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Asmuddin Natsir
Faculty Of Animal Husbandy, Hasanuddin University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Purine derivatives excretion and estimation of microbial protein supply on sheep receiving different protein suplements Asmuddin Natsir
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 3 (2007): SEPTEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.38 KB) | DOI: 10.14334/jitv.v12i3.483

Abstract

The urinary excretion of purine derivative (PD), which consists of allantoin, uric acid, xanthine and hypoxanthine, was used to estimate Microbial Nitrogen (MN) supply on sheep given oaten hay as basal diet supplemented either with faba beans (faba) or barley fortified with urea (barley-urea). Three growing wethers, with average body weight of 43 ± 2.29 kg, were randomly assigned to one of three experimental diets, i.e. oaten hay without supplement (C), diet C + barley-urea (CB), and diet C + faba (CF), according to latin square design (3 x 3). Results of the experiment indicated that PD excretion for C was lower (P = 0.07) than that of CB and CF (2.84 vs 6.08, 7.67 mmol/d). Absorbed PD and estimation of MN supply for C was less (P = 0.07) than those of CB and CF, namely 1.26 vs 6.64, 8.80 mmol/d and 0.91 vs 4.8, 6.30 g N/d respectively. However, the efficiency of MN synthesis was similar, whether the calculation was based on digestible organic matter fermented in the rumen, DOMR (g MN/kg DOMR, P = 0.20) or based on N intake (g MN/g N intake, P = 0.40). In conclusion, supplementation of oaten hay with different protein supplements may improve the estimation of microbial N supply in the growing sheep but both supplements gave similar results on the estimation of MN supply. Key Words: Purine Derivative, Microbial Protein, Protein Supplement, Sheep
Rumen microbial protein supply as estimated from purine derivative excretion on sheep receiving faba beans (vicia faba) as supplement delivered at different feeding frequencies Asmuddin Natsir
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.582 KB) | DOI: 10.14334/jitv.v13i2.602

Abstract

Rapid and extensive degradation of faba beans (Vicia faba) by ruminal microbes can result in substantial and undesirable N loss from the rumen. The purpose of this study was to test the hypothesis that offering faba beans as a supplement more than once a day to sheep receiving a combination of oaten chaff and lucerne chaff as a basal diet will increase microbial protein supply to the intestines. The experiment was conducted in a Latin square design (4 x 4) using four mature merino sheep. The treatments were: T0 = basal diet ad libitum + nil supplements, T1 = T0 + faba beans (FB) fed once daily, T2 = T0 + FB fed twice daily, T3 = T0 + FB fed 8 times daily. The basal diet was given once per day at 09:00 in the morning while FB were given at the rate of approximately 0.5% of live body weight and delivered according to the treatment protocol. Urinary excretion of purine derivative (PD) was used to estimate microbial protein supply. The results indicated that even though treatment statistically had no effects on total urine output, PD excretion in the urine, PD absorbed, estimated microbial N supply, and the efficiency of rumen microbial protein synthesis, provision of supplement to sheep numerically improved microbial N supply by 92% compared to that of control group. However, there were no differences within the supplemented group. Therefore, it is concluded that feeding faba beans more than once a day was unnecessary. Key Words: Purine Derivatives, Microbial Protein Synthesis, Sheep
The effects of microwave radiation on rumen degradation characteristics of barley straw cut at two different stages of maturity Asmuddin Natsir
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.149 KB) | DOI: 10.14334/jitv.v12i2.548

