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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Management of Aquatic Resources Journal (Maquares) Journal of Marine Research Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Perikanan Universitas Gadjah Mada

Sri Sedjati

Departemen Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro Marine Science Techno Park, Universitas Diponegoro

Author-ID : 2957759

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Immunology & microbiology

Published : 54 Documents Claim Missing Document

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Uji Toksisitas Ekstrak Pigmen Kasar Mikroalga Spirulina platensis Dengan Metode Uji Bslt (Brine Shrimp Lethality Test) Rizkina, Rani Agustian; Yudiati, Ervia; Sedjati, Sri
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Spirulina platensis is a microalgae which rich of nutrients and has a potential extracts as an antioxidant, antimicrobial, antiviral, anti-inflamantory and antitumor. The aims of this research were to determine toxicity of methanol extract and crude pigment (methanol-acetone and diethyl ether fractions) of S. platensis against nauplii Artemia sp. aged 24 hour at 3rd instar as an antitumor. The aims of this research were to determine toxicity of methanol extract and crude pigment (methanol-acetone and diethyl ether fractions) of S. platensis against nauplii Artemia sp. The research was conducted in August-October 2011 at Microalgae Laboratory, Marine Station, Diponegoro University Teluk Awur Jepara. Toxicity test methanol and crude pigment extract calculated by LC50-24 hour with BSLT (Brine Shrimp Lethality Test) methodes and pigment analysis was carried out by TLC (Thin Layer Chromatography). The results of BSLT (Brine Shrimp Lethality Test) methanol extracts and crude pigment (methanol-acetone and diethyl ether fractions) showed that LC50-24 hour values of each extract methanol (446,68 ppm), crude pigment extract ethanol-acetone (134.9 ppm) and a crude fraction pigment extract diethyl ether (91.2 ppm). The crude pigment has a cytotoxic effect LC50-24 hours <1000 ppm showed that the extracts are toxic to nauplii Artemia sp. 24 hours, 3rd instar and has a potential antitumor compounds. The results of pigments identification showed contains carotenoids and chlorophylla.

Pengaruh Suhu Ekstraksi Terhadap Kualitas Alginat Rumput Laut Turbinaria sp. dari Pantai Krakal, Gunung Kidul-Yogyakarta Wibowo, Arvianto; Ridlo, Ali; Sedjati, Sri
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Turbinaria sp. is a type of brown algae that produce alginate. Alginate is widely used in industry, such as for a coagulant, suspending, stabilizers, film formers, gel formation, and emulsifier. The objective of research was to know the effect of temperature exstraction toward quality of alginate such as yield, water content, ashes content, and viscosity of Turbinaria sp. Sampling of Turbinaria sp. was done in Krakal Beach Gunung Kidul, Yogyakarta. The method used was experimental method. Design experiment applied was Complete Randomized Design (CRD) with Na2CO3 7 % by different Temperature treatment from 40 ÂºC 50 ÂºC, 60 ÂºC, 70 ÂºC, and 80 ÂºC of concentration triplicate toward alginate quality. The results showed that the higher temperature exstraction caused higher yield, and lower water content, ashes content, and viscosity. The result showed the highest yield was obtained at temperature 80 ÂºC (27,96 Â± 0,34 %). The lowest water content was obtained at temperature 80 ÂºC (15,92 Â± 0,63 %). The lowest ashes content was obtained at temperature 80 ÂºC (23,28 Â± 2,51 %). The highest viscosity content was obtained at temperature 40 ÂºC (26,81 Â± 1,20 cPs). Treatment temperature 50 ÂºC produce temperature best if seen from the odor of which set standard.

