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All Journal Jurnal Kesehatan Lingkungan indonesia Poltekita : Jurnal Ilmu Kesehatan Medical Laboratory Analysis and Sciences Journal Jurnal teknologi Kesehatan Borneo MAHESA : Malahayati Health Student Journal Innovative: Journal Of Social Science Research Jurnal Laboratorium Khatulistiwa MEDICA (International Medical Scientific Journal) ELECTRON (Journal of Science and Technology)
Laila Kamilla
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The Effect of Different Soaking Times of Gambir (Uncaria gambir (Hunter) Roxb.) on the Examination of Ascaris lumbricoides Eggs as an Alternative Stain to Eosin Rahmatika, Tantri; Nuswantoro, Ari; Kamilla, Laila
MEDICA (International Medical Scientific Journal) Vol. 7 No. 2 (2025): MEDICA (International Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i2.512

Abstract

Ascaris lumbricoides is a type of helminth that can infect humans. One laboratory method for its examination is the use of 2% eosin staining with the direct slide method. However, eosin poses potential hazards to both the environment and human health, thus prompting the need for an alternative, eco-friendly staining agent. Gambir (Uncaria gambir (Hunter) Roxb.) contains red catechu, which imparts a red coloration to solutions. This study aims to evaluate the staining differences based on varying soaking times of Gambir extract for staining Ascaris lumbricoides eggs as a natural alternative to eosin. This research is a quasi-experimental study using purposive sampling. The Gambir samples were soaked in 96% ethanol for 1 hour, 1 hour 30 minutes, and 2 hours. Each treatment was replicated 9 times, resulting in a total of 27 samples. The staining was conducted using the direct slide method to examine Ascaris lumbricoides eggs. The results showed that Gambir soaked for 1 hour yielded a staining quality of 41.67%, 1 hour 30 minutes resulted in 68.51%, and 2 hours resulted in 87.96%, while the 2% eosin control achieved a staining quality of 100%. Based on the results of this study on the variation in soaking time of gambier (Uncaria gambir (Hunter) Roxb.) as an alternative dye to eosin in the examination of Ascaris lumbricoides eggs, it can be concluded that the staining success rate increases with longer soaking durations.
Identification of Pathogenic Bacteria in Tofu Water and Sugarcane Water in Pontianak City Hermantika, David Deovia Triputra; Ihsan, Bagus Muhammad; Kamilla, Laila; Khayan, Khayan; Hanif, Muhammad Ifham
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 4 (2024): February
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i4.3336

Abstract

Tofu water and sugar cane juice are trendy drinks, especially in Pontianak City. Pathogenic bacterial contamination in tofu water and sugar cane juice can be a severe threat if the immune system is weak. This research aims to identify pathogenic bacteria in Pontianak City tofu water and sugar cane water. The methods used are culture methods and biochemical tests. The research samples were tofu water and sugar cane juice sold in the community. The sample was selected using a purposive sampling technique. In this study, the bacteria found in the identification of bacteria in tofu water and sugar cane water were Klebsiella pneumoniae, Shigella dysenteriae, Staphylococcus aureus, Enterobacter aerogenes, Salmonella typhi, and Escherichia coli. Bacteria dominate, while in sugarcane juice, it is dominated by Escherichia coli, followed by Klebsiella pneumoniae. The research results can also be the basis for better policies and preventive measures related to the safety of the quality of tofu water and sugar cane juice in Pontianak City. Therefore, this research can be relevant to environmental protection and public health in Pontianak.
Isolasi, Identifikasi Dan Profil Resistensi Antibiotik Bakteri Staphylococcus Aureus Dari Air Tahu Yang Dijual Di Berbagai Kecamatan Di Kota Pontianak Fidelia Putri, Angelyna; Kamilla, Laila; Tumpuk, Sri
Innovative: Journal Of Social Science Research Vol. 4 No. 4 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i4.11995

Abstract

Air tahu merupakan minuman hasil olahan dari bahan baku kacang kedelai. Air tahu banyak diminati oleh masyarakat Kota Pontianak karena harganya terjangkau. Lokasi penjualan, proses pembuatan, alat yang digunakan saat menyajikan, hingga higien penjual, jika tidak diperhatikan dan dijaga dapat menjadi sumber kontaminasi mikroorganisme terhadap air tahu tersebut, misalnya oleh Staphylococcus aureus. Penelitian ini bertujuan untuk mengidentifikasi dan memberikan gambaran profil resistensi antibiotik bakteri Staphylococcus aureus dari air tahu yang dijual di wilayah Kecamatan Pontianak Tenggara, Pontianak Selatan, dan Pontianak Kota. Penelitian yang dilakukan berbentuk deskripsi observasi dan berdesain cross sectional dengan teknik pengambilan sampel purposive sampling didapatkan sebanyak 43 sampel air tahu. Identifikasi dilakukan dengan metode kultur dan uji biokimia, sedangkan uji resistensi dilakukan dengan metode Kirby-Bauer terhadap empat cakram antibiotik, yaitu penisilin G (10 unit), kloramfenikol (30 µg), amoksisilin (30 µg), dan siprofloksasin (5 µg). Hasil identifikasi didapatkan 9 dari 43 (21%) sampel terkontaminasi bakteri Staphylococcus aureus dan hasil uji resistensi didapatkan 9 dari 9 sampel (100%) resisten terhadap penisilin, 1 dari 9 sampel (11,1%) resisten terhadap siprofloksasin, 2 dari 9 sampel (22,2%) resisten terhadap kloramfenikol, dan 4 dari 9 sampel (44,4%) resisten terhadap amoksisilin.
Normal dan Dislipidemia: Profil Lipid Pasien Hipertensi- Prolanis di Kota Pontianak Sen, Sharen; Nuswantoro, Ari; Kamilla, Laila; Aprilia, Dinasti
MAHESA : Malahayati Health Student Journal Vol 4, No 1 (2024): Volume 4 Nomor 1 (2024)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v4i1.13266

