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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi ANNALES BOGORIENSES JFIOnline Jurnal Ilmiah Medicamento Annales Bogorienses

I Nengah Wirajana

Program Studi Kimia, Fakultas Sains Dan Matematika, Universitas Kristen Satya Wacana

Author-ID : 2921953

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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PENINGKATAN AKTIVITAS SELULASE PADA TANAH HUTAN MANGROVE PANTAI SUWUNG BALI DENGAN PENGAYAAN SUBTRAT SELULOSA JANUR KELAPA (Cocos nucifera) Ika Kurniawati; I Nengah Wirajana; I Gede Mahardika
Jurnal Kimia (Journal of Chemistry) Vol. 7, No. 1 Januari 2013
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The aim of this study is to determine the effect of coconut (Cocos nucifera) leaf enrichment to soil of mangrove forest of Bali Suwung coastal on the cellulase activity. The measurement of cellulase activity was conducted by CMC method (Carboxymethyl Cellulose Assay) on soil sample with and without enrichment of coconut leaf substrate in incubation time of 1, 2, 3, and 4 weeks. The results showed that the enrichment increased cellulase activity. In addition, cellulase activited the soil samples, increase with incubation times.

PERBANDINGAN KUALITAS DNA DENGAN MENGGUNAKAN METODE BOOM ORIGINAL DAN BOOM MODIFIKASI PADA ISOLAT Mycobacterium tuberculosis 151 Dewi Andayani Farmawati; I Nengah Wirajana; Sagung Chandra Yowani
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Tuberculosis (TB) is a disease caused by the Mycobacterium tuberculosis. Isolation DNA is a necessary step to obtain the bacterial chromosomal DNA used in the Polymerase Chain Reaction (PCR). Boom isolation method is an isolation method commonly used in isolation DNA of M. tuberculosis. In Bali, the isolation DNA of M. tuberculosis conducted at the Laboratory of Biomolecular FK UNUD uses boom modification method. This research aims to compare the quality of DNA produced by the Boom methods and Boom modification methods. This research was started with the isolation process using Boom method and Boom modification and subsequently amplified by PCR. Detection of PCR products was performed with electrophoresis method. DNA quality was determined by the thickness of bands DNA PCR product and purity analysis by spectrophotometry UV-Vis. The results obtained show that the quality of DNA Mycobacterium tuberculosis 151 isolate using Boom modification method (Laboratory of Biomolecular FK UNUD) is relatively better than Boom original methods (Boom et al, 1990).

PENGARUH PENAMBAHAN SUSU SKIM TERHADAP HASIL DNA METAGENOMIK DIISOLASI DARI TANAH HUTAN MANGROVE Ni Putu Frida Oktaningtias Widiarthi; Ketut Ratnayani; I Nengah Wirajana
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 1 Januari 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Cell lysis is the most important step for quality and quantity of metagenomic DNA isolated from an environmental samples. The aim of the research was to compare the quality (integrity and purity) of the metagenomic DNA isolated using the direct cell lysis method from mangrove forest soil with and without skim milk. The total of metagenomic DNA isolated from mangrove forest soil result was analyzed by the spectrophotometric UV-Vis method at ? 230, 260, and 280 nm; and also by using the agarose gel electrophoresis. The results showed that metagenomic DNA can be isolated from mangrove forest soil. The agarose gel electrophoresis results showed that the total DNA quality obtained by the direct cell lysis using buffer lysis with skim milk was relatively less fragmented and the band intensity of DNA was higher compared with direct cell lysis using buffer lysis without skim milk. The results of spectrophotometry indicated that the purity of DNA isolated with and without skim milk was not significantly different against the humic acid (ratio on A260/230). As shown by the A260/280 ratio, the total DNA isolated without skim milk had higher purity level than with skim milk.

AKTIVITAS EFFECTIVE MICROORGANISMS (EM) DALAM TANAH PERTANIAN ORGANIK YANG TERPAPAR KLORPIRIFOS D. Rizkiyanti; I N. Wirajana; I W. B. Suyasa
Jurnal Kimia (Journal of Chemistry) Vol. 14, No. 2 Juli 2020
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2020.v14.i02.p04

