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STUDI KASUS PATOLOGI GIGI: KARIES PADA RANGKA MANUSIA ST1, SONG TERUS, PACITAN, JAWA TIMUR ( Dental Pathology Case Study: Caries On ST1 Human Remains, Song Terus, Pacitan, East Java) Anita Tamu Ina; Dyah Prastiningtyas; Harry Widianto; Florent Détroit; Ferry Fredy Karwur; Andri Purnomo; Anne-Marie Sémah; François Sémah
Jurnal Penelitian Arkeologi Papua dan Papua Barat Vol. 10 No. 2 (2018): November 2018
Publisher : BALAI ARKEOLOGI PAPUA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2137.762 KB) | DOI: 10.24832/papua.v10i2.253

Abstract

Human remains found in Song Terus (Pacitan, East Java), known as ST1, presented an opportunity of in-depth study in reconstructing how human lived during Early Holocene period in the area. This article focuses on palaeopathological aspects by examining lesions of disease observable in bones and dentition of human remains found in archaeological context. The research done for this article focuses more on dental remains, as teeth are known to have durability and longevity as archaeological finds, and could also provide information on age-at-death, types of diet, and oral diseases which may occurred during a person’s life. Dental caries is one of the most common type of oral disease found in archaeological context. Research methods used are macroscopic observation and literature reference comparison.. Results showed there were nine dentition on this individual (from a total of 27 identified dentition) suffered from caries with various degree of severity. Other types of oral disease noted during observation and analysis were periodontal disease. ST1 might have been suffering from severe caries due to lack of oral hygiene, as well as minimum dental treatment towards emerging oral disease. Nevertheless, these diseases did not seem to be directly caused by ST1’s dietary habit during lifetime. ABSTRAK Temuan rangka manusia ST1 di Song Terus (Pacitan, Jawa Timur) memberikan peluang untuk menelusuri lebih jauh pola kehidupan manusia pada periode Holosen Awal di wilayah ini. Artikel ini berfokus pada aspek paleopatologi yang merupakan salah satu kajian ilmu dalam menelusuri jejak kehidupan manusia di masa lalu melalui penyakit pada tulang dan gigi manusia yang ditemukan dalam konteks arkeologi. Materi penelitian dalam artikel ini menitikberatkan pada gigi manusia yang merekam informasi mengenai masa hidup seseorang, termasuk aspek-aspek perkiraan usia saat mati, jenis makanan yang pernah dikonsumsi, dan penyakit yang pernah diderita. Kasus patologi berupa karies menarik untuk diteliti sebab penyakit ini merupakan salah satu kasus yang umum ditemukan pada sisa rangka manusia. Metode penelitian yang digunakan adalah observasi makroskopis dan metode pustaka. Hasil analisis menunjukkan bahwa dari 27 gigi tersisa pada individu ST1, terdapat sembilan gigi yang terdeteksi mengalami karies dan beberapa gigi lain yang menderita penyakit periodontal. Karies pada individu ini tampak disebabkan oleh mikro-organisme yang berkembang di dalam mulut akibat minimalnya perawatan kesehatan gigi dan mulut, serta tidak berhubungan langsung dengan asupan nutrisi yang dikonsumsi oleh individu ini pada masa hidupnya.
STUDI KASUS PATOLOGI GIGI: KARIES PADA RANGKA MANUSIA ST1, SONG TERUS, PACITAN, JAWA TIMUR ( Dental Pathology Case Study: Caries On ST1 Human Remains, Song Terus, Pacitan, East Java) Anita Tamu Ina; Dyah Prastiningtyas; Harry Widianto; Florent Détroit; Ferry Fredy Karwur; Andri Purnomo; Anne-Marie Sémah; François Sémah
Jurnal Penelitian Arkeologi Papua dan Papua Barat Vol. 10 No. 2 (2018): November 2018
Publisher : BALAI ARKEOLOGI PAPUA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2137.762 KB) | DOI: 10.24832/papua.v10i2.253

