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Bioactivity Test of Gambir (Uncaria gambir Roxb) Processing Waste as an Environmentally Friendly Alternative for Pest Control Using Nano Technology Hamid, Hasmiandy; Reflinaldon, Reflinaldon; Hidrayani, Hidrayani; Yunisman, Yunisman; Lina, Eka Candra
AGRIVITA Journal of Agricultural Science Vol 46, No 2 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i2.4397

Abstract

The gambir plant can serve as a raw material for botanical insecticides. Plant-based insecticides are made in nanoemulsion form to overcome the insecticide particle size problem. This study aims to obtain a gambir waste form that has the potential to be used as a botanical insecticide using nanotechnology. The laboratory experiments include preliminary and follow-up tests. The preliminary test involves testing each extract (liquid, solid, and raw gambir waste) at three concentrations using a completely randomized design with four replications. The follow-up test uses the residue method on leaves to test insecticidal activity. Creating nanoemulsion from gambir waste involves mixing organic and liquid phases through spontaneous emulsification. The insecticidal effectiveness of this nanoemulsion is tested on Croccidolomia pavonana. The results show that solid and liquid gambir waste has the potential to be used as alternative insecticides, which can influence the mortality of C. pavonana with an LC50 concentration of 0.22 (solid waste) and 0. 29 (liquid waste), while LC95 is 2.44 (solid waste) and 2.52 (liquid waste). The research promotes utilizing natural resources and innovative technologies, advancing environmentally conscious pest control methods, and fostering sustainable agricultural systems.
Aktivitas Insektisida Campuran Ekstrak Air Buah Piper aduncum L. (Piperaceae) dan Batang Cymbopogon ciratrus (Dc.) Stapf (Poaceae) Terhadap Larva Crocidolomia pavonana F. (Lepidoptera: Crambidae) Lina, Eka Candra; Supriadi, Adventus; Yunisman, Yunisman; Martinius, Martinius
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 1 No. 1 (2017): June 2017
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.1.1.34-41.2017

Abstract

Crocidolomia pavonana is an important pest in Brassicaceae. Botanical insecticides is pest control alternative which meet to eco-friendly manajement. This study aims to determine lethal concentration of single and mixture of water extract of Piper aduncum (Piperaceae) fruit and Cymbopogon cirtatus (Poaceae) stem against Crocidolomia pavonana larvae. The study was conducted in laboratory experiment through preliminary test and advance test using a completely randomized design (CRD). Observations were included to larval mortality, antifeedant effect, and duration of larval development. Bioassay was done against second instar larvae of C. pavonana using leaves immersion method during 48 hours. The results show that LC50 and LC95 value of mixture extract are 2.83% and 5.79% respectively. Based on index combination analysis, P. aduncum and C. citratus mixture extract were antagonistic at LC50 and additive at LC95 . Water extract of P. aduncum and C. citratus were relatively weak to influence feeding activity of C. pavonana larvae (41%). Mixture extract also extend larval development from second instar to third instar around 0.24 days and third instar to fourth instar around 0.97 days.
Aktivitas Insektisida Ekstrak Air Campuran Buah Piper aduncum dan Daun Tephrosia vogelii terhadap Crocidolomia pavonana Fabricius (Lepidoptera: Crambidae): english Afriyanita, Afriyanita; Lina, Eka Candra; Darnetty, Darnetty
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 3 No. 1 (2019): June 2019
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.3.1.34-46.2019

Abstract

The botanical insecticide is alternative pest control that is feasible to be developed. The inflorescences extract of Piper aduncum and leaf extract of Tephrosia vogelii are known to have insecticidal activity. The study aimed to determine the effect of a mixture of water extract of P. aduncum inflorescences and T. vogelii leaves on C. pavonana. This test used a completely randomized design (CRD) consisting of 6 treatments (0.0, 2.5, 3.0, 3.5, 4.0 and 5.0%) and 5 replications. Observation parameters were larval mortality, antifeedant effect, larval development time, pupal development time, normal and abnormal pupae, and sex ratio. The mixture of water extract of P. aduncum inflorescences and T. vogelii leaves (2:1) at LC50 (3.19%) was antagonistic and at LC95 (6.07%) was additive. The mixture influenced larval mortality (81.0%), had antifeedant effect (84.5%) and prolonged larval development time 1.71 days on 2nd-3rd instars and 2.4 days on 2nd-4th instars compared to control, but there were no effect on pupal development time, number of abnormal pupae and sex ratio.
Downstreaming nanoemulsion-based botanical insecticides: Enhancing competitiveness and sustainability of organic horticulture for KWT Kayu Gadang Tama, Duma Putri; Erona, Meisilva; Oktavia, Fitriyeni; Lina, Eka Candra
Community Empowerment Vol 10 No 12 (2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.15097

