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All Journal HAYATI Journal of Biosciences eDimensi Arsitektur Petra ANNALES BOGORIENSES Indonesian Journal of Biotechnology and Biodiversity Acta Biochimica Indonesiana Jurnal Sains dan Teknologi Farmasi Indonesia Jurnal TNI Angkatan Udara Journal of Innovation in Teaching and Instructional Media Journal of Social Work and Science Education E-Amal: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmiah Pariwisata Annales Bogorienses
DESSY NATALIA
Program Studi Teknik Kimia, FTI, Universitas Katolik Parahyangan, Jl. Ciumbuleuit No 94, Bandung
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Other

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Expression of Recombinant Plasmodium falciparum Cysteine-rich Protective Antigen (PfCyRPA) Fragment 26–181 in Escherichia coli BL21 CodonPlus (DE3) RIPL Masduki, Fifi Fitriyah; Prameswari, Aurelia Galih; Puspasari, Fernita; Ihsanawati, Ihsanawati; Natalia, Dessy
Acta Biochimica Indonesiana Vol. 8 No. 1 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.217

Abstract

Background: Malaria is a life-threatening infectious disease caused by Plasmodium parasites, transmitted through infected female Anopheles mosquitoes. PfCyRPA (Plasmodium falciparum Cysteine-Rich Protective Antigen) has emerged as a promising vaccine candidate due to its ability to elicit inhibitory antibodies against parasite growth. Objective: This study aimed to construct recombinant plasmids encoding PfCyRPA fragment 26–181 and express this fragment in Escherichia coli BL21 CodonPlus (DE3) RIPL for cost-effective antigen production. Methods: The PfCyRPA gene fragment (~480 bp) was amplified from P. falciparum genomic DNA (Jayapura isolate) by PCR. Recombinant plasmids pGEM-T-PfCyRPA 26-181 and pET-16b-PfCyRPA 26-181 were constructed and confirmed via colony PCR, restriction analysis, and sequencing. The pET-16b-PfCyRPA 26-181 was transformed into E. coli BL21 CodonPlus (DE3) RIPL. Protein expression was induced with 0.5 mM IPTG at 37°C, and analyzed by SDS-PAGE and Western blotting. Results: SDS-PAGE and Western blot analysis demonstrated successful expression of recombinant PfCyRPA fragment 26–181 with a molecular mass of approximately 21.53 kDa, corresponding to the predicted size. The protein was predominantly expressed as inclusion bodies, typical for eukaryotic proteins in prokaryotic systems. Colony PCR and sequencing confirmed correct gene insertion and integrity. Conclusion: Recombinant PfCyRPA fragment 26–181 was successfully expressed in E. coli BL21 CodonPlus (DE3) RIPL, providing a cost-effective platform for large-scale antigen production. This work establishes a foundational protocol for further immunogenicity research and supports development of this antigen as a potential blood-stage malaria vaccine candidate.
The Impact of Incentives and Work Discipline on Teacher Performance in Tanjung Lago Junior High Schools Natalia, Dessy; Ahyani, Nur; Djunaidi, Djunaidi
Journal of Social Work and Science Education Vol. 6 No. 2 (2025): Journal of Social Work and Science Education
Publisher : Yayasan Sembilan Pemuda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52690/jswse.v6i2.1204

Abstract

This quantitative study examines the effects of incentives and work discipline on teacher performance in Tanjung Lago District, Banyuasin. This study was conducted at SMP Negeri Sekecamatan Tanjung Lago Kabupaten Banyuasin. In this study, using quantitative research methods. The methods used are correlation test methods and partial and simultaneous tests. Using a Likert-scale questionnaire administered to 82 teachers, data were analyzed via SPSS 26. Results indicate that incentives (45.3%) and work discipline (47.4%) significantly enhance teacher performance. The findings underscore the importance of fair incentive systems and strict work discipline in improving educational outcomes. There is a significant effect of incentives and work discipline on the performance of junior high school teachers in Tanjung Lago sub-district. The conclusion is that there is a significant effect of incentives and work discipline on the performance of teachers in Tanjung Lago sub-district.
Construction of an EPO (Human-Erythropoietin) Synthetic Gene Through a Recurvise-PCR Method Fuad, Asrul Muhamad; Gusdinar, Tutus; Retnoningrum, Debbie Sofie; Natalia, Dessy
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : BRIN