Abstract

Human remains found in Song Terus (Pacitan, East Java), known as ST1, presented an opportunity of in-depth study in reconstructing how human lived during Early Holocene period in the area. This article focuses on palaeopathological aspects by examining lesions of disease observable in bones and dentition of human remains found in archaeological context. The research done for this article focuses more on dental remains, as teeth are known to have durability and longevity as archaeological finds, and could also provide information on age-at-death, types of diet, and oral diseases which may occurred during a person’s life. Dental caries is one of the most common type of oral disease found in archaeological context. Research methods used are macroscopic observation and literature reference comparison.. Results showed there were nine dentition on this individual (from a total of 27 identified dentition) suffered from caries with various degree of severity. Other types of oral disease noted during observation and analysis were periodontal disease. ST1 might have been suffering from severe caries due to lack of oral hygiene, as well as minimum dental treatment towards emerging oral disease. Nevertheless, these diseases did not seem to be directly caused by ST1’s dietary habit during lifetime. ABSTRAK Temuan rangka manusia ST1 di Song Terus (Pacitan, Jawa Timur) memberikan peluang untuk menelusuri lebih jauh pola kehidupan manusia pada periode Holosen Awal di wilayah ini. Artikel ini berfokus pada aspek paleopatologi yang merupakan salah satu kajian ilmu dalam menelusuri jejak kehidupan manusia di masa lalu melalui penyakit pada tulang dan gigi manusia yang ditemukan dalam konteks arkeologi. Materi penelitian dalam artikel ini menitikberatkan pada gigi manusia yang merekam informasi mengenai masa hidup seseorang, termasuk aspek-aspek perkiraan usia saat mati, jenis makanan yang pernah dikonsumsi, dan penyakit yang pernah diderita. Kasus patologi berupa karies menarik untuk diteliti sebab penyakit ini merupakan salah satu kasus yang umum ditemukan pada sisa rangka manusia. Metode penelitian yang digunakan adalah observasi makroskopis dan metode pustaka. Hasil analisis menunjukkan bahwa dari 27 gigi tersisa pada individu ST1, terdapat sembilan gigi yang terdeteksi mengalami karies dan beberapa gigi lain yang menderita penyakit periodontal. Karies pada individu ini tampak disebabkan oleh mikro-organisme yang berkembang di dalam mulut akibat minimalnya perawatan kesehatan gigi dan mulut, serta tidak berhubungan langsung dengan asupan nutrisi yang dikonsumsi oleh individu ini pada masa hidupnya.
STUDI KASUS PATOLOGI GIGI: KARIES PADA RANGKA MANUSIA ST1, SONG TERUS, PACITAN, JAWA TIMUR ( Dental Pathology Case Study: Caries On ST1 Human Remains, Song Terus, Pacitan, East Java) Anita Tamu Ina; Dyah Prastiningtyas; Harry Widianto; Florent Détroit; Ferry Fredy Karwur; Andri Purnomo; Anne-Marie Sémah; François Sémah
Jurnal Penelitian Arkeologi Papua dan Papua Barat Vol. 10 No. 2 (2018): November 2018
Publisher : BALAI ARKEOLOGI PAPUA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2137.762 KB) | DOI: 10.24832/papua.v10i2.253