Abstract

Dependency on chemical pesticides is a major hurdle in achieving competitive and safe organic horticultural cultivation. This community service program aims to implement nanoemulsion-based botanical insecticide innovation as an eco-friendly pest control solution for the Kayu Gadang Women Farmer Group (KWT) in Sawahlunto. The methodology employed a participatory approach involving technology transfer, digital financial management mentoring, and product branding strategies. Evaluation results demonstrated significant impacts across three key pillars: an 80% reduction in chemical pesticide use per planting season, improved quality of residue-free horticultural products, and a 25% increase in business turnover. Additionally, marketing effectiveness rose, with sales volume growing by 50%. This program successfully transformed KWT Kayu Gadang into a self-sufficient and competitive horticultural business unit that supports green technology-based sustainable agriculture.
Effect of Defoamer of Botanical Insecticide Nanoemulsion Paramentandiol to Control Spodoptera frugiperda (Lepidoptera: Noctuidae) Fadhlurrahman, Rayhan; Lina, Eka Candra; Nelly, Novri
AGRIVITA Journal of Agricultural Science Vol 48, No 2 (2026)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.4779

Abstract

Formulating a stable para-menthane-3,8-diol (PMD) nanoemulsion requires a balanced ratio of active ingredients and surfactants. Increasing the active ingredient may improve efficacy but can reduce stability, while excessive surfactant levels may cause unwanted foaming during application. This study evaluates the use of a defoamer to improve nanoparticle stability and enhance the biological activity of the formulations against Spodoptera frugiperda. The research was conducted in two stages: (1) characterization of particle stability using the DLS method and (2) evaluation of insecticidal activity through the leaf-dip bioassay. Data were analyzed using ANOVA followed by the LSD test; LC50 and LC95 values were determined via Probit analysis using POLO PLUS. The addition of a defoamer significantly improved the physicochemical properties and performance of the PMD nanoemulsion. The formulation achieved a refined particle size of 202.2 nm and a PDI of 0.20, both within acceptable nanoparticle standards. At a concentration of 0.25%, the defoamer-enhanced formulation demonstrated superior biological efficacy, including a 0.07% increase in LC95 efficiency and 3.14% growth inhibition. The treatment reduced pupal formation and adult emergence by 8% and altered the sex ratio. The addition of a defoamer improves the physical properties of the nanoemulsion and its biological efficacy against Spodoptera frugiperda.
Sosialisasi Inovasi Insektisida Botani Menggunakan Teknologi Nanobubbles Sebagai Pengendali Hama Tanaman Hortikultura Duma Putri Tama; Varella Zahra; Zahlul Ikhsan; Lailatun Najmi; Novri Nelly; Eka Candra Lina
Buletin Dharmas Andalas Vol. 1 No. 2 (2024): Buletin Dharmas Andalas
Publisher : Departemen Budidaya Tanaman Perkebunan, Fakultas Pertanian, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bda.v1i2.17

Abstract

Pertanian modern saat ini dihadapkan pada berbagai tantangan yang memerlukan solusi inovatif dan berkelanjutan. Nanobubbles, sebagai partikel berukuran nano yang terperangkap dalam cairan, menawarkan potensi unik dalam pengembangan insektisida Nabati. Tujuan dilaksanakannya kegiatan pengabdian untuk meningkatkan pemahaman petani hortikultura khusnya daerah Solok, Sumatera Barat mengenai penggunaan insektisida botani berbahan aktif Tephrosia vogelii yang dipadukan dengan teknologi nanobubbles sebagai metode pengendalian hama yang ramah lingkungan. Sosialisasi dilaksanakan dengan metode ceramah atau persentasi berupa pemberian materi terkait inovasi dan pemaparan hasil penelitian yang telah berhasil dilakukan. Disamping itu juga dilakukan demonstrasi lapangan, dan diskusi interaktif. Hasil kegiatan ini adalah peningkatan pengetahuan dan keterampilan petani dalam memanfaatkan insektisida berbasis botani dengan efisiensi lebih tinggi. Implementasi teknologi nanobubbles dalam inovasi terbukti meningkatkan penetrasi dan distribusi bahan aktif sehingga efektivitas pengendalian hama menjadi lebih optimal.
The Potential of Refugia Plants in Increasing Arthropoda Diversity of Rice in South Solok Regency Yunisman, Yunisman; Annisa, Annisa; Hartatiana, Jenny; Syahrawati, My; Busniah, Munzir; Hamid, Hasmiandy; Lina, Eka Candra
CROPSAVER Vol 9, No 1 (2026)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v9i1.68108