Antibacterial Activities of the Extracts of Sponge-Associated Fungus Trichoderma longibrachiatum against Pathogenic Bacteria Sedjati, Sri; Ambariyanto, Ambariyanto; Trianto, Agus; Supriyantini, Endang; Ridlo, Ali; Bahry, Muhammad Syaifudien; Wismayanti, Gita; Radjasa, ocky Karna; McCauley, Erin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 2 (2020): August 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i2.438

This study aims to explore the antibacterial potential of a sponge-associated fungus Trichoderma longibrachiatum isolated from Ternate waters, North Maluku, Eastern Indonesia. Various culture media were used to stimulate the production of secondary metabolites in T. longibrachiatum. The isolate was cultured in various media for 6-9 days. Then, the antibacterial activities of the ethyl acetate extracts were assayed against pathogenic bacteria of Multi-Drug Resistant (MDR) strain (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus cereus). The results showed that all extracts had similar profiles on the thin layer chromatography. However, two of the most potent extracts were produced from the PCA and MEA media for 9 days. These extracts inhibited methicillin-resistant S. aureus (MRSA) (12.48 mm and 12.27 mm); B. cereus (12.11 mm and 12.12 mm); K. pneumoniae (12.40 mm and 10.76 mm); and P. aeruginosa (11.59 mm and 8.69 mm) at concentrations 500 mg/disc. In conclusion, the fungus T. longibrachiatum that was cultured in PCA and MEA media had the potential to produce antibacterial compounds against MDR pathogens and both had similar compounds.Â Meanwhile, theÂ ethyl acetate extracts from fungus cultured in the TPA and TA media were inactive against all tested bacteria

Profil Pigmen Polar dan Non Polar Mikroalga Laut Spirulina sp. dan Potensinya sebagai Pewarna Alami (Profile of Polar and Non-Polar Pigment from Marine Microalgae Spirulina sp. and Their Potential as Natural Coloring) Sri Sedjati; Ervia Yudiati; Suryono Suryono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 3 (2012): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian pigmen Spirulina sp. dilakukan untuk menentukan kandungan pigmen polar dan non polar dari Spirulina sp. dengan metode Spektroskopi UV-Vis. Pigmen polar diekstraksi dengan menggunakan larutan buffer fosfat (NaOH-KH2PO4) pH 7, sedangkan pigmen non polar diekstraksi dengan menggunakan aseton murni. Hasil penelitian menunjukkan bahwa pigmen polar berwarna biru dan kadarnya adalah sebesar 42,272±0,05 mg/g (berat kering), pigmen ini terdiri dari fikosianin (29,159±0,29 mg/g), allofikosianin (9,363±0,20 mg/g), dan fikoeritrin (3,750±0,09 mg/g). Warna pigmen non polar adalah hijau terang dan kadarnya sebesar 4,498±0,06 mg/g (berat kering), pigmen ini terdiri dari klorofil a (3,349±0,03 mg/g) dan karotenoids (1,158±0,03 mg/g). Ekstrak pigmen polar Spirulina sp. kadarnya lebih tinggi dibanding pigmen non polarnya. Pigmen fikosianin berpotensi dikembangkan sebagai bahan aditif pewarna biru alami, karena: kadarnya paling tinggi, menghasilkan warna biru cerah dan cemerlang, bersifat nutrisi fungsional dan tidak beracun.Kata kunci: Spirulina sp., polar, non polar, pigmen, alamiResearch on pigments from Spirulina sp. powder has been done in order to determine polar and non polar pigments content by UV-Vis Spectroscopy method. Polar pigments were extracted using phosphate buffer (NaOH-KH2PO4) pH 7, while non polar pigments were extracted using pure aseton. The result showed that colour of polar pigment was dark blue and its content was 42,272±0,05 mg/g (dry weight), these pigments consist of phycocyanin (29,159±0,29 mg/g), allophycocyanin (9,363±0,20 mg/g), and phycoerythrin (3,750±0,09 mg/g). The colour of non polar pigment was light green and its content was 4,498±0,06 mg/g (dry weight), these pigments consist of chlorophyll a (3,349±0,03 mg/g) and carotenoids (1,158±0,03 mg/g). Due to the high content of pigments from Spirulina sp, showing a bright blue colour, having functional nutrition, and non toxic, the polar pigment phycocyanin have been found suitable for use as additif natural blue colorant.Key word: Spirulina sp., polar, non polar, pigment, natural

