Article Per Year (5 Year)
p-Index From 2021 - 2026
3.761
P-Index
This Author published in this journals
All Journal Jurnal Penelitian Saintek Jurnal Penelitian Humaniora INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni Ganendra: Majalah IPTEK Nuklir Jurnal Sains Dasar Biotropika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Journal of Education Technology Jurnal Abdi Insani Borobudur Jurnal Natural Kingdom (The Journal of Biological Studies) IJOBI Jurnal Pendidikan Progresif
Bernadetta Octavia
Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Neuroscience Physics Public Health Social Sciences Other

Published : 31 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 31 Documents
Search

 1   2   3   4 
POTENSI PENGGUNAAN KONSORSIUM ISOLAT BAKTERI PLASTIPHARE DARI PANTAI SADRANAN, GUNUNGKIDUL SEBAGAI AGEN BIODEGRADASI HIGH DENSITY POLYETHYLENE Saladina, Salma Putri; Octavia, Bernadetta
Kingdom: The Journal of Biological Studies Vol 10, No 2 (2024): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v10i2.23218

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan efektifitas penggunaan konsorsium bakteri plastisphere dibandingkan dengan isolat tunggal dalam mendegradasi plastik HDPE. Perbedaan efektifitas diukur berdasarkan analisis pengurangan berat plastik, analisis bakteri permukaan plastik dan analisis permukaan menggunakan Scanning Electron Microscope (SEM). Penelitian ini termasuk dalam jenis eksperimen dengan proses isolasi bakteri pada sampah plastik yang ditemukan pada Pantai Sadranan, Gunungkidul, diseleksi berdasarkan kemampuan enzimatik lignolitik serta tiga isolat terbaik diuji kemampuan biodegradasinya dalam bentuk konsorsium serta isolat tunggal. Hasil penelitian menunjukkan adanya persentase degradasi tertinggi sebesar 7,14% ± 0,59 ditunjukkan oleh perlakuan konsorsium sedangkan perlakuan isolat tunggal H5, H9, dan H10 secara berturut sebesar 1,55% ± 0,5, 5,62% ± 0,19, dan 3,11% ± 0,49. Tiga bakteri plastisphere dikarakterisasi dan identifikasi dan menunjukkan bahwa terdapat 2 isolat dengan indeks similaritas ≥ 80% terhadap acuan Arthrobacter sp. dan satu isolat dengan similaritas 68% terhadap acuan Micrococcus sp. Berdasarkan hasil yang diperoleh, penggunaan konsorsium bakteri sebagai agen biodegradasi efektif dalam mendegradasi plastik HDPE dibandingkan dengan penggunaan isolat tunggal.
Isolation and Identification of Profenofos Pesticide-Tolerant Bacteria from Agriculture Land in Sleman Regency Pertiwi, Melania Citra; Rakhmawati, Anna; Octavia, Bernadetta; Aminatun, Tien; Suhartini
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The intensive application of organophosphate pesticides containing profenofos as the active compound may lead to soil contamination and the emergence of pesticide-tolerant bacterial strains with potential for bioremediation. Comprehensive identification and characterization of these bacteria are crucial to support sustainable agricultural and environmental management practices. The aim of the study is to analyze the characteristics and identify indigenous bacteria tolerant to profenofos pesticide in Sleman agricultural land, DIY. This study is qualitative research using an exploratory-descriptive. Isolation was carried out by dilution to a level of 10-7 followed by a spread plate on Mineral Salt Medium (MSM) which was given with 100 ppm profenofos pesticide. Bacterial isolates were characterized and then identified using profile matching with reference genera. Based on the results of the study, it is found that 10 bacterial isolates tolerant to organophosphate pesticide with the active ingredient profenofos that have a similarity index of ≥70%. Based on the reference bacteria, 5 different genera are obtained, namely Paracoccus (D3, D8), Micrococcus (D5, D6, D9), Enterococcus (D2, D10), Arthrobacter (D1, D4), and Neisseria (D7). The bacterial isolates obtained have different characteristics. The characteristics of these bacteria are gram positive and negative bacteria with round, irregular, and rhizoid shapes. Their margins are lobate, curled, entire, undulate, and ciliate. Their elevations are umbonate, raised, and flat. The color of the bacteria is white to light orange, with various physiological test results.
Selection of Cellulolytic Bacteria Found in Mask Waste from Wana Tirta Mangrove Pramesti, Ariska Hanum; Octavia, Bernadetta
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The amount of mask waste has increased significantly due to the COVID-19 pandemic. Mask waste can be found everywhere, likewise in Wana Tirta Mangrove. Its existence in the mangrove ecosystem, making mask waste, has the potential for cellulolytic bacteria. The purpose of this study is to determine the cellulase enzyme activity, characteristics, and genus of purified cellulolytic bacteria found in mask waste from Wana Tirta Mangrove. The method used in this study was qualitative research. The samples for this research were bacterial isolates from mask waste, which had previously been isolated from the Wana Tirta Mangrove. Samples were grown on CMC media to determine isolates that were included in cellulolytic bacteria. The cellulase activity of cellulolytic bacteria was tested qualitatively and quantitatively. Morphological, physiological and biochemical tests were carried out to obtain phenetic characters which were then used as the basis for determining the genus of cellulolytic bacteria. The results showed that 10 isolates were cellulolytic bacteria. The highest activity of cellulase enzyme qualitatively was produced by isolate 72 with a value of 0.466 mm, while the highest activity of cellulase enzyme quantitatively was produced by isolate 84 with a value of 0.931 U/ml. The dendrogram result showed 4 genera of cellulolytic bacteria, including Pseudomonas, Alcaligenes, Cellulomonas, and Aeromonas.
MOLECULAR IDENTIFICATION OF A LOCAL LACTIC ACID BACTERIA (LAB) ISOLATE (B21) AND PRIMER CONFIRMATION FOR D-Lactate Dehydrogenase (D-LDH) GENE ISOLATION Madhani, Anisa Tiara; Nurcahyo, Heru; Octavia, Bernadetta; Astuti; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.104