Abstract

A common approach for improving the nutritive value of low quality roughages and crop by-products is by pre-treatment or processing either physical, chemical, or biological treatments. Microwave radiation is one type of physical treatment that could be used to treat low quality roughages. Research was carried out to investigate  the effects of microwave radiation on the rumen degradation characteristics of barley straw obtained from two different stages of maturity. The experiment was run factorially based on completely randomized block design. The first factor was stage of maturity, straw cut during the soft elongation time (C1) and during the harvest time (C2). The second factor was levels of microwave radiation times (MWRT) (T0 = control, without MWR; T1 = MWR for 1 minute, T2 = MWR for 2 minutes). The results indicated nutritive values of barley straw obtained from C1 sampling time were significantly better than that obtained from the C2 sampling time in terms of a higher rumen degradation rate and a much greater total potential rumen degradability. In contrast, MWR did not have significant effects on the rate of degradation and total potential degradability of straw in the rumen. Key Words: Microwave Radiation, Barley Straw, Rumen Degradability
PREPARASI LARUTAN FOSFAT DAN UREA MINERAL MOLASES LIQUID (UMML) SEBAGAI PENYEDIA PREKURSOR BIOFERMENTASI RUMEN Syahriani Syahrir; Asmuddin Natsir; Muhammad Zain Mide; Rohmiyatul Islamiati; Anie Asriani
Buletin Nutrisi dan Makanan Ternak Vol. 9 No. 1 (2013)
Publisher : Departemen Nutrisi dan Makanan Ternak, Fakultas Peternakan, Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.147 KB) | DOI: 10.20956/bnmt.v9i1.905

Abstract

Urea Mineral Molases Liquid (UMML) dapat menjadi prekursor biofermentasi dalam sistem rumen. UMML dapat menyediakan nitrogen lepas lambat, mineral larut air dan readily available carbohydrate (RAC),  namun memerlukan kajian khusus, terutama pada penentuan jenis dan preparasi bahan penyusunnya, khususnya penyediaan mineral fosfat larut air.  Tujuan penelitian ini adalah menghasilkan formula UMML yang selanjutnya akan digunakan sebagai prekursor biofermentasi rumen.  Beberapa perlakuan terhadap super fosfat (SP36) dilakukan untuk mendapatkan metode preparasi yang menghasilkan fosfat terlarut dengan konsentrasi yang tinggi. Preparasi yang menghasilkan kadar fosfat terlarut yang tertinggi adalah super fosfat yang di tambah asam organik dan larutan urea lalu dididihkan selama 5 menit. Urea Mineral Molases Liqiud diramu dari bahan Larutan Ca(Urea)4Cl2 + Larutan fosfat + Larutan NaCl jenuh + Molases. Kata Kunci:  Preparasi, Larutan fosfat, Urea Mineral Molases Liquid.
PENGARUH PENAMBAHAN BERBAGAI LEVEL TEPUNG DAUN KATUK (Sauropus Androgynus) TERHADAP KADAR KOLESTEROL, TRIGLISERIDA, LDL DAN HDL DARAH BROILER . Kamalia; Andi Mujnisa; Asmuddin Natsir
Buletin Nutrisi dan Makanan Ternak Vol. 10 No. 1 (2014)
Publisher : Departemen Nutrisi dan Makanan Ternak, Fakultas Peternakan, Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.445 KB) | DOI: 10.20956/bnmt.v10i1.909

Abstract

Percobaan ini bertujuan untuk mengetahui pengaruh penambahan berbagai tingkat daun katuk terhadap  kadar kolesterol, trigliserida, LDL, dan HDL dalam darah ayam broiler. Enam puluh empat (64) ekor  DOC berjenis kelamin campur, dengan metode rancangan acak lengkap. Ransum perlakuan adalah: P0 = contol, P1 = kontrol + 1% tepung daun katuk, P2 = Kontrol + 2% daun katuk makan,     P3 = kontrol + 3% tepung daun katuk. Perlakuan berjumlah 4, sehingga total perlakuannya menjadi 16. Ransum kontrol terdiri dari jagung kuning, minyak kelapa, limbah udang, tepung ikan, ampas tahu, kacang kedelai. Selama percobaan ayam broiler diberi pakan dan minum secara adlibitung. penelitian menunjukkan bahwa penambahan tepung daun katuk ke dalam pakan tidak mempengaruhi konsentrasi kolesterol, trigliserida, dan LDL dalam darah ayam broiler. Tetapi secara signifikan meningkatkan konsentrasi HDL darah tetapi tidak memiliki efek pada konsentrasi kolesterol darah, trigliserida darah, dan LDL darah ayam broiler. Kata kunci: Tepung Daun Katuk, Kolesterol, HDL
DAYA CERNA PROTEIN KASAR, LEMAK KASAR DAN SERAT KASAR RANSUM KOMPLIT MENGANDUNG BAHAN UTAMA TUMPI JAGUNG FERMENTASI PADA TERNAK KAMBING KACANG Ayu Antisa; asmuddin Natsir; syahriani syahrir
Buletin Nutrisi dan Makanan Ternak Vol. 14 No. 2 (2020)
Publisher : Departemen Nutrisi dan Makanan Ternak, Fakultas Peternakan, Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.339 KB) | DOI: 10.20956/bnmt.v14i2.12548