Pertumbuhan dan Kandungan Lutein Dunaliella salina pada Salinitas yang Berbeda Faith Dibri Kimberly; Endang Supriyantini; Sri Sedjati
Buletin Oseanografi Marina Vol 8, No 1 (2019): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Dunaliella salina adalah salah satu mikroalga yang mengandung pigmen lutein. Lutein memiliki manfaat sebagai antioksidan untuk melawan radikal bebas pada mata. Pertumbuhan mikroalga dipengaruhi oleh berbagai macam faktor lingkungan, salah satunya adalah salinitas. Penelitian ini bertujuan untuk menentukan salinitas terbaik guna mengoptimalkan pertumbuhan dan produksi pigmen lutein pada D. salina. Metode yang digunakan adalah eksperimen laboratoris. Mikroalga D. salina dikultivasi dengan lima perlakuan salinitas yang berbeda yaitu 20, 25, 30, 35, dan 40 ppt. Pertumbuhan sel D. salina diamati selama 9 x 24 jam kemudian dipanen untuk perhitungan biomassanya. Biomassa basah hasil kultivasi diekstraksi menggunakan pelarut aseton. Ekstrak aseton D. salina kemudian dianalisis kandungan pigmen luteinnya secara spektrofotometrik. Hasil penelitian menunjukkan bahwa perlakuan salinitas berpengaruh secara signifikan terhadap pertumbuhan sel dan kandungan pigmen lutein D. salina. Pertumbuhan sel D. salina optimum pada perlakuan salinitas 30 ppt,yaitu sebesar 125,86 x 104 sel/mL, sedangkan untuk kandungan pigmen lutein D. salina diproduksi optimum pada salinitas 25 ppt, yaitu sebesar 0,0077 µg/g. Dunaliella salina is a microalga containing lutein pigment. Lutein has the role of being an antioxidant to fight free radicals in the eye. Microalgae growth is influenced by a variety of environmental factors, such as salinity. The purpose of this research is to determine the best salinity to optimize the growth and production of lutein pigments in D. salina. The method used in this research was a laboratory experiment. Microalgae D.salina was cultivated with five different salinity treatments, which 20, 25, 30, 35, and 40 ppt. Growth of D. salina cells was observed for 9 x 24 hours and then harvested for the biomass determination. The wet biomass from the cultivation results was extracted using acetone solvent. D. salina acetone extract was then analyzed for its lutein pigment content spectrophotometrically. The results showed that salinity treatment had a significant effect on cell growth and pigment content of lutein D. salina. The optimum growth of D. salina cell is optimally achieved in 30 ppt salinity treatment at the amount of 125,86 x 104 cell/mL, while for the lutein pigment content of D. salina is optimally achieved in 25 ppt salinity at the amount of 0,0077 µg/g.

Komposisi Dan Kelimpahan Fitoplankton Di Laguna Segara Anakan, Cilacap Budhy Wiyarsih; Hadi Endrawati; Sri Sedjati
Buletin Oseanografi Marina Vol 8, No 1 (2019): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Segara Anakan merupakan laguna yang terletak di selatan Pulau Jawa tepatnya di perbatasan antara Jawa Barat dengan Jawa Tengah. Kawasan Segara Anakan merupakan tempat bertemunya sungai besar, sehingga mengalami perubahan penurunan kualitas perairan berupa penyempitan dan pedangkalan akibat proses sedimentasi yang tinggi. Hal ini dikhawatirkan dapat mengakibatkan menurunnya kualitas perairan yang dapat mengganggu pertumbuhan fitoplankton di perairan. Fitoplankton merupakan parameter biologi yang dapat dijadikan bioindikator untuk mengevaluasi kualitas dan tingkat kesuburan suatu perairan. Kondisi tentang lingkungan perairan diperkirakan berubah sepanjang waktu, maka perlu penelitian fitoplankton, untuk mengetahui tingkat kesuburan perairannya. Tujuan dari penelitian ini adalah untuk mengetahui komposisi dan kelimpahan fitoplankton di Laguna Segara Anakan, Cilacap. Penelitian ini menggunakan metode deskriptif eksploratif dengan pengambilan data menggunakan purposive sampling method. Hasil menunjukkan ditemukan 34 genera yaitu Kelas Bacillariophyceae (21 genera), Kelas Cyanophyceae (4 genera), Dinophyceae (6 genera), Chlorophyceae (2 genera), dan Euglenophyceae (1 genus). Kelimpahan fitoplankton berkisar antara 12.945,8-38.194,1 sel/liter. Indeks Keanekaragaman termasuk kategori sedang dengan nilai berkisar 1,60 - 2,32. Indeks keseragaman termasuk kategori sedang hingga tinggi, dengan nilai berkisar 0,59 - 0,81. Indeks dominansi berkisar antara 0,18 - 0,44. menunjukkan bahwa tidak ada genus yang mendominasi. Segara Anakan is a lagoon located in the south of Java Island precisely on the border between West Java and Central Java. Segara Anakan area is the place where the big river meets. Water quality decrease because of narrowing the lagoon due to the high sedimentation process. This may affect the presence of phytoplankton in the water. The quality and the fertility level of aquatic can be evaluated through the phytoplankton existence since phytoplankton is used as a biological indicator for the marine environment. The condition of the aquatic environment is expected to change over time, so it is necessary to study phytoplankton. The purpose of this research is to know about the composition and abundance of phytoplankton in Segara Anakan Lagoon, Cilacap. This research used the descriptive explorative method by data sampling using purposive sampling method. The study showed 34 genera of Bacillariophyceae (21 genera), Cyanophyceae (4 genera), Dinophyceae (6 genera), Chlorophyceae (2 genera), and Euglenophyceae (1 genus). The abundance of phytoplankton ranging from 12.945,8-38.194,1 cells/liter. The Diversity Index is a medium category with values ranging from 1.60 to 2.32. The evenness index is moderate to high, with values ranging from 0.59 to 0.81. The Dominant index ranges from 0.18 to 0.44. The value indicates the category of no dominating genus.

