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All Journal JPMS (Jurnal Pendidikan Matematika dan Sains) Jurnal Penelitian Saintek Jurnal Penelitian Humaniora INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni Ganendra: Majalah IPTEK Nuklir Jurnal Sains Dasar Biotropika Indonesian Journal of Biotechnology Scientiae Educatia: Jurnal Pendidikan Sains Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Journal of Education Technology Jurnal Abdi Insani Jurnal Manajemen Pendidikan dan Ilmu Sosial (JMPIS) Borobudur Jurnal Natural Kingdom (The Journal of Biological Studies) IJOBI Jurnal Pendidikan Progresif
Bernadetta Octavia
Department Of Biology Education, Faculty Of Mathematics And Natural Sciences, Universitas Negeri Yogyakarta, Yogyakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Neuroscience Physics Public Health Social Sciences Other

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PEMBERDAYAAN MASYARAKAT DALAM PENGELOLAAN LIMBAH ORGANIK MENJADI EKOENZIM MULTI GUNA DAN RAMAH LINGKUNGAN Suhartini, Suhartini; Octavia, Bernadetta; Aminatun, Tien; Aulia, Fera
Jurnal Abdi Insani Vol 12 No 2 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i2.2313

Abstract

Masalah sampah merupakan isu besar, terutama dengan ditutupnya Tempat Pemrosesan Akhir (TPA) Piyungan dalam menampung sampah dari Kabupaten Sleman, Bantul, dan Kota Yogyakarta. Di Padukuhan Nayan, sampah organik dan anorganik dibuang di pekarangan dan di sungai atau dibakar oleh sebagian warga sehingga menimbulkan pencemaran lingkungan dan mengganggu Kesehatan. Masyarakat belum mempunyai pengetahuan mengelola sampah organic menjadi produk yang bermanfaat dan ramah lingkungan, padahal sampah organik berpotensi dimanfaatkan menjadi produk bermanfaat dan bisa bernilai ekonomi. Maka dari itu kegiatan ini bertujuan untuk  mengedukasi dan melatih masyarakat dalam mengelola sampah organik menjadi ekoenzim yang bermanfaat dan ramah lingkungan. Kegiatan dilakukan melalui sosialisasi, pemberian materi, praktek pembuatan dan pemanfaatan ekoenzim, monitoring dan evaluasi kegiatan kepada 45 peserta yang terdiri dari warga pedukuhan Nayan. Pelatihan dilakukan pada 7 September 2024, dengan pemantauan setiap  bulan sekali selama 3 bulan. Peserta menunjukkan antusiasme yang tinggi terhadap materi dan praktek pembuatan ekoenzim yang dapat dilihat dari penilaian evaluasi oleh peserta dengan hasil sangat baik. Setelah pelatihan, mereka berhasil mengaplikasikan teknik pembuatan ekoenzim dalam mengelola sampah organik rumah tangga. Ekoenzim yang dihasilkan digunakan untuk pupuk dan pembersih lantai. Kegiatan ini juga menunjukkan peningkatan kesadaran tentang pentingnya pengelolaan sampah secara ramah lingkungan, yang mendukung tujuan SDGs ke-12 terkait produksi dan konsumsi yang bertanggung jawab. Pelatihan pembuatan ekoenzim berhasil meningkatkan keterampilan masyarakat dalam pengelolaan sampah organik dan berkontribusi pada pengelolaan lingkungan sekitar menuju pembangunan berkelanjutan.
POTENSI PENGGUNAAN KONSORSIUM ISOLAT BAKTERI PLASTIPHARE DARI PANTAI SADRANAN, GUNUNGKIDUL SEBAGAI AGEN BIODEGRADASI HIGH DENSITY POLYETHYLENE Saladina, Salma Putri; Octavia, Bernadetta
Kingdom: The Journal of Biological Studies Vol 10, No 2 (2024): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v10i2.23218

