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All Journal HAYATI Journal of Biosciences Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Jurnal Ilmu dan Kesehatan Hewan Pastura; Journal Of Tropical Forage Science Jurnal Veteriner E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) agriTECH Cakrawala Repositori Imwi Nandur
I Nyoman Dibia
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Veterinary Other

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Diagnosis and Molecular Marker Analysis of Bali’s Rabies Virus Isolates (DIAGNOSIS DAN ANALISIS PENANDA MOLEKULER VIRUS RABIES ISOLAT BALI) I Nyoman Dibia; Bambang Sumiarto; Heru Susetya; Anak Agung Gde Putra; I Gusti Ngurah Kade Mahardika; Helen Scott-Orr
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The direct fluorescent antibody test (dFAT) was recommended by both World Health Organization(WHO) and Office International des Epizooties (OIE) as a standard diagnostic technique for rabies. Sincethe outbreak of rabies in Bali, it was ascertain the importance to develop a reverse transcriptase-polymerasechain reaction (RT-PCR) technique with specific primers as an alternative diagnostic method. The aim ofthis study was to develop a RT-PCR technique for rabies diagnosis in animals and find out the molecularmarker of Bali’s rabies virus (BRV) isolates based on the sequence of nucleoprotein (N) gene. Brainsamples were obtained during 2009 from 14 suspected rabid dogs and one cattle, where rabies viruseswere isolated. The dFAT was used to detect the presence of rabies viral antigen. Ribonucleic acid (RNA) ofrabies viruses was extracted with TRIzol reagent. Fragment of N gene was amplified using one-step RTPCRmethod with specifically-designed primer pairs and sequenced using ABI automatic sequencer. Multiplealignment of nucleotide and deduced amino acid sequences were analyzed using ClustalW of MEGA 4.0program. This study found that twelve out of fifteen animal brain samples confirmed as rabies by dFAT.Similarly, a single band of 1215 bp PCR product for rabies virus was also detected in twelve out of twelve(100%) dFAT rabies positive samples. It is therefore evident that alternative diagnostic of rabies inanimals can be established using RT-PCR technique. The results showed that the RT-PCR has a very highagreement with dFAT. Polymorphic sites of N gene of twelve BRV isolates were identified at the position186, 501, 801, 840, 1068 and 1153. Bali’s rabies virus isolates have conserved amino acid (isoleucine)alterations at position 308 (open reading frame). Isoleucine distinguished between all Bali’s isolates andthe all of isolates from other area of Indonesia and other part of the world. This finding significantlydifferent as compared to other rabies virus isolates from other part of Indonesia or the world documentedon the GenBank. Accordingly it is proposed that it can be used as molecular marker and believed to be thefirst study of molecular marker of rabies virus in Indonesia.
Faktor-Faktor Risiko Rabies pada Anjing di Bali (RISK FACTORS ANALYSIS FOR RABIES INDOGS IN BALI) I Nyoman Dibia; Bambang Sumiarto; Heru Susetya; Anak Agung Gde Putra; Helen Scott-Orr
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The efforts to eradicate rabies in Bali have been done for more than three years. However, therabiescases is still spreading. Thus, rabies virus continues to infect humans. A case-control study wasconducted to identify the risk factors associated with rabid dog in Bali. Cases were defined as dogsconfirmed having rabies by direct fluorescent antibody test (dFAT). Determination of sample amount ineach district was taken proportionally and samples were taken by using simple random sampling. A totalof 51 rabid dog cases between 2010 and 2011 and 102 uninfected rabies dogs as control were used in thisstudy. Possible associated factors were obtained by doing questionnaire. The data were subsequentlyanalyzed using chi-square (X2) and odds-ratio (OR) for possible association, which were ultimately analyzedby means of logistic regression to build up of model. This study revealed that factors associated with rabiddog were the status of rabies vaccination (X2= 55.538; P= 0.000; OR= 19.133; 95% CI= 8.015<OR<45.678),contact with other dog (X2= 43.659; P= 0.000; OR= 12.551; 95% CI= 5.541<OR<28.430),condition of dog(X2= 9.994; P= 0.002; OR= 3.019; 95% CI= 1.504<OR<6.058),number of raised dog (X2= 9.284; P= 0.002;OR= 2.962; 95% CI= 1.455<OR<6.027), and veterinary care (X2= 5.258; P= 0.022; OR= 2.444; 95% CI=1.125<OR<5.310). It was found an appropriate logit model to estimate probability of rabid dog events inBali province as follows : Logit Pr (rabies=1| x) = - 4.413 + 3.919 (status of rabies vaccination) + 3.457(contact with other dog). This study is expected to be used as a reference in order to improve rabies controleffectiveness in Bali.