Komposisi Larva Ikan Pada Tutupan Padang Lamun di Perairan Prawean Bandengan, Kabupaten Jepara Sri Redjeki; Riska Novianti Putri; Adi Santoso; Sunaryo Sunaryo; Sri Sedjati
Buletin Oseanografi Marina Vol 8, No 2 (2019): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Larva Ikan (ichtyoplankton) merupakan tahapan awal dari daur hidup ikan dimulai dari perkembangan telur, larva dan juvenil, memiliki tingkat mortalitas tinggi dan peka terhadap perubahan lingkungan, predator, dan kesediaan makanan. Fungsi ekologis padang lamun sebagai daerah asuhan dan tempat berlindung bagi semua jenis organisme laut kecil, salah satunya larva ikan. Kerapatan atau tutupan padang lamun juga sebagai salah satu faktor pendukung melimpahnya organisme dan kekayaan di laut. Tujuan Penelitian ini untuk mengetahui kelimpahan dan distribusi larva ikan yang terdapat pada ekosistem padang lamun, serta mengetahui hubungan kelimpahan larva ikan dengan tutupan padang lamun di Perairan Prawean Bandengan, Jepara. Metode penelitian ini adalah metode deskriptif dengan penentuan lokasi sampling menggunakan purposive sampling methode. Lokasi penelitian pada 3 stasiun dengan pembagian kerapatan lamun yang berbeda (I = Padat ; II = Sedang ; III = Jarang) dan dilakukan sebanyak 3 kali pengulangan sampling di masing-masing lokasi. Pengambilan sampel larva ikan dilakukan dengan menggunakan larva net (P = 0,9 m ; L : 0,6m) dengan ukuran mata jaring 800 µm. Hasil penelitian ini ditemukan larva ikan sebanyak 5 famili yaitu Nemipteridae, Gerreidae, Gobiidae, Labridae, dan Mullidae. Famili larva ikan yang paling sering ditemukan adalah Nemipteridae. Rata-rata kelimpahan famili larva ikan pada Stasiun I sebesar 0,419 ind/m3, Stasiun II sebesar 0,205 ind/m3, dan pada stasiun III sebesar 0,069 ind/m3. Nilai rata - rata indeks keanekaragaman termasuk dalam kategori rendah sedang (0,65–1,37), indeks keseragaman larva ikan termasuk dalam kategori rendah-tinggi (0,33-0,65) indeks dominasi larva ikan menunjukan ada yang mendominasi pada tiga stasiun (0,28–0,30) dan indeks sebaran morisita yang dilakukan menunjukan bahwa sebaran larva ikan pada tiga stasiun merata. Fish larvae (ichtyoplankton) are the initial stages of the fish's life cycle starting from the development of eggs, larvae and juveniles, which have a high mortality rate and are sensitive to environmental changes, predators, and food availability. The ecological function of seagrass beds as nurseries and shelter for all types of small marine organisms, one of which is fish larvae. The density or cover of seagrass beds is also one of the supporting factors for the abundance of organisms and wealth in the sea. The purpose of this study was to determine the abundance and distribution of fish larvae found in the seagrass ecosystems, and to determine the relationship of abundance of fish larvae with cover seagrass beds in the waters of Prawean Bandengan, Jepara. This research method is a descriptive method by determining the sampling location using purposive sampling method. The research location was in 3 stations with a different distribution of seagrass density (I = Dense; II = Medium; III = Rare) and carried out 3 times repetition of sampling at each location. Sampling of fish larvae was carried out using larvae net (P = 0,9 m; L: 0,6m) with a mesh size of 800 μm. The results of this reasearch, found fish larvae of 5 families, namely Nemipteridae, Gerreidae, Gobiidae, Labridae, and Mullidae. The most common family of fish larvae was Nemipteridae. The average abundance of fish larvae at Station I was 0,419 ind/m3, Station II was 0,205 ind/m3, and at Station III was 0,069 ind/m3. The average diversity index was included in the low category (0,65 – 1,37), the uniformity index of fish larvae was included in the low-high category (0,33 – 0,65) the fish larvae dominance index shows that there are dominating at three stations (0,28 – 0,30) and the distribution index of distribution (morisita) conducted showed that the distribution of fish larvae at three stations was evenly distributed.

