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KARAKTERISASI ENZIM PHYTASE MIKROBA UNTUK MENINGKATKAN KUALITAS BAHAN PAKAN UNGGAS Listya Purnamasari; Miswar Miswar
Journal of Livestock Science and Production Vol 2, No 2 (2018): Journal of Livestock Science and Production
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jalspro.v2i2.825

Abstract

Asam phytat merupakan salah satu faktor antinutrisi yang terdapat dalam pakan ternak yang berasal dari serealia yang dapat menurunkan kecernaan nutrien bahan pakan sehingga mengganggu produktivitas ternak utamanya ternak monogastrik. Penurunan produktivitas disebabkan karena keterbatasan enzim yang mampu menguraikan asam phytat. Tujuan dari penelitian ini adalah mengetahui aktivitas enzim phytase yang berasal dari mikroba yang ditumbuhkan pada media padat. Media padat yang digunakan ada 4 macam yaitu jagung, dedak padi, dedak jagung dan beras merah. Mikroba yang digunakan yaitu bakteri Basillus subtilis. Pengamatan dilakukan pada rentang pH 4-7 dengan masa inkubasi selama 5 hari pada suhu ruang 25OC di atas shaker. Hasil penelitian menunjukkan bahwa aktivitas fitase dari Basillus subtilis memiliki rentang pH yang panjang yaitu 4-7. Aktivitas dari media padat yang digunakan tidak menunjukkan perbedaan yang nyata sehingga semua media dapat digunakan sebagai media pertumbuhan bakteri yang memproduksi enzim phytase. Enzim fitase yang dihasilkan pada media jagung yaitu 0,313 µg/ml media dedak padi yaitu 0,317 µg/ml media dedak jagung yaitu 0,313 µg/ml dan media beras merah yaitu.0,315 µg /ml. Kesimpulan dari penelitian ini yaitu bakteri Basillus subtilis dapat digunakan untuk memproduksi enzim phytase dan mampu menurunkan kandungan asam fitat pada bahan pakan.Kata kunci : Asam fitat, biji-bijian, enzim phytase, fermentasi media padat
PENINGKATAN KEMANDIRIAN PETERNAK DESA KLABANG MELALUI VILLAGE BREEEDING CENTER DAN PENERAPAN TEKNOLOGI PENGOLAHAN PAKAN LOKAL Listya Purnamasari; Melinda Erdya Krismaputri; Himmatul Khasanah; Nur Widodo
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 9, No 2 (2020): November
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v9i2.43725

Abstract

Kegiatan program pengabdian desa binaan ini dilakukan di Desa Klabang, Kecamatan Tegalampel, Kabupaten Bondowoso. Kegiatan yang dilakukan yaitu peningkatan kemandirian peternak desa Klabang melalui Village Breeeding Center dan penerapan teknologi pengolahan pakan ternak asal sumberdaya lokal. Manajemen budidaya yang tepat dan ketersediaan pakan berkualitas secara mandiri diharapkan mampu meningkatkan reproduksi dalam hal bibit ternak dan produktivitas ternak. Mitra dalam kegiatan program pengabdian desa binaan ini yaitu masyarakat Desa Klabang, Kecamatan Tegalampel, Kabupaten Bondowoso. Metode yang digunakan dalam program ini yaitu, penyuluhan dan pelatihan manajemen pemeliharaan ternak serta recording ternak dan pemanfaatan limbah pertanian sebagai pakan ternak, penyuluhan dan pelatihan formulasi pakan dan pembuatan pakan fermentasi, pendampingan, monitoring dan evaluasi. Hasil dari program pengabdian kepada masyarakat ini adalah kelompok tani-ternak Desa Klabang Kabupaten Bondowoso mampu mengolah limbah pertanian menjadi pakan ruminansia kaya nutrisi. Buku panduan dan video tahapan pembuatan pakan dapat digunakan oleh masyarakat dengan baik sehingga kelompok tani-ternak dapat mengolah limbah menjadi pakan secara mandiri. Kelompok tani ternak desa Klabang selaku mitra program pengabdian ini dapat melaksanakan dan menerapkan teknologi yang telah diberikan dan berusaha untuk selalu kontinu dalam pembuatan pakan serta pengembangan produk silase sebagai upaya meningkatkan kesejahteraan dan keberlanjutan program.
Enrichment Pupuk Organik Asal Kotoran Ternak di CV Gumukmas Multi Farm Jember Dwi Erwin Kusbianto; Listya Purnamasari; Himmatul Khasanah
Jurnal Pengabdian Magister Pendidikan IPA Vol 4 No 4 (2021)
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.276 KB) | DOI: 10.29303/jpmpi.v4i4.1062