Evaluasi Kesesuaian Lahan Beberapa Komoditas Tanaman Hortikultura dan Perkebunan di Kawasan Agrowisata Desa Kerta Kecamatan Payangan Kabupaten Gianyar REZA ADELIA; I NYOMAN DIBIA; I MADE MEGA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.4, Oktober 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Land Suitability Evaluation of Horticultureand Plantation Commodities in the Kerta’s Village Payangan Subdistrict Gianyar District Judging from their natural conditions Kerta’s Village Payangan Subdistrictpotential in developing horticultural crops (pineapple, banana, orange, and bark,) and plantations (robusta coffee). The purpose of this study are (1) determine the suitability of land several commodities (pineapple, banana, orange, and bark) and plantations (robusta coffee), (2) determine the limiting factor horticultural commodities (pineapple, banana, oranges, and bark) and plantations ( robusta coffee), (3) create a map of the land suitability of horticultural commodities (pineapple, bananas, oranges, and bark) and plantations (robusta coffee). This study uses a survey of the land suitability evaluation by Djaenudin and others (2003). Based on the results of delineation, there are 6 units of homogeneous land. Actual Land Suitability horticultural crops (pineapple, banana, orange, and bark,) ranged from S2, S3 and N while the suitability of potential land to plant pineapples, oranges, and the bark is on land units IVHPKC, VHPKC, IVHPTG, VHPTG is S1 and to plant banana on land is S2. On land units VIHPKC and VIHPTG for banana plants belonging to N by a factor limiting the slopes. For Robusta coffee classified as S1 on all land units except on land VIHPKC and VIHPTG.
Kesesuaian Lahan Tanaman Buah-buahan dan Perkebunan Berbasis Sistem Informasi Geografis Pada Lahan Kering di DAS Yeh Ho Kabupaten Tabanan CHRIST ALVIN TIMOTHY SIREGAR; NI MADE TRIGUNASIH; I NYOMAN DIBIA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.3, Juli 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Land Suitability For Fruits Plants And Plantations Based On Geographic Information Systems On Dry Land In Yeh Ho Watershed, Tabanan Regency This research aims to discover the actual and potential of land suitability, limited factor, improvement effort, land use management, and the creation of land suitability maps and direction of use land for fruit plants and plantations. Plants that are evaluated include pomelo, durian, banana, passion fruit, vanilla, pepper, robusta coffee, arabika coffee, coconut and cocoa. The research was conducted in Yeh Ho Watershed Tabanan Regency from January 2020 to March 2020. The research method uses the land evaluation survey method. Land suitability assessment is carried out to the sub-class level, by matching the quality of the land with the growing requirements of the plants evaluated. The results showed that classes of actual land suitability for fruits plants and plantations are S1 (very suitable) until N (not suitable). The limited factors in the research area include: temperature, rainfall, dry month, humidity, texture, coarse material, soil depth, pH, salinity, slope, erosion hazard, surface rock, and the rock outcrops. Improvement of the land-suitability can be done with improvement effort except temperature, rainfall, humidity, coral material, surface rock, and rock outcrops because they are permanent barrier factor. Potential suitability class of land for some fruit plants and plantations evaluated are S1 (highly suitable) until S3 (marginal suitable), with limited factors among others temperature, rainfall, humidity, coarse material, surface rock, and rock outcrops factors. Land use recommendations on LCKIVKc and ACKIVTg for robusta coffee plants; ACKIVKc for pomelo plants; LCKIIKc for durian, banana, pomelo, coconut and pepper plants; LCKIIITg for pepper and vanilla plants; LCKIIIKc for pomelo and banana plants.
