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All Journal JPMS (Jurnal Pendidikan Matematika dan Sains) Jurnal Penelitian Saintek Jurnal Penelitian Humaniora INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni Ganendra: Majalah IPTEK Nuklir Jurnal Sains Dasar Biotropika Indonesian Journal of Biotechnology Scientiae Educatia: Jurnal Pendidikan Sains Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Journal of Education Technology Jurnal Abdi Insani Jurnal Manajemen Pendidikan dan Ilmu Sosial (JMPIS) Borobudur Jurnal Natural Kingdom (The Journal of Biological Studies) IJOBI Jurnal Pendidikan Progresif
Bernadetta Octavia
Department Of Biology Education, Faculty Of Mathematics And Natural Sciences, Universitas Negeri Yogyakarta, Yogyakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Neuroscience Physics Public Health Social Sciences Other

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Keanekaragaman Cacing Tanah Pada Lahan Sawah Tercemar Residu Pestisida Nur Rizki Putri Ramadhanti; Tien Aminatun; Anna Rakhmawati; Bernadetta Octavia; Suhartini Suhartini
Jurnal Sains Dasar Vol 12, No 1 (2023): April 2023
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v12i1.58953

Abstract

Penelitian ini bertujuan mengetahui kondisi fisikokimia dan keanekaragaman cacing tanah pada lahan sawah tercemar residu pestisida di Wonokerto, Turi, Sleman, Yogyakarta. Penelitian ini merupakan jenis penelitian observasi yang mendeskripsikan keanekaragaman cacing tanah di lahan tercemar residu pestisida terutama golongan organofosfat. Lahan penelitian meliputi lahan A dengan umur penggunaan pestisida organofosfat lebih dari 1 tahun dan lahan B berumur kurang dari 1 tahun. Pengambilan sampel menggunakan teknik handsorting pada petak 25x25x25cm. Sampel diidentifikasi di Laboratorium Lingkungan FMIPA UNY. Analisis data secara deskriptif berdasarkan hasil indeks keanekaragaman Shannon–Wienner. Hasil uji kandungan pestisida golongan organofosfat lahan A dan lahan B adalah tidak terdeteksi. Namun berdasarkan paparan petani, lahan A dan lahan B menggunakan pestisida golongan organofosfat dengan bahan aktif profenofos selama lebih dari 1 tahun. Untuk itu, ke dua lahan diperkirakan tercemar pestisida golongan organofosfat. Hasil uji fisikokimia tanah lahan A bertekstur geluh pasiran dengan kandungan bahan organik dan C organik lebih rendah serta pH, KTK, N total, P tersedia, dan K tersedia lebih tinggi dibanding lahan B bertekstur pasir geluhan. Tingkat keanekaragaman cacing tanah adalah rendah pada ke dua lahan dengan indeks keanekaragaman tertinggi 0,617 pada lahan B berumur kurang dari 1 tahun penggunaan pestisida golongan organofosfat.Kata kunci: keanekaragaman, cacing tanah, cemaran, residu pestisida.This research aims to discover physicochemical conditions of soil and diversity of earthworms in fields contaminated pesticide residues in Wonokerto, Turi, Sleman, Yogyakarta. This research is observational research describes earthworms’ diversity in fields contaminated pesticide residues, especially organophosphate. Research area includes field A, with exposure to organophosphate pesticides for more than 1 year, and field B less than 1 year. The handsorting was employed as a sampling technique in plots of 25x25x25cm. Samples were identified at Environmental Laboratory FMIPA UNY. Data analysis was conducted descriptively based on results Shannon–Wienner diversity index. The test results show pesticide organophosphate content in two fields was undetected. However, based on the explanation from farmers in field A and B, used organophosphate pesticides with active ingredient profenophos more than 1 year. It is estimated, both fields will remain contaminated organophosphate pesticides. The soil physicochemical tests indicate field A, a sandy loam texture, had lower organic matter content and organic C with higher pH, CEC, total N, available P, and available K compared to field B with a loamy sand texture. Diversity level of earthworms is low in both fields with highest index being 0,617 in field B, less than 1 year using organophosphate pesticides.Keywords: diversity, earthworms, contamination, pesticide residue.
Assessing soil bacterial community response to organophosphate pesticides in agricultural field of Yogyakarta, Java, Indonesia ANNA RAKHMAWATI; BERNADETTA OCTAVIA; SUHARTINI SUHARTINI; TIEN AMINATUN
Jurnal Natural Volume 23 Number 3, October 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v23i3.31263

