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All Journal Jurnal Teknologi Dan Industri Pangan EKSAKTA: Journal of Sciences and Data Analysis JURNAL KIMIA SAINS DAN APLIKASI Pharmaciana: Jurnal Kefarmasian Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Indonesian Journal of Cancer Chemoprevention Eksergi: Chemical Engineering Journal Jurnal Rekayasa Sipil dan Desain Jurnal Kimia dan Kemasan agriTECH Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Majalah Obat Tradisional EduChemia: Jurnal Kimia dan Pendidikan Jurnal Kimia Riset Dental Journal (Majalah Kedokteran Gigi) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Titian Ilmu: Jurnal Ilmiah Multi Sciences ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Jurnal Aplikasi Teknologi Pangan Berdikari: Jurnal Pengabdian Masyarakat Indonesia Indonesian Journal of Chemical Analysis (IJCA) JKPK (Jurnal Kimia dan Pendidikan Kimia) Jurnal Ilmiah Cendekia Eksakta Stannum : Jurnal Sains dan Terapan Kimia IJCR (Indonesian Journal of Chemical Research) STATIKA: Jurnal Teknik Sipil Magistrorum et Scholarium: Jurnal Pengabdian Masyarakat Gunung Djati Conference Series AGRIC Rafflesia Journal of Natural and Applied Sciences (RJNAS) Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Pharmacy and Chemistry
Yohanes Martono
Universitas Kristen Satya Wacana
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Validation of UV-VIS Spectrophotometric Methods for Determination of Inulin Levels from Lesser Yam (Dioscorea esculenta L.) Sandra Ayu Apriliyani; Yohanes Martono; Cucun Alep Riyanto; Mutmainah Mutmainah; Kusmita Kusmita
Jurnal Kimia Sains dan Aplikasi Vol 21, No 4 (2018): volume 21 Issue 4 Year 2018
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2211.725 KB) | DOI: 10.14710/jksa.21.4.161-165

Abstract

Validation of methods is necessary to ensure that the analytical methods used are appropriate and reliable. In this research, the determination of validation method and determination of inulin content from gembili bulb (Dioscorea esculenta L.). The method used for determining inulin levels is UV-Vis spectrophotometry. Based on the results obtained, the maximum wavelength determination of inulin levels is 520 nm. The method of analysis in this study meets the specified validation requirements. These parameters include the equation on the inulin raw curve y = 0.0045x +0.109 with the coefficient of determination 0.9992. The accuracy of the methods tested using the recovery test was 96.14-106.76% at concentrations of 25, 100, and 225 ppm. The intraday precision test is in the range of 2.67-6.82% at the same concentration as the accuracy test. Precision interday equal to 7.41% at concentration 225 ppm for 3 day analysis period. The values of LOD and LOQ are 3.58 ppm and 11.95 ppm, respectively. The concentration of inulin extract from gembili bulb is 840.44-12188.59 ppm.
Kinerja Karbon Aktif Daun Eceng Gondok pada Penurunan Kadar Fosfat Artifisial dan Surfaktan dalam Limbah Detergen Resa Wulandari; Cucun Alep Riyanto; Yohanes Martono
ALCHEMY Jurnal Penelitian Kimia Vol 19, No 2 (2023): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.19.2.65626.149-161