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan efektifitas penggunaan konsorsium bakteri plastisphere dibandingkan dengan isolat tunggal dalam mendegradasi plastik HDPE. Perbedaan efektifitas diukur berdasarkan analisis pengurangan berat plastik, analisis bakteri permukaan plastik dan analisis permukaan menggunakan Scanning Electron Microscope (SEM). Penelitian ini termasuk dalam jenis eksperimen dengan proses isolasi bakteri pada sampah plastik yang ditemukan pada Pantai Sadranan, Gunungkidul, diseleksi berdasarkan kemampuan enzimatik lignolitik serta tiga isolat terbaik diuji kemampuan biodegradasinya dalam bentuk konsorsium serta isolat tunggal. Hasil penelitian menunjukkan adanya persentase degradasi tertinggi sebesar 7,14% ± 0,59 ditunjukkan oleh perlakuan konsorsium sedangkan perlakuan isolat tunggal H5, H9, dan H10 secara berturut sebesar 1,55% ± 0,5, 5,62% ± 0,19, dan 3,11% ± 0,49. Tiga bakteri plastisphere dikarakterisasi dan identifikasi dan menunjukkan bahwa terdapat 2 isolat dengan indeks similaritas ≥ 80% terhadap acuan Arthrobacter sp. dan satu isolat dengan similaritas 68% terhadap acuan Micrococcus sp. Berdasarkan hasil yang diperoleh, penggunaan konsorsium bakteri sebagai agen biodegradasi efektif dalam mendegradasi plastik HDPE dibandingkan dengan penggunaan isolat tunggal.
Isolation and Identification of Profenofos Pesticide-Tolerant Bacteria from Agriculture Land in Sleman Regency Pertiwi, Melania Citra; Rakhmawati, Anna; Octavia, Bernadetta; Aminatun, Tien; Suhartini
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

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Abstract

The intensive application of organophosphate pesticides containing profenofos as the active compound may lead to soil contamination and the emergence of pesticide-tolerant bacterial strains with potential for bioremediation. Comprehensive identification and characterization of these bacteria are crucial to support sustainable agricultural and environmental management practices. The aim of the study is to analyze the characteristics and identify indigenous bacteria tolerant to profenofos pesticide in Sleman agricultural land, DIY. This study is qualitative research using an exploratory-descriptive. Isolation was carried out by dilution to a level of 10-7 followed by a spread plate on Mineral Salt Medium (MSM) which was given with 100 ppm profenofos pesticide. Bacterial isolates were characterized and then identified using profile matching with reference genera. Based on the results of the study, it is found that 10 bacterial isolates tolerant to organophosphate pesticide with the active ingredient profenofos that have a similarity index of ≥70%. Based on the reference bacteria, 5 different genera are obtained, namely Paracoccus (D3, D8), Micrococcus (D5, D6, D9), Enterococcus (D2, D10), Arthrobacter (D1, D4), and Neisseria (D7). The bacterial isolates obtained have different characteristics. The characteristics of these bacteria are gram positive and negative bacteria with round, irregular, and rhizoid shapes. Their margins are lobate, curled, entire, undulate, and ciliate. Their elevations are umbonate, raised, and flat. The color of the bacteria is white to light orange, with various physiological test results.
Selection of Cellulolytic Bacteria Found in Mask Waste from Wana Tirta Mangrove Pramesti, Ariska Hanum; Octavia, Bernadetta
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

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Abstract

The amount of mask waste has increased significantly due to the COVID-19 pandemic. Mask waste can be found everywhere, likewise in Wana Tirta Mangrove. Its existence in the mangrove ecosystem, making mask waste, has the potential for cellulolytic bacteria. The purpose of this study is to determine the cellulase enzyme activity, characteristics, and genus of purified cellulolytic bacteria found in mask waste from Wana Tirta Mangrove. The method used in this study was qualitative research. The samples for this research were bacterial isolates from mask waste, which had previously been isolated from the Wana Tirta Mangrove. Samples were grown on CMC media to determine isolates that were included in cellulolytic bacteria. The cellulase activity of cellulolytic bacteria was tested qualitatively and quantitatively. Morphological, physiological and biochemical tests were carried out to obtain phenetic characters which were then used as the basis for determining the genus of cellulolytic bacteria. The results showed that 10 isolates were cellulolytic bacteria. The highest activity of cellulase enzyme qualitatively was produced by isolate 72 with a value of 0.466 mm, while the highest activity of cellulase enzyme quantitatively was produced by isolate 84 with a value of 0.931 U/ml. The dendrogram result showed 4 genera of cellulolytic bacteria, including Pseudomonas, Alcaligenes, Cellulomonas, and Aeromonas.
MOLECULAR IDENTIFICATION OF A LOCAL LACTIC ACID BACTERIA (LAB) ISOLATE (B21) AND PRIMER CONFIRMATION FOR D-Lactate Dehydrogenase (D-LDH) GENE ISOLATION Madhani, Anisa Tiara; Nurcahyo, Heru; Octavia, Bernadetta; Astuti; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.104

