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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Journal Pharmaceutical Science and Application (JPSA) Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Farmasi Udayana Jurnal Veteriner ANNALES BOGORIENSES JFIOnline

I Nengah Wirajana

Program Studi Kimia, Fakultas Sains Dan Matematika, Universitas Kristen Satya Wacana

Author-ID : 2921953

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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FRAKSINASI AMONIUM SULFAT PADA ENZIM ?-L-ARABINOFURANOSIDASE TERMOSTABIL I Nengah Wirajana; Ni Nyoman Tri Puspaningsih
Jurnal Kimia (Journal of Chemistry) Vol. 5, No. 2 Juli 2011
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.197 KB)

?-L-Arabinofuranosidases (EC 3.2.1.55) catalyze the hydrolysis ?-L-arabinofuranosidic bonds and act synergistically with other hemicellulases enzymes for the complete hydrolysis of hemicelluloses. Yeast Saccharomyces cerevisiae that considered as Generally Recognized As Safe (GRAS) was chosen for expression and secretion of thermostable ?-L-arabinofuranosidase (AbfA) from Geobacillus thermoleovorans IT-08 termofilik dengan teknologi DNA rekombinan. The extracellular enzyme from the secretion result in this recombinant yeast was precipitated with ammonium sulphate fractionation. Base on the result of measurement of the enzyme activity and the concentration of total protein showed that the specific activity of AbfA enzyme was the lowest at 40% saturation of ammonium sulfat, and the highest at 80% saturation of ammonium sulfat.

AMPLIFIKASI DAN IDENTIFIKASI MUTASI PADA FRAGMEN 0,5 KB GEN rpoB ISOLAT 134 Mycobacterium tuberculosis MULTIDRUG RESISTANT DENGAN METODE NESTED POLYMERASE CHAIN REACTION Made Dharmesti Wijaya; I Nengah Wirajana; Sagung Chandra Yowani
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 2 Juli 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Research has been conducted to amplify and identify mutations in the rpoB gene fragment, 0,5 kb, from the isolate 134 Mycobacterium tuberculosis multidrug resistance (MDR). Amplification was performed using the method nested polymerase chain reaction (nested PCR) while sequencing was conducted in one direction using forward inner primer. Nucleotide sequence obtained was translated into amino acids using MEGA4 program. Amplification of M.tuberculosis rpoB gene fragment, 0,5 kb, was successfully carried out and sequenced. Alignment result by using MEGA4 program showed that there had been missense mutations in the rpoB gene. It had two amino acids changes to rpoB protein, they were glutamic acid to aspartic acid at codon 418 and glutamine to arginine at codon 510.

SUBKLONING GEN -L-ARABINOFURANOSIDASE (abfA) DALAM VEKTOR EKSPRESI pYES2 I Nengah Wirajana; Eddy Bagus Wasito; H.M. Soekry Erfan Kusuma; Ni Nyoman Tri Puspaningsih
Jurnal Kimia (Journal of Chemistry) Vol. 4, No. 2 Juli 2010
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (512.632 KB)

A Gene encoding -L-arabinofuranosidase (abfa) that had been cloned into recombinant E. coliDH5a/pTP510 was subcloned into Saccharomyces cerevisiae yeast system. The aim of subcloning of abfA gene intoS. cerevisiae is to express the thermostable -L-arabinofuranosidase (AbfA) enzyme in the host that is often termedas Generally Recognized As Safe (GRAS), so that this enzyme earn the broader application like in food and beverageindustries. The gene of abfA was subcloned by the amplification of Polymerase Chain Reaction (PCR) from plasmidpTP510 templat. A pair of primers, pFSacI-Af (forward) and pXhoI-Af (reverse) from designed this research wasused for the amplifcation. The PCR condition was performed as follows : beginning denaturation at 94oC for 5 min;PCR cycle 30 times that consisted of denaturation (94oC for 1 min), annealing (55oC for 30 s), andpolymerization/extension at 72oC for 2 min; and than final extension at 72oC for 7 min. The abfA gene, resulted wasinserted between GAL1 promoter and CYT1 terminator in the pYES2 expression vector. This ligation product wastransformed into E. coli TOP10 host. Restriction analysis of recombinant plasmid from this construction, thedesignated as plasmid pY-Af, showed the expected size, about 7,4 kb, which was the summation of sizes of parentalplasmid ( 5,9 kb) and fragment of DNA insert (1,5 kb).

