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All Journal Jurnal Sain Veteriner Jurnal Pengendalian Hayati Berkala Ilmiah Pertanian Agrovigor: Jurnal Agroekoteknologi SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Ilmiah Inovasi AMALIAH: JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Pemberdayaan Masyarakat Universitas Al Azhar Indonesia Jurnal Bioindustri Jurnal Proteksi Tanaman Tropis J-PEN Borneo : Jurnal Ilmu Pertanian Journal of Applied Agricultural Science and Technology MIMBAR INTEGRITAS Biosaintifika: Journal of Biology & Biology Education Journal of Bioindustry
Rachmi Masnilah
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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ENCAPSULATION OF SOYBEAN SEEDS WITH Actinomycetes AS A BIOCONTROL AGENT AGAINST Sclerotium rolfsii Sacc Masnilah, Rachmi; Anam, Khairul; Pradana, Ankardiansyah Pandu
J-PEN Borneo : Jurnal Ilmu Pertanian Vol 7, No 1 (2024)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS BORNEO TARAKAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/jpen.v7i1.5270

Abstract

This study evaluates the effectiveness of Actinomycetes in encapsulating soybean seeds to control damping-off disease caused by Sclerotium rolfsii. With national soybean demand reaching 22 million tons per year and domestic production only 1 million tons, increasing production is essential. One major constraint is the attack of plant pathogens such as S. rolfsii, which can cause significant losses. Actinomycetes were isolated from the rhizosphere of weeds and tested for their antagonistic activity in vitro. Seed encapsulation using various carriers (kaolin, talc, and zeolite) was conducted to determine the most effective formula. In vivo tests showed that the Actinomycetes isolate PM2 exhibited the best antagonistic activity, with the zeolite formulation reducing pre-emergence damping-off disease incidence to 12.50% and post-emergence damping-off to 8.25%, compared to the positive control. These results indicate that the use of Actinomycetes in soybean seed encapsulation is an effective and sustainable method for controlling damping-off disease. Zeolite as a carrier proved to provide a supportive environment for antagonistic microbes, maintaining moisture and supplying necessary nutrients. This research reveals the great potential of Actinomycetes as a biocontrol agent in plant disease management strategies, which can significantly enhance soybean production and support national food security.
The Potential of Deleterious rhizobacteria to Control Primary Weed Echinochloa sp. on Rice Hoesain, Mohammad; Masnilah, Rachmi; Nurchayanti, Suhartiningsih Dwi; Pradana, Ankardiansyah Pandu; Alfarisy, Fariz Kustiawan; Sari, Dwi Tirta; Ramadhan, Farchan Mushal Al; Rismayani; Tarigan, Sri Ita
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.3422

Abstract

Deleterious rhizobacteria (DRB) is one of the rhizobacteria that can be used as a bioherbicide and can stimulate plant growth. The objectives that can be obtained from this study are to determine the potential of DRB on the growth of weeds Echinochloa crusgalli and Echinochloa colona on rice. Variables observed in this study were identification of secondary metabolites, measurement of weed sprouts, percentage effectiveness of bacterial isolates against weed seeds, normal sprouts, abnormal sprouts, fresh seeds that did not grow and dead seeds. The aim of this study is to explore biological agents that can be used to control the main weeds of rice crops. This candidate could be a recommendation for environmentally friendly controls. Exploration of the DRB group was obtained by collecting soil samples around the roots of rice crops. Then isolate it, identify as morphologically and physiologically, and test it in vitro studies. The results showed that DRB had the potential to control weed growth and could stimulate the growth of rice. The application of PFA and PFB isolates had a significant effect on inhibiting the length of weed roots and buds, the average percentage of control effectiveness > 80-90%. Novelty in this study could be found as candidate groups of DRB bacteria with specific locations to control the weed Echinochloa sp. on rice crops. For society, this research could be an alternative to reduce dependence on synthetic herbicides . DRB has the potential to be formulated into a bioherbicide  to control weeds.
EKSPLORASI Bacillus spp. PADA BEBERAPA RHIZOSFER GULMA DAN POTENSINYA SEBAGAI AGENS PENGENDALI HAYATI PATOGEN TANAMAN SECARA IN VITRO Rana Virga Tesha Syofiana; Rachmi Masnilah
JURNAL BIOINDUSTRI (JOURNAL OF BIOINDUSTRY) Jurnal Bioindustri: VOL. 2 NO. 1 (2019)
Publisher : Universitas Trilogi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31326/jbio.v2i1.483