Abstract

This study aims to identify a lactic acid bacteria (BAL) isolated from native chicken (Gallus gallus domesticus) intestinum (B21) based on 16S rRNA gene sequence and also to confirm primers for PCR-based D-Lactate dehydrogenase (D-LDH) gene isolation. The genomic DNA of B21 was isolated then amplified using 16S rRNA. The PCR product then sequenced and aligned. The sequencing was done in 1st Base Pte. Malaysia. The sequence then aligned using the Basic Local Alignment Search Tool (BLAST) in NCBI to know the similar genome species. Isolation D-LDH gene was done through PCR using 5 primers (idb 0101, idb 1147, idb 0813, idb 1010, and idb 2021) of B21 and also J15 isolate genome. The PCR product were then sequenced and aligned with the D-LDH gene sequences in NCBI. The results of molecular identification based on 16S rRNA markers showed that B21 has 99.45% similarity to Bacillus proteolyticus. Amplification of the LDH gene with idb 1010 primer on J15 isolate yielded 4 fragments (i.e. 650, 1400, 1500, and 1700bp). The results of phylogenetic tests showed that the 1700bp fragment has a high similarity to the D-LDH gene in Myobacterium tuberculosis species with a bootstrap value of 84%. While other size fragments cannot be trusted to have similarity to LDH sequence because they have not reached the minimum bootstrap value that meets the qualifications.
Antibacterial Activity of Green Meniran Extract (Phyllanthus niruri Linn) on The Growth of Salmonella typhimurium Nafisah, Aulia Erta; Octavia, Bernadetta; Lestari, Endang Gati
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.215