SKRINING SELULASE DARI TANAH HUTAN MANGROVE PANTAI SUWUNG BALI I Nengah Wirajana; Ketut Ratnayani; Darma Asih Yuliana
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 2 Juli 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Mangrove soil has high biodiversity and well known as potential location for enzymes exploration. The aim of direct screening for cellulase of mangrove soil is to find out the cellulase activity from mangrove soil. Mangrove soils were collected from three different locations labelled as A (8o43’38.20”SL), B (8o43’46,18”SL), and C (8o43’37,38”SL). The screening was conducted by Filter Paper Assay and Carboxymethyl Cellulose Assay methods. The results showed that cellulase activities can be measured directly from mangrove soil samples of Suwung Beach-Bali. The highest cellulase activities were 0,866 U/g soil by Filter Paper Assay and4,176 ± 0,630 U/g soil by Carboxymethyl Cellulose Assay, given by soil samples C.

PEMANFAATAN SILIKAT BENTONIT TERSUSPENSI NaCl, MgCl2, DAN CaCl2 UNTUK ISOLASI DNA METAGENOMIK DARI TANAH HUTAN MANGROVE Luh De Dwi Jayanthi; Ida Ayu Gede Widihati; I Nengah Wirajana
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The aim of this study was to compare the isolated metagenomic DNA of mangrove soil in Suwung Kauh Beach, Bali by utilizing suspended bentonite of NaCl, MgCl2 and CaCl2. The metagenomic DNA isolation was performed by directly lysis method from soil with lysis buffer and heat-shock, and then continued by DNA extraction with suspended bentonite of NaCl, MgCl2 and CaCl2. Preparation of suspended bentonite was done in 1 M NaCl, MgCl2 and CaCl2. The results of metagenomic DNA isolation were analysed by gel agarose electrophoresis and spectrophotometer Nano at wave length 230, 260, and 280 nm. The results of gel agarose electrophoresis showed that DNA was not detected in the first elution from all suspended bentonite, but DNA was only detected in the first supernatant before elution from Na-bentonite and Mg-bentonite suspended. The DNA bands were most thick in the supernatant before the elution of Na-bentonite. This result indicated that Na-bentonite adsorption to metagenomic DNA was lower than the other bentonite suspended. The DNA band was not detected in the first supernatant and the first elution from Ca-bentonite that indicated that DNA adsorption was stronger than the other and was not breakable from this suspended. The results of spectrophotometer nano showed that the metagenomic DNA isolated by suspended bentonite of NaCl, MgCl2 and CaCl2 were contaminated by humic acid and protein.

PENGARUH WAKTU INKUBASI TERHADAP AKTIVITAS LIPASE YANG DIINDUKSI DENGAN MINYAK JELANTAH PADA TANAH DARI HUTAN MANGROVE PANTAI SUWUNG KAUH BALI A A I A Mayun Laksmiwati; I Nengah Wirajana; Diah Suci
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 2 Juli 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Tanah hutan mangrove Pantai Suwung Kauh Bali dimanfaatkan sebagai sumber penghasil enzim, salah satunya adalah lipase. Penelitian ini bertujuan untuk mengetahui besarnya aktivitas lipase dengan dan tanpa penambahan minyak jelantah dan pengaruh waktu inkubasi terhadap aktivitas lipase tanah hutan mangrove. Metode titrasi asam-basa digunakan dalam pengukuran aktivitas lipase dengan waktu inkubasi selama 0,1,2,3,4,5,6 dan 7 hari. Hasil penelitian menunjukkan bahwa penambahan minyak jelantah dapat meningkatkan aktivitas lipase. Peningkatan aktivitas lipase diduga disebabkan oleh lipida yang terkandung dalam minyak jelantah dapat menginduksi lipase dari mikroorganisme lipolitik yang ada dalam tanah. Aktivitas lipase tertinggi diperoleh sebesar 0,0996 U/mL dengan penambahan minyak jelantah pada inkubasi hari ke-6. Aktivitas lipase dengan penambahan minyak jelantah dan dengan aerasi dihasilkan aktivitas lipase yang lebih tinggi sebesar 0,1250 U/mL dengan waktu inkubasi 5 hari. Waktu inkubasi berpengaruh nyata terhadap aktivitas lipase pada tanah hutan mangrove dengan dan tanpa penambahan minyak jelantah.