Abstract

Human remains found in Song Terus (Pacitan, East Java), known as ST1, presented an opportunity of in-depth study in reconstructing how human lived during Early Holocene period in the area. This article focuses on palaeopathological aspects by examining lesions of disease observable in bones and dentition of human remains found in archaeological context. The research done for this article focuses more on dental remains, as teeth are known to have durability and longevity as archaeological finds, and could also provide information on age-at-death, types of diet, and oral diseases which may occurred during a person’s life. Dental caries is one of the most common type of oral disease found in archaeological context. Research methods used are macroscopic observation and literature reference comparison.. Results showed there were nine dentition on this individual (from a total of 27 identified dentition) suffered from caries with various degree of severity. Other types of oral disease noted during observation and analysis were periodontal disease. ST1 might have been suffering from severe caries due to lack of oral hygiene, as well as minimum dental treatment towards emerging oral disease. Nevertheless, these diseases did not seem to be directly caused by ST1’s dietary habit during lifetime. ABSTRAK Temuan rangka manusia ST1 di Song Terus (Pacitan, Jawa Timur) memberikan peluang untuk menelusuri lebih jauh pola kehidupan manusia pada periode Holosen Awal di wilayah ini. Artikel ini berfokus pada aspek paleopatologi yang merupakan salah satu kajian ilmu dalam menelusuri jejak kehidupan manusia di masa lalu melalui penyakit pada tulang dan gigi manusia yang ditemukan dalam konteks arkeologi. Materi penelitian dalam artikel ini menitikberatkan pada gigi manusia yang merekam informasi mengenai masa hidup seseorang, termasuk aspek-aspek perkiraan usia saat mati, jenis makanan yang pernah dikonsumsi, dan penyakit yang pernah diderita. Kasus patologi berupa karies menarik untuk diteliti sebab penyakit ini merupakan salah satu kasus yang umum ditemukan pada sisa rangka manusia. Metode penelitian yang digunakan adalah observasi makroskopis dan metode pustaka. Hasil analisis menunjukkan bahwa dari 27 gigi tersisa pada individu ST1, terdapat sembilan gigi yang terdeteksi mengalami karies dan beberapa gigi lain yang menderita penyakit periodontal. Karies pada individu ini tampak disebabkan oleh mikro-organisme yang berkembang di dalam mulut akibat minimalnya perawatan kesehatan gigi dan mulut, serta tidak berhubungan langsung dengan asupan nutrisi yang dikonsumsi oleh individu ini pada masa hidupnya.
STUDI KASUS PATOLOGI GIGI: KARIES PADA RANGKA MANUSIA ST1, SONG TERUS, PACITAN, JAWA TIMUR ( Dental Pathology Case Study: Caries On ST1 Human Remains, Song Terus, Pacitan, East Java) Anita Tamu Ina; Dyah Prastiningtyas; Harry Widianto; Florent Détroit; Ferry Fredy Karwur; Andri Purnomo; Anne-Marie Sémah; François Sémah
Jurnal Penelitian Arkeologi Papua dan Papua Barat Vol. 10 No. 2 (2018): November 2018
Publisher : BALAI ARKEOLOGI PAPUA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2137.762 KB) | DOI: 10.24832/papua.v10i2.253

Abstract

Human remains found in Song Terus (Pacitan, East Java), known as ST1, presented an opportunity of in-depth study in reconstructing how human lived during Early Holocene period in the area. This article focuses on palaeopathological aspects by examining lesions of disease observable in bones and dentition of human remains found in archaeological context. The research done for this article focuses more on dental remains, as teeth are known to have durability and longevity as archaeological finds, and could also provide information on age-at-death, types of diet, and oral diseases which may occurred during a person’s life. Dental caries is one of the most common type of oral disease found in archaeological context. Research methods used are macroscopic observation and literature reference comparison.. Results showed there were nine dentition on this individual (from a total of 27 identified dentition) suffered from caries with various degree of severity. Other types of oral disease noted during observation and analysis were periodontal disease. ST1 might have been suffering from severe caries due to lack of oral hygiene, as well as minimum dental treatment towards emerging oral disease. Nevertheless, these diseases did not seem to be directly caused by ST1’s dietary habit during lifetime. ABSTRAK Temuan rangka manusia ST1 di Song Terus (Pacitan, Jawa Timur) memberikan peluang untuk menelusuri lebih jauh pola kehidupan manusia pada periode Holosen Awal di wilayah ini. Artikel ini berfokus pada aspek paleopatologi yang merupakan salah satu kajian ilmu dalam menelusuri jejak kehidupan manusia di masa lalu melalui penyakit pada tulang dan gigi manusia yang ditemukan dalam konteks arkeologi. Materi penelitian dalam artikel ini menitikberatkan pada gigi manusia yang merekam informasi mengenai masa hidup seseorang, termasuk aspek-aspek perkiraan usia saat mati, jenis makanan yang pernah dikonsumsi, dan penyakit yang pernah diderita. Kasus patologi berupa karies menarik untuk diteliti sebab penyakit ini merupakan salah satu kasus yang umum ditemukan pada sisa rangka manusia. Metode penelitian yang digunakan adalah observasi makroskopis dan metode pustaka. Hasil analisis menunjukkan bahwa dari 27 gigi tersisa pada individu ST1, terdapat sembilan gigi yang terdeteksi mengalami karies dan beberapa gigi lain yang menderita penyakit periodontal. Karies pada individu ini tampak disebabkan oleh mikro-organisme yang berkembang di dalam mulut akibat minimalnya perawatan kesehatan gigi dan mulut, serta tidak berhubungan langsung dengan asupan nutrisi yang dikonsumsi oleh individu ini pada masa hidupnya.
STUDI KASUS PATOLOGI GIGI: KARIES PADA RANGKA MANUSIA ST1, SONG TERUS, PACITAN, JAWA TIMUR ( Dental Pathology Case Study: Caries On ST1 Human Remains, Song Terus, Pacitan, East Java) Anita Tamu Ina; Dyah Prastiningtyas; Harry Widianto; Florent Détroit; Ferry Fredy Karwur; Andri Purnomo; Anne-Marie Sémah; François Sémah
Jurnal Penelitian Arkeologi Papua dan Papua Barat Vol. 10 No. 2 (2018): November 2018
Publisher : BALAI ARKEOLOGI PAPUA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2137.762 KB) | DOI: 10.24832/papua.v10i2.253