Evaluasi Kesesuaian Lahan Beberapa Komoditas Tanaman Holtikultura Berbasis Sistem Informasi Geografis di Kawasan Subak Kecamatan Denpasar Barat I KADEK ANGGA WIRA SENTANA; I NYOMAN DIBIA; NI MADE TRIGUNASIH
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Land Suitability Evaluation of Several Horticultural Crop Commodities Based on Geographic Information Systems in the Subak Area of West Denpasar This study aims to determine the actual and potential land suitability, limiting factors, assumptions of improvement efforts, making land suitability maps, and land use direction maps for vegetable and flower horticultural crops. The plants evaluated included spinach, mustard greens, eggplant, marygold, and sunflowers. This research was conducted in West Denpasar from December 2020 to March 2021. Based on the distribution of subaks in West Denpasar, there are eight subaks. The research method used survey methods to evaluate land suitability, soil analysis, and use matching methods between land quality/characteristics and plant growth requirements evaluated by Ritung et al., (2011). The results showed that the actual land suitability classes for vegetable and flower horticultural crops were classified as S1 (very suitable) to S3 (according to marginal). Land characteristics that act as limiting factors include temperature, drainage, texture, N-Total, and K2O. Improvement efforts can be made by adding fertilizers and constructing a drainage system channel, except for the limiting factor of temperature and texture, which are permanent limiting factors. The potential land suitability classes for vegetable and flower horticultural crops evaluated were S1 (very suitable) to S2 (quite suitable), with a temperature limiting factor. Based on land suitability and limiting factors for land use recommendations that can be developed in West Denpasar, namely eggplant, marygold, and sunflower, horticultural crops have more priority than spinach and greens mustard.
Aplikasi Sistem Informasi Geografi (SIG) untuk Kajian Fluktuasi Muka Air Tanah dan Karakteristik Akuifer di Kecamatan Denpasar Utara, Kota Denpasar I GUSTI AYU ADE TEJASTINI; I WAYAN DIARA; I NYOMAN DIBIA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.2, April 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Aplication Geographic Informastion System (GIS) for Study of Ground Water Fluctuations and Characteristics of Aquifers in the District of North Denpasar, Denpasar City The use of water in everyday life is very influential on the pattern of water use available. Ground water is water located on rock is layer below the water is surface. The study was conducted in North Denpasar District, Denpasar City from November 2018 to March 2019. The method used is a survey method, purposive sampling method and descriptive quantitative. The results of the study is ground water fluctuations are ??between 0 m - 5 m and 5 m - 10 m. Distribution of the depth of the ground water in the North Denpasar District which varied greatly in the dry season between 4,5 m – 22,1 m and in the rainy season between 5,1 m – 21,6 m. Ground water fluctuations in the study area are ??between 0 m - 5 m and 5 m - 10 m. The direction of ground water flow in North Denpasar District is predicted flowing to west, south west and to south but leaning more to the southwest. Characteristics of aquifers in North Denpasar District is classified as good aquifers with rock constituent material consisting of sand tuff, sandstone and clay sand which can potentially store and drain water.
Produksi dan Mutu Jagung Manis (Zea mays saccharata Sturt.) Akibat Pemupukan Kimia, Organik, Mineral, dan Kombinasinya pada Tanah Inceptisol Kebun Percobaan Fakultas Pertanian Universitas Udayana MALINDA NOVIARINI; NENGAH NETERA SUBADIYASA; I NYOMAN DIBIA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.4, Oktober 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The Impact of Chemical Fertilization, Organic, Mineral, and The Combination of Fertilizer towards the Production Number and Quality of Sweet Corn (Zea mays saccharata Sturt.) in Inceptisol Experimental Garden Agriculture Faculty of Udayana University The study aims to figuring out the used of some fertilizers in order to increase the production and the quality of sweet corn in Inceptisols area, and also for figuring out chemicals characteristic and soil fertility in Pegok Village, Denpasar. This research was conducted on August until November 2016, the research took place in the Experimental Garden Faculty of Agriculture, and Soil Laboratory and Environmental Sciences, Faculty of Agriculture, Udayana University. The design method was used for this research was a randomized complete block resign (RCBD) using seven treatments and three-time repetition with the number of plots 21 consist of P0 = Control, P1 = Organic fertilizer, P2 = Mineral fertilizer, P3 = Chemical fertilizer, P4 = Combination Fertilizer (PO + PK), P5 = Combination Fertilizer (PM + PK), P6 = Combination Fertilizer (PO + PM + PK). When the result of variance statistical test shows different result, the procedure followed by LSD method (Least Significance Different) with 95% of confidence level using Costat program. The result of statistical analysis shows the role of chemical fertilizers was proven ability to increase the sweet corn production number, the highest result was 13,97 tons ha-1 which use the P3 treatment (200 kg Phonska and 200 kg Urea) ha-1 increased 110,08%, it showed a better result compared to P0 (Control) which only showing 6,65 tons ha-1. The best quality of sweet corn was shown by P6 formula: the combination of organic, chemical and mineral fertilizer.