ANALISIS VARIASI NUKLEOTIDA DAERAH D-LOOP DNA MITOKONDRIA PADA SATU INDIVIDU SUKU BALI NORMAL Ketut Ratnayani; I Nengah Wirajana; A. A. I. A. M. Laksmiwati
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 1 Januari 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.578 KB)

Human mitochondrial DNA (mtDNA) has higher polimorfism level than nucleous genom, especially in theD-Loop region, which is a non coding region and the most polymorfic region in the mitochondrial genom. Theanalysis of variation of nucleotide sequence of D-Loop region can be used to determine the individual or ethnicidentity and also maternal familiar relationship. The research aims to determine nucleotide variant on Balineseindividue, which can be used as data base in determination of mtDNA genetical profile of Balinese ethnic in a biggerscale.To achieve the aims of the research, way the nucleotide sequence of one normal Balinese individue usingthe epithelia cells in the saliva. The methods were :1) the isolation of sample mtDNA, 2) the amplification of the DLoopregion of mtDNA by PCR, 3) sequencing and analysis of nucleotides sequence.The 0,4 kb fragment of the D-loop region mtDNA of the sample were successfully amplified, andsequenced of 402 pb. The research found 6 new variants or morfe different from Cambridge or Anderson sequence :variant 16223C®T, 16249T®C, 16259C®T, 16278C®T, 16316A®G, 16375C®A. The research also found thedeletion of T nucleotide on position 16362.

ALTERNATIF PENGGUNAAN SILIKA BENTONIT SEBAGAI PENGGANTI FENOL-KLOROFORM-ISOAMIL ALKOHOL DALAM EKSTRAKSI DNA SECARA LANGSUNG DARI TANAH HUTAN MANGROVE Khisan Qamariya; Wahyu Dwijani Sulihingtyas; I Nengah Wirajana
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Research on comparing the metagenomic DNA extraction from mangrove forest soil with phenol-chloroform-isoamyl alcohol and with silica bentonite was conducted. The purpose of this study was to find the differences in quality and quantity of metagenomic DNA extracted. The silica bentonite was compared to meet the Green Chemistry concept. The metagenomic DNA from these extractions were analyzed by agarose gel electrophoresis and UV-Vis spectrophotometry. The results of electrophoresis showed that metagenomic DNA extracted with silica bentonite were relatively higher in concentration than with phenol-chloroform-isoamyl alcohol; but integrity of their DNA were the same. The results of UV-Vis spectrophotometry analysis showed that A260/230 ratio of the phenol-chloroform-isoamyl alcohol extract had higher relative purity level to humic acid. The A260/280 ratio of the final elution of the silica bentonite extraction showed the highest relative purity level to protein.

KEMAMPUAN TANAH HUTAN MANGROVE SEBAGAI SUMBER ENZIM DALAM HIDROLISIS ENZIMATIK SUBSTRAT SEKAM PADI Ni Luh Md. Widayantini; I Nengah Wirajana; Putu Suarya
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 1 Januari 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Cellulase activity of mangrove forest soil from Suwung Kauh Beach in Bali has been reported in previous study. An enzymatic hydrolysis of rice husk with this mangrove forest soil as a source of enzymes was conducted. The aim of this study was to determine the ability of this mangrove forest soil as a source enzymes in hydrolyzing rice husk with and without delignification incubated in varied durations. The rice husk with and without delignification were mixed with mangrove forest soil and incubated at pH 7 and 29oC with a various incubation times of 0, 1, 2 , 3, and 4 weeks. Reducing sugar content of the results of incubation was measured by spectrophotometry using the Nelson-Somogyi method. The results of this study showed that the mangrove forest soil can hydrolyse the delignification rice husk, but could not degrade the rice husk without delignification. The highest reducing sugar content of 0,892 mg/100mL was resulted from hydrolysis of the delignification rice husk during one week. This result indicated that the mangrove forest soil as a source of cellulase had an ability in hydrolyzing the delignificated rice husk in pH 7 and 29oC incubated in one week.