Abstract

Limbah detergen masuk ke dalam kategori limbah domestik karena limbah ini memiliki potensi membentuk film pada permukaan air dan menurunkan tingkat transfer oksigen ke dalam air. Oleh karena itu, penanganan limbah detergen diperlukan untuk meningkatkan kualitas air. Pada penelitian ini dilakukan sintesis karbon aktif dari Daun Eceng Gondok (DEG) sebagai adsorben fosfat dalam limbah fosfat artifisial dan surfaktan dalam limbah detergen. Tingginya kandungan selulosa dalam daun eceng gondok membuat tanaman tersebut memiliki potensi untuk diolah menjadi bahan dasar alternatif untuk karbon aktif. Sintesis karbon aktif DEG dilakukan melalui proses karbonisasi pada suhu 400 ℃ selama 60 menit dilanjutkan proses aktivasi dengan rasio karbon: H3PO4 30% yaitu 1:4 (b/b). Selanjutnya, pemanasan dilakukan menggunakan furnace pada suhu 600 ℃ selama 60 menit. Hasil penelitian menunjukkan bahwa karbon aktif DEG memiliki gugus fungsional O‒H, C‒H, C=C, C≡C, dan C‒O serta karakter mikropori (d = 1,10 nm) dan luas permukaan sebesar 726,49 m2/g. Pada kajian kinetika dan isoterm adsorpsi, penjerapan karbon aktif DEG terhadap fosfat dan surfaktan mengikuti pemodelan kinetika Elovich dengan kapasitas adsorpsi masing-masing adalah 11,67 mg/g dan 4,19 mg/g. Efektivitas karbon aktif DEG dalam adsorpsi fosfat dan surfaktan berturut-turut mencapai 78,35% dan 52,99%.Performance of Activated Carbon from Water Hyacinth Leaves (WHL) for Reducing Artificial Phosphate and Surfactant Levels in Detergent Waste. Detergent waste is classified into the domestic waste category because it forms a film on the water surface and reduce the oxygen level in the water. Therefore, handling detergent waste is necessary to improve water quality. In this research, activated carbon was synthesized from water hyacinth leaves (WHL) as an adsorbent for phosphate in artificial phosphate waste and surfactant in detergent waste. The high cellulose content in water hyacinth leaves gives this plant the potential to be processed into an alternative base material for activated carbon. The synthesis of activated carbon from WHL (ACWHL) was carried out through a carbonization process at 400 ℃ for 60 min followed by an activation process using a weight ratio of carbon: H3PO4 30% = 1:4 (w/w) followed by heating at 600 ℃ for 60 min. The research results show that ACWHL has the functional groups O‒H, C‒H, C=C, C≡C, and C‒O as well as microporous character (d = 1.10 nm) and a surface area of 726.49 m2/g. In the study of kinetics and adsorption isotherms, the adsorption of ACWHL on phosphate and surfactants followed Elovich kinetic modeling with adsorption capacities of 11.67 mg/g and 4.19 mg/g, respectively. The effectiveness of ACWHL in adsorption of phosphate and surfactant reached 78.35% and 52.99%, respectively.
Reducing Chemical Oxygen Demand and Turbidity Levels in Laundry Waste Using Activated Carbon from Water Hyacinth Leaves Cucun Alep Riyanto; Nur Ayu Hidayati; Yohanes Martono
Indonesian Journal of Chemical Analysis (IJCA) Vol. 6 No. 2 (2023): Indonesian Journal of Chemical Analysis
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijca.vol6.iss2.art8

Abstract

Lignocellulose compounds contained in water hyacinth leaves (WHL) (Eichhornia crassipes) can be used as raw material for the manufacture of activated carbon at a low and renewable rate. The ACWHL can be used as adsorbent for reducing a COD value and turbidity levels on laundry waste. The purpose of this study is to determine the best adsorption modelling of ACWHL on COD values and turbidity levels in laundry waste in kinetics and isotherm studies and determine the effectiveness of reducing a COD value and turbidity levels. The process of making ACWHL is carried out by carbonization at 400 °C for 1 hour, then activated using 30% H3PO4 at an impregnation ratio of 1:4 (w/w) for 24 hours and physical activation at 600 °C for 1 hour. The results showed that the adsorption of ACWHL on COD value and turbidity levels of laundry waste followed Freundlich isotherm and Elovich isotherm modelling with R2 values of 0.9535 and 0.9905, respectively. The modelling for adsorption kinetics of COD values and turbidity levels in laundry waste follows the Pseudo Second Order kinetics modelling. The effectiveness of ACWHL reduction in adsorption of COD value and turbidity levels of laundry waste was 80.76% and 64.71%.
PENGARUH INTENSITAS CAHAYA TERHADAP DEGRADASI WARNA AGARAGAR YANG DIWARNAI SARI UMBI BIT MERAH (Beta vulgaris L. var. rubra L.) Lydia Ninan Lestario; Noviana Gunawan; Yohanes Martono
Agric Vol. 25 No. 1 (2013)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2013.v25.i1.p42-50

