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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Teknologi Dan Industri Pangan Sainteks Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biotropika Indonesian Journal of Biotechnology Jurnal Sumberdaya Hayati Indonesian Journal of Chemistry ANNALES BOGORIENSES Marine Research in Indonesia Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Jurnal Tanah dan Iklim Makara Journal of Science Annales Bogorienses
YULIN LESTARI
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Veterinary

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Morphological Characterization and Antioxidant Activity of Actinobacteria from Xylocarpus granatum Growing in Mangrove Habitat Ratte, Marina; Batubara, Irmanida; Lestari, Yulin
Biotropika: Journal of Tropical Biology Vol. 10 No. 1 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.01.01

Abstract

Xylocarpus granatum produces various bioactive compounds with diverse biological activities, one of them as an antioxidant. Endophytic actinobacteria can also produce bioactive compounds. This study aimed to explore the existence of the endophytic actinobacteria of X. Granatum and determine its antioxidant activity. The research method begins with the isolation of endophytic actinobacteria from fruits, seeds, and leaves using selective media Humic Acid (HV). Furthermore, characterizing the obtained isolates using four different media, namely YSA, ISP2, ISP3, and ISP4, measured antioxidant activity for actinobacterial supernatants and actinobacterial ethyl acetate extracts using the DPPH method. The results showed that from X. granatum, 15 isolates of endophytic actinobacteria were obtained from fruit, seeds, and leaf. Endophytic actinobacteria isolates from X. granatum showed different colors of aerial mycelium, substrate mycelium, and spore chain type. XGF11, XGF12, and XGF4 isolates produced high antioxidant activity of the supernatant; 57.30%, 49.77%, and 41.90% inhibition, respectively. The antioxidant capacity of XGF12 ethyl acetate extract was 501.60 μg AEAC/mg extract and 465.47 μg AEAC/mg extract for XGF11 ethyl acetate extract. DNA extraction and 16S rRNA amplification from endophytic actinobacteria X. granatum according to Presto Mini gDNA Bacteria Kit Protocol. Based on 16S rDNA gene analysis, isolates XGF12 and XGF11 showed 99.16% and 99.42% similarities with Streptomyces xylanilyticus, respectively. Meanwhile, XGF4 showed to have 99.82% similarities with Brevibacterium sediminis.
Responses of shallot to ameliorant and actinobacteria applications in water-saturated system on tidal land Haitami, A.; Ghulamahdi, Munif; Sopandie, Didy; Susila, Anas Dinurrohman; Lestari, Yulin
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 1 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i1.50768

Abstract

Tidal areas are believed as future land for many commodities in Indonesia. Here, shallot growing was evaluated on tidal land supplemented with soil conditioners. The research aimed to evaluate the adaptive response of shallot varieties for growing on tidal land with a water-saturated system indicating Fe and Al stress by applying soil ameliorant and actinobacterial. The study used a completely randomized factorial design with three replications. The first factor was shallot variety (Bima Brebes, Batu Ijo, and SS Sakato) and the second factor was soil conditioners (no ameliorant, actinobacteria, ameliorant+actinobacteria, and actinobacteria+ameliorant+dolomite). Bima Brebes and Batu Ijo cultivars had the highest growth percentage, age at shoot emergence, plant height, and number of leaves, while the SS Sakato variety had the slowest growth. Shallot production variable had a significant interaction with soil conditioners. Bima Brebes variety demonstrated high suitability for a water-saturated system in tidal land, and actinobacteria+ameliorant+dolomite was a more promising conditioner than other treatments. Keywords: pyrite, saturated soil culture, tidal swamps, growth, yield, marginal land.
Bacterial Community in Apis cerana and Heterotrigona itama Honey Using a Metabarcoding Approach Nurjanah, Nurjanah; Raffiudin, Rika; Lestari, Yulin
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.310-320

Abstract

Honey is a healthy, natural product with high nutritional value that is converted from sugar in nectar by bacteria in the honey stomach of the bees. Several beneficial bacteria in honey produce bioactive compounds, such as Lactobacillus in Apis mellifera honey, which synthesizes lactic acid, bacteriocins, and enzymes. Here, we employed the metabarcoding technique using the 16S rRNA gene to identify the bacterial community in honey from A. cerana and Heterotrigona itama collected in Sukabumi Regency, West Java, Indonesia. Genomic DNA from both honey samples was isolated using the ZymoBIOMICSTM DNA miniprep kit before sequencing with the Oxford Nanopore Technologies (ONT) platform. Our studies showed that the most dominant bacteria in the honey of A. cerana and H. itama were Paenibacillus glucanolyticus and Limosilactobacillus, respectively. In both types of honey, Gram-positive and Gram-negative bacteria were also detected, as well as lactic acid bacteria, including Acetilactobacillus jinshanensis and Limosilactobacillus. We also found Actinobacteria in A. cerana and H. itama honey. This genomic data showed that A. cerana honey has a higher bacterial diversity than H. itama. Our finding is the first genomic study of bacterial diversity found in the honey of A. cerana and H. itama that live sympatrically in a bee farm.
Diversity of Actinomycetes from Soil Samples Collected from Lombok Island, Indonesia Lisdiyanti, Puspita; Tamura, Tomohiko; Ratnakomala, Shanti; Ridwan, Roni; Kartina, Gina; Lestari, Yulin; Ando, Katsuhiko; Widyastuti, Yantyati
Annales Bogorienses Vol. 16 No. 1 (2012): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A total of 137 strains of Actinomycetes were isolated from 14 soil samples collected in 3 part of Lombok Island, Indonesia using SDS-YE (SY) and Rehydration and Centrifugation (RC) isolation methods and Humic Acid Vitamin (HV) agar as isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. On the basis of their morphology and 16S rRNA gene sequence, 67% of isolates were belonged to the Streptomyces Group and 33% of isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Nine families and 15 genera were found from the samples. It is indicated the richness of actinomycetes in these area. The genus Streptomyces is the most abundant in all soil samples, occupying 67% of all isolates by using SY method; while the genus Actinoplanes is mainly found by using RC as isolation method. Two strains belong to the genus Virgisporangium and Catenulispora that showed taxonomic interest need further study for describing as ne species.
Diversity of Actinomycetes From Eka Karya Botanical Garden, Bali Ratnakomala, Shanti; Lisdiyanti, Puspita; Prayitno, Nita Rosalinda; Triana, Evi; Lestari, Yulin; Hastuti, Ratih Dwi; Widyastuti, Yantyati; Otoguro, Misa; Ando, Katsuhiko; Sukara, Endang
BIOTROPIA Vol. 23 No. 1 (2016): BIOTROPIA Vol. 23 No. 1 June 2016
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.4 KB) | DOI: 10.11598/btb.2016.23.1.504