Abstract

ABSTRACT The primary cause of cardiovascular disease (CVD) is hypertension (HT). Through the Prolanis program, BPJS treats HT patients in Indonesia through various activities, including physical activity and monitoring lipid profiles, which until now have not been widely reported. The aim of this study was to describe the lipid profile of HT patients participating in Prolanis in Pontianak City. The samples are the medical records from 110 patients chosen using the total sampling method in 2022 at the Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak. Information gathered from 99 women and 11 men revealed that 80.8% of the women had hypercholesterolemia; triglycerides and HDL were mainly within normal ranges; LDL ranged from normal (13.6%) to extremely high (8.2%); and the ratios of cholesterol to HDL and triglycerides to HDL were mainly normal. One of the Prolanis program's goals, physical exercise, appears to have an impact on the patient's typical lipid profile. Nonetheless, the fact that some people have dyslipidemia raises awareness of the risk of CVD and supports the necessity of keeping this program in place. Keywords: Cardiovascular Disease, Lipid Profile, Hypertensive Patients, Prolanis  ABSTRAK Penyebab utama penyakit kardiovaskular (CVD) adalah hipertensi (HT). Melalui program Prolanis, BPJS menangani pasien HT di Indonesia melalui berbagai kegiatan, termasuk aktivitas fisik dan pemantauan profil lipid, yang hingga saat ini belum banyak dilaporkan. Tujuan dari penelitian ini adalah untuk mendeskripsikan profil lipid pasien HT yang mengikuti Prolanis di Kota Pontianak. Sampel penelitian ini adalah rekam medis dari 110 pasien yang dipilih dengan menggunakan metode total sampling pada tahun 2022 di Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak. Informasi yang dikumpulkan dari 99 wanita dan 11 pria menunjukkan bahwa 80,8% wanita mengalami hiperkolesterolemia; trigliserida dan HDL sebagian besar dalam kisaran normal; LDL berkisar dari normal (13,6%) hingga sangat tinggi (8,2%); dan rasio kolesterol terhadap HDL serta trigliserida terhadap HDL sebagian besar dalam kisaran normal. Salah satu tujuan program Prolanis, yaitu latihan fisik, tampaknya berdampak pada profil lipid pasien. Meskipun demikian, fakta bahwa beberapa orang memiliki dislipidemia meningkatkan kesadaran akan risiko CVD dan mendukung perlunya mempertahankan program ini. Kata Kunci: Penyakit Kardiovaskular, Profil Lipid, Pasien Hipertensi, Prolanis
Anti-Inflammatory Activity Test of Tapak Liman (Elephantopus scaber L.) Leaf Extract Using Red Blood Cell Membrane Stabilization Method Wahdaniah, Wahdaniah; Yanti, Rahmi; Kamilla, Laila; Salim, Maulidiyah
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2135

Abstract

Inflammation is the reaction of living tissue to trauma or infection, both in acute and chronic conditions. One plant with anti-inflammatory potential is the tapak liman leaf (Elephantopus scamber L.), which contains secondary metabolites such as phenolics, flavonoids, tannins, alkaloids, steroids/treponoids, and saponins. The purpose of this study was to determine the anti-inflammatory activity of Elephantopus scamber L. leaf extract at concentrations of 0.005%, 0.01%, 0.02%, 0.04%, and 0.08% using the red blood cell membrane stability method. Anti-inflammatory activity showed that 70% ethanol extract of tapak liman leaves had low anti-inflammatory activity at a concentration of 0.005% with a stability of 98.00%, while the highest activity was found at a concentration of 0.08% with a stability of 99.65%. Microscopic examination showed that at concentrations of 0.005%, 0.01%, 0.02%, 0.04%, and 0.08%, the administration of tapak liman extract could restore the normal shape of erythrocytes. The Kruskal-Wallis test yielded a significance value of 0.000, which is less than 0.05, meaning that Ha is accepted. It can be concluded that there are differences in the anti-inflammatory activity of tapak liman leaf extract (Elephantopus scamber L.) at concentrations of 0.005%, 0.01%, 0.02%, 0.04%, and 0.08%.
Co-Authors Ananda, Fanny Rizki Anisa Fitri Midani Aprilia, Dinasti Ari Nuswantoro Dewi, Bastiana Djohan, Herlinda Eka Susanti Fadilla, Alda Nur Fidelia Putri, Angelyna Franciska, Maria Gestiwana, Oksa Hanif, Muhammad Ifham Hendra Budi Sungkawa, Hendra Budi Hermantika, David Deovia Triputra Ihsan, Bagus Muhammad Imma Fatayati Indah Purwaningsih Jajar Pramata Syari Khayan Khayan Lestari, cik Verra Budi Linda Triana Maretalinia, Maretalinia Maulidiyah Salim mawaddah, mutia putri nirnadia, nirnadia Novi Wahyuni Nur Endah Wahyuningsih Nurhadiah, Nurhadiah Nurhayati, Etiek Pusaka, Semerdanta Qorina Miranti Rahmatika, Tantri Ramadhanty, Khairida Ratulangi, Agus Dimas Sabrina Azani, Aulia Saputra, Daniel Angkasa Sari, Emilda Sen, Sharen Sri Tumpuk Sugiono Sugiono Sutriswanto, Sutriswanto Suwandi, Edy Syari, Jajar Trifani, Paula Jaenne Tumpuk, Sri Wahdaniah Wahyudi YANTI, RAHMI