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All Journal Buletin Peternakan Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Annales Bogorienses Berita Biologi
Atit Kanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Journal : ANNALES BOGORIENSES

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Cellulolytic Yeast Isolated From Raja Ampat Indonesia Kanti, Atit; Sukarno, Nampiah; Sukara, Endang; Darusman, Latifah K
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.042 KB) | DOI: 10.1234/46

Abstract

The objective of this study was to select and characterize three yeast isolates originating from soil of  Raja Ampat region of Papua, Indonesia for its potential to produce cellulase . Selection and characterization of cellulolytic yeast was carried out by measuring cellulolytic Index (IS) with congo red method and measurement of Carboxy Methyl Cellulase (CMC-ase) activity through determination of reducing sugar with dinitrosalycilic methods. Cellulolytic Index (IS) of the isolates  Sporobolomyces poonsookiae Y08RA07,  Rhodosporidium paludigenum Y08RA29 and Cryptococcus flavescens Y08RA33were 1.40, 2.60 and 1.66 respectively. CMC-ase produced optimum at pH 8 at 37ºC by isolate Y08RA07, whereas for Y08RA29 andY08RA33 were at pH 6, at 28ºC. Paper waste was good substrate for cellulase enzyme production by isolate Y08RA07, while for two other isolates the best substrate was CMC. Isolate Y08RA29 having highest cellulase activities when grown in CMC, while isolates Y08RA07 and Y08RA33 achieved highest enzyme activity when grown in bamboo leaf.   Key words: Cellulolytic yeast, Raja Ampat, waste paper, bamboo leaf
Production of Phytase, Amylase and Cellulase by Aspergillus niger, Neurospora crassa, and Rhizophus oryzae on Sargassum and Rice Bran Under Solid State Fermentation Kanti, Atit
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (949.036 KB) | DOI: 10.14203/ab.v19i2.252

Abstract

The objective of study was to produce amylase, cellulase and phytase on sargassum and rice bran on solid state fermentation using Aspergillus niger, Neurospora crassa and Rhizophus oryzae. Media for solid state fermentations composed of dried sargassum and rice bran. The effect of particle of sargassum, initial moisture content on phytase, amilase, and cellulase were evaluated. Optimum enzyme activity of phytase, amylase and cellulase were obtained after 4 days fermentation at 30°C, and initial moisture content was adjusted to 60%. The optimum particle size of dried sargassum attaining the highest enzyme activity was 25 mesh. Best formula forenzymes production was at the ratio of 4:6 (w/w) of Sargassum spinosum (SS) and rice bran (RB) respectively. At this formula highest phytase activity was obtained by Aspergillus niger, cellulase by Rhizopus oryzae, and amylase by Neurospora crassa. Media composed of sargassum and rice bran can be used for phytase, amylase and cellulase production.
Co-Authors Ahmad Thontowi Ahmad Thontowi ANDO KATSUHIKO ANDRIA AGUSTA Anja Meryandini Ariyanti Oetari Arwan Sugiharto Bambang Prasetya Chandra, Nicholas Dwi CHIHARU NAKASHIMA Elsera Br Tarigan, Elsera Br Endang Kusdiyantini Endang Sukara Euis HERMIATI Evana Faradila Ayu, Near Putri Guswenrivo, Ikhsan Habibi, Moh Hafid, Lukman Heddy Julistiono HEDDY JULISTIONO Helbert Helbert I Made Sudiana I Made Sudiana Idris Idris Ikhwani, Azra Zahrah Nadirah Kamal, Andi Saptaji Kartika Dyah Palupi latifah K Darusman Latifah K. Darusman Latupapua, H.J.D. LATUPAPUA, H.J.D. Lestari, Agis Dwi lina marlina, lina Lutfi Nia Kholida Maman Rahmansyah Mia Kusmiati Miftahul Ilmi, Miftahul Muhamad Ilyas Muhamad Ilyas, Muhamad Muhammad Ilyas Mujahidah, Shofia Mujahidah, Shofia N Nurhayati Nampiah Sukarno Napitupulu, Toga P. Napitupulu, Toga Pangihitan Napitupulu, Toga Pangihotan Pandanwangi, Aisyah Amelia Pratama, Aulia Brellian Purbani, Debora Christin Putri, Ade Lia RASTI SARASWATI Raunsai, Marlin Megalestin Rosida Rosida Senlie Octaviana Sri Listiyowati Sudiana, I. Made Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Suprapedi Suprapedi Urip PERWITASARI Wellyzar Sjamsuridzal WIBOWO MANGUNWARDOYO Yantyati Widyastuti Yopi YULIASRI JAMAL Yuliasri Jamal