Analisis Kandungan Agar, Pigmen dan Proksimat Rumput Laut Gracilaria sp. pada Reservoir dan Biofilter Tambak Udang Litopenaeus vannamei Ervia Yudiati; Ali Ridlo; Annisa Afifah Nugroho; Sri Sedjati; Lilik Maslukah
Buletin Oseanografi Marina Vol 9, No 2 (2020): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v9i2.29453

Gracllaria sp. merupakan rumput laut yang memiliki nilai ekonomis tinggi karena menghasilkan agar. Agar banyak dimanfaatkan dalam bidang industri maupun pangan. Kualitas Gracilaria sp. ditentukan oleh kadar agar, pigmen serta proksimatnya. Penelitian ini bertujuan untuk mengkaji pengaruh parameter kualitas media tumbuh Gracilaria sp. terhadap biosintesis agar, klorofil a, karoten dan kadar proksimat (protein, karbohidrat, lemak, abu, dan air). Lokasi pengambilan sampel di reservoir dan biofilter tambak udang Vaname (Litopenaeus vannamei). Kondisi lingkungan perairan yang diukur adalah kandungan nitrat dan fosfat, suhu air, salinitas, DO (oksigen terlarut), serta pH (Derajat keasaman). Ekstraksi agar dilakukan dengan metode alkali. Karakterisasi agar menggunakan analisis Fourier Transform Infra Red. Hasil penelitian menunjukkan kandungan agar (31,4 ± 0,42%) pada reservoir lebih baik dibandingkan biofilter. Begitu pula dengan konsentrasi klorofil a (19,61 ± 0,04 mg/g), karotenoid (7,42 ± 0,21 µmol/g) serta kadar protein (15,38 ± 0,27%). Kadar lemak, karbohidrat, air dan abu pada kedua lokasi tidak berbeda nyata. Hasil karakterisasi agar menujukkan keberadaan gugus galaktosa 3,6-anhydro-L-galaktosa. Gracilaria sp. is well known as to has a high economically value, due to the application of the agar content that is used in various industries especially in food industry. The quality of Gracilaria sp. depends on agar and pigment content as well as proximate analysis. This study aimed to investigate the effect of water quality parameter on Gracilaria sp habitats to agar, chlorophyl a, carotene and proximate analysis (protein, carbohydrate, total lipid, ash, water content). Samples were collected from reservoir and biofilter from Litopenaeus vannamei’s waste pond. Water quality parameter measured were nitrate and phosphate, water temperature, salinity, dissolved oxygen (DO) and pH. Agar extraction was done by alkali methods. Agar and its chemical structure were characterized by FT-IR analysis. Results showed that agar content (31.4 ± 0.42%) on reservoir was better than biofilter. Higher results from reservoir samples were also found in chlorophyl a (19.61 ± 0.04 mg/g), carotenoids (7.42 ± 0.21 µmol/g) and protein level (15.38 ± 0.27 %). On the other hand, total lipid, carbohydrate, water and ash content were similar. Based on FT-IR analysis shows that the galactose 3,6-anhydro-L-galactose was present.