Abstract

The conservation of natural enemies through the use of refugia plants is an important strategy to enhance arthropod diversity and stability in rice ecosystems, but its effectiveness may vary across locations. This study aimed to evaluate the potential of several refugia plant species in increasing arthropod diversity in rice fields of South Solok Regency, Indonesia. The study was conducted from December 2024 to April 2025 in Nagari Lubuk Gadang, South Solok Regency. Arthropod sampling was performed using a D-VAC (vacuum sampler) and insect net. The experiment was arranged in a randomized block design with six treatments (Cosmos caudatus, Ageratum conyzoides, Portulaca oleracea, Celosia cristata, Tagetes erecta, control), in four replications respectively. Observations included species composition, abundance, and diversity indices. The results showed that refugia plants contributed to the suppression of pest populations and improved the stability of arthropod communities. A total of 9 orders, 32 families, and 44 species were recorded, comprising 1,167 individuals, with herbivores accounting for 71.55% of the total population. The dominant herbivores were Cicadulina bipunctata, Leptocorisa oratorius, and Nilaparvata lugens, which were generally more abundant in rice fields without refugia. Predator and parasitoid abundance tended to be higher in the rice fields surrounded by C. cristata and T. erecta. The highest arthropod diversity was recorded in plots with T. erecta (H’ = 3.02), low dominance (0.07) and high evenness (0.86). Therefore, C. cristata and T. erecta are recommended as refugia plants to support sustainable integrated pest management in rice fields of mid-altitude areas.
Effectiveness of a nanoemulsion formulation of Piper aduncum L. extract and Cymbopogon nardus L. hydrosol in controlling the main pests of broccoli: Efektivitas formulasi nanoemulsi ekstrak Piper aduncum L. dan hidrosol Cymbopogon nardus L. untuk pengendalian hama utama tanaman brokoli Eka Candra Lina; Arneti; Joko Prasetyo
Jurnal Entomologi Indonesia Vol 23 No 1 (2026): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.23.1.30

Abstract

A nanoemulsion formulation of a mixture of Piper aduncum extract and Cymbopogon nardus hydrosol was evaluated for its effectiveness against the major broccoli pests, Crocidolomia pavonana and Plutella xylostella. The study was conducted in Alahan Panjang, West Sumatra, using a completely randomized design with four treatments (the nanoemulsion formulation, Bacillus thuringiensis, cypermethrin, and a control) and seven replications. Treatments were applied weekly from 21 to 70 days after planting (DAP). Larval population density and plant damage were recorded, and the data were analyzed using analysis of variance (ANOVA) followed by a least significant difference (LSD) test at a 5% significance level. Infestation by P. xylostella occurred earlier at 21 DAP, whereas infestation by C. pavonana began at 28 DAP. Overall, the population density of P. xylostella was higher than that of C. pavonana. The nanoemulsion formulation exhibited insecticidal activity, although its effectiveness was lower than that of cypermethrin and B. thuringiensis. The effectiveness rates against C. pavonana were 71.49%, 48.54%, 33.33%, and against P. xylostella were 73.49%, 60.88%, and 57.52% for cypermethrin, B. thuringiensis, and the nanoemulsion formulation, respectively.
Toxicity and Developmental Disruption of Piper aduncum Nanoemulsion against Paracoccus marginatus under Laboratory Conditions Mega Andini; Eka Candra Lina; Novri Nelly
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 1 (2025): June 2025
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.1.48-57.2025

Abstract

Paracoccus marginatus is one of the important pests that can reduce papaya production. Piper aduncum nanoemulsion has potential as a botanical pesticide to control papaya mealybug (Paracoccus marginatus). This present research was aimed to determine the most effective concentration in suppressing the development of P. marginatus. This study consisted of 6 treatments and 5 replications: 0.33%, 0.43%, 0.57%, 0.75%, 0.99%, and control. The observed parameters were: nymph mortality, adult mortality, daily mortality, adult emergence, LC50, and LC95. The data obtained were analyzed using variance (ANOVA) and 5% Tukey. The LC50, and LC95 value was analyzed using Polo Plus. Piper aduncum nanoemulsion exhibits strong insecticidal activity against nymphs and adults of Paracoccus marginatus under laboratory conditions. Among the concentrations tested, 0.99% was the most effective, causing 81.11% nymph mortality at 72 hours post-application; adult emergence was limited to only 13.33%. The LC₅₀ and LC₉₅ values at 72 hours were 0.506% and 1.222%, reflecting the potency of the nanoemulsion at relatively low doses. Further research under field conditions is recommended to evaluate its efficacy, formulation stability, and safety on non-target organisms.
Physiological Interference of Nanoemulsion-Formulated Botanical Insecticide Para-Menthane-3,8-Diol on Spodoptera frugiperda Rayhan Fadhlurrahman; Eka Candra Lina; Novri Nelly
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 2 (2025): December 2025
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.2.119-13-.2025

