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All Journal Jurnal Teknologi Dan Industri Pangan Microbiology Indonesia Alchemy : Journal of Chemistry Agrointek Journal of Food and Life Sciences Jurnal Pangan dan Agroindustri Jurnal Teknologi Pertanian Natural B Indonesian Journal of Human Nutrition agriTECH Majalah Kedokteran Sriwijaya BIOMA : Jurnal Biologi Makassar BIOEDUSCIENCE TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian Indonesian Journal of Halal Research Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) ZOOTEC Jurnal Ilmiah Pengabdian Kepada Masyarakat Journal of Research on Community Engagement
Joni Kusnadi
Fakultas Teknologi Pertanian, Universitas Brawijaya, Malang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : BIOEDUSCIENCE

 1 
HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.134 KB) | DOI: 10.22236/j.bes/425193

Abstract

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.
HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/425193

Abstract

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.
HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/425193

Abstract

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.
Co-Authors A'rasyiJ Imaduddin A.A. Ketut Agung Cahyawan W Adistia Ritri Prihandini Adistia Ritri Prihandini, Adistia Ritri Afifah Puji Hastuti Afifah Puji Hastuti, Afifah Puji Afla Tazakka Aj-juwita Afla Tazakka Aj-juwita Afriliana, Asmak AGUSTIN KRISNA WARDANI Agustin Mardiana, Nur Ahmad Zakiyul Wafi Ainina Ahmad Santoso Ali Masykur Ali Masykur Andayani, Dian Wuri Anik Ardianti Anik Ardianti Atmiral Ernes Ayu Fitriana Wulandari Baiq Dina Mariana Benediktus Hendra Perkasa Citra Firdhausi Citra Firdhausi Daimon Syukri Dedi Dedi Denta, Anggeria Oktavisa Dian Widya Ningtyas Dila Rahmawati Dr.Ir. Yunianta, DEA Dwi Esti Prayanti Dwi Ratnaningsih DWI SURYANTO Edi Priyo Utomo Elke Galuh Primurdia Elke Galuh Primurdia Elok Zubaedah Elok Zubaidah Endrika Widyastuti Endrika Widyastuti Eni Purwanti Eni Purwanti, Eni Erni Sofia Muritni Erni Sofia Murtini Erryana Martati Estri Laras Arumingtyas Estri Laras Arumingtyas Eunike Debora Purba Fadri, Rince Alfia Faiqotur Rohmania Feronika Heppy Sriherfyna Fitri Nurzanah Fitriyana, Nurul Isnaini Freini Dessi Efendi Hakiki, Hilda Maghfirotu Hamidin Rasulu Hana Susanti Maleta Hannes Dwi Novia Hariry, Amelia Hasria Alang Herlambang, Dwi Hernandi, Kevin Hohn Hilda Maghfirotu Hakiki Ima Andriastuti IS HELIANTI Ito, Nobutaka Jamhari Jamhari Jaya Mahar Maligan Kartika Cahyania Pratiwi Kevin Hohn Hernandi Khairul Ambri Khotibul Umam Al-Awwaly Khotibul Umam Al-Awwaly Khusnul Khotimah Khusnul Khotimah KIKI FIBRIANTO Lenny Yudha Febriyanti Lenny Yudha Febriyanti Lia Ratnawati Lilis Karlina Malsa Fian Yana Malsa Fian Yana Mega Kristanti Ayuratri Muhammad Reza Ramadhana Muhammad Reza Ramadhana, Muhammad Reza Nazir, N Nenu Maria Ovy Sonia Nenu Maria Ovy Sonia Nisa Harmia Yuliasih Noor Hidayat, Noor Novi Vensia Magdalena Novi Vensia Magdalena Novita Wijayanti Nur Istianah Nur Istianah Nur Kusmiyati Pamulanti, Anjar Pebiningrum, Arlinda Pendik Setiawan Pratiwi Anggun Retnowati Pratiwi Anggun Retnowati Putri, Annisyia Zarina Raida Amelia Ifadah Raida Amelia Ifadah Rif'ah, Hamidah Itha'atur RIMA AZARA Rinelda Ayu Sintasari Rinelda Ayu Sintasari Rukmi, Widya Dwi Septi, Nur Diana Shanty Karinawatie Sudarma Dita Wijayanti Sudarma Dita Wijayanti Sudarminto Setyo Yuwono Suharjono Suharjono Suharjono Suharjono, Suharjono Sutik Rahayu Endah Sutrisno Adi Prayitno Suwasono, Sony Tabitha Larasati Tabitha Larasati Tanti Untari Tanti Untari Teti Estiasih Titis Sari Kusuma Tri Ardyati Tri Ardyati Try Ardyati Tutik Murniasih Tuty Anggraini Vivi Retno Sari Vivi Retno Sari Widya Dwi Rukmi Putri Yan Abdi Nugroho Yan Abdi Nugroho Yohana Lydia Rissa Tanjung Yohana Lydia Rissa Tanjung Zuliyaningsih Zuliyaningsih