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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Veteriner Padjadjaran Journal of Dentistry Communications in Science and Technology ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi Jurnal Ilmu Kefarmasian Indonesia Indonesian Journal of Applied Research (IJAR) Jurnal Sylva Lestari Jurnal Kedokteran Hewan
Eva Harlina
Division Of Pathology, School Of Veterinary Medicine And Biomedical Sciences, IPB University, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Molecular Docking of the Bioactive Compound Physalis angulata as an Activator of Superoxide Dismutase 1 Wilujeng, Retno; Harlina, Eva; Purwono, Rini Madyastuti; Andrianto, Dimas
Indonesian Journal of Applied Research (IJAR) Vol. 6 No. 2 (2025): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v6i2.737

Abstract

Physalis angulata was known for its pharmacological potential, including antioxidant activity, yet the specific bioactive compounds responsible for enhancing endogenous antioxidant enzymes, such as superoxide dismutase 1 (SOD 1), remained unclear. This study aimed to identify and evaluate the active compounds of P. angulata as natural SOD 1 activators using an In silico molecular docking approach. Forty-nine ligands derived from the active constituents of P. angulata were screened based on Lipinski’s Rule of Five and ADMET properties. Molecular docking was performed using the SOD 1 protein (PDB ID: 5YTO), and docking validation yielded an RMSD value of 0.005 Å. Among the test ligands, withanolide exhibited the most favorable binding energy (−7.011 kcal/mol) and the lowest inhibition constant (7.0 pM), forming strong interactions with key catalytic residues of the enzyme. These findings indicated that withanolide had promising potential as a natural SOD 1 activator, providing a basis for future antioxidant drug discovery.
Histopatologi hati ayam broiler (Gallus domesticus): sebuah model forensik veteriner pada 48 jam pasca kematian Az Zahra, Shafiyyah; Harlina, Eva; Subangkit, Mawar; Wiranti, Rahayu Woro; Priosoeryanto, Bambang Pontjo
ARSHI Veterinary Letters Vol. 7 No. 4 (2023): ARSHI Veterinary Letters - November 2023
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.7.4.79-80

Abstract

Ilmu forensik veteriner belum mendapat perhatian yang memadai di Indonesia, sehingga perlu ditingkatkan pengembangannya. Penelitian ini bertujuan mempelajari ilmu forensik veteriner melalui histopatologi hati ayam broiler 48 jam pasca kematian. Ayam broiler sebanyak 75 ekor berumur 7 hari dieutanasia dan dibagi menjadi 25 kelompok berdasarkan jam nekropsi pasca-eutanasia (n=3). Kadaver ayam dinekropsi setiap 2 jam postmortem, organ hati dikoleksi untuk dibuat potongan histopatologi dan diwarnai dengan Hematoksilin-Eosin (HE). Histopatologi hati evaluasi dan hasil yang didapat dianalisis menggunakan software ImageJ versi 1.53a. Hubungan peubah histopatologi dengan waktu postmortem dianalisis dengan metode Pearsons. Hasil penelitian menunjukkan bahwa pembusukan dimulai pada jam ke-18 post mortem yang ditandai dengan jumlah inti sel hepatosit, jam ke-20 ditemukan bakteri pembusuk, dan jam ke-22 terjadi peningkatan jarak antar hepatosit. Berdasarkan nilai korelasi Pearson, jumlah inti sel hepatosit, jarak antar hepatosit dan keberadaan bakteri pembusuk mempunyai hubungan kuat hingga sangat kuat dengan waktu postmortem sehingga hasil ini dapat menjadi parameter penentu waktu kematian (Post Mortem Interval/PMI) pada hewan.
THE EFFECTIVENESS OF STEM CELL THERAPY IN MYOCARDIAL INFARCTION REVIEWED FROM UREUM, CREATININE AND KIDNEY HISTOPATHOLOGY LEVELS Rahmi, Nur; Gunanti, Gunanti; Noviana, Deni; Harlina, Eva
Jurnal Kedokteran Hewan Vol 18, No 3 (2024): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i3.30831

Abstract

This study aims to study the effectiveness of single placental stem cell therapy or cardiomyocyte combination in pigs experiencing myocardial infarction in terms of urea, creatinine and kidney organ histopathology values. A total of 9 pigs were divided int o three groups: without therapy (K1), single placental stem cell therapy (K2), and cardiomyocyte coculture combination therapy (K3). All pigs underwent circumflex artery ligation of the proximal coronary artery branch to stimulate cardiac ischemia and therapy was given after myocardial infarction and ST-elevation were formed. The pigs were then treated for eight weeks, euthanized and necropsied for kidney organ removal. The re sults showed a significant decrease in urea and creatinine values (P0.05) in K2 and K3 as compared to K1. Histopathology results showed a decrease in glomerular atrophy values (K2: 10.260.93 and K3: 12.441.21), tubular necrosis (K2: 13.673.76 and K3: 17.230.55), and tubular dilation (K2: 16.361.26 and K3: 18.031.76) and these values were significantly different than those observed in K1. It was concluded that the administration of placental stem cell therapy or combined with cardiomyocyte coculture could reduce urea, creatinine values, and improve kidney tissue histopathologically.
Antidiabetic Activities of Agarwood (Aquilaria malaccensis) Leaf Extracts via Enhanced Insulin Secretion in BRIN-BD11 Pancreatic Beta-Cells Prayogo, Yanico Hadi; Sari, Rita Kartika; Hamidi, Zikri; Syafii, Wasrin; Harlina, Eva; Prasetyo, Bayu Febram
Jurnal Sylva Lestari Vol. 13 No. 3 (2025): September
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v13i3.1197

