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Efek Beta Karoten dan Agregasi Klorofil Pada Fotostabilitas Klorofil a dalam Pelarut Aseton Costa, Junet. F. da; Karwur, Ferry F; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.132 KB) | DOI: 10.31258/jnat.11.2.115-123

Abstract

Chlorophyll as photosynthetic pigment has many benefits to human such as antioxidant, antibacterial, color additive,immunity, and photosensitizer. Concerning to these applications, chlorophyll a will react with oxygen and light andreduce its effectiveness. It is then important to understand the stability of chlorophyll a on oxygen and light.Stability of chlorophyll a in the presence of beta-carotene (1:1) in acetone was studied for various waterconcentrations. Each solution was exposed to red light (ë e” 630 nm) for 0.5; 1.0; 2.5; 5.0; 10; 20; 30; 40; 50; and 60minutes and their spectrum were analysed. When water was added (8.33; 16.67; 25; 33.33; 41.67; 50; 58.33; 66.67;75; and 83.33 percent) to the acetone solution of chlorophyll a, the chlorophylls aggregate, and in the presence ofbeta-carotene, the chlorophyll more stable. The water shifts the chlorophyll a spectrum toward red duringillumination with or without the presence of carotene. The formation of oligomeric chlorophyll a aggregate occurswithin 10-20 minutes after the water was applied. This strategy of aggregating the chlorophylls to dissipateexcess energy captured from light does not support the photostability of chlorophyll as much as beta-carotene.Surprisingly, when beta carotene was mixed with 66.7% and 75.0% of water, the chlorophyll a degraded 5.56% and9.71% respectively. In conclusion, the aggregate form of chlorophyll a and the presence of beta-carotene increasephotostability of chlorophyll a in acetone solution.
Pemisahan dan Identifikasi Isomer Tokotrienol dari Tokotrienol Rich Fraction (TRF) Lodu, Widiyasri; karwur, ferry fredy
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

Palm oil is the largest agricultural product produced by Indonesia. Palm oil contains a large group of vitaminE which are tocopherol and tocotrienol which have been widely reported their efficacy in inhibiting the growthand development of diseases such as antioxidants, anti-cancer, cholesterol inhibitors, prevent premature aging,and cardioprotective effects. This study aims to segregate and identify the intensity of each isomer ofTocotrienol Rich Fraction (TRF). Segregation and identification are done through using columnchromatography, UV-visible spectrophotometer, and HPLC. The spectral pattern of TRF shows the presenceof tocotrienol isomers at wavelengths of 292-298 nm. Five spectra patterns identified with the highest intensitywere demonstrated by the γ-tocotrienol isomers at 10.86 min, δ-tocotrienol at the minute of 9.36, α-tocotrienolat the minute of 12:42, α-tocopherol at 21.11 and α-T1 at 17.35 with 0 intensity.
An Insight Into Maternal Death Caused by Postpartum Hemorrhage in Western Timor, Indonesia Kristiani Desimina Tauho; Ferry Fredy Karwur
Jurnal Keperawatan Indonesia Vol 22, No 1 (2019): March
Publisher : Faculty of Nursing Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7454/jki.v22i1.675

Abstract

Maternal deaths in Timor Island, East Nusa Tenggara Province, are one of the contributors to the overall maternal mortality rate (MMR) of Indonesia. The MMR of Western Timor Island was 150/100,000 live births in 2015. The aim of this qualitative study was to explore the perceived causes of maternal death due to postpartum hemorrhage. Data were obtained from family members, traditional birth attendants, and Posyandu cadres, as well as health providers. Using the retrospective method, this study traced six out of nine postpartum hemorrhage cases in the four sub-districts with the highest maternal death rate in 2010. The research findings showed that most childbirth processes were done at home without any help from health workers. Postpartum hemorrhage happened among women ranging from 24-42 years old; five among them had been pregnant more than four times. Medically, five cases were caused by a prolonged third stage of labor due to a retained placenta. Non-medical factors causing postpartum hemorrhage were poor accessibility, lack of communication devices, and lack of infrastructure.  Keywords: maternal death, postpartum hemorrhage, Western Timor Abstrak Pengetahuan Tentang Kematian Maternal Karena Perdarahan Postpartum di Timor Barat, Indonesia. Kematian maternal di Pulau Timor, Provinsi Nusa Tenggara Timur, merupakan salah satu penyumbang Angka Kematian Ibu (AKI) di Indonesia. Tahun 2015, AKI di Pulau Timor bagian barat adalah sebesar 150/100.000 kelahiran hidup. Tujuan dari studi kualitatif ini adalah untuk mengeksplorasi persepsi para pihak yang paling mengetahui mengenai penyebab kematian maternal karena perdarahan postpartum. Data diberikan oleh anggota keluarga, dukun bersalin, dan kader Posyandu, serta penyedia layanan. Menggunakan metode retrospektif, penelitian ini menyusur enam dari sembilan kasus perdarahan postpartum yang terjadi di empat kecamatan yang memiliki angka kematian maternal tertinggi pada tahun 2010. Hasil penelitian menunjukkan bahwa sebagian besar prosedur persalinan dilakukan di rumah tanpa bantuan dari tenaga kesehatan. Perdarahan postpartum terjadi di antara wanita dengan rentang umur 24–42 tahun; empat di antara mereka sudah pernah hamil sebanyak lebih dari empat kali. Secara medis, lima kasus disebabkan oleh perpanjangan kala tiga persalinan berhubungan dengan retensi plasenta. Faktor- faktor non medis yang menyebabkan perdarahan posrpartum adalah aksesibilitas yang buruk, ketiadaan piranti komunikasi, dan kurangnya infrastruktur. Kata kunci: kematian ibu, perdarahan postpartum, Timor Barat
Evolutionary Perspective of Sulfur Dynamics in Tomohon and Implications on Microbial Corrosion Frity Lisa Taroreh; Jubhar C Mangimbulude; Ferry F Karwur
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2016: Prosiding SNTKK 2016
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Abstract

