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All Journal Jurnal Teknologi Dan Industri Pangan Natural Science: Journal of Science and Technology JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Reaktor Jurnal Natur Indonesia Microbiology Indonesia AL KAUNIYAH Jurnal Matematika & Sains Jurnal Pangan dan Agroindustri agriTECH Indonesian Journal of Cancer Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Indonesian Journal of Chemistry Akuatik: Jurnal Sumberdaya Perairan Jurnal Perikanan Universitas Gadjah Mada Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Natural Pigments Cermin Dunia Kedokteran Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Indonesian Journal of Cancer Jurnal Buana Pengabdian JURNAL REKAYASA KIMIA & LINGKUNGAN

Leenawaty Limantara

Universitas Pembangunan Jaya

Author-ID : 305498

Religion Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Other

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Analisis Komposisi dan Kandungan Karotenoid Total dan Vitamin A Fraksi Cair dan Padat Minyak Sawit Kasar (CPO) Menggunakan KCKT Detektor PDA Syahputra, M. Rio; Karwur, Ferry F; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

This study was carried out on two phases of Crude Palm Oil (CPO) to determine the total and composition ofcarotenoid and vitamin A content. Total of carotenoid was analyzed using spectrophotometer UV-Vis, and then theresult was calculated by Gross (1991) equation. The vitamin A content was calculated by NAS-NRC equation (1974).The type and composition of both phases of CPO were determined by Choo’s method (1994) by using HPLC withPhoto Diode Array (PDA) detector. The sample was prepared in two methods, with and without saponification. Theresult shows that total carotenoids in liquid and solid phase of CPO are 536 ± 13.2 g/g (liquid), 352 ± 17.7 μg/g(solid) and the vitamin A were 89.4 ± 2.2 RE (liquid), 58.7 ± 3.0 RE (solid), respectively. The carotenoid compositionsof both phases of CPO were dominated by - and -carotenes. The result shows that - and -carotenes preparedby saponification method in liquid phase are 29.03% and 60.88%, and without saponification (direct method) are28.14% and 59.44%. The result for solid phase shows that - and -carotenes by saponification are 25.89% and60.81%, and without saponification (direct method) are 30.00% and 56.92%. The research also shows the advantagesof using HPLC with PDA detector for identification and analysis of type and carotenoid composition.

Fotoproteksi Kurkumin terhadap β-Karoten pada Berbagai Nisbah Molar serta Aktivitas Antioksidannya Natalina, Elly; Rahayu, Puji; Sulistyowati, Sulistyowati; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Curcumin and β-carotene are two kinds of pigments serve as antitumor, anticancer and antioxidant agents. Theantioxidant activity of curcumin is stronger and more stable as compared to β-carotene, so it can protect β-carotene degradation from light and oxygen. Therefore curcumin has higher antioxidant activity as well. Theresearch was conducted by mixing curcumin and β-carotene in several molarity ratios followed by iradiation usingvolpi lamp for 0, 10, 30, 60, and 90 minutes. The result shows that curcumin has photoprotection effect because itcan protect photooxidation of β-carotene. Mixing of curcumin and β-carotene 1:1 was the most stable ratio forprotecting β-carotene.

Efek Beta Karoten dan Agregasi Klorofil Pada Fotostabilitas Klorofil a dalam Pelarut Aseton Costa, Junet. F. da; Karwur, Ferry F; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Chlorophyll as photosynthetic pigment has many benefits to human such as antioxidant, antibacterial, color additive,immunity, and photosensitizer. Concerning to these applications, chlorophyll a will react with oxygen and light andreduce its effectiveness. It is then important to understand the stability of chlorophyll a on oxygen and light.Stability of chlorophyll a in the presence of beta-carotene (1:1) in acetone was studied for various waterconcentrations. Each solution was exposed to red light (ë e” 630 nm) for 0.5; 1.0; 2.5; 5.0; 10; 20; 30; 40; 50; and 60minutes and their spectrum were analysed. When water was added (8.33; 16.67; 25; 33.33; 41.67; 50; 58.33; 66.67;75; and 83.33 percent) to the acetone solution of chlorophyll a, the chlorophylls aggregate, and in the presence ofbeta-carotene, the chlorophyll more stable. The water shifts the chlorophyll a spectrum toward red duringillumination with or without the presence of carotene. The formation of oligomeric chlorophyll a aggregate occurswithin 10-20 minutes after the water was applied. This strategy of aggregating the chlorophylls to dissipateexcess energy captured from light does not support the photostability of chlorophyll as much as beta-carotene.Surprisingly, when beta carotene was mixed with 66.7% and 75.0% of water, the chlorophyll a degraded 5.56% and9.71% respectively. In conclusion, the aggregate form of chlorophyll a and the presence of beta-carotene increasephotostability of chlorophyll a in acetone solution.

