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Molecular Characteristics of Geoffroy's Rousette Rousettus amplexicaudatus Based on Cytochrome C Oxidase Subunit I and Cytochrome b Genes Kamilah, Santi Nurul; Jarulis, Jarulis; Sitompul, Aida Fitriani; Mardiah, Mifta; Ervinda, Mira; Khoirillah, Fanni; Lestari, Fitri Dwi; Zulkani, Dinda
BIOEDUSCIENCE Vol 9 No 1 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/15226

Abstract

Background: Rousettus amplexicaudatus is widely distributed across Indonesia, including the Suruman Cave in South Bengkulu. Due to similarities in morphology within the Rousettus group, identification can be challenging. We conducted a molecular analysis using COI and Cytochrome b genes from mitochondrial DNA to explore its genetic traits. DNA was extracted from the blood tissue of seven individuals from the Suruman Cave population, and gene amplification was performed with 20 bp primers. Sequence data were analyzed using MEGA XI software. Results: As a result, characteristics of the COI gene, which is 897 bp in length, were characterized by a high frequency of base pairs Adenine-Thymine (55.5%) and Guanine-Cytosine (44.5%), with the majority of the DNA sequence exhibiting a high degree of conservation sites (97.8%). The average intrapopulation genetic distance based on the COI gene was 0.77%, with four specific sites for R. amplexicaudatus Suruman Cave. The Cytochrome b gene, which is 635 bp long, is characterized by Adenine-Thymine base pairs of 53.7% and Guanine-Cytosine of 46.3%. Cytochrome b is more conserved than the COI (99.1%). The average intrapopulation genetic distance based on the Cytochrome b gene is 0.3% and has no population-specific sites. Conclusions: Both sequences showed a consistent pattern in phylogenetic tree analysis, which suggests the Suruman population is the group of R. amplexicaudatus. Therefore, these sequences can be proposed as molecular markers for R. amplexicaudatus, particularly when compared to the whole sequences of the COI and cytochrome b.
Aktivitas Harian Macaca fascicularis di Kawasan Kebun Campuran Sarti, Yili; Kamilah, Santi Nurul; Jarulis, Jarulis
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 4 No. 1 (2024): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/organisms.v4i1.22217

Abstract

Indonesia is the largest habitat for numerous species of primate, one of them is Macaca fascicularis. This species can live in various habitats, ranging from primary forests to residential areas. One of the habitats where M. fascicularis lives is a mixed garden area in Kepala Curup Village, Binduriang Sub-district, Rejang Lebong Bengkulu, which is adjacent to a residential area. The objective of this study was to observe the daily activities of a group of M. fascicularis in that area. Data were collected from April to June 2020. The sample objects were determined using the focal animal sampling method, which consisted of four individuals: alpha males, adult females, adolescents, and juveniles. Each individual was identified based on their physical characteristics. Data were collected using the continuous-time recording method, with a total observation of 16,800 minutes. The study revealed that M. fascicularis activity in the mixed garden area was highest in feeding (34.36%), followed by moving (26.43%), resting (25.36%), playing (7.20%), grooming (5.61%), vocalizing (0.55%), and conflict (0.50%). No mating activity was observed. The group size of the M. fascicularis in this area was limited (n = 9) and consisted of only one male. All adult females were in the period of lactation, which resulted in a low level of mating activity and conflict within the group AbstrakIndonesia merupakan habitat terbesar dari berbagai jenis primata, termasuk Macaca fascicularis. Primata ini dapat dijumpai pada berbagai tipe habitat, mulai dari hutan primer hingga Kawasan pemukiman penduduk. Salah satunya habitat ditemukannya M. fascicularis adalah di kebun campuran Desa Kepala Curup Kecamatan Binduriang Kabupaten Rejang Lebong yang berbatasan dengan pemukiman penduduk. Tujuan dari penelitian ini adalah untuk mengamati aktivitas harian pada satu kelompok M. fascicularis pada kawasan kebun campuran ini. Pengambilan data dilakukan pada bulan April hingga Juni 2020. Penentuan objek sampel menggunakan metode focal animal sampling, terdiri dari empat individu target yaitu jantan alfa, betina dewasa, remaja dan juvenile. Masing-masing individu telah dikenali berdasarkan ciri fisiknya. Data diambil menggunakan metode continuous time recording dengan total waktu pengamatan 16.800 menit. Dari penelitian yang telah dilakukan diketahui bahwa aktivitas M. fascicularis di kebun campuran tersebut tertinggi pada aktivitas makan (34,36%), kemudian diikuti aktivitas berpindah (26,43%), istirahat (25,36%), bermain (7,20%), grooming (5,61%), bersuara (0,55%), konflik (0,50%). Tidak ditemukan aktivitas kawin. Kelompok ini termasuk kelompok dengan anggota yang sangat sedikit (9 individu), dengan hanya terdapat 1 individu jantan. Semua betina dewasa dalam masa menyusui bayi. Kondisi itu berpengaruh pada rendahnya aktivitas kawin dan konflik di dalam kelompok. 
Analisis Pertumbuhan Fragmen Karang Heliopora sp. pada Substrat Tipe Web Spider di Perairan Pulau Tikus Pardede, Meri Kristina; Bakhtiar, Deddy; Jarulis, Jarulis; Sugara, Ayub; Nirawandi, Iwan; Margono, Setio
Buletin Oseanografi Marina Vol 14, No 3 (2025): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v14i3.69965