Abstract

This study aims to identify a lactic acid bacteria (BAL) isolated from native chicken (Gallus gallus domesticus) intestinum (B21) based on 16S rRNA gene sequence and also to confirm primers for PCR-based D-Lactate dehydrogenase (D-LDH) gene isolation. The genomic DNA of B21 was isolated then amplified using 16S rRNA. The PCR product then sequenced and aligned. The sequencing was done in 1st Base Pte. Malaysia. The sequence then aligned using the Basic Local Alignment Search Tool (BLAST) in NCBI to know the similar genome species. Isolation D-LDH gene was done through PCR using 5 primers (idb 0101, idb 1147, idb 0813, idb 1010, and idb 2021) of B21 and also J15 isolate genome. The PCR product were then sequenced and aligned with the D-LDH gene sequences in NCBI. The results of molecular identification based on 16S rRNA markers showed that B21 has 99.45% similarity to Bacillus proteolyticus. Amplification of the LDH gene with idb 1010 primer on J15 isolate yielded 4 fragments (i.e. 650, 1400, 1500, and 1700bp). The results of phylogenetic tests showed that the 1700bp fragment has a high similarity to the D-LDH gene in Myobacterium tuberculosis species with a bootstrap value of 84%. While other size fragments cannot be trusted to have similarity to LDH sequence because they have not reached the minimum bootstrap value that meets the qualifications.
Antibacterial Activity of Green Meniran Extract (Phyllanthus niruri Linn) on The Growth of Salmonella typhimurium Nafisah, Aulia Erta; Octavia, Bernadetta; Lestari, Endang Gati
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.215

Abstract

Salmonella typhimuriumis a bacterium that causes digestive infections, gastroenteritis, and food poisoning, psickcaused byBacterial infection is a health problem in developing countries, including Indonesia. Green meniran (Phyllanthus niruri L.), is a herbaceous plant containing flavonoids, alkaloids, saponins, and tannins which have antibacterial activity which is expected to suppress the development of Salmonella sp. The aim of the study was to determine the ability of green meniran extract as an antibacterial to inhibit the growth of Salmonella typhimurium bacteria. The study used a completely randomized factorial design consisting of two factors, namely extract concentrations of 5%, 10%, 20%, 40%, 80%, equipped with a positive control (chloramphenicol) and negative control (aquades), as well as the age of the inoculum of the bacterial growth phase. Testing the antibacterial activity using the Kirby-Bauer disc diffusion method. The results showed that green meniran extract had antibacterial activity against the growth of Salmonella typhimurium bacteria. Concentrations of 5%, 10%, 20%, 40%, and 80% produced inhibition zone diameters of 6.7 mm, 8.5 mm, 9.9 mm, 12 mm, and 14.6 mm, respectively. The concentration of 80% green meniran extract is effective in inhibiting the growth of Salmonella typhimurium bacteria with an antibacterial effectiveness value of 58.95%.
The Effect of Encyclopedia and Discovery Learning to Improve Learning Motivation and Mastery of Concepts in Student Anggraeni, Neli Dwi Septi; Octavia, Bernadetta
Jurnal Pendidikan Progresif Vol 13, No 3 (2023): Jurnal Pendidikan Progresif
Publisher : FKIP Universitas Lampung

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Abstract

The Effect of Encyclopedia and Discovery Learning to Improve Learning Motivation and Mastery of Concepts in Student. Objectives: This study aims to determine effectiveness of encyclopedia structure function of plant tissue and discovery learning to increase learning motivation and mastery of concepts in grade XI MA. Methods: Techniques of data collection in learning motivation with questionnaires and mastery of concepts using tests. Tests and questionnaires were collected twice, namely pretest and posttest. The data obtained carried out prerequisite test analysis with normality and homogeneity test. Furthermore, paired t-test with SPPS V.15. Findings: The results of statistical analysis showed that P-value<0.05 that the encyclopedia structure function of plant tissue and discovery learning was effective in increasing student learning motivation and increasing mastery of concepts in grade XI MA. Conclusion: Effectiveness of encyclopedia structure function of plant tissue and discovery learning can improve learning motivation with effective categories and effectiveness of encyclopedia structure function of plant tissue and discovery learning can improve mastery of concepts with effective categories. Keywords: discovery learning, encyclopedia, learning motivation, mastery of concepts.DOI: http://dx.doi.org/10.23960/jpp.v13.i3.202334
EFEK PENGHAMBATAN EKSTRAK DAUN BUNI (Antidesma bunius (L.) Spreng) TERHADAP PERTUMBUHAN Candida albicans Nofia, Fifi; Octavia, Bernadetta
Kingdom (The Journal of Biological Studies) Vol 11, No 2 (2025): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v11i2.19789