Salah satu cara untuk mengembalikan kesuburan tanah yang terpapar pestisida, seperti klorpirifos adalah dengan bioremediasi menggunakan Effective Microorganisms (EM). Tujuan penelitian ini adalah untuk mengetahui ketahanan mikroba EM dan aktivitas enzim pendegradasi klorpirifos dalam sampel tanah pertanian organik.Tanah pertanian organik yang digunakan sebelumnya telah terpaparEM dalam jangka waktu yang lama. Sampel tanah ini dibagi menjadi dua perlakuan, yaitu disterilkan dengan autoklaf dan tidak disterilkan (agar mikroba indigenous tetap hidup). Kedua sampel tanah ini selanjutnya ada yang tidak dan ditambahkan EM dengan waktu inkubasi 3, 5, dan 7 hari. Mikroba EM dapat bertahan terhadap paparan klorpirifos sampai konsentrasi 56 ppm. Fenomena aktivitas enzim pendegradasi klorpirifos sebesar 0,017 U/mL selama waktu inkubasi 7 hari dengan penurunan kadar klorpirifos sebesar 16,65% ditemukan pada sampel tanah steril dengan penambahan EM. Aktivitas enzim pendegradasi klorpirofos tertinggi, yaitu sebesar 0,039 U/mL selama waktu inkubasi 3 hari, dengan penurunan kadar klorpirifos sebesar 74,11% ditemukan pada sampel tanah tidak steril. Penurunan kadar klorpirifos tertinggi terdapat pada sampel tanah tidak steril sebesar 80,04% dengan waktu inkubasi 5 hari. Kata Kunci: klorpirifos, bioremediasi, mikroba EM (Effective Microorganisms)

PERBANDINGAN METODE UJI GULA PEREDUKSI DALAM PENENTUAN AKTIVITAS ?-L-ARABINOFURANOSIDASE DENGAN SUBSTRAT JANUR KELAPA (COCOS NUCIFERA) Y. H. Pratiwi; O. Ratnayani; I N. Wirajana
Jurnal Kimia (Journal of Chemistry) Vol.12 No.2 Juli 2018
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Pengujian gula pereduksi umumnya dilakukan dengan reagen asam 2,3-dinitrosalisilat (DNS) dan Nelson-Somogyi (NS) dalam penentuan aktivitas enzim pendegradasi polisakarida. Tujuan penelitian ini adalah untuk membandingkan metode uji gula pereduksi DNS dan NS dalam penentuan aktivitas ?-L-Arabinofuranosidase (AbfA) termostabil, dengan substrat janur kelapa (Cocos nucifera). Enzim AbfA diperoleh dari Saccharomyces cerevisiae rekombinan yang dikultivasi selama 3 hari pada suhu inkubasi 30 °C. Penentuan aktivitas enzim AbfA dilakukan pada kondisi pH 6, suhu 70 °C dan waktu inkubasi 15 menit. Hasil penelitian menunjukkan bahwa penentuan aktivitas enzim AbfA dengan metode NS lebih teliti atau memiliki standar deviasi yang jauh lebih kecil dibandingkan dengan metode DNS, namun kurva kalibrasi larutan standar metode DNS lebih linear dibandingkan metode NS.

PENGETAHUAN DAN PEMAHAMAN DINI NARKOBA DAN ZAT ADITIF PADA GENERASI MUDA SEBAGAI ASET BANGSA DI DESA MENGWI BADUNG N.M. Suaniti; I N. Wirajana; N.K. Ariati; M. Manurung
Buletin Udayana Mengabdi Vol 17 No 4 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Era globalisasi sebagai suatu peluang dan tantangan kaum muda Indonesia untuk berpartisipasi aktif memperoleh hal-hal yang tidak baik, salah satunya memahami pengetahuan awal tentang obat dan zat aditif yang memungkinkan dapat menyesatkan generasi muda. Drugs (narkotika dan obat / bahan berbahaya lainnya) dikenal sebagai obat-obatan yang disalahgunakan (narkotika, psikotropika dan zat adiktif lainnya) oleh Kementerian Kesehatan Republik Indonesia yang merujuk pada sekelompok senyawa yang umumnya memiliki risiko penambahan bagi pengguna. Selanjutnya, aditif makanan juga bisa menyebabkan keracunan. Badung adalah salah satu bagian dari dunia di mana tujuan wisata desa Mengwi menghubungkan daerah utara dan selatan menjadi rentan terhadap efek dari upaya pencegahan yang dilakukan sehingga salah satunya dengan memberikan pengetahuan obat-obatan dan aditif oleh otoritas yang relevan. Metode ini dilakukan dengan memberikan kuliah dan sesi tanya jawab dengan mengisi kuesioner pre dan posttest. Hasil kuesioner dianalisis dan menunjukkan peningkatan pemahaman obat dan aditif secara signifikan untuk menyebar secara merata pada generasi muda di desa Mengwi setelah kegiatan, tetapi kegiatan pendidikan ini perlu dilakukan secara berkala di setiap banjar dan diperluas di komunitas pedesaan lainnya di Bali.