Thermal Degradation Kinetics of Encapsulated Palm Carotenes Using Different Combination of Wall Materials Hong, Khoo Mun; Indrawati, Renny; Brotosudarmo, Tatas HP; Limantara, Leenawaty
Indonesian Journal of Natural Pigments Vol 2 No 1 (2020): February 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.1.21

Palm oil is known as one of the richest sources of carotenes. Carotenes concentrate could be prepared from crude palm oil and possessing potency to be used as nutraceutical materials. Due to the susceptibility of carotenes against heat, the encapsulation procedure might be applied to provide protections. The present study was aimed to learn the influence of using different combination of wall materials toward the thermal degradation kinetics of palm carotenes. Maltodextrin was used as primary wall material, being combined with various surface-active agents, i.e. Tween-80 (A), Tween-20 (B), and Arabic gum (C). The carotenes concentrate was encapsulated through emulsification steps, and the vivid yellow powder was obtained after freeze drying process. Then, encapsulated palm carotenes were stored in 200C, 350C, and 500C for several days, and the color, carotenes spectrum, as well as water content were monitored periodically. The kinetics of thermal degradation of carotenes were estimated using Arrhenius equation. The results showed that the use of different combination of wall materials exhibited distinct degradation rate. The combination of maltodextrin and Tween-80 gave preferable protection compared to the others, being half-degraded after 51 days in 200C.

Carotenoid Analysis of Ripe Banana Flesh and Peel from Three Cultivars of Banana Septiany, Gabriela Johana; Putri, Widya Dwi Rukmi; Panca, Ida Nur; Heriyanto, Heriyanto; Limantara, Leenawaty
Indonesian Journal of Natural Pigments Vol 1 No 2 (2019): Agustus 2019
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2019.01.2.60

Banana peel is a promising source to be utilized in fortification of food products due to the high content of carotenoids (Cars) which are active as provitamin A. Dominant, total and relative concentrations of Cars were determined from fresh flesh and peel in three cultivars of banana obtained from Raja, Ambon Kuning, and Kepok Kuning. To evaluate the difference in Car compositions principal component analysis (PCA) was also performed. Based on chromatographic, spectroscopic, and mass spectrometric analyses, dominant Cars were separated and identified to be lutein, Î±-carotene and Î²-carotene. Lutein was the major Car of fresh peel, while other two Cars were found in fresh flesh in addition to lutein. Raja banana had the highest total concentration of Cars among three banana samples used. PCA results generated from the absorption spectra showed three clusters of the different banana samples. PCA results are correlated to their Car compositions and this method might be applicable for the determination of dominant Cars.

Humansâ€™ Body Immunity and Natural Pigments Indrawati, Renny; Limantara, Leenawaty; Brotosudarmo, Tatas H.P.
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.26

The emerging pandemic of Corona Virus Disease (COVID-19) has attracted special concerns regarding the case of very contagious viral infection. Besides the profound waiting toward vaccine development, public awareness was also addressed into the effort of increasing humansâ€™ body immunity. Nowadays, the people are encouraged to consume adequate amount of vitamin C and increase the consumption of fruits and vegetables. In addition, various kinds of food supplements are offered and claimed to give us protection and prevention against viral infection by boosting our immune system. However, the communities are actually lack of proper information regarding the true mechanism of immune system. How is the infection developed in our body? How does the immune system work? Could it be modulated by consuming the bioactive compounds? Here, the stages of infection, the immune system, as well as the role of natural pigments to support the humansâ€™ body immunity will be briefly discussed.

Color Alteration of Encapsulated Beetroot (Beta vulgaris L.) Extract Upon Dissolving in Various pH Treatment Lukitasari, Diah Mustika; Indrawati, Renny; Chandra, Rosita Dwi; Heriyanto, Heriyanto; Limantara, Leenawaty
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.48

It has been known that most natural pigments are unstable upon exposure against several environmental factors, such as thermal treatment, light, oxidizing or reducing agents, as well as acid or alkaline compounds. Encapsulation procedures is often adopted to adjust the solubility and provide protection to the natural pigments. Here, we prepared an encapsulated beetroot extract as the candidate of red bio-colorant. The primary aim of the present study is to investigate color alteration of encapsulated beetroot (Beta vulgaris L.) extract upon dissolving in various pH adjustment and prolonged storage. The McIllvainne buffer was prepared in various pH range, i.e. from 2 to 11. The encapsulated extract (0.1% w/v) was dissolved and the color of the solutions were measured regularly until 3 days storage under darkness at 200C. Any degradation or structural changes will cause color alteration, which were monitored through L*, a*, b* values, the hue angle (HÂ°), chroma values (C), as well as color difference (DE). The results showed that pH 4 was the most favorable condition that brings least impact to the color alteration, even when the colored solution was kept in prolonged storage until 9 days.