ISOLASI DNA METAGENOMIK DARI MADU DENGAN DAN TANPA PENGAYAAN MEDIA LB (Luria-Bertani) I Gusti Ngurah Bagus Andre Hartawan; Made Arsa; Ni Komang Ariati; I Nengah Wirajana
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

DNA content in total honey microorganism can be used to study DNA fingerprint of honey. Metagenomic is a new approach to analyse complex genomes from unculturable total microorganism. The aim of this research was to get DNA metagenomic isolated directly from honey samples enriched with microorganisms growing media LB (Luria-Bertani) and without enrichment of microorganisms growing media LB in order to study DNA fingerprint of honey for the next research. The study was started with incubation of the LB enriched honey samples and honey without LB samples at variation time of 0, 24, 48, and 72 hours. Following the incubation process, the DNA metagenomic form both LB enriched and unenriched honey samples were isolated with direct cell lysis method. The DNA obtained was analyzed by spectrophotometer UV-Vis and agarose gel electrophoresis. The result showed that DNA metagenomic can be isolated from LB enriched honey and without LB enriched honey samples. Analysis using the agarose gel electrophoresis indicated that the metagenomic DNA obtained from both honey samples was still contaminated with RNA. The purity ratio of metagenomic DNA from both honey samples analysed using UV-Vis Spectrophotometer at ? 260/280 nm was between 1.2 to 1.6. This ratio suggested that the metagenomic DNA isolated from both samples were contaminated with protein and fenol.

Co-Authors A. A. B. Putra Anak Agung Istri Agung Mayun Laksmiwati D. Rizkiyanti Darma Asih Yuliana Dewi Andayani Farmawati Dewi, I Gusti Ayu Agung Gangga Samala Diah Suci Eddy Bagus Wasito Gusti A Malelak H.M. Soekry Erfan Kusuma I Gede Mahardika I Gusti Ngurah Bagus Andre Hartawan I Ketut Gede Dharma Dewantara I Made Agus Gelgel Wirasuta I P. J. D. A. Suartama I Putu Mahendra I W BUDIARSA SUYASA I Wayan Gede Gunawan I Wayan Suarsa I. A. Gede Widihati Ida Ayu Ratih Dwi Nugraha Putri Ida Bagus Amertha Putra Manuaba Ida Bagus Putra Manuaba Ida Bagus Putu Eristya Putra Ika Kurniawati Indra Juana Adikara Inten Hardianti Nizar Iryanti Eka Suprihatin James Sibarani Kazuo Sakka Ketut Ratnayani Khisan Qamariya Kimura, Tetsuya Kusuma, Soekry Erfan Luh De Dwi Jayanthi Luk Ketut Budi Maitriani M. Manurung Made Arsa Made Dharmesti Wijaya Made Rai Dwitya Wiradiputra Mirawati N.K.W. N. M. T. Juliasari N. W. Bogoriani Ni Komang Ariati Ni Komang Lia Wahyuni Ni Luh Md. Widayantini Ni Luh Putu Mustika Praptiwi Ni Made Suaniti Ni Made Yustikarini Ni Nengah Kartini Asih Ni Nyoman Tri Puspaningsih Ni Putu Ariantari Ni Putu Citra Anggryni Sugitha Ni Putu Frida Oktaningtias Widiarthi Ni Putu Rahayu Artini O. Ratnayani Oka Ratnayani P. Suarya Pradnyaniti, D.G Putra, Pramana Kumala Putri, Ni Wayan Prasanthi Swarna Putu Elvira Yulianthi Putu Suarya R. R. Sirait Ro’yal Aini Sagun Chandra Yowani Sakka, Kazuo Satriya Putra Prakoso Soekry Erfan Kusuma Tetsuya Kimura Wahyu Dwijani Sulihingtyas Y. H. Pratiwi

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