Abstract

CV Gumukmas Multi Farm as a service partner is one of the entrepreneurs in the sociopreneur-based livestock sector who collaborates with smallholder farmers as partners. The problem faced by partners is the high population of sheep that are kept so that they produce abundant livestock manure but are not used optimally so that they pollute the environment. The solution is structured training and demonstration in the manufacture of nutrient-rich organic fertilizer. Success in the management of manure waste is indicated by the results of compost that resemble soil and do not smell. This service program has improved the skills of farmers in managing livestock manure waste into processed organic fertilizers that can be used in agricultural land/forage for animal feed and improving the environmental conditions of the cages to be cleaner and healthier.
Penguatan Pembibitan Sapi Potong Lokal dan Optimalisasi Pengolahan Limbah Peternakan di Desa Klabang, Bondowoso Listya Purnamasari; Basuki; Vega Kartika Sari; Himmatul Khasanah
Jurnal Pengabdian Magister Pendidikan IPA Vol 5 No 2 (2022): April-Juni
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.069 KB) | DOI: 10.29303/jpmpi.v5i2.1564

Abstract

Manajemen budidaya yang tepat dan ketersediaan pakan berkualitas secara mandiri diharapkan mampu meningkatkan reproduksi dalam hal bibit ternak, produktivitas ternak dengan hasil yang sesuai harapan. Kelompok ternak desa Klabang, Kecamatan Tegalampel, Kabupaten Bondowoso dalam manajemen budidaya memiliki beberapak kendala diantaranya keterbatasan pengetahuan dalam manajemen budidaya ternak yang baik dan pengolahan pupuk organik dari limbah ternak. Tujuan dari kegiatan pengabdian ini yaitu peningkatan kemampuan dan keterampilan peternak dalam mengusahakan ternak sapi potong dan mampu mengolah limbah ternak. Kegiatan pengabdian ini dilakukan melalui sosialisasi, pendampingan, dan percontohan terstruktur dalam melakukan manajemen budidaya ternak dan pengolahan limbah. Hasil kegiatan menujukkan bahwa para peternak berpartisipasi aktif dalam kegiatan dan dapat melanjutkankegiatan budidaya ternak serta mengolah limbah ternak secara mandiri. Manajemen pemeliharaan ternak yang baik akan menghasilkan produktivitas ternak yang meningkat. Kegiatan pendampingan secara keberlanjutan akan dapat meningkatkan kemandirian peternak sebagai penghasil bibit sapi potong lokal yang unggul.
Respon Fisiologis Domba Ekor Tipis dan Domba Garut Dengan Pemberian Pakan Konsentrat dan Limbah Tauge Listya Purnamasari; Syeh Ahmad Muhammad Basalamah; Sri Rahayu; Sri Darwati
Jurnal Ilmu Peternakan Terapan Vol 4 No 1 (2020): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The present study aimed to evaluate some physiological variables of thin tailed sheep and Garutsheep fed by concentrate and mung bean sprout waste diet. The physiological variables includedrectal temperature, respiration rate, and heart rate. Six Javanese thin tailed sheeps (16.27±0.86kg) and six Garut sheeps (10.68±1.82 kg) which aged less a year were selected for 4 treatments byfactorial design 2 x 2. The first factor was diet (concentrate and bean sprout waste) and the secondfactor was kind of sheep (Javanese thin tailed sheep and Garut sheep). The sheep were fedindividually for a maintenance requirement on body weight. Ambient temperature, relativehumidity, and physiological variables were measured every morning before feeding during 8weeks. The result showed that the physiological response of Javanese thin tailed sheep and Garutsheep fed by concentrate and mung bean sprout waste diet showed no interaction (P>0.05). Thephysiological response of sheep was not significantly different but the feed treatments had asignificant effect (P>0.05). Rectal temperature, heart rate, and respiration rate were still classifiedas normal so the mung bean sprout waste diet can be used as an alternative source of protein tosubstitute concentrate.
Efficacy of Scattering Application and Paint-on Application of Insecticide in Controlling Housefly on a Broiler Farm Ramoncito Cenon T. Asuncion; Listya Purnamasari; Joseph P. Olarve; Joseph F. Dela Cruz
Indonesian Journal of Environmental Management and Sustainability Vol. 6 No. 3 (2022): September
Publisher : Research Centre of Inorganic Materials and Complexs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/ijems.2022.6.3.82-87

