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All Journal Jurnal Ilmu Dasar AL KAUNIYAH Indonesian Journal of Cancer Chemoprevention UNEJ e-Proceeding Jurnal ORTOPEDAGOGIA BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) NurseLine Journal Jurnal SOLMA Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat Livestock and Animal Research EduInovasi: Journal of Basic Educational Studies Jurnal Kesehatan Masyarakat
Kiky Martha Ariesaka
Universitas Negeri Malang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Education Environmental Science Health Professions Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Expression of gonadotropin-releasing hormone receptor type-II correlates with proliferation activity in tissue microarray of rare ovarian tumor Ariesaka, Kiky Martha; Kusumanto, Ardhanu; Zucha, Muhammad Ary; Anggorowati, Nungki; Wicaksono, Agil Wahyu; Yunus, Moch; Fanani, Erianto; Nuryady, Moh Mirza
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.6034

Abstract

Activation of Gonadotropin-Releasing Hormone type II Receptors (GnRHR-II) exhibits antiproliferative activity. GnRHR-II is not only expressed exclusively in the pituitary, but also in a variety of tumors. To date, the clinical relevance of GnRHR-II in ovarian tumors is unclear. In addition, there is a lack of literature addressing GnRHR-II in ovarian tumors, especially rare types. This study was conducted to investigare the correlation between GnRHR-II expression with clinicopathology and proliferative activity of rare ovarian tumors. The purpose of this study was an analytic observational study with a cross-sectional design that utilized 18 ovarian tumor samples on tissue microarray (TMA). The expression of GnRHR-II and Ki67 was assessed using immunohistochemical staining (IHC) and observed using the IHC profiler plugin ImageJ software to obtain their respective H-scores. The data was analyzed using independent t-test, ANOVA, Pearson's test, and Fisher's exact test based on data types. The value of p<0.05 was considered statistically significant. GnRHR-II is expressed in various forms in ovarian tumors, including extrapituitary expression. GnRHR-II expression was highest in the sex cord stromal tumor (SCST) group, 110.30 ± 23.89 (p<0.0001). In addition, there was also a significant difference between GnRHR-II expression with age (p<0.001) and the primary tumor (p<0.05), but not with tumor type (p=0.101). There is a correlation between GnRHR-II expression and proliferative activity (r=-0.043, p=0.866). Elevated GnRHR-II expression is significantly correlation with SCST, individuals over 40 years of age, and tumors confined to the ovary and it is correlates with lower proliferative activity, although this correlation is very weak.
Enhancement of Teachers' Knowledge in Gamification for Improving Reading Skills in Students with Autism Spectrum Disorder Kurniawan, Agung; Ariesaka, Kiky Martha; Fukata, Editya; Wijaya, Andreas Budi; Putri, Angelica Igsanti; Prasetya, Ahmada Viosepta; Devi, Athaayaa Nastiti Ulayya
Jurnal ORTOPEDAGOGIA Vol 10, No 2 (2024): November
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um031v10i22024p75-82

Abstract

Autism Spectrum Disorder (ASD) is a developmental disorder characterized by challenges with social interaction, communication, and repetitive behaviors. Students with ASD often struggle with reading skills due to deficits in language comprehension, attention, and social communication. Gamification, applying game-design elements in non-game contexts, has emerged as a promising approach to enhance learning for these students. This study aimed to develop and implement a gamification-based learning application to improve the reading skills of students with ASD in special schools. The initiative sought to empower teachers through comprehensive training and support, enabling them to effectively use gamified educational tools. The method followed the ADDIE model: Analysis involved a needs assessment; Design included creating a training program and evaluation instruments; Development involved creating training materials and preparing for pilot testing; Implementation included conducting the training for teacher and administering pre-tests and post-tests; Evaluation consisted of analyzing test data and feedback. The training session was conducted on July 17, 2024, at SLB Autis Lab UM. Paired t-test results showed a significant increase in mean scores from pre-test (78.67±11.87) to post-test (88.00±10.14), with a P-value of 0.014, indicating effective training. Participant feedback highlighted the gamification application’s appealing interface, ease of use, and positive impact on student motivation, participation, and reading skills. The gamification-based learning application effectively improves reading skills in students with ASD and enhances teachers’ knowledge and skills in using such tools. Ongoing support and professional development for educators are crucial for integrating innovative tools into teaching practices, fostering academic success and overall development for students with ASD.
IN SILICO AND IN VITRO ANALYSIS OF BLASHV AND BLATEM GENE PRIMERS Khoiro, Aisha Azaria Nisa’ul; Purwanti, Elly; Miharja, Fuad Jaya; Ariesaka, Kiky Martha; Nuryady, Moh. Mirza
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 11 No. 2 (2025): Biolink February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v11i2.13830