Abstract

A total of 29 actinomycetes strains were isolated and identified by full sequence analysis of the 16S rRNA gene. Samples were collected from Bali Island, Indonesia, including 18 soil and 20 leaf litter samples from Eka Karya Botanical Garden. Two isolation methods were used in this study: SDS-extract (SY) and Rehydration-Centrifugation (RC). Based on 16S rRNA gene analysis, the isolated actinomycetes were grouped into two genera. Molecular analysis of 16S rRNA gene similarities showed that the actinomycetes isolated from Eka Karya Botanical Garden were of diverse origin. Analysis of soil samples resulted in 144 isolates belonging to 24 genera and more than 87 species, with Streptomyces being the dominant genus. This was followed by Actinoplanes (25 isolates, 17%). From leaf litter samples, 85 isolates were obtained, belonging to 9 genera and more than 41 species. The most dominant genus was Catenuloplanes (42 isolates, 49%), followed by Actinoplanes (16 isolates, 19%).
Co-Authors A. HAITAMI Abdjad Asih Nawangsih Alifian Ariansyah Amor Tresna Karyawati, Amor Tresna Anas Dinurrohman Susila ANDO KATSUHIKO Ando Katsuhiko Anja Meryandini Anja Meryandini Anja Meryandini Antonius Suwanto ARI SUSILOWATI Ariansyah, Alifian Aris Tri Wahyudi ARTINI PANGASTUTI Arum Asiyanti Suhastyo, Arum Asiyanti Arum Asriyanti Suhastyo BAMBANG MARWOTO Batara, Lily Noviani Budi Wirawan Deden Saprudin Dedy Duryadi Solihin Didy Sopandie DITA, SASMIATI FARACH DWI ANDREAS SANTOSA Dyah Iswantini DYAH NOOR HIDAYATI Efendi, Firda Sri Egra, Saat Elisa Sopiatul Fitriani Endang Sukara Evi Triana EVI TRIANA Farhana Halimah Rusyda Ferry Mutia Fitriandini, Risa GINA KARTINA Gina Kartina GINA KARTINA Hamim Hamim Hastuti, Ratih Dwi HIDEKI YAMAMURA Iman Rusmana Indah Puspita SARI Irmanida Batubara Isra Janatiningrum Iswandi Anas Iswandi Anas KATSUHIKO ANDO KATSUHIKO ANDO Laksmita Prima SANTI Lenni Fitri Lenni Fitri Lia Aseptin Murdini Lilis Nuraida Maggy T. Suhartono Maggy Thenawidjaja Suhartono Mahyarudin, Mahyarudin Mazidah Noer Inayah MISA OTOGURO MISA OTOGURO MONA PRIMANITA Munif Ghulamahdi N. Nurjanah Niken Financia Gusmawati NITA ROSALINDA PRAYITNO NITA ROSALINDA PRAYITNO Nunuk Widhyastuti NUNUK WIDHYASTUTI NUNUK WIDHYASTUTI PUSPITA LISDIYANTI Puspita Lisdiyanti PUSPITA LISDIYANTI Rahminiwati, Min RASTI SARASWATI RASTI SARASWATI RATIH DEWI HASTUTI RATIH DEWI HASTUTI Ratte, Marina RIKA RAFFIUDIN Risa Fitriandini ROHMATUSSOLIHAT ROHMATUSSOLIHAT RONI RIDWAN Roni Ridwan RONI RIDWAN Saat Egra SHANTI RATNAKOMALA Shanti Ratnakomala SHANTI RATNAKOMALA SHINJI MIYADOH Sipriyadi Sipriyadi Sipriyadi Sri Budiarti Sri Budiarti Poerwanto Suryo Wiyono SUSILOWATI1 SUSILOWATI1 TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Titi Candra Sunarti TOMOHIKO TAMURA Tomohiko Tamura TOMOHIKO TAMURA TRI RATNA SULISTIYANI TRIO HENDARWIN Umi Fatmawati Winanda, Witri Witri Winanda Yantyati Widyastuti Yantyati Widyastuti YANTYATI WIDYASTUTI Zaenal Abidin