Abstract

One group of rhizobacteria that has been widely studied as a biological controlling agent is Bacillus spp. Through the mechanism of induction of resistance and antibiosis, these bacteria are able to suppress the growth of plant pathogens. Its ability to quickly colonize plant roots and broad adaptability to the environment causes Bacillus to spread in nature, especially in the rhizosphere. This study aims to determine the presence of Bacillus in several rhizosphere weeds and their ability to inhibit plant pathogen growth in vitro. This study consisted of two stages, namely, (1) sampling activities carried out in the Kalisat area, Jember Regency, and (2) isolation, selection and identification. Based on the results of the study, 17 Bacillus spp. Isolates. which was successfully isolated from several rhizosphere weeds. The results of Bacillus spp. in vitro the inhibition was obtained by 73% in suppressing Fusarium sp. and 14 mm clear zone formed by Bacillus against X. oryzae pv. oryzae. 5 superior Bacillus isolates obtained from the antagonist test were identified as B. subtilis, B. licheniformis, B. alvei, and B. coagulans.Keyword: Bacillus spp., biological agents, weeds of rhizosphere
Program Konservasi Penanaman 1000 Pohon Berbasis Masyarakat di Wilayah Hulu DAS Bedadung Jember: Conservation Program for Planting 1000 Tress Based on Society in the Upstream Area of the Bedadung Jember Watershed Habriantono, Bakhroini; Alfarisy, Fariz Kustiawan; Suharto, Suharto; Wagiyana, Wagiyana; Nurcahyanti, Suhartiningsih Dwi; Masnilah, Rachmi; Hoesain, Mohammad; Nusantara, Alrio Putra; Hakim, Tiara Oktavia Putri; Lestari, Resti Putri; Risqianti, Riza; Fa'ayunina, Miftakhul; Merina, Gusna; Ramadhani, Farchan Mushaf Al; Putra, Dimas Ganda Permana
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 11 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i11.7794

Abstract

Environmental degradation in the Bedadung Watershed (DAS) in Jember has led to natural disasters such as floods and landslides. A conservation effort through a program of planting 1,000 trees in the upstream area of the watershed was initiated to restore environmental health and improve the welfare of the local community. This program was conducted over nine months in Sucopangepok Village, coordinating with local authorities and residents. The tree seedlings, including Teak, Coffee, Sengon, and Soursop, were provided by BPDASHL Brantas Sampean Jember. Planting was done in vacant land using technical, social, and focus group discussion (FGD) approaches. Active community participation was evident in the planting and maintenance of the trees. The outreach and discussions increased awareness about the importance of conservation and disaster mitigation. Planting economically valuable trees is expected to provide long-term benefits for the environment and the community’s economy. The program results showed a reduction in natural disaster risks and an increase in community awareness and participation. Support from the government, private sector, and non-governmental organizations is crucial to ensure the success and sustainability of this conservation program.
PEMBERDAYAAN INDUSTRI KECIL MENENGAH MELALUI AKSI NYATA DESA BERDAYA (PELATIHAN DIGITAL MARKETING DAN PERIJINAN BERUSAHA PADA PELAKU INDUSTRI MAKANAN DI DESA PELEYAN) Fandiyanto, Randika; Irawan, Bambang; Masnilah, Rachmi; Yulisetiarini, Diah; Sudarsih, Sudarsih; Prasetyaningtiyas, Susanti
MIMBAR INTEGRITAS : Jurnal Pengabdian Vol 4 No 2 (2025): AGUSTUS 2025
Publisher : Biro Administrasi dan Akademik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/mimbarintegritas.v4i2.6543