Abstract

Soil contamination by pesticides is one of the world’s most pressing environmental issues. The widespread use of Organophosphate pesticides (OPPs) in agriculture has led to biological diversity changes. The indigenous bacterial community played significant roles in the remediation of soil contaminated with OPPs. This study examines the overall bacterial community composition of three agricultural fields in Yogyakarta, Java, Indonesia, that were exposed to OPPs. The agricultural field was divided into zones near the beach, residential, and mountainous. Sequencing 16S rRNA amplicon fragments used to analyze the soil bacterial community. It was discovered that Proteobacteria, Actinobacteria, and Firmicutes comprised the majority of the bacterial community. In addition, the samples contain a high relative abundance of Bacillus, Bradyrhizobium, Chryseobacterium, Cystobacter, Microvirga, and Burkholderia. The high alpha diversity indexes suggest that the agricultural soil microbiome provides important ecological services and may harbor a wide variety of bacteria and genes with biotechnological applications. The physicochemical soil characteristics are also correlated with the bacterial community structure. The findings can be used to develop bioremediation strategies that employ native microbes to clean and restore agricultural soil contaminated with OPPs.
IDENTIFIKASI BAKTERI PADA BINTIL AKAR AKTIF DAN TIDAK AKTIF SERTA RHIZOSFER TANAMAN KACANG PANJANG Hartanti, Oktavia Dwi; Octavia, Bernadetta
Kingdom: The Journal of Biological Studies Vol 9, No 2 (2023): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v9i2.18627

Abstract

Rendahnya kandungan N dalam tanah dapat ditangani dengan memanfaatkan mikroba tanah penambat N yang memiliki kemampuan menambat nitrogen di udara dan berguna sebagai penyedia unsur hara dalam tanah sehingga nantinya tersedia untuk tanaman. Tujuan penelitian ini adalah untuk mengetahui hasil isolasi bakteri, karakteristik isolat bakteri, dan perbedaan keanekaragaman bakteri dari bintil akar aktif dan tidak aktif serta rhizosfer tanaman kacang panjang. Jenis penelitian yang dilakukan yaitu deskriptif eksploratif. Sampel bintil akar dan rhizosfer diambil di sawah Dusun Blawong II. Dan penelitian dilakukan di Laboratorium Mikrobiologi FMIPA UNY. Karakterisasi fenotipik dilakukan dengan uji morfologi koloni, morfologi sel, dan uji fisiologis (biokimiawi). Identifikasi bakteri dilakukan dengan metode profile matching, untuk mengetahui indeks similaritasnya dengan genera bakteri acuan. Dari hasil penelitian diperoleh total 14 isolat murni yang memiliki karakteristik yang beragam. Dari hasil identifikasi terdapat 6 genera bakteri yang diperoleh yaitu genera Neisseria pada sampel bintil akar tidak aktif dan rhizosfer, Rhizobium pada sampel rhizosfer, Azomonas pada sampel bintil akar aktif dan rhizosfer, Mesorhizobium pada sampel bintil akar tidak aktif, Streptococcus pada sampel bintil akar tidak aktif dan rhizosfer, serta Staphylococcus pada sampel bintil akar aktif.
IDENTIFIKASI BAKTERI PADA BINTIL AKAR AKTIF DAN TIDAK AKTIF SERTA RHIZOSFER KACANG TANAH Imtiyaz, Arifah Nuha; Octavia, Bernadetta
Kingdom: The Journal of Biological Studies Vol 9, No 1 (2023): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v9i1.18626