Abstract

Salmonella typhimuriumis a bacterium that causes digestive infections, gastroenteritis, and food poisoning, psickcaused byBacterial infection is a health problem in developing countries, including Indonesia. Green meniran (Phyllanthus niruri L.), is a herbaceous plant containing flavonoids, alkaloids, saponins, and tannins which have antibacterial activity which is expected to suppress the development of Salmonella sp. The aim of the study was to determine the ability of green meniran extract as an antibacterial to inhibit the growth of Salmonella typhimurium bacteria. The study used a completely randomized factorial design consisting of two factors, namely extract concentrations of 5%, 10%, 20%, 40%, 80%, equipped with a positive control (chloramphenicol) and negative control (aquades), as well as the age of the inoculum of the bacterial growth phase. Testing the antibacterial activity using the Kirby-Bauer disc diffusion method. The results showed that green meniran extract had antibacterial activity against the growth of Salmonella typhimurium bacteria. Concentrations of 5%, 10%, 20%, 40%, and 80% produced inhibition zone diameters of 6.7 mm, 8.5 mm, 9.9 mm, 12 mm, and 14.6 mm, respectively. The concentration of 80% green meniran extract is effective in inhibiting the growth of Salmonella typhimurium bacteria with an antibacterial effectiveness value of 58.95%.
The Effect of Encyclopedia and Discovery Learning to Improve Learning Motivation and Mastery of Concepts in Student Anggraeni, Neli Dwi Septi; Octavia, Bernadetta
Jurnal Pendidikan Progresif Vol 13, No 3 (2023): Jurnal Pendidikan Progresif
Publisher : FKIP Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The Effect of Encyclopedia and Discovery Learning to Improve Learning Motivation and Mastery of Concepts in Student. Objectives: This study aims to determine effectiveness of encyclopedia structure function of plant tissue and discovery learning to increase learning motivation and mastery of concepts in grade XI MA. Methods: Techniques of data collection in learning motivation with questionnaires and mastery of concepts using tests. Tests and questionnaires were collected twice, namely pretest and posttest. The data obtained carried out prerequisite test analysis with normality and homogeneity test. Furthermore, paired t-test with SPPS V.15. Findings: The results of statistical analysis showed that P-value<0.05 that the encyclopedia structure function of plant tissue and discovery learning was effective in increasing student learning motivation and increasing mastery of concepts in grade XI MA. Conclusion: Effectiveness of encyclopedia structure function of plant tissue and discovery learning can improve learning motivation with effective categories and effectiveness of encyclopedia structure function of plant tissue and discovery learning can improve mastery of concepts with effective categories. Keywords: discovery learning, encyclopedia, learning motivation, mastery of concepts.DOI: http://dx.doi.org/10.23960/jpp.v13.i3.202334
The Effectiviness of Student Teams Achievement Division and Scrambel Combined Model on Collaborative Skills and Conceptual Knowledge Mastery of Class X SMAN 1 Semparuk on Bacteria Alphrazy, Rivaldo; Octavia, Bernadetta
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3544

Abstract

This study aims to analyze whether or not there is a significant difference in collaboration skills and mastery of conceptual knowledge of students who follow the STADBEL combined model learning in the experimental class with students who follow the Make a Match model learning in the control class on the subject of bacteria class X SMAN 1 Semparuk. In this study used non-equivalent control group design. The research sample was students of class X MIPA SMA. Sampling used cluster random sampling technique. The instruments used consisted of conceptual knowledge mastery tests and non-test collaboration skills in the form of questionnaires and self-assessment questionnaires of student responses to learning and student responses to learning models. Learning with the STADBEL combined model can be effective on collaboration skills and mastery of conceptual knowledge of students. A Sig value of 0.027 < 0.05, it is concluded that there is a significant effect of the STADBEL combined model on collaboration skills and mastery of conceptual knowledge of students. These results indicate that the STADBEL combined model effectively improves collaboration skills and mastery of conceptual knowledge of students of SMAN 1 Semparuk class X IPA on the subject of bacteria.
Development of Problem-Based Student Worksheet on Environmental Change Material Astuti, Nadya Welli; Octavia, Bernadetta
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.5678

Abstract

This study aims to determine the feasibility and practicality of problem-based student worksheets on environmental change material. This research is a type of development research (research and development) with research procedures using the 4D Model which consists of the stages of define, design, develop, and disseminate. This research was conducted at SMA Negeri 1 Godean in Class XI semester II. This student worksheet contains environmental problems in Yogyakarta. Based on the results of the study, showed that problem-based student worksheet on environmental change material is suitable for use with a percentage of 71.25% by material experts and 82.1% by media experts. The assessment of the biology teacher as a practitioner expert obtained results with a percentage value of 82.91% with a very practical category to use and an assessment of students with a percentage of 76.82% with a category worth using. It can be concluded that problem-based student worksheets are feasible and practical to use in the implementation of learning biology of environmental change material.
PENGEMBANGAN MODUL ELEKTRONIK BERBASIS SOCIOSCIENTIFIC-ISSUE PADA MATERI VIRUS UNTUK MENINGKATKAN MINAT DAN KEMAMPUAN ARGUMENTASI Sitanggang, Sarah Rapma Kristina; Octavia, Bernadetta; Pratama, Anggi Tias
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.8597