Abstract

Tanaman buni (Antidesma bunius (L.) Spreng) memiliki khasiat obat dan digunakan secara tradisional. Selain itu, bagian daun tumbuhan diketahui memiliki potensi sebagai antimikroba terutama bakteri. Tantangan lain sebagai agen antimikroba adalah adanya fungi. Salah satu fungi yang banyak menyebabkan penyakit pada manusia adalah Candida albicans. Pengobatan infeksi C. albicans biasanya menggunakan bahan kimia antifungi yang dapat menimbulkan efek samping sehingga dibutuhkan alternatif pengobatan lain yang lebih aman. Tujuan penelitian ini adalah untuk mengetahui kemampuan ekstrak daun buni (Antidesma bunius (L.) Spreng) dalam menghambat pertumbuhan Candida albicans. Penelitian ini merupakan penelitian eksperimen dengan menggunakan dua pelarut ekstrak yaitu etanol 96% dan metanol. Konsentrasi ekstrak yang digunakan adalah 0,2:1; 0,4:1; 0,6:1; 0,8:1 gram/ml dengan kontrol negatif (DMSO 1%) dan kontrol positif (Ketokonazol dan Nystatin). Hasil penelitian menunjukkan bahwa daya hambat ekstrak etanol 96% dan metanol daun buni tidak terlalu berbeda. Penghambatan kedua ekstrak terbatas pada konsentrasi ekstrak 0,8:1 dan pada pengamatan jam ke-12 masa inkubasi penelitian. Efektivitas penghambatan ektrak daun buni tidak mencapai 50% dibandingkan dengan antibiotik Ketokonazol dan Nystatin 1000 ppm. Dari hasil ini diketahui bahwa ekstrak daun buni tersebut tidak efektif dalam menghambat pertumbuhan Candida albicans.
Pengembangan E-Modul Problem Based Learning dengan Socio-Scientific Issues (SSI) tentang HIV-AIDS untuk Meningkatkan Motivasi Belajar dan Literasi Kesehatan Reproduksi Peserta Didik Kelas XI Irenne Odly Kathleen Pasassung; Bernadetta Octavia
Jurnal Manajemen Pendidikan dan Ilmu Sosial Vol. 7 No. 1 (2025): Jurnal Manajemen Pendidikan dan Ilmu Sosial (Desember 2025 - Januari 2026)
Publisher : Dinasti Review