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Abstract

Human erythropoietin (hEPO) is an important glycoprotein in human that is coded by a single gene named EPO (erythropoietin). EPO is a glycoprotein hormone that promotes erythropoiesis, which is the formation process of mature red blood cell (erythr eyte ) in human bodies. It is widely used for treatment of anemia in patient with chronic renal failure. Therefore EPO has been classified as hematopoietic cytokine. Recombinant hEPO (rhEPO) has been commercially available, such a Epogen. It is produced in mammalian cell, such as CHO (Chinese hamster ovary) ceil for the reason of it· complex structure as a glyco-protein. In an effort to use and optimize heterologous EPO gene expression in an alternative eukaryotic host cells such as yeast, an EPO synthetic gene (EPOsyn) was constructed. The synthetic gene had been designed to contain optimal Pichia pastoris codon usage. It had been constructed by a recursive-PCR method in two-step PCR reactions. The gene was assembled from 8 single strands synthetic oligonucleotides having an average length of 90 nt with 20 to 30 overlap region between two adjacent oligos. The synthetic gene has less GC content (45.31%) compared its native (human) gene (59.08%). The synthetic gene has been cloned in pCR2.1 cloning plasmid and sequenced. From 8 independent clones, it was revealed that the error rate was 1.59%, in which 1.42% was due to deletions and 0.17% due to substitutions. Design of the gene sequences, construction method and DNA sequence analysis of the gene will be discussed in this paper.
Pariwisata Berkelanjutan Sebagai Upaya Penguatan Destinasi Wisata Pasca Pandemi di Desa Ekowisata Pancoh Sleman Yogyakarta Sri Puspa Dewi, Roels Ni Made; Natalia, Dessy; Lorenza, Felicia Augustine
Jurnal Ilmiah Pariwisata Vol 28 No 2 (2023): Jurnal Ilmiah Pariwisata
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat, Institut Pariwisata Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30647/jip.v28i2.1695

Abstract

The purpose of this study is to identify ecotourism activities that lead to the concept of sustainable tourism. Furthermore, from this description, one can examine the sustainability of tourism from three aspects, namely environmental, economic, and socio-cultural. The research method used is qualitative research with an exploratory approach. Data collection methods through observation, interviews, and documentation. Retrieval of informants with purposive sampling which took as many as 20 people as sources. The data that has been collected is analyzed with an interactive model. The results of this study are that the Pancoh Ecotourism Village has implemented the principles of sustainable tourism starting from participation, carrying capacity and promotion. The implementation of sustainable tourism from the socio-cultural aspect is in the form of incorporating elements of local culture into tourist attractions such as the Wiwitan tradition and learning gamelan. Environmental aspects by utilizing sustainable resources such as recycling waste, using cow dung to become biogas, and making crafts from local bamboo materials. The economic aspect is increasing people's welfare through empowering homestays. The benefits obtained from these three aspects have made Pancoh Ecotourism Village maintain its existence and strengthen destinations through tourism activities based on conservation and utilizing existing resources. Keywords: Sustainable Tourism,Destination Strengthening, Post Pandemic, Ecotourism
Co-Authors Afifah, Salma Aulia Agastya A. S. W, Rejang Musi Anindyajati Antonius Rizki Krisnadi ARTARINI, ANITA Asrul Muhamad Fuad, Asrul Muhamad CATUR RIANI David Andrio DEBBIE SOFIE RETNONINGRUM Dewantara, Yudhiet Fajar Dewi Astriany Djunaid, Ika Suryono Djunaidi Djunaidi Effendi, Syulastri ENNY RATNANINGSIH ERNAWATI ARIFIN GIRI-RACHMAN Facrureza, Dewanta Fahmi, Ice Febriani Aji Kusuma, Sherlynda Hema, Lamtiar IHSANAWATI IHSANAWATI Imam Ardiansyah Iman Permana Maksum Iskandar, Hari Kusumawardhani, Shinta Laksmono, Rudy Lorenza, Felicia Augustine Marisa Handajani Masduki, Fifi Fitriyah Maulani, Muhammad Mindriany Syafila Nora, Adri Nugroho Sasongko Jati, Nugroho Sasongko Nur Ahyani Prameswari, Aurelia Galih Pujo Widodo, Pujo Puspasari, Fernita Roels Ni Made Sri Puspa Dewi, Roels Ni Made Rommy Qurniati Rosanto, Stephanie Slamet Budi Yuwono Soelistyo, Tri Susanto, Prayogo Tan, Marselina Irasonia Toto Subroto TUTUS GUSDINAR Vishnuvardhana, Vishnuvardhana Yoesgiantoro, Donny