Abstract

Human remains found in Song Terus (Pacitan, East Java), known as ST1, presented an opportunity of in-depth study in reconstructing how human lived during Early Holocene period in the area. This article focuses on palaeopathological aspects by examining lesions of disease observable in bones and dentition of human remains found in archaeological context. The research done for this article focuses more on dental remains, as teeth are known to have durability and longevity as archaeological finds, and could also provide information on age-at-death, types of diet, and oral diseases which may occurred during a person’s life. Dental caries is one of the most common type of oral disease found in archaeological context. Research methods used are macroscopic observation and literature reference comparison.. Results showed there were nine dentition on this individual (from a total of 27 identified dentition) suffered from caries with various degree of severity. Other types of oral disease noted during observation and analysis were periodontal disease. ST1 might have been suffering from severe caries due to lack of oral hygiene, as well as minimum dental treatment towards emerging oral disease. Nevertheless, these diseases did not seem to be directly caused by ST1’s dietary habit during lifetime. ABSTRAK Temuan rangka manusia ST1 di Song Terus (Pacitan, Jawa Timur) memberikan peluang untuk menelusuri lebih jauh pola kehidupan manusia pada periode Holosen Awal di wilayah ini. Artikel ini berfokus pada aspek paleopatologi yang merupakan salah satu kajian ilmu dalam menelusuri jejak kehidupan manusia di masa lalu melalui penyakit pada tulang dan gigi manusia yang ditemukan dalam konteks arkeologi. Materi penelitian dalam artikel ini menitikberatkan pada gigi manusia yang merekam informasi mengenai masa hidup seseorang, termasuk aspek-aspek perkiraan usia saat mati, jenis makanan yang pernah dikonsumsi, dan penyakit yang pernah diderita. Kasus patologi berupa karies menarik untuk diteliti sebab penyakit ini merupakan salah satu kasus yang umum ditemukan pada sisa rangka manusia. Metode penelitian yang digunakan adalah observasi makroskopis dan metode pustaka. Hasil analisis menunjukkan bahwa dari 27 gigi tersisa pada individu ST1, terdapat sembilan gigi yang terdeteksi mengalami karies dan beberapa gigi lain yang menderita penyakit periodontal. Karies pada individu ini tampak disebabkan oleh mikro-organisme yang berkembang di dalam mulut akibat minimalnya perawatan kesehatan gigi dan mulut, serta tidak berhubungan langsung dengan asupan nutrisi yang dikonsumsi oleh individu ini pada masa hidupnya.
ZEASANTIN DARI MIKROALGA: BIOSINTESIS DAN PEMANFAATANNYA Dispanstiani Abidin; Ferry Karwur
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 3 (2009): December 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i3.154

Abstract

Karotenoid merupakan pigmen alami yang disintesis oleh semua organisme fotosintetik, serta sejumlah bakteri dan jamur non-fotosintetik. Ada dua pengelompokan karotenoid alami: (1) karoten, seperti â-karoten dan á-karoten, dan (2) santofil. Umumnya santofil seperti violasantin, antherasantin, zeasantin, neosantin, dan lutein diproduksi oleh tumbuhan tingkat tinggi, namun juga dapat disintesis oleh mikroalga hijau.  Zeasantin merupakan salah satu pigmen yang berperan penting  dalam  pencegahan  penyakit  degenerasi  makular  akibat  usia (AMD). Produksi zeasantin dapat  dilakukan oleh  beberapa mikroalga seperti Spirulina sp., Dunaliella salina, Chlamydomonas reinhardtii, Synechocystis, Microcystis aeruginosa, dan Porphyridium cruentum. Artikel ini akan membahas mengenai biosintesis zeasantin serta pemanfaatannya.
Astaxanthin produced by marine bacteria: biosynthesis, uses, and the potency of mass production Naely Kurnia Wusqy; Ferry Fredy Karwur
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 1 (2010): May 2010
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.125 KB) | DOI: 10.15578/squalen.v5i1.44