PENGETAHUAN DAN PEMAHAMAN DINI NARKOBA DAN ZAT ADITIF PADA GENERASI MUDA SEBAGAI ASET BANGSA DI DESA MENGWI BADUNG N.M. Suaniti; I.N. Wirajana; N.K. Ariati; M. Manurung
Buletin Udayana Mengabdi Vol 16 No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.374 KB)

The era of globalization is an opportunity and challenges Indonesian youths to participate actively obtain in things that are not good, one of them understood early knowledge of drugs and additives that enable the younger generation can be misleading. Drugs (narcotics and drug/ hazardous materials) with another term drug (narcotics, psychotropic and other addictive substances) by the Ministry of Health of the Republic of Indonesia refers to a group of compounds generally have a risk of addition for users. Furthermore, food additives can also cause poisoning. Badung is one of part of the world where tourist destinations Mengwi village is connecting northern and southern regions become vulnerable to the effects of prevention efforts of prevention efforts undertaken so that one of them by giving lectures drugs and additives by the relevant authorities. The method is performed by giving a lecture and question and answer session with filling out the questionnaire pre and posttest. The results of the questionnaire were analyzed and showed an increase understanding of drugs and additives significantly to the spread evenly on the younger generation in Mengwi village after the activity, but this dedication activities need to be carried out periodically in each row and expanded in other rural communities in Bali

Construction of pY-Af Vector for Expression of Thermostable α-L-Arabinofuranosidase in Saccharomyces cerevisiae I Nengah Wirajana; Ni Nyoman Tri Puspaningsih; Eddy Bagus Wasito; Soekry Erfan Kusuma; Tetsuya Kimura; Kazuo Sakka
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

In this research, construction of expression vector for thermostable α -L-arabinofuranosidase in Saccharomyces cerevisiae was conducted. BJ1824 was conducted The Escherichia coli/S. cerevisiae shuttle vector, pYES2 was used as parental vector in construction. The abfA gene encoding α-L- arabinofuranosidase from Geobacillus thermoleovorans IT-08 was amplified by PCR, in which the plasmid pTP510 was used as a template. The amplification product was treated with SacI and XhoI and then subcloned to the pYES2 vector, which was previously digested with SacI and XhoI. The recombinant plasmid was designated as pY-Af and propagated first in E. coli Top 10, and then transformed into S. cerevisiae BJ1824. For α- Larabinofuranosidase (AbfA) production, the yeast transformants were grown in YNBG selective medium and YPG rich medium, using galactose as an inducer. The AbfA activity was assayed by measuring the amount of p-nitrophenol (pNP) released from p-nitrophenyl-α-L-arabinofuranoside (pNPA) substrate at pH 6.0 and 70 C for 30 min. The recombinant AbfA activity was detected in either of culture medium (0.98%), cellassociated (14.17%) and intracellular (84.85%) when recombinant yeast was grown in YPG rich medium.Key words: α-L-arabinofuranosidase; Saccharomyces cerevisiae; expression vector

Construction of pY-Af Vector for Expression of Thermostable α-L-Arabinofuranosidase in Saccharomyces cerevisiae Wirajana, I Nengah; Puspaningsih, Ni Nyoman Tri; Wasito, Eddy Bagus; Kusuma, Soekry Erfan; Kimura, Tetsuya; Sakka, Kazuo
Annales Bogorienses Vol. 14 No. 2 (2010): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

In this research, construction of expression vector for thermostable α-L-arabinofuranosidase in Saccharomyces cerevisiae BJ1824 was conducted. The Escherichia coli/S. cerevisiae shuttle vector, pYES2 was used as parental vector in construction. The abfA gene encoding α-L-arabinofuranosidase from Geobacillus thermoleovorans IT-08 was amplified by PCR, in which the plasmid pTP510 was used as a template. The amplification product was treated with SacI and XhoI and then subcloned to the pYES2 vector, which was previously digested with SacI and XhoI. The recombinant plasmid was designated as pY-Af and propagated first in E. coli Top10, and then transformed into S. cerevisiae BJ1824. For α-Larabinofuranosidase (AbfA) production, the yeast transformants were grown in YNBG selective medium and YPG rich medium, using galactose as an inducer. The AbfA activity was assayed by measuring the amount of p-nitrophenol (pNP) released from p-nitrophenyl-α-L-arabinofuranoside (pNPA) substrate at pH 6.0 and 70oC for 30 min. The recombinant AbfA activity was detected in either of culture medium (0.98%), cellassociated (14.17%) and intracellular (84.85%) when recombinant yeast was grown in YPG rich medium.