Abstract

Bacterial pollution that occurs in the environment causes an increase in disease and antibiotic use, which leads to resistance. This study analyzes antibiotic-resistant blaSHV and blaTEM genes in silico and in vitro. Antibiotic-resistant bacterial isolates on ESBL will be carried out PCR and electrophoresis. The type of research used is descriptive observational research conducted from April to September 2024. The sampling technique used was random sampling. Primer blaSHV with sequence F' AGGATTGACTGCCTTTTTG and R' ATTTGCTGATTTCGCTCG, while primer blaTEM has sequence F' ATCAGCAATAAACCAGC and R' CCCCGAAGAACGTTTTC. Data analysis was carried out by analyzing in silico results obtained from the NCBI website and observing the visualization results of PCR amplification indicated by the presence of DNA bands.  In silico results showed 25 organisms attached to blaSHV and 29 organisms to blaTEM. In vitro results shown by electrophoresis visualization showed blaSHV amplicons measuring 392 bp with an annealing temperature of 50.5°C, and blaTEM measuring 517 bp with an annealing temperature of 42°C. The success rate of PCR was shown by clear and specific amplification of both genes, indicating that the PCR method performed was effective in detecting blaSHV and blaTEM genes in ESBL antibiotic-resistant bacterial isolate samples.  
Penyuluhan dan Pemeriksaan Kesehatan serta Pengobatan Gratis pada Masyarakat Desa Sindurejo Kecamatan Gedangan Kabupaten Malang untuk Skrining Penyakit Tidak Menular Ariesaka, Kiky Martha; Iftitahurroza, Hilma Tsurayya; Nuryady, Moh Mirza
Jurnal SOLMA Vol. 14 No. 1 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i1.15729

Abstract

Latar Belakang: Kesehatan merupakan aspek mendasar bagi produktivitas dan kualitas hidup manusia. Hingga saat ini, penyakit tidak menular (PTM) masih menjadi perhatian global, termasuk di Indonesia. PTM, seperti kardiovaskular, diabetes, dan kanker, menyebabkan lebih dari 70% kematian global, dengan tingkat kematian tertinggi di negara berkembang. Di Indonesia, prevalensi diabetes dan hipertensi meningkat, khususnya di wilayah pedesaan. Berdasarkan hasil wawancara dengan pemangku kebijakan setempat, Desa Sindurejo, Kab. Malang menghadapi tantangan deteksi dan penanganan PTM karena jarak yang signifikan  dari pusat layanan kesehatan. Kegiatan pengabdian masyarakat ini bertujuan untuk menjawab permasalahan deteksi PTM tersebut  dengan melakukan skrining dan penyuluhan kesehatan di Desa Sindurejo. Metode: Kegiatan ini melibatkan mitra yaitu kepala desa Sindurejo dengan target sasaran seluruh warga lansia di desa Sindurejo. Metode melibatkan pemeriksaan kesehatan yang terdiri atas pengukuran berat badan, tanda-tanda vital (tekanan darah, laju denyut nadi, frekuensi napas, dan suhu tubuh), dan pengukuran kadar gula darah sewaktu (GDS), asam urat dan kolesterol, serta penyuluhan dengan metode ceramah. Hasil: Hasil kegiatan yang dilaksanakan pada Januari 2024 di Desa Sindurejo menunjukkan antusiasme partisipasi warga, diikuti oleh total 46 orang lansia. Kegiatan skrining dan pemeriksaan gratis berhasil mendiagnosis PTM pada sebagian besar individu, sementara penyuluhan meningkatkan kesadaran akan pentingnya pencegahan PTM. Kesimpulan: Kesimpulannya, kegiatan ini memberikan dampak positif pada kesehatan masyarakat pedesaan dan mendukung upaya global mengurangi beban PTM melalui deteksi dini dan modifikasi gaya hidup sehat
Breeding Site Preferences and Resistance Status of Aedes aegypti in Malang City Nuryady, Moh Mirza; Purwanti, Elly; Permana, Tutut Indria; Ariesaka, Kiky Martha
Jurnal Kesehatan Masyarakat Vol. 21 No. 2 (2025)
Publisher : Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v21i2.21666

