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All Journal HAYATI Journal of Biosciences eDimensi Arsitektur Petra ANNALES BOGORIENSES Indonesian Journal of Biotechnology and Biodiversity Acta Biochimica Indonesiana Jurnal Kewarganegaraan Jurnal Sains dan Teknologi Farmasi Indonesia Jurnal TNI Angkatan Udara Journal of Innovation in Teaching and Instructional Media Journal of Social Work and Science Education E-Amal: Jurnal Pengabdian Kepada Masyarakat Annales Bogorienses
DESSY NATALIA
Program Studi Teknik Kimia, FTI, Universitas Katolik Parahyangan, Jl. Ciumbuleuit No 94, Bandung
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Expression of Recombinant Plasmodium falciparum Cysteine-rich Protective Antigen (PfCyRPA) Fragment 26–181 in Escherichia coli BL21 CodonPlus (DE3) RIPL Masduki, Fifi Fitriyah; Prameswari, Aurelia Galih; Puspasari, Fernita; Ihsanawati, Ihsanawati; Natalia, Dessy
Acta Biochimica Indonesiana Vol. 8 No. 1 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.217

Abstract

Background: Malaria is a life-threatening infectious disease caused by Plasmodium parasites, transmitted through infected female Anopheles mosquitoes. PfCyRPA (Plasmodium falciparum Cysteine-Rich Protective Antigen) has emerged as a promising vaccine candidate due to its ability to elicit inhibitory antibodies against parasite growth. Objective: This study aimed to construct recombinant plasmids encoding PfCyRPA fragment 26–181 and express this fragment in Escherichia coli BL21 CodonPlus (DE3) RIPL for cost-effective antigen production. Methods: The PfCyRPA gene fragment (~480 bp) was amplified from P. falciparum genomic DNA (Jayapura isolate) by PCR. Recombinant plasmids pGEM-T-PfCyRPA 26-181 and pET-16b-PfCyRPA 26-181 were constructed and confirmed via colony PCR, restriction analysis, and sequencing. The pET-16b-PfCyRPA 26-181 was transformed into E. coli BL21 CodonPlus (DE3) RIPL. Protein expression was induced with 0.5 mM IPTG at 37°C, and analyzed by SDS-PAGE and Western blotting. Results: SDS-PAGE and Western blot analysis demonstrated successful expression of recombinant PfCyRPA fragment 26–181 with a molecular mass of approximately 21.53 kDa, corresponding to the predicted size. The protein was predominantly expressed as inclusion bodies, typical for eukaryotic proteins in prokaryotic systems. Colony PCR and sequencing confirmed correct gene insertion and integrity. Conclusion: Recombinant PfCyRPA fragment 26–181 was successfully expressed in E. coli BL21 CodonPlus (DE3) RIPL, providing a cost-effective platform for large-scale antigen production. This work establishes a foundational protocol for further immunogenicity research and supports development of this antigen as a potential blood-stage malaria vaccine candidate.
The Impact of Incentives and Work Discipline on Teacher Performance in Tanjung Lago Junior High Schools Natalia, Dessy; Ahyani, Nur; Djunaidi, Djunaidi
Journal of Social Work and Science Education Vol. 6 No. 2 (2025): Journal of Social Work and Science Education
Publisher : Yayasan Sembilan Pemuda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52690/jswse.v6i2.1204

Abstract

This quantitative study examines the effects of incentives and work discipline on teacher performance in Tanjung Lago District, Banyuasin. This study was conducted at SMP Negeri Sekecamatan Tanjung Lago Kabupaten Banyuasin. In this study, using quantitative research methods. The methods used are correlation test methods and partial and simultaneous tests. Using a Likert-scale questionnaire administered to 82 teachers, data were analyzed via SPSS 26. Results indicate that incentives (45.3%) and work discipline (47.4%) significantly enhance teacher performance. The findings underscore the importance of fair incentive systems and strict work discipline in improving educational outcomes. There is a significant effect of incentives and work discipline on the performance of junior high school teachers in Tanjung Lago sub-district. The conclusion is that there is a significant effect of incentives and work discipline on the performance of teachers in Tanjung Lago sub-district.
Analisis Lingkungan Strategis Wilayah Perbatasan: Studi Kasus Ambalat Agastya A. S. W, Rejang Musi; Natalia, Dessy; Widodo, Pujo; Laksmono, Rudy
Jurnal Kewarganegaraan Vol 6 No 2 (2022): Desember 2022
Publisher : UNIVERSITAS PGRI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31316/jk.v6i2.3170

