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All Journal HAYATI Journal of Biosciences Jurnal Sain Veteriner VALENSI Science and Technology Indonesia Biosaintifika: Journal of Biology & Biology Education Jambura Journal of Chemistry JURNAL MEDIA KESEHATAN Makara Journal of Science Jurnal Kedokteran Hewan Berita Biologi
SILMI MARIYA
Primate Research Center, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Veterinary

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CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM WHITE ADIPOSE TISSUE OF Macaca fascicularis Subu Taopan, Harrold Halilintar MR; Annisa Dewi, Fitriya Nur; Mariya, Silmi; Darusman, Huda; Permanawati, Permanawati; Sajuthi, Dondin; Suparto, Irma Herawati
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.18203

Abstract

Mesenchymal stem cells (MSC) derived from white adipose tissue are considered to be an appropriate therapeutic candidate for various human diseases due to their natural ability to maintain homeostasis. They have also been considered for use as a therapeutic alternative due to their sufficient availability and ease of harvesting. MSC play an important role in the process of adipogenesis and energy metabolism that is directly related to the pathogenesis of various degenerative diseases and metabolic syndromes. The purpose of this study was to obtain MSC cultures from white adipose tissue biopsied from three adult males Macaca fascicularis and to evaluate these cultures qualitatively and quantitatively regarding their characteristics and ability to differentiate. Polymerase chain reaction (PCR) was used to confirm the expression of mesenchymal stem cell-specific gene markers, and a specific growth medium was used to differentiate these cells into adipocytes, chondrocytes, and osteocytes. The results obtained showed that the MSC culture from M. fascicularis WAT had a fibroblast-like morphology with a spindle shape. The MSC markers of CD73, CD90 and CD105, were also expressed. The MSC culture was also able to differentiate into adipocytes, chondrocytes, and osteocytes. Based on these results, white adipose tissue from Macaca fascicularis was found to have potential as a source of MSC.
Aktivitas Sitotoksik Ekstrak Keong Laut Matah Merah (Cerithidea obtusa) terhadap Sel Kanker Kolon WiDr Dewa, Wisnu Jaka; Handharyani, Ekowati; Purwaningsih, Sri; Mariya, Silmi
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.83921

Abstract

Based on data released by Globocan in 2020, the incidence of colorectal cancer is the fourth highest in Indonesia (8.6%) and the third in the world (10%). This disease is hard to treat because the available therapy is less effective. Therefore, it is necessary to develop effective alternative therapies, especially those originating from Indonesia's natural resources, easy to obtain and reproduce. This study aims to determine the potential of red eye snail extract as an anticancer through cytotoxicity tests with the MTT Assay method on colon cancer cells WiDr and DNA fragmentation tests with Hoescth staining. In this study, we used various concentrations of the red-eye snail extract to test with 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) and doxorubicin reagents as positive controls. Absorbance values were read using a microplate reader at a wavelength of 595 nm. The cell absorbance data was converted into cell viability and probit analyzed to obtain the IC50 value. The results showed that the higher the concentration of the extract caused a decrease in the number of WiDr cells and an increase in damage to the structure of WiDr cells. Based on the results of probit analysis, it was found that the IC50 value of the extract was 36.28 µg/mL or classified as moderate cytotoxicity. The DNA fragmentation test showed that at concentrations of 125 ppm and 62.5 ppm, it was able to provide an effect similar to doxorubicin, namely triggering apoptosis in WiDr colon cancer cells.
PENAPISAN MODEL HEWAN PRIMATA Macaca fascicularis UNTUK UJI VAKSIN Human Papilloma Virus Sari, Isti Kartika; Mariya, Silmi; Setyawaty, Dyah; Suparto, Irma H.; Mustopa, Apon Z.; Darusman, Huda Salahudin
Berita Biologi Vol 23 No 2 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.1052

Abstract

Pemanfaatan hewan primata dalam hal ini monyet ekor panjang (MEP) atau Macaca fascicularis sebagai hewan model untuk uji vaksin Human Papilloma Virus (HPV) harus melalui penapisan untuk mendapatkan hewan yang tepat. Tujuan dari penelitian ini untuk menapis dan memvalidasi kecocokan MEP sebagai hewan model vaksin papillomavirus. Jenis vaksin yang akan di uji menentukan jenis penapisan yang dilakukan. Metode penapisan dilakukan dengan uji serologis, molecular dan patologi serta validasi dengan histopatologi. Vaksin pencegahan atau profilaksis memerlukan hewan yang bebas dari virus-virus penekan kekebalan tubuh (immunosuppressive virus) dan HPV nya sendiri agar antibodi yang terbentuk benar-benar berasal dari vaksin yang diujikan, vaksin terapeutik memilih hewan dengan positif MfPV khususnya MfPV3. Hasil penapisan dari 136 ekor hewan diperoleh 42 (30%) ekor hewan dengan negatif antibodi serta  diperoleh  39 (28%) ekor MEP positif MfPV diantaranya yaitu sebanyak 24 (17%) ekor positif MfPV-3. Hewan dengan positif MfPV3 selanjutnya akan digunakan untuk uji vaksin terapeutik HPV. Adanya perubahan abnormal sel-sel epitel serviks pada hewan dengan positif MfPV yang menuju pada pembentukkan sel kanker (Carcinoma Intra epithelial Neoplasia/CIN) juga semakin menguatkan kemiripan model MEP dengan manusia.
Co-Authors Agus Setiyono Al Azhar Annisa Dewi, Fitriya Nur Arif Rakhman Hakim Bambang Pontjo Priosoeryanto Bintoro, Yudha Dayana, Hepy Deby Anggraini, Deby Dewa, Wisnu Jaka Dian Pratiwi Dondin Sajuthi Ekowati Handharyani Elok Budi Retnani Huda Shalahudin Darusman Ichsan Ichsan IETJE WIENTARSIH Indriawati, Iin Irma Herawati Suparto Irmanida Batubara Jusak Nugraha KARTINI ERIANI Krisnayanti, Ni Putu Eka Mia Audina Muhammad Hamdan Mulyatni, Agustin Sri Mustopa, Apon Z. Ningrum, Emilna Mega Noviana, Rachmitasari PERMANAWATI, PERMANAWATI Putri, Indah Aprianti Ratu, Safira Pinaka Pramestika Rayendra, Raendi Raymond R. Tjandrawinata ROHAYATI SUPRIHATINI Saifanah Setiawan, Nabilah Sari, Isti Kartika Sebayang, Shalina Setyawaty, Dyah Sri Purwaningsih Subu Taopan, Harrold Halilintar MR Suduri, Adam SUMINAR SETIATI ACHMADI Suparto, Irma H. UUS SAEPULOH Valentina Sokoastri Yuliana Yuliana Yusuf Ryadi Zaelani, Bella Fatima Dora