Abstract

This activity was carried out for food industry players in Peleyan Village, Kapongan District, Situbondo Regency. The implementation of the training activity was in collaboration with the Peleyan Village Government, Rumah BUMN, Situbondo, Abdurachman Saleh University, Situbondo and Jember University. The training activity was held on Saturday, May 17, 2025 at the Peleyan village hall. Attended by 30 participants, housewives who are food and beverage business actors in the village area. The purpose of this activity is to provide education about the importance of food business permits such as Business Identification Number (NIB), Household Industry Products (PIRT) and Halal Certification as well as how to make promotional videos. The participants were also given an understanding of the material and practicum in promoting their products through social media Instagram, Tiktok through e-commerce media such as Shopee and Tokopedia. The participants were very enthusiastic in participating in the training activities, marked by several video results of their products that began to be uploaded on each participant's social media. The results of this activity are expected to be able to improve the progress of the small and medium industry sector, especially in the food business in Peleyan Village, Kapongan District, so that they can utilize their legality and digital marketing capabilities in improving their business performance.
PELATIHAN PETANI DALAM MEMPRODUKSI JAMUR TRICHODERMA SP UNTUK MENGELOLA PENYAKIT LAYU PADA TANAMAN CABAI Subekti, Sri; Puspaningrum, Diah; Sunartomo, Aryo Fajar; Mustapit, Mustapit; Nurcahyanti, Suhartiningsih Dwi; Masnilah, Rachmi
MIMBAR INTEGRITAS : Jurnal Pengabdian Vol 4 No 2 (2025): AGUSTUS 2025
Publisher : Biro Administrasi dan Akademik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/mimbarintegritas.v4i2.6522

Abstract

Penyakit layu pada tanaman Cabai merupakan menyakit yang mematikan tanaman. Penyakit layu masih sulit diatasi oleh petani. Kegiatan pengabdian kepada masyarakat bertujuan untuk mengajari petani memproduksi jamur Trichoderma sp yang menjadi musuh alami bagi penyalit layu Fusarium. Kegiatan dilaksanakan di desa Sukowiryo, Kecamatan Jelbuk, Kabupaten Jember. Pelatihan dikhususkan kepada petani yang tergabung dalam kelompok tani Hidayah Tani. Peserta kegiatan adalah petani laki-laki dan perempuan. Hasil pelaksanaan menunjukkan bahwa petani semangat mengikuti kegiatan pelatihan. Trichoderma sp merupakan inovasi bagi petani karena petani belum mengenal Trichoderma sp sebagai musuh alami bagi jamur lain. Kegiatan pelatihan mampu meningkatkan pengetahuan dan keterampilan petani dalam mengelola penyakit layu pada tanaman cabai.
Laboratory Evaluation of the Effectiveness of Paraffin Oil and Insecticide Combination Based on Spinetoram and Permethrin to Control Weaver Ant (Oecophylla smaragdina Fabricius) Habriantono, Bakhroini; Masnilah, Rachmi; Kustiawan Alfarisy, Fariz
Jurnal Ilmiah Inovasi Vol 25 No 1 (2025): April
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Paraffin oil is known to be effective as a pest control agent. It is more toxic to pest insects while being safer for plants, humans, and beneficial organisms that act as natural enemies. Paraffin oil is also known for its ability to be mixed with various other pesticides, which can extend its effectiveness. This capability is particularly useful for reducing insecticide dosages in the field. This study aims to determine the best treatment combination of paraffin oil with insecticides containing the active ingredients spinetoram and permethrin on weaver ants (Oecophylla smaragdina), which in certain cases can become pests in laboratory settings. The findings of this study can potentially be used to minimize insecticide usage. The application of paraffin oil and its combinations with spinetoram and permethrin insecticides was carried out using a topical or direct spraying method on weaver ants. The study was designed using a Completely Randomized Design (CRD) with one factor, varying combinations of paraffin oil with spinetoram and permethrin insecticides. The observed variable was the mortality of weaver ants. The research results showed that the combination of 0.4 ml of paraffin oil with 0.6 ml of permethrin insecticide was the most effective in causing mortality of weaver ant pests and in reducing the dosage of synthetic insecticides.
Screening and Identification of Lactic Acid Bacteria from Livestock Rumen as Probiotic Candidates Widianingrum, Desy Cahya; Tanzil, Ahmad Ilham; Masnilah, Rachmi; Nurcahyanti3, Suhartiningsih Dwi; Fitriani, Vivi; Khasanah, Himmatul
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.104307