Abstract

Pulau Tikus adalah sebuah pulau kecil di Samudera Hindia yang berjarak sekitar 9 km dari Kota Bengkulu. Kondisi tutupan karang hidup yang rendah dan pemulihan kondisi terumbu karang sangat lambat, sehingga intervensi rehabilitasi melalui transplantasi karang sangat diperlukan untuk mengembalikan fungsi ekosistem terumbu karang yang telah rusak. Transplantasi karang yang digunakan adalah metode rak besi tipe spider web, cara ini dianggap sebagai salah satu teknik rehabilitasi karang yang paling berhasil. Salah satu jenis life form karang yang banyak ditemukan di Pulau Tikus adalah life form karang Heliopora, sehingga life form karang ini dijadikan objek untuk transplantasi karang. Tujuan dari penelitian ini adalah untuk menganalisis pertumbuhan dan kelangsungan hidup fragmen Heliopora yang ditransplantasikan pada substrat tipe spider web di perairan Pulau Tikus. Metode yang digunakan dalam penelitian ini adalah metode observasi dengan mengamati pertumbuhan fragmen karang pada 3 rangka web spider setiap 2 bulan selama 6 bulan serta menghitung jumlah fragmen karang yang mati selama pemeliharaan. Hasil penelitian, didapatkan bahwa nilai rata-rata pertumbuhan tinggi fragmen Heliopora selama 6 bulan pengamatan adalah 1,3 mm/bulan, sedangkan rata-rata pertumbuhan untuk lebar fragmen Heliopora adalah 1,75 mm/bulan. Transplantasi fragmen Heliopora pada rangka web spider di perairan Pulau Tikus, Kota Bengkulu dikatakan berhasil dengan tingkat kelangsungan hidup sekitar 50-75%. Tingkat kelangsungan hidup yang rendah diduga karena adanya aktivitas antropologi nelayan penangkapan ikan.
Variasi Pola Sidik Bibir Pada Siswa Penyandang Down Syndrome dari Beberapa Sekolah di Kota Bengkulu Kamilah, Santi Nurul; Aryanti, Fepta; Lestari, Dian Fita; Jarulis, Jarulis; Sipriyadi, Sipriyadi
MAXIMUS: Journal of Biological and Life Sciences Vol. 1 No. 2 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/maximus.v1i2.1150

