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All Journal HAYATI Journal of Biosciences eDimensi Arsitektur Petra Jurnal EMT KITA ANNALES BOGORIENSES Indonesian Journal of Biotechnology and Biodiversity Jurnal Sains dan Teknologi Farmasi Indonesia Journal of Innovation in Teaching and Instructional Media Journal of Social Work and Science Education E-Amal: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmiah Pariwisata Annales Bogorienses
DESSY NATALIA
Program Studi Teknik Kimia, FTI, Universitas Katolik Parahyangan, Jl. Ciumbuleuit No 94, Bandung
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Other

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Construction of an EPO (Human-Erythropoietin) Synthetic Gene Through a Recurvise-PCR Method Fuad, Asrul Muhamad; Gusdinar, Tutus; Retnoningrum, Debbie Sofie; Natalia, Dessy
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : BRIN

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Abstract

Human erythropoietin (hEPO) is an important glycoprotein in human that is coded by a single gene named EPO (erythropoietin). EPO is a glycoprotein hormone that promotes erythropoiesis, which is the formation process of mature red blood cell (erythr eyte ) in human bodies. It is widely used for treatment of anemia in patient with chronic renal failure. Therefore EPO has been classified as hematopoietic cytokine. Recombinant hEPO (rhEPO) has been commercially available, such a Epogen. It is produced in mammalian cell, such as CHO (Chinese hamster ovary) ceil for the reason of it· complex structure as a glyco-protein. In an effort to use and optimize heterologous EPO gene expression in an alternative eukaryotic host cells such as yeast, an EPO synthetic gene (EPOsyn) was constructed. The synthetic gene had been designed to contain optimal Pichia pastoris codon usage. It had been constructed by a recursive-PCR method in two-step PCR reactions. The gene was assembled from 8 single strands synthetic oligonucleotides having an average length of 90 nt with 20 to 30 overlap region between two adjacent oligos. The synthetic gene has less GC content (45.31%) compared its native (human) gene (59.08%). The synthetic gene has been cloned in pCR2.1 cloning plasmid and sequenced. From 8 independent clones, it was revealed that the error rate was 1.59%, in which 1.42% was due to deletions and 0.17% due to substitutions. Design of the gene sequences, construction method and DNA sequence analysis of the gene will be discussed in this paper.
Pariwisata Berkelanjutan Sebagai Upaya Penguatan Destinasi Wisata Pasca Pandemi di Desa Ekowisata Pancoh Sleman Yogyakarta Sri Puspa Dewi, Roels Ni Made; Natalia, Dessy; Lorenza, Felicia Augustine
Jurnal Ilmiah Pariwisata Vol 28 No 2 (2023): Jurnal Ilmiah Pariwisata
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat, Institut Pariwisata Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30647/jip.v28i2.1695

Abstract

The purpose of this study is to identify ecotourism activities that lead to the concept of sustainable tourism. Furthermore, from this description, one can examine the sustainability of tourism from three aspects, namely environmental, economic, and socio-cultural. The research method used is qualitative research with an exploratory approach. Data collection methods through observation, interviews, and documentation. Retrieval of informants with purposive sampling which took as many as 20 people as sources. The data that has been collected is analyzed with an interactive model. The results of this study are that the Pancoh Ecotourism Village has implemented the principles of sustainable tourism starting from participation, carrying capacity and promotion. The implementation of sustainable tourism from the socio-cultural aspect is in the form of incorporating elements of local culture into tourist attractions such as the Wiwitan tradition and learning gamelan. Environmental aspects by utilizing sustainable resources such as recycling waste, using cow dung to become biogas, and making crafts from local bamboo materials. The economic aspect is increasing people's welfare through empowering homestays. The benefits obtained from these three aspects have made Pancoh Ecotourism Village maintain its existence and strengthen destinations through tourism activities based on conservation and utilizing existing resources. Keywords: Sustainable Tourism,Destination Strengthening, Post Pandemic, Ecotourism
The Impact of Attractiveness, Facilities, and Service Quality on Visitor Satisfaction: A Case Study of XYZ Attraction Kalimin, Evan Crysthiansen; brian, Reagan; Natalia, Dessy
Jurnal EMT KITA Vol 10 No 3 (2026): JULY 2026
Publisher : Lembaga Otonom Lembaga Informasi dan Riset Indonesia (KITA INFO dan RISET) - Lembaga KITA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35870/emt.v10i3.6313

Abstract

Visitor satisfaction is a crucial indicator in maintaining the sustainability and competitiveness of a destination amidst the intense competition within the oceanarium industry in the Greater Jakarta area. The urgency of this study is driven by 2025 review data, which indicates that a leading marine educational tourism destination in Jakarta holds the lowest rating compared to its main competitors, suggesting a significant gap in visitor satisfaction. The primary issues identified include a decline in visual appeal due to poor aquarium cleanliness, poorly maintained facilities, and low service quality resulting from the presence of unauthorized third parties and less responsive staff. This study aims to empirically analyze the influence of attraction, facilities, and service quality on visitor satisfaction at the destination. Utilizing a quantitative method with a survey approach involving 173 respondents, the data were analyzed using the PLS-SEM technique via the SmartPLS 4.0 application. The results demonstrate that all three independent variables have a positive and significant effect on visitor satisfaction. The facility variable was identified as the most dominant factor in shaping satisfaction with a path coefficient of 0.494, followed by service quality (0.312) and attraction (0.215). Overall, this research model explains 93.4% of the fluctuations in visitor satisfaction. These findings provide practical contributions for the management to prioritize facility revitalization, enhance service standards through staff training, and improve aquarium cleanliness to create a more memorable tourism experience and increase visitor loyalty.
Co-Authors . Anindyajati Afifah, Salma Aulia Antonius Rizki Krisnadi ARTARINI, ANITA Asrul Muhamad Fuad, Asrul Muhamad Brian, Reagan CATUR RIANI David Andrio DEBBIE SOFIE RETNONINGRUM Dewantara, Yudhiet Fajar Dewi Astriany Djunaid, Ika Suryono Djunaidi Djunaidi Effendi, Syulastri Enny Ratnaningsih Ernawati A. Giri Rachman Facrureza, Dewanta Febriani Aji Kusuma, Sherlynda Hema, Lamtiar Imam Ardiansyah Iman Permana Maksum Iskandar, Hari Kalimin, Evan Crysthiansen Kusumawardhani, Shinta Lorenza, Felicia Augustine Marisa Handajani Mindriany Syafila Nora, Adri Nur Ahyani Roels Ni Made Sri Puspa Dewi, Roels Ni Made Rommy Qurniati Rosanto, Stephanie Slamet Budi Yuwono Susanto, Prayogo Tan, Marselina Irasonia Toto Subroto TUTUS GUSDINAR Vishnuvardhana, Vishnuvardhana