Kandungan Protein Spirulina platensis Pada Media Kultur Dengan Konsentrasi Nitrat (KNO3 ) Yang Berbeda Saniyatul Ulya; Sri Sedjati; Ervia Yudiati
Buletin Oseanografi Marina Vol 7, No 2 (2018): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Spirulina platensis merupakan mikroalga hijau biru yang mengandung nutrisi protein tinggi sehingga banyak digunakan sebagai pakan alami. Pertumbuhan dan kandungan protein mikroalga dipengaruhi oleh beberapa faktor, salah satunya adalah pemberian makronutrien pada media kultur mikroalga. Penelitian ini bertujuan untuk melihat pengaruh pertumbuhan dan kandungan protein pada mikroalga S. platensis dengan pemberian konsentrasi nitrat yang berbeda. Rancangan penelitian yang digunakan adalah rancangan acak blok dengan tiga kali pengulangan. Perlakuan konsentrasi nitrat berbeda yang diberikan adalah 50 ppm, 100 ppm, dan 150 ppm. Perhitungan kepadatan dan pengukuran parameter kualitas air dilakukan setiap hari. Pemanenan dilakukan pada hari ke – empat. Kadar prorein dianalisis dengan menggunakan metode Kjedahl. Hasil penelitian pertumbuhan S. platensis menunjukkan nilai kepadatan sel S. platensis tertinggi pada hari ke – empat berada pada perlakuan C dengan konsentrasi nitrat 150 ppm (169,58 . 103 sel/mm3). Hasil analisis ANOVA menunjukkan bahwa konsentrasi nitrat berpengaruh terhadap pertumbuhan S. platensis (p < 0,05) namun perbedaan konsentrasi nitrat tidak berpengaruh pada kadar protein (p ≥ 0,05). Berdasarkan hasil penelitian dapat disimpulkan bahwa konsentrasi nitrat yang ditambahkan pada media kultur S. platensis berpengaruh terhadap pertumbuhan S. platensis namun tidak berpengaruh pada kandungan proteinnya. Protein Content of Spirulina platensis in Different Culture Media with Nitrate (KNO3) Concentration Spirulina platensis is green-blue microalgae that contain high protein nutrient and could be used as natural food. Growth and protein content of microalgae are influenced by several factors and one of those is giving macronutrient to microalgae’s culture medium. The purpose of this research is to compare the growth and protein content of the S. platensis with different nitrate consentrations.The research design used was a completely randomized block design with three repetitions. The different nitrate concentration treatments were 50 ppm, 100 ppm and 150 ppm. Determination of density and water quality measurement parameters was done on daily basis. Spirulina platensis was harvested done on fourth day of culture. Protein levels were analyzed by Kjedahl method. The result of the S. platensis growth that the highest density on day fourth in C treatment with 150 ppm nitrate consentration (169,58 . 103 sel/mm3). The result of ANOVA analysis show that the concentration of nitrate affected on S. platensis growth (p < 0,05) but the difference of nitrate concentration wasn’t affected in protein analysis (p ≥ 0,05). Based on the result of this research, it can be concluded that the concentration of nitrate added to the S. platensis culture medium effectively and improved the growth of S. platensis but had no effect on the protein content.

