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All Journal VALENSI Jurnal Kesehatan Lingkungan indonesia AL KAUNIYAH FLYWHEEL : Jurnal Teknik Mesin Untirta Indonesian Journal of Chemistry Jurnal Kartika Kimia Molekul: Jurnal Ilmiah Kimia Jurnal Riset Teknologi Industri ASEAN Journal of Community Engagement Journal of Community Service Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Bulletin of Chemical Reaction Engineering & Catalysis LIMNOTEK Jurnal Sains Materi Indonesia Eksplorium : Buletin Pusat Pengembangan Bahan Galian Nuklir
Agustino Zulys
Departement Of Chemistry, Faculty Of Mathematics And Natural Science, Universitas Indonesia, Depok, West Java 1624, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Public Health Social Sciences Transportation Other

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Journal : limnotek

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Quantitative Prediction of Spirulina platensis Biomass Using UV-Vis Spectrophotometry Fajar Sumi Lestari; Agustino Zulys; Awalina Satya
LIMNOTEK Perairan Darat Tropis di Indonesia Vol. 32 No. 1 (2026)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/limnotek.2026.14459

Abstract

Spirulina platensis has become a promising feedstock for the synthesis of several industrially important biomolecules, including proteins, lipids, and carotenoids. However, significant technological obstacles pertaining to optimization, growth monitoring, and biomolecule extraction in Spirulina remain despite advancements in industrial-scale microalgae production and biomolecule harvesting. Standard techniques used for microalgal biomass and biomolecules monitoring include FTIR spectroscopy, colorimetric techniques, and manual cell counting. However, these techniques have drawbacks, particularly processing time and handling errors. This paper seeks to establish an operational equation that effectively relates measured absorbance (or optical density, OD) to the dry weight of Spirulina platensis microalgae using a UV-Vis spectrophotometer. The wavelengths of 680, 750, and 565 nm were selected based on the absorption spectrum of chlorophyll-a, as well as the wavelengths at which absorbance does not reach its peak. The best results were obtained at a wavelength of 680 nm with the equation y = 1.2759x + 0.1512, with an R² value of 0.9914. This technique allows for more accurate measurement of Spirulina platensis dry weight and total biomass.
Co-Authors Adawiah Adawiah Adawiah Adawiah Adawiah Adawiah Adawiah Adawiah Adawiah Adawiah Adawiah Adawiah Adawiah Adawiah adawiah adawiah, adawiah Ade Mulyawan Adriana, Elva Dissa Aisyah Safrina Amelia Rosvita Andriyani, Leni Ary Maulana Asep Mohamad Noor Asep Saefumillah Awalina Satya Bale, Uly Fitria Bambang Heru Susanto, Bambang Heru Chalid, Mochamad Citra Santikasari Dede Sukandar Defania, Mella Didin Sahidin Winatapura Dini Rahma Bintari Ely Setiawan Fadhilla, Rizka Fadhilla, Rizkha Fajar Sumi Lestari Farhah Syahidatul Mala Farhan Maulana Azhar Ginting, Grace Gardenia Hadriyani, Rizka Haidir, Banu Muhammad Harahap, Lina Yuliana Hayati Sari Hasibuan Iman Abdullah Isalmi Aziz Istiqomah, Muhsinatul Iwan Susanto Jan Setiawan Jarnuzi Gunlazuardi Kurnia Trinopiawan Latifah Tulhusna Lina Yuliana Harahap Mabuchi, Takuya Mai Saroh Ambar Pramukti Masripah Masripah Meyliana Wulandari Muhammad Derry Luthfi Yudhi Muhammad Derry Luthfi Yudhi Muhammad Fathurrahman Muhammad Ghozali Muhammad Shofyan Gunawan Nanda Saridewi Nanda Saridewi Nanda Saridewi, Nanda Nasruddin Nasruddin Nasruddin Nova Anita Nur Mahrunnisa Nurhasni Nurhasni Permana, Sulaksana Pramukti, Mai Saroh Ambar Ramadhan, Fitri Nur Ridla Bakri Riesna Prassanti Rika Tri Yunarti Risma Nur Fitria Salman Farishi Sari Hasnah Dewi Selviana Rustanti Siti Mutia Mawaddah Siti Nurbayti Siti Nurbayti Siti Nurbayti Siti Nurbayti Soedarsono, Johny W. Sri Komala Sri Komala Suminta, Tiara Amalia Syauqi, Muhammad Iqbal Takhwifa, Famila Tulhusna, Latifah Wahyudin Wahyudin Widiantari Nofriandani Wisnu Ari Adi Wulandari Oktavia Yani, Sela Vivi