Abstract

The aims of this research were to determine the concentration of beetroot juice in jelly which panelists like best; to determine the color degradation rate constant in jelly colored with beetroot juice caused by sunlight and fluorescent light; and color degradation rate constant of jelly colored with synthetic colorant.The organoleptic test used 2,5 percent; 5 percent; 7,5 percent; 10 percent; and 12,5 percent beetroot juice and permitted synthetic colorant for food. The light intensity to test the beetroot color stability in jelly were 879,06 lux, 1.688,54 lux, 2.342,2 lux and 6.752,2 lux.The results showed that the concentration of beetroot juice which panelists like best was 5 percent.; The color degradation rate constant of jelly colored by 5 percent beetroot juice on intensity 879,06 lux was 0,0468 hour-1; 1.688,54 lux was 0,0565 hour-1; 2.342,2 lux was 0,0653 hour-1;6.752,2 lux was 0,0887 hour-1. The higher the light intensity, the higher the degradation constant rate.
Combination of polyherbal Phyllanthus reticulatus with Zingiber officinale and Cymbopogon citratus to optimize the antioxidant capacity Elizabeth Betty Elok Kristiani; Sri Kasmiyati; Yohanes Martono
Pharmaciana Vol 14, No 1 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i1.28002

Abstract

Currently, the public is interested in polyherbal-based foods and beverages as a source of natural antioxidants. The aim of the study is to evaluate the antioxidant properties and the phenolic and flavonoid compounds of formulations containing Z. officinale, C. citratus, and P. reticulatus (ZCP). Each sample was extracted using the maceration process in an ethanol solvent at room temperature for three 72-hour periods. There were fourteenth formulation of Z. officinale rhizome, C. citratus leaves, and P. reticulatus fruit which used Design of Expert (DoE). The DPPH method was used to determine the power of antioxidants. The flavonoid content of the extract was measured using the colorimetric method and AlCl3 reagent, while phenolics content using Folin-Ciocalteu. The formulations ZCP 1:0:0, 0:0:1, and 1:1:1 showed the antioxidant capacity in a strong categorization, with an IC50 value less than 50 µg/ml, while ZCP 0:1:0 was in a weak categorization (IC50 > 250 µg/ml). Another ZCP formulation was in a medium category. The ZCP 1:1:1 formulation was suggested as the best one for this investigation, which contains three plant samples. This formulation is interesting for further toxicity studies and in vivo testing so that it can be applied as an antioxidant-rich supplement product.
Synthesis of porous Hydroxyapatite (HAp) from Laying Chicken Waste Eggshells Agung Rimayanto Gintu; Elizabeth Betty E. Kristiani; Yohanes Martono
RAFFLESIA JOURNAL OF NATURAL AND APPLIED SCIENCES Vol. 2 No. 2 (2022): Oktober
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/rjna.v2i2.23931

Abstract

Hydroxyapatite (HAp) was the type of bioceramics synthesized from the compound which rich Calcium (Ca) contains, these bioceramics commonly used to repair the damage of hard tissues. In this study threatened the synthesis of HAp from laying chicken eggshells, the characterization of the eggshells showed that the contains of Calcium 0,9809 ± 0,0118g/g; Phosphate 0,0236 ± 0,0009g/g; and ratio of Ca/P 2,0840%. The synthetic process by base precipitation method showed the yield 77,5786 ± 0,3509%; and from the synthetic obtained the HAp with contains of Calcium 0,1522 ± 0,0047g/g; Phosphate 0,01354 ± 0,0019g/g; and ratio of Ca/P 0,9162%. The Biochemical characterization of the HAp showed that the Porosity 70,5940 ± 0,4104%; Biodegradability 16,1879 ± 0,1362%; and swelling Ability 28,0549 ± 0,1054%. The entire result of the research concluded that the sample eggshells potentially became one of the sources of HAp, and the HAp synthesized from the eggshells potentially to apply as remineralization agents.
Leaf Extract Microencapsulation of Stevia rebaudiana Bert Using Inulin-Chitosan as Anti-Diabetes Diet Mutmainah Mutmainah; Yohanes Martono; Ika Puspitaningrum; Lia Kusmita
Journal of Tropical Pharmacy and Chemistry Vol. 5 No. 3 (2021): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v5i3.270