Abstract

Population of lactic acid bacteria (LAB) in the rumen of fattening livestock is relatively high and has the potential to be utilized. This study aimed to screen and identify LAB from the rumen as probiotic candidates. The research used rumen liquid from cattle, goats, and sheep collected from slaughterhouses in Bondowoso. The screening and identification processes were conducted at the Livestock Agroindustry Laboratory, Faculty of Agriculture, University of Jember. The screening included colony morphology, Gram staining, cell shape, catalase test, and Carbon dioxide (CO2) gas production ability. The final identification of potential probiotic bacterial candidates was based on their bile salt tolerance. The results showed that there were LAB candidates with 22 isolates (from sheep/OS), 19 isolates (from cattle/OC), and 18 isolates (from goats/OG). The bacterial colony shape included oval (2OS, 1OC), circular (11OS, 18OC, 12OG), irregular (7OS, 6OG), and spindle (2OS). Colony sizes ranged from small (0.5–1.2 mm: 7 OS, 9OC, 4OG), medium (1.2–3.0 mm: 7OS, 7OC, 7OG), to large (3.0–5.0 mm: 8OS, 3OC, 7OG), with the majority of colonies being white and cream-colored, and a minority being yellow. All bacteria were Gram-positive, with coccus (19OS, 14OC, 2OG) and bacillus (3OS, 5OC, 16OG) shaped cells. All samples were capable of producing CO2 gas, and 50% of the isolates possessed catalase enzymes. Based on bile salt tolerance tests, 15% of the isolates had a survival rate of over 50%. In conclusion, LAB from the rumen, particularly goats, were identified as potential probiotic candidates for further development.
The Impact of Biopesticide Application on Arthropod Composition in Surjan Cropping System in Kulonprogo Regency, Special Region of Yogyakarta Wagiyana, Wagiyana; Suharto, Suharto; Habriantono, Bakhroini; Nurcahyanti, Suhartiningsih Dwi; Masnilah, Rachmi; Khozin, Mohammad Nur; Merina, Gusna; Ramadhani, Farchan Mushaf Al; Putra, Dimas Ganda Permana; Alfarisy, Fariz Kustiawan
Journal of Applied Agricultural Science and Technology Vol. 9 No. 3 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i3.298

Abstract

Surjan is a specific type of cropping system that is part of a local wisdom practice found in Kulonprogo Regency, Special Region of Yogyakarta. The cropping system employs a polyculture system with a raised-sunken bed configuration. In ancient times, this system was initiated as a conservation initiative in the event of drought. In the context of agroecosystems, defined as the interaction between biotic and abiotic components, arthropods serve as indicators of the biotic components of the agricultural environment. The presence of arthropods is influenced by the use of synthetic pesticides. In response to the use of synthetic pesticides, biopesticides are frequently used as a countermeasure. The present study aims to ascertain the impact of biopesticide applications on the composition of arthropods in agricultural land that utilizes surjan cropping system configuration. This study was conducted on the surjan cropping system in Kulonprogo Regency, which is predominantly characterized by the cultivation of rice and shallots. The biopesticide used in this study was a group of fungi, namely Trichoderma harzianum and Metarhizium anisopliae. These organisms function as bioprotectants and biofertilizers. The findings indicate that surjan cropping system, when accompanied by biopesticide utilization, yield a greater diversity of arthropod species in comparison to surjan cropping system that employs synthetic pesticides. In agricultural land with surjan system configuration and biopesticide applications, certain arthropods function as predators, parasitoids, and bioindicators. The most prevalent arthropod species identified is Verania sp. (Coleoptera; Coccinelidae), with a total of 68 individuals. The present study has yielded findings indicating a correlation between the application of surjan cropping system and the utilization of biopesticides in land cultivated with a specificcrops and the composition of arthropods in the environment.
Damping-off defense: a comparative analysis of Trichoderma spp. (Hypocreales: Hypocreaceae) isolates against Sclerotium rolfsii (Polyporales: Atheliaceae) in groundnut Chairunnisa, Eka; Masnilah, Rachmi; Nugroho Hadi, Zul Fauzi
Agrovigor Vol 18, No 1 (2025)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v18i1.23934