Abstract

Astaxanthin (3,3‘-dihydroxy-β, β-carotene-4, 4’-dione) is an orange-red xanthophylls that contains 40 carbon atoms which is connected by single and double bonds to form fitoen chains inwhich their all-trans isomer are found in nature together with a small amount of 9-cis and 13-cisisomers. Fitoen chains of astaxanthin begin and end by ionon chains. Astaxanthin belongs to thexanthophylls group because it has oxygen rings. Some marine bacteria are reported to produceastaxanthin i.e Brevundimonas, Paracoccus hundaenensis, Alcaligenes andAgrobacteriumaurantiacum. This paper describes astaxanthin production in marine bacterial cells including itsbiosynthesis from β-carotene conversion and enzyme taking a role in this biosynthesis, and itsmass production for commercial purposes. This review also describes about their uses for foodand health purposes.
Design of biology teaching materials based on discovery learning with scientific approach for class 10th in senior high school Binerd Anthon Im Toy; Ferry F Karwur; Junet F. da Costa; Jerry F Langkun; Ferdy S Rondonuwu
Biosfer: Jurnal Pendidikan Biologi Vol 11 No 1 (2018): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.211 KB) | DOI: 10.21009/biosferjpb.11-1.7

Abstract

Indonesia as a tropical country with two seasons and has a high humidity into a good land for mushrooms growth. Students need to be taught to understand mushrooms. Understanding includes; morphology, physiology, reproduction, roles, and benefits of fungi. Discovery learning with a scientific approach becomes one of the active methods for, providing learning to Senior high school-class 10th students. Methods of scientific approach are done through activities: observing, asking, trying, reasoning, and communicating. A biology lesson that is active, innovative, creative, effective, and fun for the learners depends on the approach chosen and the instructional method used. The purpose of the development of this learning material was to design a biology instructional module on fungi using discovery learning with scientific approach. A scientific approach is a learning approach conducted through the following activities: observing, asking questions, experimenting, analyzing, and communicating. One method that is relevant with the principles of scientific approach is discovery learning. The stages in discovery learning are: stimulation, problem identification, data collection, data processing, providing evidence, and drawing conclusion. This biology lesson based on discovery learning with scientific approach complemented with pictures has the potentials in increasing the learning of the learners because they will be able to organize and conduct experiments to discover new knowledge
ISOLASI DAN IDENTIFIKASI KOMPONEN KIMIA MINYAK ASIRI DAGING BUAH DAN FULI BERDASARKAN UMUR BUAH PALA (MYRISTICA FRAGRANS HOUTT) Angreni B. Liunokas; Ferry F. Karwur
Jurnal Biologi Tropis Vol. 20 No. 1 (2020): Januari - April
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (571.907 KB) | DOI: 10.29303/jbt.v20i1.1651