Abstract

Agarwood leaves (Aquilaria malaccensis), a non-timber forest products, shows significant potential as a source of antidiabetic compounds. This study aims to evaluate the antidiabetic activity of agarwood leaves and their cytotoxicity on pancreatic beta-cells, as well as predict their compound bioactivity through an in silico approach. The leaves were extracted using ethanol, water, and a mixture of ethanol–water with the assistance of ultrasound irradiation. The extracts were then tested in vitro for their antidiabetic potential by assessing their ability to inhibit the alpha-glucosidase enzyme and their effect on insulin secretion, as well as their cytotoxicity on BRIN-BD11 pancreatic beta-cells. The phytocompounds in the extract were identified using liquid chromatography-mass spectrometry, and their binding behavior was studied by in silico molecular docking. Among the three, the ethanol–water extract showed the highest extraction yield. Cytotoxicity assays revealed that the ethanol–water extract was cytotoxic at high concentrations (1000 µg/mL), but safe at lower concentrations. The alpha-glucosidase inhibition was relatively weak. Nevertheless, the extracts significantly stimulated insulin secretion in BRIN-BD11 cells up to fivefold compared to untreated cells. In silico studies indicated that xanthone glycoside, flavonoid glycoside, and coumarin compounds exhibit strong binding affinities to multiple insulin-secretion-related proteins. These findings suggest that agarwood leaf extract, particularly ethanol–water extract, possesses promising antidiabetic activity through an insulinotropic mechanism. Keywords: agarwood, alpha-glucosidase, insulinotropic, molecular docking
In-vitro, In-vivo, and In-silico Studies of Insulinotropic Activity and Cytotoxicity of Mahagony Seed Extracts and Its Nanophytosome Hamidi, Zikri; Sari, Rita Kartika; Prayogo, Yanico Hadi; Syafii, Wasrin; Kasmi, Adinda Zahra Marissa; Prasetyo, Bayu Febram; Harlina, Eva; Wahyudi, Setyanto Tri
Communications in Science and Technology Vol 10 No 2 (2025)
Publisher : Komunitas Ilmuwan dan Profesional Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21924/cst.10.2.2025.1676

Abstract

This present study set out with the objective of evaluating the effect of ethanol–water solvent composition on extraction yield, insulinotropic activity (IA), cytotoxicity, and phytochemical profile (PP) of mahogany (Swietenia mahagoni) seed (MS) extracts. It also sought to study in silico and encapsulate the best extract, and to assay the antidiabetic of the extract and its nanophytosome. The extraction of MSs was achieved through the utilization of ethanol, ethanol–water (1:1), and water through ultrasound–assisted extraction. The extracts of IA and cytotoxicity were assayed in vitro using BRIN–BD11 cell lines and analyzed using LC-MS. The most effective extract was identified for its active compounds using molecular docking, encapsulation, characterization, and testing for its antidiabetic properties in vivo. The highest yield was observed in the water extract (MW), followed by the ethanol–water extract (MEW) and the ethanol extract (ME). MEW and MW demonstrated low level of cytotoxicity, with MEW exhibiting the highest level of IA. An in silico study identified four key antidiabetic compounds through the IA mechanism. The study demonstrated that MEW and its nanophytosome (250 mg/kg mouse weight) effectively reduced blood glucose level, and enhanced both erythrocyte function, and insulin secretion. This finding provides a new perspective in the development of natural-based antidiabetic agents through the nanophytosome formulation of mahogany seed extract.
Co-Authors . Jumari A.S. Satyaningtijas Abel Tasman Yuza, Abel Tasman Alauddin, Daffa Rizal Dzulfaqaar Alvernita, Gita Andri Hardianto Ardi Sandriya Az Zahra, Shafiyyah Bambang Pontjo Priosoeryanto Bayu Febram Prasetyo Bernadi, Willy Deni Noviana DIMAS ANDRIANTO Dwi Utari Rahmiati Erni H Purwaningsih Erni H Purwaningsih Gita Alvernita Gunanti . Gunanti Gunanti Hamidi, Zikri HERA MAHESHWARI Heryudianto Vibowo Huda S Darusman IETJE WIENTARSIH Iis Ismawati Ikrar Trisnaning Hardi Utami Kasmi, Adinda Zahra Marissa Kiagus Dahlan KUSMARDI KUSMARDI Lanniari, Nafrina Lina Noviyanti Sutardi Madyastuti, Rini Mega Safithri MUHAMMAD ADIB MUSTOFA MULYATI EFFENDI Okabayashi, Tamaki Prayogo, Yanico Hadi Putu Angga Wiradana Putu Eka Sudaryatma Rachmi Ridho Raden Mohamad Herdian Bhakti Rahmi, Riska Asria Sa'adatur Retno Wulansari Riski Rostantinata Riski Rostantinata Rita Kartika Sari Rizki Rostantinata Rostantinata, Riski S Darusman, Huda SANTOSO CORNAIN Sa’diah, Siti Setyanto Tri Wahyudi Setyo Widodo Setyo Widodo Siti Subangkit, Mawar Surachmi Setiyaningsih TATI NURHAYATI Umi Cahyaningsih Vetnizah Juniantito Vici Eko Handayani Waras Nurcholis Wasrin Syafii Wilujeng, Retno Wiranti, Rahayu Woro YENI KEZIA BEKALANI