Terapi Alternatif Penyakit Kardiovaskuler dengan Pigmen Alami Salim, Katarina Purnomo; Indrawati, Renny; Limantara, Leenawaty
Cermin Dunia Kedokteran Vol 43, No 11 (2016): Kesehatan Ibu - Anak
Publisher : PT. Kalbe Farma Tbk.

Pola diet dan gaya hidup kurang sehat menyebabkan berbagai kasus hiperkolesterol dan obesitas. Berbagai pengobatan untuk mengatasi permasalahan tersebut masih terus diperbaharui, tak hanya dengan obat-obatan sintetik namun juga obat alam berbasis senyawa bioaktif tumbuhan tingkat tinggi. Sejumlah studi juga menunjukkan bahwa pigmen alami, yang diproduksi oleh tumbuhan tingkat tinggi maupun rendah, juga mampu memberikan manfaat terapi hampir serupa untuk membantu menurunkan prevalensi penyakit kardiovaskular. Beberapa pigmen seperti fukosantin, astasantin, lutein, dan fikosianin dibuktikan memiliki sifat antioksidan alami serta bioaktivitas spesifik dalam metabolisme lemak tubuh, sehingga dapat digunakan untuk terapi penyakit yang disebabkan oleh kerusakan oksidatif, termasuk penyakit kardiovaskular.The unhealthy life style and poor diet become the reason of most hypercholesterol and obesity cases. Numerous studies are still continuously conducted to overcome heart conditions, not only with synthetic drugs but also natural herbals from bioactive compounds of higher plants. Several natural pigments studies showed comparable activities in decreasing cardiovascular disease prevalence. Fucoxanthin, astaxanthin, lutein, and phycocyanin are natural antioxidants, playing beneficial role in the metabolism of body fats. They can be applied for diseases associated with oxidative stress, including cardiovascular problems.

MIKROENKAPSULASI PIGMEN DARI KUBIS MERAH: STUDI INTENSITAS WARNA DAN AKTIVITAS ANTIOKSIDAN Diah Mustika Lukitasari; Renny Indrawati; Rosita Dwi Chandra; _ Heriyanto; Leenawaty Limantara
Jurnal Teknologi dan Industri Pangan Vol. 28 No. 1 (2017): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Utilization of artificial food colorants has obtained particular concern for long time consumption. Red cabbage (Brassica oleracea L. var. capitata forma rubra L.) was extracted to produce a candidate for natural food colorant through a one-step physical extraction and microencapsulation. The color strength was determined by measuring: (1) tinctorial strength, (2) color degradation kinetics at various pH, and (3) thermostability. Yield level and antioxidant activity were enclosed as supporting data. The results showed that extract of red cabbage exhibited vivid red until green color at various pH and was nearly stable at pH 2 and 3, meanwhile its thermostability was fairly good at pH 3, 4, 8, and 9. The encapsulated red cabbage extract produced high color intensity at pH 2 for red color and pH 9 for blue color with a yield level of 5.53%. It also showed antioxidant activity with IC50 value of 725.65±0.086 ppm in methanol solvent and 258.25±0.097 ppm in water-methanol solvent.