Abstract

Daya cernaan bahan pakan adalah bagian zat yang dapat dicerna oleh tubuh dan diasumsikan diserap oleh tubuh ternak sehingga tidak diekskresikan dalam feses. Jumlah maupun komposisi kimia serat suatu bahan pakan sangat berpengaruh terhadap kecernaannya. Tujuan penelitian ini untuk mengevaluasi daya cerna protein, daya cerna lemak, dan daya cerna serat pakan komplit mengandung tumpi jagung fermentasi pada ternak kambing kacang. Penelitian ini menggunakan Sepuluh ekor ternak kambing kacang secara random dibagi kedalam dua kelompok perlakuan (5 ekor/kelompok). Kelompok (F) diberi pakan komplit mengandung tumpi fermentasi dan pada kelompok (TF) diberi pakan komplit mengandung tumpi tanpa fermentasi. Hasil penelitian menunjukkan bahwa kedua perlakuan tidak berpengaruh nyata (P>0,05) terhadap daya cerna protein, daya cerna lemak dan daya cerna serat kasar pakan. Kesimpulan Pemberian ransum komplit tumpi jagung fermentasi dan ransum komplit tumpi jagung tanpa fermentasi kecernaan protein dari kedua perlakuan cukup tinggi yaitu berada di angka diatas 70% begitupula dengan kecernaan lemak juga cukup tinggi yaitu rataan diatas 80% dan kecernaan serat rataan diatas 50%.
Potensi Bakteri Endofit Dari Zea mays L. Sebagai Penghasil Fitase Hafsan Hafsan; Nurhikmah Nurhikmah; Yuniar Harviyanti; Eka Sukmawaty; Isna Rasdianah Aziz; Cut Muthiadin; Laily Agustina; Asmuddin Natsir; Ahyar Ahmad
Prosiding Seminar Biologi Vol 3 No 1 (2017): Prosiding Seminar Nasional Biology for Life
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v3i1.4676

Abstract

Asam fitat (C6H18O24P6) merupakan senyawa kimia yang terdiri atas inositol dan asam fosfat, asam fitat merupakan senyawa yang selalu terdapat pada bahan pakan yang berasal dari tanaman serealia dan merupakan senyawa yang tidak dapat didigesti oleh ternak monogastrik, sehingga dibutuhkannya enzim fitase yang dapat menghidrolisis asam fitat. Fitase (myo-inositol heksakisfosfat fosfohidrolase) adalah enzim yang dapat menghidrolisis ikatan fosfoester pada asam fitat, menghasilkan fosfat anorganik dan ester fosfat. Enzim fitase dapat dihasilkan oleh mikroorganisme, salah satunya adalah bakteri endofit. Bakteri endofit adalah mikroorganisme yang menguntungkan yang berinteraksi dengan tanaman inang tanpa menyebabkan gangguan atau kerusakan pada tanaman tersebut, pada penelitian ini bakteri endofit telah diisolasi dari tanaman jagung (Zea mays) yang digunakan sebagai sampel penelitian. Isolasi dilakukan dari masing-masing organ tanaman jagung (Zea mays) dan diseleksi menggunakan media selektif PSM (Phytase Screening Media). Empat isolat yang terpilih dari masing-masing organ berdasarkan IF (Indeks Fitatik) tertinggi kemudian diidentifikasi molekuler dan memperoleh hasilisolat akar 10-7 HF.7 (IF=1,365 cm) merupakan bakteri Burkholderia lata, batang 10-7 HF.2 (IF=1,095 cm) merupakan bakteri Pantoea stewarti subsp. indologens, daun 10-6 HF.3 (IF=1,36 cm) merupakan bakteri Enterobacter ludwigi dan biji 10-8 HF.1 (IF=0,98 cm) merupakan bakteri Enterobacter cloaceae.
Stabilitas Aktivitas Fitase Dari Bukholderia lata Strain HF.7 Hafsan Hafsan; Laily Agustina; Asmuddin Natsir; Ahyar Ahmad
Prosiding Seminar Biologi Vol 3 No 1 (2017): Prosiding Seminar Nasional Biology for Life
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v3i1.4678

