, 35, and 42 days after planting (DAP). Growth rate was determined based on the increase in plant dry biomass over each observation interval. The results showed that Actinomycetes inoculation affected the growth rate of waxy corn. The PA2 treatment consistently exhibited higher and more stable growth rates compared with PA0 and PA1 throughout the observation period. The enhanced growth performance was likely associated with the role of Actinomycetes in improving nutrient availability and stimulating vegetative development. In conclusion, the application of 10 g Actinomycetes demonstrated the greatest potential to increase the growth rate of waxy corn and may serve as an effective biological biostimulant for sustainable crop production.
Antagonistic Ability and Fungicide Tolerance of Actinomycetes from the Rhizosphere of Maize (Zea mays L.) Plants in Gorontalo Danial Mohamad; Yuliana Retnowati; Novri Youla Kandowangko; Abubakar Sidik Katili; Wirnangsi Din Uno
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1193-1201

Abstract

Actinomycetes are a group of microbes that live in the rhizosphere of plants and play an important role in soil microbial dynamics. This study aims to describe the antagonistic ability against pathogenic molds and tolerance to fungicides in actinomycetes from the rhizosphere of maize plants in Gorontalo. This research is descriptive uses a quantitative approach and employs a purposive sampling technique. Rhizosphere soil samples were collected from maize fields in the hilly area of Bontula Village, Asparaga District, Gorontalo Regency. The results showed the presence of 7 actinomycetes isolates with different morphological characteristics, namely RFZm-PG, RFZm-PLG, RFZm-PW, RFZm-PB, RFZm-PR, RFZm-PY, and RFZm-PO. Antagonistic tests showed that four isolates (RFZm-PB, RFZm-PG, RFZm-PLG, RFZm-PW) inhibited the growth of Fusarium oxysporum and Neocosmospora solani with an inhibition percentages of 66.38%-89%. The fungicide tolerance test showed that the seven isolates were tolerant to thiram, dimethomorph and benomyl, with the highest inhibitory effect by thiram. Isolates RFZm-PG and RFZm-PLG had the highest tolerance up to 500 ppm, while other isolates were tolerant only up to 400 ppm. Molecular analysis showed that RFZm-PG belongs to the genus Streptomyces (100% similarity with Streptomyces enissocaesilis), while RFZm-PLG belongs to Nocardiopsis (100% similarity with Nocardiopsis synnemataformans).
Pembuatan Cuka Ekstrak Jagung (Zea Mays) Dengan Menggunakan Acetobacter Aceti Dan Saccharomyces Cerevisiae Velma Agtavia; Lisnawati Botutihe; Moh Randy Putra Preatama Thalib; Ani M Hasan; Wirnangsi D Uno
Jurnal Pendidikan, Sains Dan Teknologi Vol. 4 No. 4 (2025): Oktober-Desember
Publisher : CV. ITTC INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47233/jpst.v4i4.3735

Abstract

This study aims to produce natural vinegar from corn extract (Zea mays L.) through a two-stage fermentation process: alcoholic fermentation using Saccharomyces cerevisiae followed by acetic acid fermentation using Acetobacter aceti. The evaluation included physical observation, pH measurement, lactic acid bacteria (LAB) testing, organoleptic assessment, and determination of acetic acid concentration through titration. The results indicated notable physical changes during fermentation, with pH decreasing from approximately 7 to 6 over 7–14 days, accompanied by the emergence of a distinctive vinegar aroma that signified acetic acid formation. LAB activity was confirmed by colony growth on Nutrient Agar (NA) media, demonstrating their role in establishing an acidic environment favorable for the second fermentation stage. Overall, the findings show that corn extract has strong potential as a fermentation substrate for natural vinegar production using a biological process.
Co-Authors Abubakar Sidik Katili Adelia Salsabila Rahman Aisyah Ahmad Alfandi Kibu Aryati Abdul Aryati Abdul Aryati Abdul Chairunnsah J. Lamangantjo Danial Mohamad Devi Bunga Pagalla Djuna Lamondo Djuna Lamondo Febriyanti Febriyanti Febriyanti HASAN, ANI M. Hayatiningsih Gubali Herinda Mardin Ilyas Husain Jusna Ahmad Kartika, Dika Kasim Ulane Laksmyn Kadir Lilan Dama Lisnawati Botutihe Magfirahtul Jannah Margaretha Solang Masra Latjompoh Moh Randy Putra Preatama Thalib Moha, Siti Nurliana Muh. Nur Akbar Muhammad Isra Mustamin Ibrahim Nazifah R Adam Nina Tanjung Api Novri Youla Kandowangko Nur'ain Marton Angio Nurdin Rachmi Subula Rhosfiani Hasan Safri Muhaimin Datau Sri Rahayu Thalib Syam S. Kumaji Velma Agtavia Wiwin Kobi Yuliana Nurdian Toini Yuliana Retnowati Yuniyarti Hulu Yusriyah Yusriyah