Abstract

Dengue Hemorrhagic Fever (DHF) remains a significant health challenge in urban and semi-urban regions, including Malang City, Indonesia, where Aedes aegypti is the primary vector. This study aimed to identify the breeding site preferences of Ae. aegypti and assess its resistance to 0.8% malathion and 0.05% cypermethrin insecticides. Using a descriptive observational design, ovitraps were deployed in three districts to collect mosquito larvae and eggs, and interviews with residents provided additional data on breeding site preferences. Resistance tests followed WHO guidelines, with mortality rates analysed after insecticide exposure. Results indicated that Ae. aegypti larvae were predominantly found in bathroom water tanks (45%) and flower vases (35%). Resistance status revealed geographical variability: Ae. aegypti in District 1 were resistant to cypermethrin, while populations in Districts 2 and 3 were susceptible, with average status of Ae. Aegypti to cypermethrin is tolerant. For malathion, resistance was widespread, particularly in District 3, with average status of Ae. Aegypti to malathion is resistant. These findings suggest that the use of malathion for vector control in Malang is no longer effective, while cypermethrin remains viable under strict monitoring to prevent future resistance. This study underscores the need for targeted insecticide use and regular monitoring to optimize vector control strategies and minimize DHF transmission.
Co-Authors Agil Wahyu Wicaksono Agung Kurniawan Ain Yuanita Insani Ain Yuanita Insani Al Munawir Anditri Weningtyas Andreas Budi Wijaya Ardhanu Kusumanto Arif Ladika Azka Darajat Brilliant Givya Ariansari Brilliant Givya Ariansari Devi, Athaayaa Nastiti Ulayya Diani Fatmawati Editya Fukata Elly Purwanti Erfan Efendi Fanani, Erianto Fannia Yosa Bakhtiar Faridatus Solikha Fatmawati, Diani Fuad Adi Prasetyo Fuad Adi Prasetyo Fuad Jaya Miharja Fulviansyah, Zaidhan Lucano Ganes, Tegar Gelaner, Nauval Akhmadian Haryono, Putri Dwinita Hilda Khairinnisa Hilma Tsurayya Iftitahurroza Iftitahurroza, Hilma Tsurayya Ika Rahmawati Sutejo Ika Wahyuni Inggita Utami Juliana, Levina Imelda Khoiro, Aisha Azaria Nisa’ul Lintang Widya Sishartami Maliza, Rita Mochammad Yunus, Mochammad Moh Mirza Nuryady, Moh Mirza Moh. Mirza Nuryady Mudzakir Taufiqurrahman Mudzakkir Taufiqurrahman Mudzakkir Taufiqurrqhman Nanang Tri Wahyudi Nazila, Farah Nungki Anggorowati Prasetya, Ahmada Viosepta Purwanti, Elly Putri, Angelica Igsanti Siti Nur Aldina Sri Wahyuni Suhartanti, Ajeng Sri Susetyarini, Rr Eko Tutut Indria Permana Ulfa, Darlah Immaria Wahyu Dian Puspita Wahyuni, Sri Zakiyatul Khoiriyah Zucha, Muhammad Ary