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38035/jmpis.v7i1.6854

Abstract

Latar belakang dari pengembangan ini adalah rendahnya motivasi belajar dan literasi kesehatan reproduksi peserta didik akibat keterbatasan bahan ajar yang kontekstual, interaktif, dan relevan. Oleh karena itu, penelitian ini bertujuan untuk mengembangkan E-Modul Problem Based Learning (PBL) dengan pendekatan Socio-Scientific Issues (SSI) pada materi sistem reproduksi tentang HIV-AIDS untuk meningkatkan motivasi belajar dan literasi kesehatan reproduksi peserta didik kelas XI di Serui, Papua. Penelitian ini mengintegrasikan model PBL untuk mendorong pemecahan masalah berbasis kehidupan nyata serta pendekatan SSI untuk membangun kesadaran peserta didik terhadap isu sosial yang berkaitan dengan materi pembelajaran. Penelitian ini merupakan penelitian dan pengembangan (Research and Development) menggunakan model ADDIE (Analysis, Design, Development, Implementation, Evaluation). Subjek penelitian ini adalah peserta didik kelas XI MIPA 2 dan MIPA 6 di SMA Negeri 1 Serui tahun ajaran 2024/2025. Teknik pengumpulan data meliputi wawancara, angket, dan tes. Instrumen yang digunakan telah divalidasi oleh ahli materi, ahli media, dan praktisi (guru dan peserta didik), yang menunjukkan kategori “sangat valid” untuk aspek kelayakan isi, media, dan bahasa. Uji kepraktisan dilakukan dengan menyebarkan angket kepada guru dan peserta didik, sedangkan uji keefektifan dilakukan melalui pretest dan posttest menggunakan analisis MANOVA, setelah sebelumnya diuji normalitas, homogenitas, dan multikolinieritas. Hasil penelitian menunjukkan bahwa E-Modul PBL dengan SSI yang dikembangkan valid, praktis, dan efektif. Validitas media dan instrumen penelitian mencapai skor lebih dari 90% yang dikategorikan “sangat valid”. Kepraktisan menurut peserta didik dan guru juga sangat tinggi dengan skor di atas 90. Analisis MANOVA menunjukkan bahwa terdapat perbedaan signifikan antara kelas eksperimen dan kontrol dalam hal motivasi belajar dan literasi kesehatan reproduksi, yang mengindikasikan bahwa E-Modul efektif dalam meningkatkan kedua variabel tersebut. Berdasarkan hasil tersebut, disarankan agar guru biologi di wilayah dengan tantangan kontekstual seperti Papua memanfaatkan E-Modul ini sebagai bahan ajar tambahan yang relevan dan aplikatif dalam mendukung pembelajaran kontekstual dan meningkatkan kesadaran peserta didik terhadap isu- isu sosial yang aktual.
Characterization And Identification of Halophilic Bacteria (Rastrelliger sp.) Extracellular Lipase Generator in Beringharjo Market Octavia, Bernadetta; Rakhmawati, Anna
Indonesian Journal of Bioscience (IJOBI) Vol. 2 No. 2 (2024): Indonesian Journal of Bioscience (IJOBI)
Publisher : Department of Biology Education FMIPA Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v2i2.1056

Abstract

Advances in biotechnology have allowed for the widespread use of lipase enzymes. One of the producers of lipase enzymes is halophilic bacteria. This study aims to determine the characteristics and genus, as well as the kinship relationship between lipase-producing halophilic bacterial isolates from salted fish (Rastrelliger sp.) in Beringharjo Market, Yogyakarta. Salted fish samples were isolated on Nutrient Agar + 3% NaCl + Tween 80 + 0.1% Rhodamine-B selective media to grow lipase-producing halophilic bacteria. Identification was carried out by the matching profile method based on Bergey's Manual of Determinative Bacteriology. The kinship relationship between bacterial isolates was classified using MVSP 3.1 software with the UPGMA clustering algorithm and constructed in the form of a dendogram.
Co-Authors Abd. Rasyid Syamsuri Adinda Yuslia Rukmanandita Agus Taftazani Agus Taftazani Agus Taftazani Aminatun, Tien Anggraeni, Neli Dwi Septi Anna Rakhmawati Aris Bastianudin Astuti Astuti Astuti Aulia, Fera Ayuningtyas, Dwi Endang Gati Lestari Fellary Pangesti Fita Nilasari H Yulipriyanto, H Hafizh Adyatma Hartanti, Oktavia Dwi Herlinawati, Nabila Heru Nurcahyo Heru Nurcahyo Imam Riadi Imtiyaz, Arifah Nuha Irenne Odly Kathleen Pasassung Isti'anah, Rayi Kuni Ixora Sartika Mercuriani Kun Sri Budiasih L Sembiring Madhani, Anisa Tiara Mohamad Habibi Nadya Welli Astuti Nafisah, Aulia Erta Nahar Cahyandaru Nahar Cahyandaru Neli Dwi Septi Anggraeni Nofia, Fifi Nugraha, Aditya Dirgantara Nur Rizki Putri Ramadhanti Nuradiah, Mawaddah Fatin Pertiwi, Melania Citra Pramesti, Ariska Hanum Pratama, Anggi Tias Pujiati TM, Indah Putri Nur Indahsari Putri Nuri Nirwana Raphasya, Fhadinda Hizza Retno Sandi Saputra Rina Rahmawati, Rina Rivaldo Alphrazy Rizka Apriani Putri Saladina, Salma Putri Sarah Rapma Kristina Sitanggang Sari, Herninda Dhama Siti Nur Azizah Siti Umniyatie Sudarsono Suhandoyo Suhartini Suhartini Suhartini Suhartini Suhartini Triwibowo Yuwono