Aktivitas Antimikroba dan Antioksidan Jamur Laut yang Berasosiasi dengan Spons Pseudoceratina sp. dari Perairan Pantai Amed Adi, I Made Agus Kusuma; Witantri, Ni Komang Diah Eka; Wulandari, Ni Made Widya; Leliqia, Ni Putu Eka; Wirajana, I Nengah; Wibowo, Joko Tri; Ware, Ismail; Ariantari, Ni Putu
Jurnal Ilmiah Medicamento Vol 12 No 1 (2026): Jurnal Ilmiah Medicamento (In progress)
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v12i1.13356

Background: Marine fungi are organisms capable of growth and spore production in the marine environment and establishing symbiotic relationships with other marine organisms. Because of their high biochemical diversity, marine fungi are considered a potential source for the discovery of new natural compounds with various biological activities.Objective: This study aims to conduct molecular identification and assess the bioactivity potential of the methanol extract obtained from endophytic fungi associated with the marine sponge Pseudoceratina sp. from the Amed Coastal Waters, Bali.Methods: Eight marine fungi were isolated in this study and identified through molecular biology protocol as Aspergillus tamarii SP-3-1-4, Aspergillus nomiae SP-3-2 (A), Penicillium citrinum SP-3-2-1, Aspergillus protuberus SP-3-2-3, Aspergillus sydowii SP-3-2-4, Aspergillus sydowii RM NS SP-3B, Aspergillus clavatonanicus RM NS SP-3-2 A, and Aspergillus nomiae RM NS SP-3-2 B. The fungi were subsequently fermented on rice media containing salt and/or without salt. In the final stage of fermentation, secondary metabolites were extracted using ethyl acetate, followed by liquid-liquid extraction using methanol containing 10% water and n-hexane. The resulting methanolic extracts were subjected to phytochemical analysis and bioassays.Results: The phytochemical screening results of the methanol extracts showed that all extracts contained alkaloids. Among the tested extracts, the extract obtained from the fermentation of the fungus A. protuberus SP-3-2-3 on rice medium with the addition of salt showed the highest activity against Methicillin-resistant Staphylococcus aureus ATCC 3351 with an inhibition zone diameter of 7.99±0.20 mm. The fungus A. sydowii SP-3-2-4 cultured with and without salt in rice media exhibited the most potent antioxidant capacity, with IC50 values of 37.02±1.12 and 32.48±0.81 µg/mL. Conversely, A. nomiae SP-3-2 (A), fermented without salt, displayed the highest toxicity with an LC50 value of 0.88±0.69 µg/mL.Conclusion: Based on the pharmacological potential of extracts produced by the marine fungi associated with the sponge Pseudoceratina sp. found in the present study, identification of bioactive secondary metabolites and their mode of action is propitious for further investigation.

Co-Authors A. A. B. Putra Adi, I Made Agus Kusuma Anak Agung Istri Agung Mayun Laksmiwati D. Rizkiyanti Darma Asih Yuliana Dewi Andayani Farmawati Dewi, I Gusti Ayu Agung Gangga Samala Diah Suci Eddy Bagus Wasito Gusti A Malelak H.M. Soekry Erfan Kusuma I Gede Mahardika I Gusti Ngurah Bagus Andre Hartawan I Ketut Gede Dharma Dewantara I Made Agus Gelgel Wirasuta I P. J. D. A. Suartama I Putu Mahendra I W BUDIARSA SUYASA I Wayan Gede Gunawan I Wayan Suarsa I. A. Gede Widihati Ida Ayu Ratih Dwi Nugraha Putri Ida Bagus Amertha Putra Manuaba Ida Bagus Putra Manuaba Ida Bagus Putu Eristya Putra Ika Kurniawati Indra Juana Adikara Inten Hardianti Nizar Iryanti Eka Suprihatin James Sibarani Joko Tri Wibowo, Joko Tri Kazuo Sakka Ketut Ratnayani Khisan Qamariya Kimura, Tetsuya Kimura, Tetsuya Kusuma, Soekry Erfan Kusuma, Soekry Erfan Luh De Dwi Jayanthi Luk Ketut Budi Maitriani M. Manurung Made Arsa Made Dharmesti Wijaya Made Rai Dwitya Wiradiputra Mirawati N.K.W. N. M. T. Juliasari N. W. Bogoriani Ni Komang Ariati Ni Komang Lia Wahyuni Ni Luh Md. Widayantini Ni Luh Putu Mustika Praptiwi Ni Made Suaniti Ni Made Yustikarini Ni Nengah Kartini Asih Ni Nyoman Tri Puspaningsih Ni Putu Ariantari Ni Putu Citra Anggryni Sugitha Ni Putu Eka Leliqia Ni Putu Frida Oktaningtias Widiarthi Ni Putu Rahayu Artini O. Ratnayani Oka Ratnayani P. Suarya Pradnyaniti, D.G Putra, Pramana Kumala Putri, Ni Wayan Prasanthi Swarna Putu Elvira Yulianthi Putu Suarya R. R. Sirait Ro’yal Aini Sagun Chandra Yowani Sakka, Kazuo Sakka, Kazuo Satriya Putra Prakoso Soekry Erfan Kusuma Tetsuya Kimura Wahyu Dwijani Sulihingtyas Ware, Ismail Witantri, Ni Komang Diah Eka Wulandari, Ni Made Widya Y. H. Pratiwi

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