Abstract

Abstrak: Pala (Myristica fragrans Houtt) sebagai bioreaktor hayati menghasilkan produk utama minyak asiri bernilai ekonomi tinggi yang tersebar pada bagian-bagian buahnya. Minyak asiri pala tersusun oleh kelompok besar terpenoid dan fenilpropanoid yang memiliki konsentrasi terkecil namun sebagai indikator mutu minyak pala. Penelitian tentang isolasi dan identifikasi komponen kimia minyak asiri daging buah dan fuli pala, telah dilaksanakan di Laboratorium CARC UKSW Salatiga dan Laboratorium Terpadu UII Yogyakarta. Penelitian ini bertujuan untuk mengetahui komponen kimia minyak asiri daging buah dan fuli pala berdasarkan tahapan perkembangan buah dan isolasi miristisin sebagai indikator dari kualitas minyak pala. Minyak asiri diperoleh dengan metode ekstraksi kemudian di analisis komponennya menggunakan Kromatografi Gas Spektrometri Massa (GC-MS). Hasil penelitian menunjukkan bahwa komponen kimia terbesar dari minyak asiri daging buah pala secara berturut-turut yaitu tahap pertama miristisin 36.05% pada retensi 11.57 menit, tahap kedua dan keempat α-pinen 39.16%; 34.64% retensi 3.53 menit, tahap ketiga sabinen 33.88% pada retensi 3.99 menit. Sedangkan pada minyak fuli tahap pertama hingga keempat yaitu sabinen 38.72%; 37.51%; 36.24%; 28.93% pada waktu retensi 3.99. Berdasarkan analisis GC-MS menunjukkan bahwa komponen senyawa yang dominan pada daging buah dan fuli yaitu miristisin, sabinen, α-pinen, β-pinen yang berkontribusi bagi aroma khas pada pala. Hasil penelitian diharapkan dapat dimanfaatkan sebagai sumber informasi penting dalam menghasilkan minyak asiri dengan kualitas baik dengan kandungan miristisin terbanyak.Kata Kunci : M. fragrans Houtt; isolasi; daging buah, fuli, minyak asiriAbstract: Nutmeg (Myristica fragrans Houtt) as a natural bioreactor produces the main product that is aetheric oil with a high economic value which is scattered on the parts of the fruit. Aetheric oil consists of a big group of terpenoid and phenylpropanoid which has the smallest concentration but functions as the indicator of nutmeg oil quality. The research about isolation and identification of chemical components of aetheric oil of pulp and mace has been conducted in CARC Laboratory of UKSW Salatiga and Integrated Laboratory of UII Yogyakarta. This current research aims at knowing the components of aetheric oil of nutmeg pulp and mace based on the development stages of the fruit and myristicin isolation as the indicator of the quality of nutmeg oil. Aetheric oil is formed by the extraction method. Then the components are analyzed by using Mass Spectrometry Gass Chromatography (GC-MS). The result of the research shows that respectively the biggest chemical component of aetheric oil consists of the first stage myristicin 36.05% on retention 11.57 minutes, second stage and fourth stage α-pinene 39.16%; 34.64% on retention 3.53 minutes, the third stage sabinene 33.88% on retention 3.99 minutes. Meanwhile on the mace oil, the first to the fourth stage is sabinene 38.72%; 37.51%; 36.24%; 28.93% on retention 3.99. Based on the analysis of GC-MS, it is shown that the most dominant compound components of pulp and mace are myristicin, sabinene, α-pinene, and β-pinene which contribute to the typical scent of nutmeg. The result of the research is expected to be used as an important information source in resulting in aetheric oil with good quality with the most myristicin content.Keywords: Myristica fragrans Houtt; isolation; pulp, mace, aetheric oil
PENELUSURAN SENYAWA BIOAKTIF BAKTERI YANG BERASOSIASI DENGAN ANEMON LAUT SERTA IDENTIFIKASI MOLEKULERNYA BERBASIS 16S rDNA Naely K Wusqy; Dwi I Prayitmo; Ocky K Radjasa; Leenawaty Limantara; Ferry F Karwur1
Jurnal Perikanan Universitas Gadjah Mada Vol 14, No 1 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9049

Abstract

Sea anemone-associated microorganisms are among of the most interesting and promising marine natural product sources, which produce various biological activities. In this study, marine bacteria were isolated from 2 kinds of sea anemones, Entacmaea medusivora and Green Anemone that were collected from Bandengan, Jepara and Maratua, East Borneo respectively. Nine bacterial isolatesof E.medusivora were screened for antibacterial activity against 8 strains. One out of 9 bacterial isolates was successfully screened and was found to be active against V. parahaemolyticus strains. This active isolate was also capable of amplifying PKS gene fragments necessary for the biosynthesis of polyketide synthase. The identifi cation results revealed that the active isolate is Bacillus cereus. One bacterial isolate of Green Anemone was screened to produce the pigment and positively synthesize carotenoids. Initial analysis with atomic absorption spectrophotometric method revealed that the wave length of bacterial pigment were in the range of 300-600 nm, which are categorized that within the group of carotenoid pigments. From the results of molecular identifi cation by 16S rDNA method, it was shown that bacterium was closely related to Xanthomonas sp.