Abstract

The study was performed to determine the efficacy of dinotefuran in controlling housefly (Musca domestica) infestation on a commercial broiler farm. The efficacy of the two modes of application of the insecticide was compared to thiamethoxam. The experiment was divided into two groups with five treatments (T) and three replicates per treatment. The first group was scatter application composed of T1: control, T2: dry 20 g dinotefuran, T3: wet 20 g dinotefuran, T4: dry 20 g thiamethoxam, and T5: wet 20 g thiamethoxam. The second group was a paint-on application composed of T1: control, T2: 10 g dinotefuran with 10 g sugar, T3: 20 g dinotefuran, T4: 10 g thiamethoxam with 10 g sugar, and T5: 20 g thiamethoxam. Dead flies were counted after 15, 30, 60, and 120 minutes post-application. In the scatter group, the control was significantly different from dinotefuran and thiamethoxam, but no significant differences were observed between dinotefuran and thiamethoxam. Dinotefuran treatments from paint-on were significantly different from the other three treatments. Lastly, wet dinotefuran from the scattering bait group and dinotefuran treatments from paint were not significantly different. Wet and paint-on treatments of dinotefuran were effective for fly control in broiler farms.
Black Soldier Fly (Hermetia illucens) as a Potential Agent of Organic Waste Bioconversion Listya Purnamasari; Himmatul Khasanah
ASEAN Journal on Science and Technology for Development Vol. 39 No. 2 (2022): Green Economy
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29037/ajstd.780

Abstract

The use of black soldier fly (BSF) as a bioconversion agent has become an emerging breakthrough in waste processing. Organic wastes, such as household waste and livestock manure, can be used as a growth medium for BSF larvae and converted into favorable products. The average composting time of BSF larvae is around 12– 15 days, which is faster than that of microbes or earthworms (4–5 weeks). BSF shows potential as a feed and food ingredient because it has a high nutritional content, such as enzyme, chitin, medium-chain fatty acid, and antimicrobial peptides, and can be used as a functional food ingredient. From an economical perspective, the short composting period and the role of BSF as a feed and food alternatives can benefit producers and consumers. The safety aspects of BSF utilization, including microbial safety, chemical safety, and environmental safety, warrant clarification to ensure BSF safety. However, some challenges arise regarding the use of BSF larvae (BSFL) as a bioconversion agent, such as for heavy metal residues, pesticide residues, pathogens, and antimicrobial gene transmission and residues that require the best composting strategy for mitigation. The environmental safety of organic waste treated with BSFL has a good impact; therefore, this strategy can be used to reduce global warming. Research must focus on effectively and safely enhancing the cultivation and processing of BSF and its applications as a functional food. In conclusion, BSF is a profitable alternative for organic waste bioconversion in developed and developing countries.
Comparative Efficacy of Different Fixed Drug Combinations on Clinical Signs of Respiratory Disease in Starter Pigs Candice Mabette Habawel; Listya Purnamasari; Joseph Peñano Olarve; Joseph Flores dela Cruz
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.327 KB) | DOI: 10.19087/jveteriner.2022.23.3.297