Sulfur (S) is a non-metal, multivalent, tasteless and odorless element. The vast amount of sulfur valence (from S2- to S6+)allows such element to participate in various geochemical and biochemical processes. In nature, sulfur is everywhere, abundant and distribute evenly on earth and the solar system. On ‘ancient’ earth in anoxic atmospheric condition, sulfur existed in the form of H2S. In oxic period, following the discovery of photosynthesis, sulfur was commonly found in the form of sulfur oxides (SOx). This paper discusses the evolutionary perspective of sulfur by presenting and discussing three following aspects: (i) The formation ofsulfur element through “nucleosynthesis” that generating 4 sulfur isotopes with various abundances (32S= 95.02%; 33S = 0.75%; 34S = 4.21%; 36S= 0.02%), (ii) Geological record of ∆33S and sulphate dynamics in the atmosphere since 2,500,000,000-290,000,000 years ago as volcanic gas; and, (iii) The transformation of various types of sulfur compounds in oxic condition nowadays as SO2 , SO3, H2SO4, sulfite salt, sulfate salt, and organic sulfur aerosols.Special attention is given to the sulfur cycle and changing dynamics in nature related to volcanism in geological context of North Sulawesi, especiallyTomohon areas, and practical implication of microbiological corrosion is considered as the implication of the sulphuric evolution that affect the life of modern society.
Pala (Myristica fragrans Houtt) sebagai bioreaktor hayati senyawa fenilpropanoid: suatu kajian pustaka Angreni B Liunokas; Ferry F Karwur
Jurnal Biologi Udayana Vol 24 No 2 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2020.v24.i02.p05

Abstract

Pala(Myristica fragrans Houtt) sebagai bioreaktor hayati menghasilkan produk utama minyak asiri bernilai ekonomi tinggi, yang tersebar pada bagian-bagian buahnya. Minyak asiri pala tersusun oleh kelompok besar terpenoid dan fenilpropanoid yang memiliki konsentrasi terkecil namun sebagai indikator mutu minyak pala. Konsentrasi minyak pala dilihat dari keberadaan senyawa penyusun ini telah banyak dilaporkan, namun belum sepenuhnya dijelaskan mengenaimetabolisme fenilpropanoid melalui jalur shikimat yang dihasilkan dari fenilalanin, diawali kondensasi aldol antara eritrosa 4-fosfat dan asam fosfoenolpiruvat dengan melibatkan reaksi enzimatik dikomparasi dari beberapa tanaman penghasil fenilpropanoid dan Arabidopsis thaliana sebagai tanaman model, yang menjadi tinjauan tulisan ini. Regulasi biosintesis pembentukan senyawa golongan fenilpropanoid menjadi penting karena kita dapat diarahkan pada prospek pengembangan senyawa fenilpropanoid dan turunannya secara lebih luas dengan meningkatkan produksinya atau dalam kaitannya dengan menghasilkan obat modern yang bernilai jual tinggi. Kata Kunci: Myristica fragrans Houtt, Biosintesis,Fenilpropanoid
Identification of Carotenoids in Halimeda macroloba Reef Associated Bacteria Wiwik Astuti; Ocky Karna Radjasa; Ferry Fredy Karwur; Ferdy S. Rondonuwu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.604 KB) | DOI: 10.14710/ik.ijms.21.4.151-160

Abstract

Several carotenoid pigments which are produced by sea microorganisms are shown to function as antimicrobe compounds and potential antioxidants. Coral reefs with high levels of diverse biota facilitate Halimeda as a primary component and producer to have associated bacteria that produce relatively unknown metabolic compounds. This research attempts to isolate bacteria that are associated with Halimeda macroloba, identify it, as well as analyze the pigment content produced. A yellow-orange bacteria colony is successfully isolated and given the temporary name MK_HM. This bacteria is in the shape of circular (oval) rods and are gram-variable bacteria. Based on the sequencing analytical results, Blast homology, as well as a phylogenetic analysis, it shows that the bacteria have a relationship with Exiguobacterium aestuarii TF-16 at 94%, so that it can be strongly predicted that the MK_HM strain is a new species. This pigment analysis, which is conducted on the Exiguobacterium sp. MK_HM bacteria extract, reveals that this strain produces carotenoid pigments of diadinochrome, semi-α-carotenone, dinoxanthin, and P457. Keywords: Exiguobacterium, carotenoid, diadinochrome, semi-α-carotenone, dinoxanthin, and P457.
Exploration, Isolation, and Identification of Carotenoid from Bacterial Symbiont of Sponge Callyspongia vaginalis Iqna Kamila Abfa; Ocky Karna Radjasa; A B Susanto; Handung Nuryadi; Ferry F. Karwur
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 2 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (776.278 KB) | DOI: 10.14710/ik.ijms.22.2.49-58