Analisis Daya Dukung Air Tanah untuk Kebutuhan Dometik dan Pariwisata di Kota Denpasar NOVIN JUWITA CAHYANI; I NYOMAN DIBIA; NI MADE TRIGUNASIH
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.1, Januari 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Analysis of the Carrying of Groundwater for Domestic and Tourism Needs in the Denpasar City This research aimed to know the carrying capacity of groundwater for domestic needs and tourism (hotel) in the Denpasar city. This research was held on November 2016 until April 2017. Primary data collection techniques was done with interviews to the people who directly need water for domestic and non domestic purposes. Determination of the respondents was conducted by purposive sampling. The number of respondents that were interviewed in this study was 100 respondents. The percentage of the population whose water needs are fulfiled from the local water company is as much as 38%, from the bore well is as much as 44% and from the dig well is as much as 18%. Most of the drinking water needs of the community in the Denpasar city use bottled water.Groundwater potential of Denpasar city based on run off coefficient calculation and groundwater basin were 13.75 million m3/year and 52.35 million m3/year. Water needs for domestic purpose of Denpasar city was 65,469,173 m3/year, while the non domestic purpose was 1,482,410.87 m3/year. Comparison between the availability of water by domestic and non domestic needs (hotel) community of Denpasar city, based on the results of the calculation of an index of carrying capacity water is. 0.9, which means the Denpasar city was included in the area that water deficit.
Evaluasi Kesesuaian Lahan Untuk Tanaman Padi dan Palawija pada Lahan Sawah di Kecamatan Sawan Kabupaten Buleleng Berbasis Sistem Informasi Geografis MESI BR HALOHO; I NYOMAN DIBIA; NI MADE TRIGUNASIH
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.2, April 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Evaluation of Land Suitability for Paddy and Secondary Crops on Rice Fields in Sawan District, Buleleng Regency, Based on Geographical Information System This study aims to determine the actual and potential of land suitability, limiting factors, assumptions of improvement efforts, land use management, and making land suitability maps and land use direction maps for rice and secondary crops. The plants evaluated in this study included rice, soybeans, green beans, peanuts, and maize. This research was conducted in Sawan District, Buleleng Regency, from July 2020 to October 2020. Based on the intercropping of soil types map and the slope, seven homogeneous land units were obtained. The research method used was a land evaluation survey, analysis, and land suitability classification. It is based on the criteria for plant growth requirements, according to Ritung et al. (2011), which were matched to the quality/characteristics of the land. The results research show that the actual land suitability classes for rice and secondary crops are from S1 (very suitable) to S3 (suitable to marginal). The limiting factors in the research area include temperature, rainfall, dry months, humidity, drainage, texture, coarse materials, P2O5, K2O, and slopes. Improvement of land suitability classes can be carried out through several factors, except for temperature, humidity, coarse materials, surface rock, and rock outcrops because they are permanent limiting factors. Improvement efforts for limiting factors that can be improved are repairing the drainage system, fertilizing, adding organic matter, mulching, and making terraces. The potential land suitability classes for rice and secondary crops that are evacuated are from S1 (very suitable) to S3 (suitable marginal) with the limiting factors for temperature, rainfall, humidity, coarse material, surface rock, and rock outcrops. The recommendations for land use in SLH RCISw, RCKISw, LCKIISw, RCKIISw, and LCKIIISw are rice, soybeans, and green beans. RCKIIISw and RCKIVSw are soybeans, peanuts, and maize.