Skrining Beberapa Jenis Spons Sebagai Upaya Pencarian Bahan Bioaktif Antijamur Aspergillus flavus dan Candida albicans Aziz Rifai; Irene Ulsadriatny; Lilik Maslukah; Elis Indrayanti; Sri Sedjati; Agus Trianto
Buletin Oseanografi Marina Vol 2, No 4 (2013): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Spons telah diketahui sebagai sumber bahan bioaktif dengan berbagai bioaktivitas seperti antikanker, antivirus, antibakteri dan antijamur. Potensi spons sebagai bahan bioaktif belum banyak diteliti. Penelitian ini bertujuan untuk memperoleh data berbagai jenis spons yang berpotensi sebagai sumber bahan bioaktif anti jamur, maka dilakukan skrining terhadap Aspergillus flavus dan Candida albican. Sampel dikoleksi dari perairan Bandengan dan Empurancak, Jepara dengan SCUBA diving dan skin diving pada bulan April 2003. Sampel selanjutnya diekstrak dengan menggunakan metanol. Uji antijamur dilakukan dengan metoda disk diffusion agar menurut Kirby-Bauer dengan konsentrasi 100, 200 dan 400 µg disk-1. Rendemen ekstrak metanol pada spons berkisar antara 0.04% sampai dengan 7.34% dari berat basahnya. Seluruh ekstrak spons yang digunakan dalam penelitian ini dapat menghambat pertumbuhan C. albicans dan A. flavus pada konsentrasi 200 dan 400 µg disk-1. Ekstrak spons Reniera sp mempunyai bioaktivitas yang tertinggi yang ditunjukan dengan adanya zona hambat sebesar 12.58-14.93 mm terhadap C. albicans dan 6,94 -10,79 mm terhadap A. flavus pada konsentrasi uji. Kata kunci : spons, bahan bioaktif, Aspergillus flavus, Candida albicans, ekstrak

Co-Authors Adam Sojuangon Pasaribu Adella, Alisa Sukma Adha Susanto Adi Santosa Adi Santoso Agus Trianto Ali Ridlo Ambariyanto , Ambariyanto Ambariyanto Amtoni Caesario Nainggolan Annisa Afifah Nugroho Ardiani Widya Wandira Arif Maa’ruf Al Ayyub Arvianto Wibowo Azhar, Riza Nur Aziz Rifai Bahry, Muhammad Syaifudien Bambang Yulianto Bolu, Wa Ode Rima Alam Sari Budhy Wiyarsih Chika Velita Anindya Yulian Deska Dwi Iriana Dewi Meynita, Dewi Dhimas Firmansyaf Dinda Ayuniar Zanjabila Dwi Haryo Ismunarti Eka Mulya Eksa Novritasari Elis Indrayanti Endang Supriyantini Ervia Yudiati Faith Dibri Kimberly Gita Wismayanti Hadi Endrawati Haeruddin Haeruddin Hafida Salma Haqqu, Alir Adn Ighwerb, Mostafa Imhmed Ikhlasul Amal Irene Ulsadriatny Iriana, Deska Dwi Jamaludin Jamaludin Khoirunnisah Riswanti Kirana Fatika Brilianti Larasati, Stefanie Jessica Henny Lilik Maslukah Limbong, Maria Fransiska Linggar Dirgantara Prasetyo M. Hafizulhaq Mahadika Fanindhita Sany Max Rudolf Muskananfola McCauley, Erin Muhammad S. Bahry Muhammad Syaifudien Bahry Nada Kristiani Ginting Nirwani Soenardjo Nur Islamiah Sulastri Nuril Azhar Ocky Karna Radjasa Oetari Kusuma Putri Pasaribu, Adam Sojuangon Pradhika, Vicky Dimas Pramastuti, Fransisca Ria Rabia Alghazeer Rachmat Afriyanto Raden Ario Rani Agustian Rizkina Ria Azizah Tri Nuraini Rima Rosema Rini Pramesti Riska Novianti Putri Risnita Tri Utami Rizky Rifatma Jezzi Rizqi Umi Arifah Sakti Imam Muchlisin Saniyatul Ulya Savitri, Nadiah Humairoh Mufidah Septhy Kusuma Radjasa Sibero, Mada Triandala Sri Redjeki Sri Yulina Wulandari Sulistiana, Zalsabila Sunaryo Sunaryo Sunaryo Sunaryo Suryono Suryono Vicky Dimas Pradhika Victoria Ratna Widiyanti Victorina Yulina Santi Wandira, Ardiani Widya Widiyanti, Victoria Ratna Wilis Ari Setyati Wismayanti, Gita Yusup Bayu Permadi Yusup Bayu Permadi Zidny Nurfadhli

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