Abstract

Diabetes mellitus is a collection of symptoms that arise in someone who has increased blood glucose levels. The Stevia plant (Stevia rebaudiana Bert) contains a compound of diterpene glycosides as steviosida and rebaudiosida A. Purified extract of steviosida and rebaudiosida A is widely used as a sweetener for low calorie food and beverage products or as a sugar substitute for diabetics and has the effect of lowering blood sugar levels. This study aims to determine the antidiabetic effect of microencapsulated preparations of Stevia leaf extract (Stevia rebaudiana) with a combination of inulin chitosan encapsulation. Antidiabetic mellitus test was carried out in vivo using test animals of male white rats of Wistar strain. The inducing compound that can cause the condition of diabetes mellitus test animals is Aloxan with a dose of 150 mg / kg Body weight of rats. given intraperitoneally for one day, then the mice were allowed to stand for 3 days to reach a state of diabetes mellitus. Blood glucose levels of test animals were measured on days 1 (initial), 5 (induction) and 12 (treatment) to determine the initial blood glucose levels, after induction of alloxan and after administration of test compounds both CMC Na 0.5% , glibenclamide, and preparations microencapsulation of Stevia leaf extract at a dose of 100; 300; and 700 mg / kg body weight. The results were obtained after 7 days of treatment, it was seen that blood glucose levels in the CMC group remained high, while the Glibenclamid administration group, and the three dosages of microencapsulation preparations of Stevia leaf extract could reduce blood glucose levels. This can be seen from the statistical test that there is a significant difference (p
EFEKTIVITAS ASPAL EMULSI METODE CAMPURAN DINGIN PADA LAPISAN LASTON AC-BASE: EFEKTIVITAS ASPAL EMULSI METODE CAMPURAN DINGIN PADA LAPISAN LASTON AC-BASE Frederik Tambunan, Hermon; Martono, Yohanes; Catur Marina, Bernaditha; Dwiky Iswara, Kerri; Abi Berkah Nadi, Muhammad; Zhafira, Elian; Gunawan, Raden
STATIKA: Jurnal Teknik Sipil Vol. 10 No. 1 (2024): STATIKA JURNAL TEKNIK SIPIL
Publisher : Politeknik Raflesia Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53494/jts.v10i1.575

Abstract

Perancangan campuran aspal panas merupakan metode pencampuran umum yang diterpakan dalam pelaksanaan lapis perkerasan jalan. Tantangan suhu yang harus memenuhi spesifikasi teknis menjadi perhatian saat penghamparan. Penggunaan aspal emulsi untuk campuran spal dingin diharapkan mmpu menjadi solusi. Studi ini dilakukan untuk melihat efektifitas dari penggunaan aspal emulsi campuran dingin dibandingkan dengan aspal campuran panas pada jenis perkerasan Laston AC-Base. Hasil penelitian penelitian menunjukkan dengan campuran aspal emulsi sebesar KAE 7,3% memiliki nilai stabilitas mencapai 2.635 kg, namun memiliki angka flow sebesar 1,78 mm. Angka ini menunjukkan bahwa hasil campuran aspal dengan menggunakan aspal emulsi campuran dingin memiliki tingkat kelelehan plastis yang masih dibawah ambang batas minimal sebesar 3 mm.
Combination of polyherbal Phyllanthus reticulatus with Zingiber officinale and Cymbopogon citratus to optimize the antioxidant capacity Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Martono, Yohanes
Pharmaciana Vol. 14 No. 1 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i1.28002

Abstract

Currently, the public is interested in polyherbal-based foods and beverages as a source of natural antioxidants. The aim of the study is to evaluate the antioxidant properties and the phenolic and flavonoid compounds of formulations containing Z. officinale, C. citratus, and P. reticulatus (ZCP). Each sample was extracted using the maceration process in an ethanol solvent at room temperature for three 72-hour periods. There were fourteenth formulation of Z. officinale rhizome, C. citratus leaves, and P. reticulatus fruit which used Design of Expert (DoE). The DPPH method was used to determine the power of antioxidants. The flavonoid content of the extract was measured using the colorimetric method and AlCl3 reagent, while phenolics content using Folin-Ciocalteu. The formulations ZCP 1:0:0, 0:0:1, and 1:1:1 showed the antioxidant capacity in a strong categorization, with an IC50 value less than 50 µg/ml, while ZCP 0:1:0 was in a weak categorization (IC50 > 250 µg/ml). Another ZCP formulation was in a medium category. The ZCP 1:1:1 formulation was suggested as the best one for this investigation, which contains three plant samples. This formulation is interesting for further toxicity studies and in vivo testing so that it can be applied as an antioxidant-rich supplement product.
Extract Formulation of Zingiber officinale and Phylanthus reticulatus Induces Apoptosis of WiDr Colon Cancer Cells Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Martono, Yohanes; Kachingwe, Baxter Hepburn
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.25776