Abstract

Penelitian ini bertujuan untuk mengetahui stabilitas fitase ekstraseluler Bukholderia lata strain HF.7 terhadap suhu, pH, dan aktivitas protease sebagaimana kondisi saluran pencernaan unggas pada umumnya. Uji stabilitas suhu dilakukan dengan memaparkan pada suhu yang bervariasi (20, 30, 37, 40, 45, 50, 55, 60, dan 65oC), sedangkan uji stabilitas pH dilakukan dengan cara menginkubasi fitase pada buffer Na-asetat dengan pH bervariasi (1, 2, 3, 4, 5, 6, 7, 8). Uji stabilitas fitase terhadap protease dilakukan dengan penambahan protease dengan level perlakuan P0: tanpa protease, P1: penambahan pepsin (5000 unit/ mL), P2: penambahan pepsin (5000 unit/ mL) dan P3: penambahan pepsin + tripsin (@2500 unit/ mL) dengan Rancangan Acak Lengkap. Nilai aktivitas fitase pada suhu dan pH dilakukan dengan menggunakan analisis deskriptif dengan mengamati nilai rata-rata dari aktivitas fitase. Hasil penelitian menunjukkan bahwa fitase ekstraseluler Bukholderia lata strain HF.7 memiliki aktivitas yang lebih baik pada kemurnian yang lebih tinggi serta optimum pada pH 4 dan suhu 37oC. Fitase yang dihasilkan juga aktivitas yang stabil pada pH maupun suhu serta protease (pepsin dan tripsin) sebagaimana kondisi saluran pencernaan unggas pada umumnya. Temuan ini menunjukkan prospek potensial fitase ekstraseluler Bukholderia lata strain HF.7 yang dapat diaplikasikan pada pakan unggas untuk meningkatkan produktivitas.
The Physicochemical Properties of Goat Skin Gelatin at Different Ages With the Use of Lactobacillus plantarum and Acetic Acid as a Pretreatment Hasma Hasma; Effendi Abustam; Ratmawati Malaka; Muhammad Irfan Said; Asmuddin Natsir
Hasanuddin Journal of Animal Science (HAJAS) Vol. 1, No. 2 (2019)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.v1i2.8150

Abstract

ABSTRACT Pretreatment of chemical acids using organic chemicals has been widely applied, while pretreatment using microbial Lactobacillus plantarum to form ossein in goat skin has not been done much. Application of Lactic Acid Bacteria (LAB) microbial species L. plantarum is thought to affect health and environment friendly. This study aims to determine the characteristics of goat skin gelatin at different ages of L. plantarum 1uhcc pretreatment and acetic acid (CH3COOH). Each treatment was repeated 3 times. The study design used a Split Plot Design with acid source treatment as the main plot and the age of goats 1, 2 and 3 years as subplots. The results showed a very significant effect (P<0.01) between the age of the goat with gel strength, pH, viscosity and color. The highest gel strength and viscosity at 1 year of age with L.plantarum pretreatment respectively (441.37±0.78 g bloom) and (9.22±0.09 cP). Goat skin gelatin has the highest pH at 1 year of age with L.plantarum 5.24±0.16. Similarly, the effect of gelatin (P<0.05) with a brightness level (L) 76.38±1.10 and redness (a) 11.13±1.58 affect the age of goats and acidic sources while yellowish (b) 33.81±2.34 only affects the acid source. Age of goats with different acid pretreatment has an impact on the physicochemical properties of goat skin gelatin. Goat age 1 year with L.plantarum pretreatment showed optimal results.
Consumption and Digestibility Studies in Goats Fed with Complete Feed Corn Tumpi and Red Dragon Fruit Peel Flour (Hylocereus Sp.) Mariam, Mariam; Natsir, Asmuddin; Syahrir, Syahriani
Hasanuddin Journal of Animal Science (HAJAS) Vol. 5 No. 1 (2023)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.v5i1.21987