Pengaruh Metode Nanoenkapsulasi terhadap Stabilitas Pigmen Karotenoid dan Umur Simpan Minyak dari Buah Merah (Pandanus conoideus L) Ferdiansyah Ferdiansyah; Heriyanto Heriyanto; Christofora Hanny Wijaya; Leenawaty Limantara
agriTECH Vol 37, No 4 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The utilization of carotenoid rich red fruit oil for food product development is still limited due to has low solubility in aqueous phase and unstable. Nanoencapsulation was proposed to improve the solubility as well as to retain the stability. This study aimed to determine the capability of nanoencapsulation process in maintaining the stability of carotenoid towards heat and light treatments and to estimate the shelf life of the encapsulant. Ionic gelation method was applied in the nanoencapsulation process using chitosan as a coating agent. Total carotenoid was calculated using Gross equation, while the estimation of shelf life of the product was predicted using Arrhenius model. Particle size of the best nanoencapsulation sample was 70.48 nm with a polydisperse index of 0.02 and zeta potential of 14.80 mV. These physical properties of nanoencapsulant agreed with the results of morphological measurement by TEM. The stability of carotenoid pigment in nanoencapsulant throughout several treatments, such as light (1700 lux, 12 hours), blanching (80 °C, 30 minutes), pasteurization (90 °C, 20 minutes), and sterilization (121 °C, 5 minutes) was higher compared to the stability of emulsion without treatment. The decreasing of total carotenoid in nanoencapsulant for each treatment was 6.92%; 13.51%; 17.77%; and 20.49%, while the reduction in emulsion was 26.33%; 45.25%; 54.46%; and 65.74%, respectively. Nanoencapsulation sample at room temperature (25 °C) has a shelf life of 5.2 months which was longer than the emulsion sample (0.5 months). Nanoencapsulation showed better protection on the stability of carotenoid pigment and increased the shelf life of red fruit oil. ABSTRAKPemanfaatan minyak buah merah yang kaya akan pigmen karotenoid pada produk pangan masih terbatas karena sifatnya kurang larut dalam fase air dan memiliki stabilitas yang rendah. Nanoenkapsulasi diharapkan dapat memperbaiki kelarutan dalam air dan mempertahankan stabilitasnya. Penelitian ini bertujuan untuk mempelajari pengaruh proses nanoenkapsulasi dalam mempertahankan stabilitas pigmen karotenoid minyak buah merah dari pengaruh perlakuan panas dan cahaya, serta memperkirakan umur simpan nanoenkapsulat. Proses nanoenkapsulasi menggunakan metode gelasi dengan kitosan sebagai bahan enkapsulat. Total karotenoid diukur menggunakan persamaan Gross, dan prediksi umur simpan menggunakan model Arrhenius. Ukuran partikel nanoenkapsulasi terbaik mencapai 70,48 nm dengan polidispersi indeks 0,02 dan zeta potensial 14,80 mV. Kriteria fisik nanoenkapsulasi ini didukung oleh hasil pengukuran morfologi menggunakan TEM. Stabilitas pigmen karotenoid pada nanoenkapsulasi dengan perlakuan cahaya (1700 lux, 12 jam), blansir (80 °C, 30 menit), pasteurisasi (90 °C, 20 menit), dan sterilisasi (121 °C, 5 menit) lebih baik dibandingkan emulsi tanpa perlakuan dengan penurunan total karotenoid masing-masing perlakuan sebesar 6,92%; 13,51%; 17,77%; dan 20,49% pada nanoenkapsulasi, sedangkan pada emulsi mencapai 26,33%; 45,25%; 54,46%; dan 65,74%. Sampel nanoenkapsulasi pada suhu ruang (25 °C) memiliki umur simpan 5,2 bulan lebih lama dibandingkan sampel emulsi yang hanya mencapai 0,5 bulan. Metode nanoenkapsulasi dapat mempertahankan stabilitas pigmen karotenoid dan meningkatkan umur simpan minyak buah merah.

Co-Authors A. B. Susanto A.B. SUSANTO AB Susanto Adhiwibawa, Marcelinus A.S. Agus Sabdono Aji Wahyu Budiyanto Amelia Myristi Lolita Antonius Budi Susanto Ayu Nur Aida Bambang Cahyono Budi Prasetyo C Hanny Wijaya Christina Astutiningsih Dece Elisabeth Sahertian Diah Mustika Lukitasari Diah Mustika Lukitasari, Diah Mustika Dian Resita Dian Ridwan Nurdiana Dwi I Prayitmo DWI SUSILANINGSIH EDWIN MAHENDRA Elly Natalina Endrika Widyastuti Ferdiansyah Ferdiansyah Ferry Fredy Karwur Fitriyah Nurhidayah Gabriela Johana Septiany, Gabriela Johana Hana Susanti Maleta Hari Kristopo Helly de Fretes Heriyanto . Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heryanto Heryanto Hong, Khoo Mun Indriatmoko Indriatmoko Jaya Mahar Maligan Junet. F. da Costa Katarina Purnomo Salim Lia Kusmita M. Rio Syahputra Madalena Madalena Maleta, Hana Susanti Maria Ika Putri Soegiarto, Maria Ika Putri Muhammad Diah Khoirul Huda Naely K Wusqy NAELY K. WUSQY Naomi Megananda Mulyadi Naomi Megananda Mulyadi, Naomi Megananda Naurissa Biasini Naurissa Biasini Neltji Herlina Ati Novita Wijayanti Nur Ida Panca Nugrahini Ocky K Radjasa Ocky K. Radjasa Ocky K. Radjasa Ocky Karna Radjasa Prasetyandaru Pirenantyo Prasetyandaru Pirenantyo, Prasetyandaru Pratika Riris Putrianti Pratika Riris Putrianti Puji Rahayu Puji Rahayu Rebecca Christiana Reni Dyanasari Reni Dyanasari Reni Subawati Kusumaningtyas Renny Indrawati Renny Indrawati Renny Indrawati, Renny Reny Pratiwi Rosita Dwi Chandra Rosita Dwi Chandra, Rosita Dwi Salim, Katarina Purnomo Sitepu, Rehmadanta Soenarto Notosoedarmo Soenarto Notosudarmo Sulistyowati Sulistyowati Suryasatriya Trihandaru Susanti Pudji Hastuti Tatas H.P. Brotosudarmo Teti Estiasih Tri Dewanti Widyaningsih Venny Santosa Widya Dwi Rukmi Putri Windu Merdekawati Yundari, Yundari _ Heriyanto

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