Abstract

Para-menthane-3,8-diol (PMD), derived from citronellal of citronella plants, has potential as an eco-friendly botanical insecticide for controlling Spodoptera frugiperda. This study aimed to evaluate the physiological interference caused by a PMD nanoemulsion formulation on S. frugiperda. Bioassays were conducted using the leaf-dip method. Data were analyzed using analysis of variance (ANOVA), followed by the Least Significant Difference (LSD) test with Statistix 8 and Duncan’s multiple range test at a 5% significance level using SPSS version 25. Probit analysis with POLO PLUS was performed to determine LC₂₅ and LC₅₀ values. The results showed that PMD nanoemulsion induced significant physiological interference in S. frugiperda, as indicated by increased mortality with LC₂₅ and LC₅₀ values of 0.15% and 0.48%, respectively. At a concentration of 0.75%, the treatment disrupted development by reducing pupation and adult emergence to 30% and altering the sex ratio to 1 male: 0.22 female. Sublethal interference was evident through strong antifeedant activity (62.88%) and pronounced changes in physiological parameters, including consumption rate (0.0328 mg/day), growth rate (0.0023 mg/day), and efficiency of ingested food (6.0029 mg/day). Overall, the PMD nanoemulsion formulation interfered with survival, feeding behavior, growth, and developmental physiology of S. frugiperda, highlighting its potential as an eco-friendly botanical insecticide.
Co-Authors . Reflinaldon Adventus Supriadi Afriyanita Afriyanita Afriyanita, Afriyanita Amalina Wahyuni Angga Ferdiyansyah Angga Ferdiyansyah Annisa Annisa Apdi Masela Mirsal Ardi Ardi Armansyah Armansyah Arneti Arneti Astri Harnov Putri Berri Brilliant Albar Caesar Welya Refdi Caesar Welya Refdi Cesar Welya Refdi DADANG DADANG Darnetty Darnetty Darnetty, Darnetty DJOKO PRIJONO Donard Games Duma Putri Tama Duma Putri Tama Dwi Monica Widya Sari Egi Adha Juniawan Ernis, Gustria Fadhlurrahman, Rayhan Hanalde Andre Hartatiana, Jenny Hasmiandy Hamid Hidrayani Hidrayani Indra Dwipa Joko Prasetyo Joko Prasetyo Joko Prasetyo Juniawan, Egi Adha Khairi, Khairi Lailatun Najmi Lailatun Najmi Ly Lan Phuong Martinius Martinius Martinius Martinius Mega Andini Meisilva Erona Sitepu MONA KAIRUNISA Muhammad Makky Muhammad Yasar Munzir Busniah Mustaqimah Mustaqimah Nia Yulianti NOVRI NELLY Obel, Obel Oktavia, Fitriyeni Prima Fithri Puti Afra Zenri Utami Putri Tama, Duma Rachmad Hersi Martinsyah Raida Agustina Ramadhan, Nugraha Rayhan Fadhlurrahman Rayhan Fadhlurrahman Rayhan Fadhlurrahman Rina Novia Yanti Ronauli Fernandes S RUSDI RUSLI Rusdi Rusli, Rusdi RUSLI RUSTAM Rusli Rustam Rusli Rustam Sang Phước Nguyễn Silmi Silmi Supriadi, Adventus Supriadi, Adventus SYAHRAWATI, MY Tama, Duma Putri Trisna Ayu Wandira Uyung Gatot Syafrawi Dinata Valeny Surya Ningsih Valeny Suryaningsih Varella Zahra Varella Zahra Welya Refdi, Cesar Wenny Surya Murtius Wenny Surya Murtius Yaherwandi Yaherwandi Yelsi Dwi Putri Yesi Puspita, Yesi Yola Octaviani Asri Yulianita Leoni Yulmira Yanti Yumhi Yumhi Yumhi Yunisman Yunisman YUNISMAN . YUNISMAN YUNISMAN Yunisman Yunisman Yunisman Yunisman Yunisman, Yunisman Yunisman, Yunisman Yusup Junaedi Zahlul Ikhsan Zahra, Varella Zaini Zaini