Abstract

People use ginger (Zingiber officinale) extensively for health purposes. Our previous studies have shown that Phyllanthus reticulatus has strong antioxidant capabilities. This study investigates the anticancer potential of ethanolic extract of Z. officinale and P. reticulatus formulations (ZP), focusing on their cytotoxicity and mechanisms against cancer cells. Samples were extracted using the maceration method. The method for determining the cytotoxicity of cells is MTT, the cell cycle is flow cytometry, apoptotic cells are flow cytometry and double staining, and caspase-8 and caspase-9 expression using the immunocytochemistry method. The analysis of caspase-8 and caspase-9 expression using the immunocytochemistry method. Among the tested formulations, the ethanolic extract of ZP_1:0 showed the most promise against WiDr colon cancer cells (IC50 value of 102.1 µg/ml). The ZP_1:0 demonstrated dose-dependent apoptosis induction in WiDr cells, showing early and late apoptosis, with minimal necrosis. The extract activates extrinsic and intrinsic apoptotic pathways in inducing cancer cell death. Compared to doxorubicin, the ZP 1:0 formulation selectively promotes apoptosis with reduced necrotic damage, suggesting the potential for reduced systemic toxicity. The study supports the potential of Z. officinale as a source of safer, plant-based anticancer agents, particularly for colon cancer, while recommending further research to identify and enhance active compounds.
Co-Authors A. Rindang Anggit Laksono Abdul Rohman Abi Berkah Nadi, Muhammad Agung Rimayanto Gintu Agung Rimayanto Gintu Agung Rimayanto Gintu Agung Rimayanto Gintu Agung Rimayanto Gintu Andreas Setiawan Andriyani Rosita Sari Anggelia Essi Christian Ari Hasna Widyapuspa Bereka Meidelivia Raharjianti Bernaditha Catur Marina Betsy Felita Betsy Felita Cucun Alep Riyanto Daniel Ricky Putra Wijaya Devina Intan Sari Devinta Lestari Dewi Kurnianingsih Arum Kusumahastuti Diyonisio Kevin Elisando Dwiky Iswara, Kerri Eliana Prabalaras Elisabeth Betty Elok Kristiani Elizabeth Betty E. Kristiani Elizabeth Betty Elok Kristiani Erlien Giovani Soeroso Fahmi Puteri Perdani Ferdy Semuel Rondonuwu Fidelia Novitasari Fransiskus Tri Wahyu Hananto Franyoto, Yuvianti Dwi Gunawan, Raden Harry Setiawan Hartati Soetjipto Hartati Soetjipto Hindra, Ronal Febria Ida Suskawati Ika Puspitaningrum Ika Puspitaningrum Ira Tyas Kurniasari Kachingwe, Baxter Hepburn Kiki Fransiska Suharto Kusmita Kusmita Lia Kusmita Lucia Devi Danriani Lydia Ninan Lestario Maria Endah Retno Palupi Muhamad Syaiful Ampri Mutmainah Mutmainah Mutmainah Mutmainah Mutmainah Mutmainah November Rianto Aminu Noviana Gunawan Nur Ayu Hidayati Rahayu Sulistyorini Rema Fitriyani Resa Wulandari Salsabila Eka Ghina Rana Sandra Ayu Apriliyani Sekar Nurani Saptaningtyas Sherly Novita Sari Nawawi Slamet Widodo Sri Hartini Sri Hartini Sri Kasmiyati Sudibyo Martono Sudibyo Martono Sugeng Riyanto Suryasatriya Trihandaru Susanti Pudji Hastuti Tambunan, Hermon Frederik TATI NURHAYATI Tiffany Febetania Widodo Tonia Nur Fitria Widhi Nugroho Meindra Wijaya, Daniel Ricky Putra Yayi Suryo Prabandari Yoko Putra Nusantara Yuniarti, Zulfa Zhafira, Elian Zulfa Yuniarti