Abstract

During the past two decades research on marine bacteria has highlighted the tremendous potential of symbiotic-microorganisms as a source of bioactive secondary. One of the potential of the bacterial symbionts is producing a natural pigment, and these organisms can be used as a sustainable source of natural pigments. Carotenoid is one of the most important pigments that has important roles in physiological and molecular processes of microorganisms, as well as for human health. The objective of this study is to analyze carotenoid pigments from marine bacterial symbionts from sponge and to identify bacterial symbionts that produce carotenoid pigments. Pigment analysis was performed by a UV-VIS spectrophotometer and High Performance Liquid Chromatography (HPLC). Molecular bacterial identification was performed based on 16S rDNA sequence. The isolation of bacterial symbionts from C. vaginalison Zobell 2216E medium resulted in one bacterium, CB-SP5, positively synthesized carotenoids. By reverse phase HPLC analysis, the carotenoid pigments in the bacterial symbionts were identified as diadinoxanthin, fucoxanthin, neoxanthin, dinoxanthin, anddiadinochrome. CB-SP5 shared the highest level of 16S rDNA gene sequence similarity with Psychrobacter celer (99%). Keywords : carotenoid, sponge, bacterial symbiont, 16S rDNA.
Mode of action of tocotrienol as anticancer Widiyasri Lodu; Ferry Fredy Karwur
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 2 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.415 KB) | DOI: 10.19106/JMedSci005102201909

Abstract

Tocotrienols are fat soluble substances members of the vitamin E family with the main properties of antioxidant. They are composed of the chiral center of chromanol ring with polypreniyl side chains. Research suggests that tocotrienols have a number of health benefit, and one of them as an anticancer agent. As an anticancer, tocotrienols could perform in different processes of the multi stages of cancer development and interact with other anticancer medicines and other bioactive substances, suchs geneistein, lovastin, hydroxychavicol, 6- gingerol and sesamin. The anticancer activities occur through their roles as antioxidant, coenzyme, gene expression regulator, and preventing cholesterol synthesis by inhibiting the expression of HMGCR enzyme after transcription, through this method, tocotrienols could control the cholesterol level of cancer cells and prevent the growth of the cancer. Tocotrienols target several signalling pathways at cellular and molecular levels.
Optical Properties and Relative Determination of Caffeine in Black Tea Liquor Monika Rahardjo; Ferry Fredy Karwur; Ferdy Semuel Rondonuwu
Jurnal Sains Dasar Vol 8, No 2 (2019): October 2019
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v8i2.20781

Abstract

Caffeine is one of main quality parameter for teas, known for its ability to increase alertness, and has a slightly bitter flavor. As quality parameter, caffeine content will later determine flavor and aroma profile for teas that affect the price in global market. There were many factors that affected chemical compounds in tea, one of it was growth altitude. This research aimed to characterize caffeine spectra from tea liquor using UV-Vis visible which later can be used to relate caffeine content with the altitude of tea sample plantation. The optical transition of caffeine from brewed tea leaves liquor was measured from a variety of black tea products produced according to quality standards for global market. A total of 63 tea samples from four different grades (FANN, PekoFANN, FANN2, and Dust) and from 12 different tea estates in Indonesia have been replicated in their optical transitions and classified on the basis of caffeine content. It was found that caffeine content in tea products may vary depending on the grade and origin of the tea plantation. FANN grade has higher caffeine content that other grades while products from highland tea plantation generally have more caffeine content for all tea grades.
Analisis Variabilitas Genetik Rigidoporus microporus (Jamur Akar Putih) pada Karet dengan Teknik PCR-RFLP Fragment ITS Ria Novianti; Haryono Semangun; Ferry F. Karwur; Martanto Martosupono
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.023 KB) | DOI: 10.22146/jpti.9383

Abstract

The eleven isolates studied fell into two groups, which showed that the fungus did not have high genetic variability. Isolates from diseased rubber trees in shorter vicinities, which were due to pathogen’s vegetative spread through root contact, have close genetic relationship. Meanwhile isolates from far apart trees, either in one estate or from different islands, does not necessarily have distant genetic relationship. It might be due to the fact that long distance dispersal of the disease could also due to rhizomorph contaminating rubber stumps. PCR-RFLP Fragment ITS Techniques can be applied to study genetic relationship of Rigidoporus microporus isolates. It is expected that the technique will be applied with more isolates from different provinces by using additional different restriction enzymes.