Abstract

This study aims to produce an electronic module based on socioscientific issues that is feasible to improve students' interest and argumentation skills. This research is development research with the ADDIE method (Analysis, Design, Development, Implementation, and Evaluation). The product to be developed is an electronic module based on Socioscientific Issues on virus material. Data collection techniques used are interviews, observations, learning interest questionnaires. The resulting product is then validated by material experts and media experts, and a limited trial is carried out. Based on the results of the development of an electronic module based on socioscientific issues, it is concluded that the electronic module is feasible to be used for biology learning in terms of the assessment of material experts with an average value of 3.11 (very feasible) and the assessment of media experts with an average value of 3.77 (very feasible). And it is effective for increasing students' interest in learning as seen from the n-gains value of 0.55 with a moderate category and to improve students' argumentation skills with an n-gains value of 0.4 with a moderate category.
E-Module Assisted by Augmented Reality with a Discovery Learning Model on Virus Material to Increase Scientific Literacy and Learning Independence for Class X High School Students Saputra, Retno Sandi; Octavia, Bernadetta
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.8795

Abstract

This study aimed to assess the feasibility, practicality, and effectiveness of an e-module assisted by augmented reality with a discovery learning model on virus material to increase scientific literacy and learning independence for class X high school students. The study employed the ADDIE development model by Dick and Carey, employing a quasi-experimental, nonequivalent pretest-posttest control group design. The study involved 60 grade X students from SMA Negeri 1 Pengasih, with class X1 as the control group and X2 as the experimental group selected through cluster random sampling. Various instruments were utilized, including questionnaires from media and material experts, assessments by biology teachers, student responses, as well as tests on learning independence and science literacy. Data analysis encompassed descriptive and inferential statistics. The findings indicated the e-module's suitability, with media experts rating it at 98.37% and material experts at 99%. The practicality evaluation by biology teachers yielded a score of 95.32%, while student responses reached 96.96%. The N-Gain test demonstrated a substantial increase, and the One-Way MANOVA test indicated statistical significance (p <0.05). This e-module has demonstrated its viability, practicality, and efficacy in enhancing scientific literacy and learning independence concerning virus material.
Co-Authors Abd. Rasyid Syamsuri Agus Taftazani Agus Taftazani Agus Taftazani Alphrazy, Rivaldo Anggraeni, Neli Dwi Septi Anna Rakhmawati Aris Bastianudin Astuti Astuti, Nadya Welli Aulia, Fera Ayuningtyas, Dwi Endang Gati Lestari Fellary Pangesti H Yulipriyanto, H Hartanti, Oktavia Dwi Heru Nurcahyo Imam Riadi Imtiyaz, Arifah Nuha Ixora Sartika Mercuriani Kun Sri Budiasih L Sembiring Madhani, Anisa Tiara Mohamad Habibi Nafisah, Aulia Erta Nahar Cahyandaru Nahar Cahyandaru Neli Dwi Septi Anggraeni Nirwana, Putri Nuri Nofia, Fifi Nugraha, Aditya Dirgantara Nur Rizki Putri Ramadhanti Pertiwi, Melania Citra Pramesti, Ariska Hanum Pratama, Anggi Tias Putri Nur Indahsari Rina Rahmawati, Rina Rizka Apriani Putri Saladina, Salma Putri Saputra, Retno Sandi Sari, Herninda Dhama Sitanggang, Sarah Rapma Kristina Siti Nur Azizah Siti Umniyatie Sudarsono Suhandoyo Suhartini Suhartini Suhartini Suhartini Suhartini Tien Aminatun Triwibowo Yuwono