Abstract

Arachis hypogaea L. (Fabales: Fabaceae), a vital palawija commodity in Indonesia, has seen declining production in Jawa Timur in recent years. Among agricultural challenges, biotic factors like pest and disease infestations significantly impact yield. Damping-off, caused by the fungal pathogen Sclerotium rolfsii, is a major disease impacting groundnut cultivation. Biocontrol agents offer a promising alternative to synthetic fungicides for managing plant pathogens. This study evaluated the effectiveness of various Trichoderma spp. isolates in controlling S. rolfsii damping-off disease in Bison groundnut variety based on four key parameters: incubation period, disease incidence and severity, and control efficacy. The results demonstrated that all tested Trichoderma spp. isolates had the ability to suppress damping-off disease. Notably, isolate TB01 exhibited the best performance significantly prolonging the pathogen's incubation period, reducing disease incidence and severity in both pre- and post-emergence phases, and demonstrating the highest control efficacy 47.95% “sufficiently capable”. This study highlights the potential of Trichoderma spp. as a sustainable and eco-friendly biocontrol agent for managing S. rolfsii damping-off in groundnut cultivation. The successful application of isolate TB01 offers a promising approach for farmers seeking effective disease control strategies with minimal environmental impact.
Co-Authors Abd Latif Abadi Abd Latif Abadi Abdul Majid Abdul Majid Agung Sih Kurnianto Ahmad Ilham Tanzil Ahmad Ilham Tanzil Alfarisy, Fariz Kustiawan Ali Wafa Ankardiansyah Pandu Pradana Aryo Fajar Sunartomo Bakhroini Habriantono Bambang Irawan Bela Indri Ayunita Chairunnisa, Eka Deviana Fitria Astuti Diah Yulisetiarini Dimas Ganda Permana Putra Efrian Kuncoro Fa'ayunina, Miftakhul Fachry Abda El Rahman Fandiyanto, Randika Farchan Mushaf Al Ramadhani Fariz Kustiawan Alfarisy Fitria Dwi Damayanti Gusna Merina, Gusna Guswantoro, Dicky Ade Habriantono, Bakhroini Hakim, Tiara Oktavia Putri HARDIAN SUSILO ADDY Hasbi Mubarak Hasjim, Saifuddin Himmatul Khasanah Hoesain, Mohammad Intan Kartika Setyawati Isnaeni, Siti Juli Khozin, Mohammad Nur Kustiawan Alfarisy, Fariz Lestari, Resti Putri Luqman Qurata Aini Maria Azizah Muhamad Aditia Ulhaq Muslimah, Syafira Ayu Mustapit Mustapit N, Suhartiningsih Dwi Nasriyah Hidayatus Sholeha Nugroho Hadi, Zul Fauzi Nurcahyanti3, Suhartiningsih Dwi Nurchayanti, Suhartiningsih Dwi Nurmala, Fenny Nusantara, Alrio Putra Ollyvia Eswinda Santi Prasetyaningtiyas, Susanti Puspaningrum, Diah Ramadhan, Farchan Mushal Al Rana Virga Tesha Syofiana Rismayani Risqianti, Riza Roni Yulianto RR. Ella Evrita Hestiandari Sari, Dwi Tirta Setiadi Jitendhriyawan Suwarno Shavanna Ardhelia Arijaya Sigit PRASTOWO Siti Mai Saroh Sri Subekti SUDARSIH SUDARSIH Suhartiningsih Dwi N Suhartiningsih Dwi Nurcahyanti Suharto Suharto Tarigan, Sri Ita Thia Mahardikha Ra Putri Tutung Hadi Astono Wagiyana Wagiyana, Wagiyana Widianingrum, Desy Cahya Wildan Muhlison, Wildan WIWIEK SRI WAHYUNI