Abstract

ABSTRAK Down Syndrome merupakan kelainan genetik autosomal karena kelebihan pada jumlah kromosom21 (Trisomi). Salah satu kekhasan pada penyandang down syndrome adalah mulut selalu terbuka, bibir memiliki celah dan relatif tebal. Tujuan dari penelitian ini adalah untuk menganalisis karakteristik pola sidik bibir pada penyandang down syndrome. Penelitian dilakukan pada bulan Desember 2021-Februari 2022 menggunakan metode wawancara dan pendataan langsung terhadap siswa penyandang down syndrome yang memenuhi syarat inklusi dari beberapa sekolah di Kota Bengkulu. Identifikasi pola sidik bibir mengacu pada klasifikasi menurut Suzuki dan Tsuchihashi. Data dianalisis menggunakan uji Chi-square pada program SPSS versi 22. Hasil penelitian menunjukkan bahwa pada siswa penyandang down syndrome ditemukan dua pola sidik bibir yaitu tipe I dan tipe II dengan pola dominan pada tipe I. Persentase pola sidik bibir tipe I pada siswa laki-laki sebesar 66,7% dan pada siswa perempuan 57,1%. Pola sidik bibir tipe II pada siswa laki-laki sebesar 33,3% dan pada siswa perempuan 42,9%. Berdasarkan hasil analisis menggunakan uji Chi-square, tidak terdapat hubungan antara pola sidik bibir dengan rasio tebal bibir dan panjang bibir, serta tidak terdapat hubungan antara pola sidik bibir dengan kemampuan berbicara, kemampuan berjalan, kemampuan menggenggam pena dan kemampuan menulis pada siswa penyandang down syndrome dari beberapa sekolah di kota Bengkulu. Pola sidik bibir dominan tipe I dan II yang ditemukan pada siswa penyandang down syndrome juga umum ditemukan pada non-down syndrome. Kata Kunci: Cheiloscopy, Down syndrome, Pola sidik bibir ABSTRACT An excess of chromosomal number 21 (Trisomy) causes the autosomal genetic disease known as down syndrome. One of the characteristics of people with down syndrome is that their lips have a gap and are rather thick, and their mouths are always open. This study's objective was to examine the characteristics of down syndrome students' lip print patterns. The study was carried out at multiple schools in Bengkulu City between December 2021 and February 2022 using interviews and direct data collecting on Down syndrome pupils with inclusion requirements. The classification of Suzuki and Tsuchihashi is used to identify lip print patterns. The Chi-square test was used to examine the data in SPSS version 22. Data were analyzed using the Chi-square test in the SPSS version 22. The findings indicated that students with Down syndrome had type I and type II lip print patterns, with type I predominating. Male students had a type I lip print pattern prevalence of 66.7%, compared to 57.1% for female students. 33.3% of male students have type II lip print patterns, compared to 42.9% of female students. According to the Chi-square analysis, there is no correlation between lip print patterns, the ratio of lip thickness to lip length, and speaking, walking, pen grasping, and writing abilities in down syndrome from various schools in Bengkulu city. Since the dominating lip print pattern of types I and II observed in down syndrome students is also frequently shown in non-down syndrome individuals, this pattern cannot be used to predict specific motor skills in down syndrome students. Keywords: Cheiloscopy, Down syndrome, Lip print patterns
Perbandingan Struktur Komunitas Cacing Tanah Megadrilli pada Ekosistem Alami dan Ekosistem Buatan Jody, Regina; Darmi, Darmi; Rizwar, Rizwar; Jarulis, Jarulis; Wibowo, Risky Hadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9262

Abstract

Earthworms are an important component of soil ecosystems. The presence of earthworms in a habitat can enhance the quality of the soil, both in its physical, chemical, and biological properties. This research has the purpose to analyze the comparison of earthworm density found in natural ecosystems and artificial ecosystems. The determination of location points is done using the Cluster Sampling method, which includes both natural and artificial ecosystems. Sampling is conducted using the line transect method along a 100-meter length in 20 plots. The earthworm collection method involves the use of square and hand sorting methods. Data analysis includes density values, frequency, diversity index, and the Morisita index, as well as statistical analysis using an independent sample T-Test to determine the significance of the difference in earthworm abundance between the two locations. Five species of megadrillic earthworms were identified, classified into two ecological categories: epigeic and endogeic. In the natural ecosystem, there were five species, namely Pheretima capensis (epigeic), P. corethururus (endogeic), Pheretima sp.1 (epigeic), Megascolex sp. (endogeic), and Pheretima sp.2 (epigeic). Meanwhile, in the artificial ecosystem, three species were found, which are P. capensis (epigeic), P. corethururus (endogeic), and Pheretima sp.2 (epigeic). The density of megadrillic earthworms in the natural ecosystem (567.8 individuals/m2) is significantly higher compared to the artificial ecosystem (242.5 individuals/m2). The relative frequency of earthworms in the natural ecosystem is divided into 3 categories: Rare (Assesory), Common (Constant), and Very Common (Absolute), whereas in the artificial ecosystem, there are 2 categories: Common (Constant) and Very Common (Absolute). The diversity index of earthworms in the natural ecosystem tends to be higher (H' = 1.236) than in the artificial ecosystem (H' = 0.885). The distribution pattern of megadrillic earthworms in the natural ecosystem is clustered, while in the artificial ecosystem, it is random.
Jenis-Jenis Tumbuhan Pakan Bajing Kelapa Callosciurus notatus di Desa Kepala Curup Bengkulu Kamilah, Santi Nurul; Jarulis, Jarulis; Eliza; Syarifuddin; Darmi
Konservasi Hayati Vol 19 No 1 (2023): APRIL
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v19i1.26598