Pengaruh Komposisi Media Tanam terhadap Pertumbuhan dan Hasil Tanaman Pakcoy (Brassica chinensis L.) KARINA SAFITRI; I PUTU DHARMA; I NYOMAN DIBIA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.4, Oktober 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Effect of Plant Media Composition on Growth and Yields of Pakcoy (Brassica chinensis L.) The high rate of population growth in Indonesia has had a significant impact on total consumption and food needs. The increasing number of population also increases the need for food, however the high demand for food has not been supported by the relatively low level of food production. Planting in polybags is an alternative that can be developed in urban communities by utilizing as little land as possible to continue to be able to carry out cultivation activities so that an urban household is able to meet its own food needs. Pakcoy is one of type of vegetable that has commercial value and is popular with the public because it tastes delicious, crispy, and fresh. The potential for production and market demand for this vegetable is very large, so it needs to be utilized and managed properly and environmentally friendly, but the planting medium often occurs and plants often die due to the composition of the planting medium that does not match the plant's needs. This study aims to obtain the composition of the planting media that can provide the highest yields on the growth of Pakcoy. This study used a randomized block design (RAK) with 7 treatments for the composition of the planting media and 4 replications. The treatment level consists of KA0 (100% soil), KA1 (90% soil + 5% compost + 5% charcoal), KA2 (80% soil + 10% compost + 10% charcoal), KA3 (70% soil + 20% compost + 10% charcoal), KA4 (60% soil + 30% compost + 10% charcoal), KA5 (50% soil + 30% compost + 20% charcoal), KA6 (40% soil + 40% compost + 20 % charcoal). The results showed that the composition of the planting media significantly affected plant height and number of leaves age 21, leaf area, stem diameter, fresh weight total of the plant and dry weight total of the plant. The KA6 media treatment (40% soil + 40% compost + 20% charcoal) reaches 13.60% increase in plant height at age 21, number of leaves age 21 (14,28%), leaf area (59,74%), stem diameter (16,08%), fresh weight total (50%), dry weight total (89,56%) compared to KA0 (100% soil).
Co-Authors Abd. Rahman As-syakur ALDIYO BARUS Anak Agung Gde Putra Bambang Sumiarto CHRIST ALVIN TIMOTHY SIREGAR Deni Suwardhi Gusti Ayu Yuniati Kencana Hapsari Mahatmi Helen Scott-Orr Heru Susetya I DEWA MADE ARTHAGAMA I Gede Oka Darsana I GEDE PADMANABHA I GUSTI AYU ADE TEJASTINI I Gusti Ngurah Kade Mahardika I GUSTI PUTU RATNA ADI I KADEK ANGGA WIRA SENTANA I Ketut Puja I MADE ADI SURYADI I Made Mega I Nengah Netera Subadiyasa I NYOMAN MERIT I NYOMAN RAI I PUTU DHARMA I Wayan Dana Atmaja I Wayan Diara I WAYAN NARKA I Wayan Nuarsa I Wayan Rusna I Wayan Suarna Ida Bagus Suryawan IGNA Wisnu Adi Saputra INDAYATI LANYA Josua Sintong Martin Hutauruk KARINA SAFITRI Ketut Wikantika Made Suci MALINDA NOVIARINI MALINDA NOVIARINI MESI BR HALOHO NI KADEK DIAN ASRI LESTARI Ni Luh Wayan Sayang Telagawathi NI MADE TRIGUNASIH Ni Nengah Soniari Ni Wayan Intan Martinez NOVIN JUWITA CAHYANI NYOMAN SEMADI ANTARA Putu Dimas Abiyoga R. Suyarto REZA ADELIA RIAN REHMAMANA RIO PUTRA NUSANTARA PARDEDE SHERLEYTA PARIPURNANI Wayan Redi Aryanta WIYANTI WIYANTI Yoga Yuniadi