Abstract

AbstrakBlok Ambalat memiliki luas 15.235 km² dan terletak di Kalimantan Utara, tepat di perbatasan Indonesia-Malaysia. Sengketa Ambalat semakin menjadi rumit ketika blok ini diidentifikasi kaya akan potensi sumber daya alam berupa minyak dan gas bumi. Saat ini area Ambalat terdapat blok East Ambalat yang dioperasikan oleh Pertamina Hulu Energi (PHE) East Ambalat. Namun, batas blok East Ambalat yang ditentukan oleh Pemerintah Indonesia tersebut mengalami tumpeng tindih dengan batas blok Shell milik Malaysia. Permasalahan tersebut membawa isu pertahanan menjadi sektor yang perlu ditegakkan untuk kedua negara saling mempertahankan haknya. Hingga saat ini, persoalan Ambalat masih belum menghasilkan penyelesaian akhir antara sengketa dua negara. Penyelasaian sengketa yang cukup lama ini memberikan dampak tersendiri bagi negara Indonesia, mulai dari otoritas secara politik, ekonomi, dan sosial budaya. Artikel ini menggunakan metode penelitian kualitatif yang mencoba mendeskripsikan masalah menggunakan studi literatur.Kata Kunci: Perbatasan, Ambalat AbstractThe Ambalat block has an area of 15,235 km² and is located in North Kalimantan, right on the Indonesia-Malaysia border. The Ambalat dispute became even more complicated when the block was identified as rich in potential natural resources in the form of oil and gas. Currently, the Ambalat area has the East Ambalat block operated by Pertamina Hulu Energi (PHE) East Ambalat. However, the East Ambalat block boundary set by the Indonesian Government overlapped with malaysia's Shell block boundary. This problem brings the issue of defense into a sector that needs to be enforced for both countries to defend each other's rights. Until now, the Ambalat issue has not yet produced a final settlement between the two countries' dispute. The long-standing dispute dispute has its own impact on the Indonesian state, starting from the authority politically, economically, and socio-culturally. This article uses qualitative research methods that try to describe the problem using literature studies.Keywords: Border, Ambalat
Construction of an EPO (Human-Erythropoietin) Synthetic Gene Through a Recurvise-PCR Method Fuad, Asrul Muhamad; Gusdinar, Tutus; Retnoningrum, Debbie Sofie; Natalia, Dessy
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Human erythropoietin (hEPO) is an important glycoprotein in human that is coded by a single gene named EPO (erythropoietin). EPO is a glycoprotein hormone that promotes erythropoiesis, which is the formation process of mature red blood cell (erythr eyte ) in human bodies. It is widely used for treatment of anemia in patient with chronic renal failure. Therefore EPO has been classified as hematopoietic cytokine. Recombinant hEPO (rhEPO) has been commercially available, such a Epogen. It is produced in mammalian cell, such as CHO (Chinese hamster ovary) ceil for the reason of it· complex structure as a glyco-protein. In an effort to use and optimize heterologous EPO gene expression in an alternative eukaryotic host cells such as yeast, an EPO synthetic gene (EPOsyn) was constructed. The synthetic gene had been designed to contain optimal Pichia pastoris codon usage. It had been constructed by a recursive-PCR method in two-step PCR reactions. The gene was assembled from 8 single strands synthetic oligonucleotides having an average length of 90 nt with 20 to 30 overlap region between two adjacent oligos. The synthetic gene has less GC content (45.31%) compared its native (human) gene (59.08%). The synthetic gene has been cloned in pCR2.1 cloning plasmid and sequenced. From 8 independent clones, it was revealed that the error rate was 1.59%, in which 1.42% was due to deletions and 0.17% due to substitutions. Design of the gene sequences, construction method and DNA sequence analysis of the gene will be discussed in this paper.
Co-Authors Afifah, Salma Aulia Agastya A. S. W, Rejang Musi Anindyajati Antonius Rizki Krisnadi ARTARINI, ANITA Asrul Muhamad Fuad, Asrul Muhamad CATUR RIANI David Andrio DEBBIE SOFIE RETNONINGRUM Dewantara, Yudhiet Fajar Dewi Astriany Djunaid, Ika Suryono Djunaidi Djunaidi Effendi, Syulastri ENNY RATNANINGSIH ERNAWATI ARIFIN GIRI-RACHMAN Facrureza, Dewanta Fahmi, Ice Febriani Aji Kusuma, Sherlynda Hema, Lamtiar IHSANAWATI IHSANAWATI Imam Ardiansyah Iman Permana Maksum Iskandar, Hari Kusumawardhani, Shinta Laksmono, Rudy Marisa Handajani Masduki, Fifi Fitriyah Maulani, Muhammad Mindriany Syafila Nora, Adri Nugroho Sasongko Jati, Nugroho Sasongko Nur Ahyani Prameswari, Aurelia Galih Pujo Widodo, Pujo Puspasari, Fernita Rommy Qurniati Rosanto, Stephanie Slamet Budi Yuwono Soelistyo, Tri Susanto, Prayogo Tan, Marselina Irasonia Toto Subroto TUTUS GUSDINAR Vishnuvardhana, Vishnuvardhana Yoesgiantoro, Donny