Abstract

ABSTRAK Bajing kelapa Callociurus notatus merupakan hewan mamalia dari famili Sciuridae. Mereka bersifat arboreal, aktif mencari makan pada siang hari atau disebut juga sebagai hewan diurnal. Bajing kelapa dapat ditemukan pada berbagai tipe habitat. Mereka cukup toleran dengan kehadiran manusia sehingga sering dijumpai pada habitat dekat pemukiman penduduk. Penelitian terkait bajing kelapa ini dilakukan di Desa Kepala Curup Kecamatan Binduriang Kabupaten Rejang Lebong Bengkulu dengan tujuan untuk menginventarisasi tumbuhan apa saja yang dimanfaatkan sebagai makanan oleh bajing kelapa. Penelitian dilakukan pada bulan April hingga Juni 2020. Pengamatan dilakukan pada waktu aktivitas makan tinggi yaitu pada pukul 06.30-10.00 WIB dan dilanjutkan pada pukul 14.00-17.30 WIB menggunakan metode Ad libitum sampling. Berdasarkan hasil penelitian diketahui bahwa terdapat 9 jenis tumbuhan yang dimanfaatkan sebagai sumber pakan oleh bajing kelapa yaitu Singkong Manihot utilisima (bunga) 38,8%, pepaya Carica papaya (buah) 15,7%, kelapa Cocos nucifera (buah dan bunga) 13,3%, durian Durio zibethinus (buah) 10,9%, pisang Musa paradisiaca (buah) 9,4%, kemiri Aleurites moluccanus (buah) 5,3%, kapuk Ceiba petandra (daun muda) 3,3%, kopi Coffea Arabica (buah) 1,8%, dan Arenga pinnata (buah) 1,5%.. Dari keseluruhan jenis tumbuhan tersebut, bagian yang dikonsumsi berupa buah sebanyak 56%, bunga 41,6%, dan bagian daun muda 2,4%. Kata Kunci: Bajing kelapa, Callociurus notatus, Mamalia, Sciuridae ABSTRACT The plantain squirrel Callociurus notatus belongs to mammals, family of Sciuridae. They are arboreal animals, and then because they are actively during the day, they are classified as diurnal animals. Plantain squirrel can live in wide range of habitats. Since they can coexist with humans, they are frequently found near human residential areas. The purpose of this study was to identify kind of plant species that consumed by plantain squirrels in Kepala Curup village, Binduriang Subdistrict, Rejang Lebong District, Bengkulu. The study was conducted from April to June 2020, using the ad libitum sampling method. The observations began at 06.30-10.00 a.m. and ended at noon at 02.00-05.30 p.m. According to the study, plantain squirrels consume nine different types of plant, including Cassava / Manihot utilisima (flowers) 38.8%, papaya / Carica papaya (fruits) 15.7%, coconut / Cocos nucifera (fruit and flowers) 13.3%, durian / Durio zibethinus (fruits) 10.9%, banana / Musa paradisiaca (fruits) 9.4%, candlenut / Aleurites moluccanus (fruits) 5.3%, kapok / Ceiba petandra (young leaves) 3.3%, coffee / Coffea Arabica (fruits) 1.8%, and aren palm/ Arenga pinnata (fruits) 1.5%. Fruit was consumed 56%, flowers 41.6%, and young leaves by 2.4%. Keywords: Callociurus notatus, Mammals, Plantain squirrel, Sciuridae