Abstract

The high occurrence of respiratory disease in pigs has led to the innovation of fixed-dose drug combinations (FDCs). A study was conducted to determine the efficacy of two FDCs on 15 starter pigs showing clinical signs of respiratory disease to determine its effect on their respiratory health and growth. Treatment 1 (T1) was the control group and did not receive any medication. Treatment 2 (T2) contains 90 g of Doxycycline, 40 g of Tylosin, 30 g of Paracetamol, 5 g of Bromhexine, and 500 mg of Prednisolone as active ingredients per kilogram. Treatment 3 (T3) contains 150 g of Amoxicillin Trihydrate, 100 g of Tylosin Tartrate, and 5 g of Bromhexine Hydrochloride as active ingredients per kilogram. The treatment gives at a therapeutic dose of 10 g/gallon of water twice a day for 7 days.The effects of FDCs were measured through clinical sign evaluation, gross pathologic lung lesion scoring, histopathologic examination, and evaluation of the production performance of the starter pigs using analysis of variance (ANOVA) for a Completely Randomized Design. Pigs treated with Treatment 2 had better clinical evaluation scores and production performance than Treatment 3. Histopathologic examination demonstrated minimal tissue repair in all FDCs studied. Improvement denotes that the treatment produces a positive effect.
PERFORMANCE OF DAIRY COWS DURING DIFFERENT SEASONS WITH DAILY HERBAGE ALLOWANCE: A REVIEW Listya Purnamasari; Josep Dela Cruz
Jambura Journal of Animal Science Vol 5, No 1 (2022): Jambura Journal of Animal Science
Publisher : Animal Husbandry Department, Faculty of Agriculture Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35900/jjas.v5i1.15334

Abstract

Pasture-based feeds are favored nowadays due to their cost-effectiveness and effect on animal welfare. Daily herbage allowance (DHA) affects the performance of dairy cows across different seasons. The key determinant of the performance of livestock is the dry matter intake (DMI) of herbage in pasture-based systems. A decline in average summer rainfall may result in considerably higher concentrate supplementation requirements to compensate for the loss in pasture growth and availability during autumn. Supplementation during autumn and winter boost total DMI and sustain milk output under grazing restrictions. Generally, milk production increases at high DHA but has no significant influence on milk fat content. In Spring, greater milk production lessens cows’ maintenance energy needs, and lower pasture content is linked to lower methane generation in the rumen. Methane generation declines in summer with increased digestibility at high intake levels. It is recommended to conduct an experiment exhibiting all seasons to eliminate factors that may affect the results across various studies. This review will understand variation in daily herbage intake of cows at pasture that allows the management of total intake to optimize milk production and identification of cows that are more efficient at converting feed into milk.
In vivo and In vitro anti-diabetic effects of cinnamon (Cinnamomum sp.) plant extract: A review Alyssa MA Nazareno; Listya Purnamasari; Joseph F dela Cruz
jurnal1 VOLUME 5 ISSUE 2, DECEMBER 2022
Publisher : Hasanuddin University Food Science and Technology Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/canrea.v5i2.673

Abstract

Research regarding the enormous potential of medicinal plants for the development of new drugs and the efficient treatment of diabetes mellitus is increasing due to the harmful effects that synthetic drugs may bring such as severe hypoglycemia, abdominal discomfort, lactic acidosis, and more. Cinnamomum sp. (cinnamon) extracts have shown to have significant anti-diabetic effects on type 2 diabetes mellitus in experimental rodent animals in a dose-dependent manner. There are different possible mechanisms of action involved in its anti-diabetic activities. The efficacy of cinnamon extract as an anti-diabetic agent in type 1 diabetes mellitus experimental rodent animal studies explored in this study.