Abstract

Utilization of unconventional materials such as corn tumpi and dragon fruit peel flour as an alternative feed ingredient for ruminants, especially goats. This study aims to examine the effect of providing complete feed containing corn tumpi with dragon fruit peel flour at different levels on consumption and digestibility of goats. This study used 12 local male goats with ages ranging from 1 to 1.5 years which were randomly assigned to metabolic cages. The study used a Randomized Block Design (RBD) method with 3 treatments and 4 groups. The treatments consisted of P1: Complete feed containing 50% corn tumpi, P2: Complete feed containing 45% corn tumpi and 5% dragon fruit peel flour and P3: Complete feed containing 40% corn tumpi and 10% dragon fruit peel flour. The results showed that the treatment of complete feed containing corn tumpi with dragon fruit peel flour at different levels had a significant effect (P<0.05) on dry matter consumption and organic matter consumption. Dry matter consumption ranged from 296.88 – 461.64 g/h/day, and nitrogen consumption was between 265.74 – 407.72 g/h/day. Treatment of complete feed containing corn tumpi with dragon fruit peel flour at different levels had no significant effect (P>0.05) on dry matter digestibility and organic matter digestibility. Dry matter digestibility ranged from 82.75 – 87.50 %, and organic matter digestibility between 84.5 – 88.75 %. It was concluded that based on estimates of dry matter consumption and organic matter consumption, the use of alternative ingredients for corn tumpi with dragon fruit peel flour up to a level of 10% could be used as a complete mix of feed ingredients for ruminants, especially goats.
Co-Authors . Kamalia . Kamalia, . A. Nurhayu Abd. Rasyid Syamsuri Ahyar Ahmad Anas, Serli Andi Baso L. Ishak, Andi Baso L. Andi Mujnisa Angga Nugraha Ani Asrianie, Ani Anie Asriani Anie Asriani, Anie Antonius Ayu Antisa Cut Muthiadin Dagong, Muh. Ihsan A. Effendi Abustam Fitri, Yelly Hafsan, Hafsan Hakim, Mita Arifa Hakim Haris, Aslizah Mohd. Hasma Hasma Hidayat, M. Zhorif Idrus, Izdin Ikhlas, Zaitul Isna Rasdianah Aziz Laily Agustina Laily Agustina, Laily Mansur, Musdalifa Mardiati Zain Mariam Mariam Mashuri Masri Maslan, Muhammad Matheus Sariubang Mide, M. Zain Mide, M. Zain Mohd-Aris, Aslizah Muhammad Irfan Said Muhammad Zain Mide Muhammad Zain Mide Nurhikmah Nurhikmah Nurzainah Ginting Purnomo, Nurul Rahman, Taufica Rahmat Hidayat Ratmawati Malaka Rohmiyatul Islamiati Rohmiyatul Islamiati, Rohmiyatul Rohmiyatul Islamiyati, Rohmiyatul Roni Pazla Sucitra, Laras Sukma Sukmawaty, Eka Susilawati, Susilawati Syahdar Baba Syahriani Syahrir Syahriani Syahrir Syahriani Syahrir Syamsuddin Nompo, Syamsuddin Urip Rosani Utami, Bella Veliana Yanti, Gusri Yuniar Harviyanti