Abstract

Kemiskinan unsur hara N dalam tanah dapat diatasi dengan memanfaatkan kelompok mikroba tanah yang dapat berguna sebagai penyedia unsur hara pada tanah yang nantinya tersedia untuk tanaman. Tujuan penelitian ini adalah untuk mengetahui hasil isolasi bakteri, karakteristik fenotipik bakteri, serta perbedaan keanekaragaman bakteri dari bintil akar aktif dan tidak aktif serta rhizosfer tanaman kacang tanah (Arachis hypogaea L.). Penelitian ini di Laboratorium Mikrobiologi FMIPA UNY. Sampel bintil akar dan rhizosfer diambil di sawah, di Kabuaten Magelang, Jawa Tengah. Pengujian Karakteristik fenotipik meliputi karakterisasi morfologi koloni, morfologi sel, dan uji fisiologis (biokimia). Identifikasi bakteri dilakukan dengan metode profile matching, untuk mengetahui indeks similaritasnya dengan genera bakteri acuan. Diperoleh total 15 isolat yang merupakan bakteri gram positif dan negatif, berbetuk coccus maupun bacil, dengan konfigurasi round, margin smooth, elevasi raised, berwarna putih hingga putih kekuningan, dengan hasil uji fisiologis (biokimia) yang beragam. Setelah dilakukan proses identifikasi, diperoleh 2 genera bakteri yang ada pada bintil akar aktif yaitu Azomonas dan Staphylococcus, 3 genera bakteri yang ada pada bintil akar tidak aktif yaitu Mesorhizobium, Azomonas, dan Bacillus, serta 3 genera bakteri yang ada pada rhizosfer tanaman kacang tanah yaitu Rhizobium, Pseudomonas, dan Neisseria.
INHIBITION OF MANGKOKAN LEAF (Nothopanax scutellarium Merr.) ETHANOL EXTRACT ON THE GROWTH OF Salmonella typhimurium BACTERIA Sari, Herninda Dhama; Octavia, Bernadetta
Indonesian Journal of Bioscience (IJOBI) Vol. 2 No. 1 (2024): Indonesian Journal of Bioscience (IJOBI)
Publisher : Department of Biology Education FMIPA Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v2i1.747

Abstract

Typhoid fever is an infectious disease of the digestive tract caused by the bacterium Salmonella typhimurium. Mangkukan leaf (Nothopanax scutellarin Merr.) can be an alternative treatment because it contains flavonoids, saponins, tannins, and alkaloids. This study aimed to determine the ability of Mangkukan leaf extract as an antibacterial in inhibiting the growth of S. Typhimurium bacteria. This study used a factorial completely randomized design consisting of two factors, namely the concentration of the extract and the age of the inoculum with 4 repetitions. The concentration of the extract used was 5%, 10%, 20%, 40%, and 80%, equipped with the positive control (chloramphenicol), and negative control (aqua dest), while the age of the inoculum was based on the growth phase of the test bacteria, namely 3 hours, 6 hours, 18 hours. hours, and 24 hours. Antibacterial activity testing was carried out using the Disc Diffusion method (Kirby-Bauer Test). The test concentrations of 5%, 10%, 20%, 40%, and 80% test concentrations resulted in inhibition zone diameters of 3.41 mm, 5.4 mm, 6.04 mm, 7.43 mm, and 9.48 mm, respectively. The 80% concentration of ethanol extract from the kukukan leaf was effective in inhibiting the growth of S. typhimurium bacteria with an antibacterial effectiveness value of 60.81%.
Visualizing the Human Reproductive System Material Through Web-based E-module Rahmawati, Rina; Octavia, Bernadetta; Ayuningtyas, Dwi; Nugraha, Aditya Dirgantara
Journal of Education Technology Vol. 8 No. 3 (2024): August
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jet.v8i3.53213

Abstract

The 21st century is the digital age, so learning is expected to take advantage of technology. Teachers have not utilized technology as much as possible so learning in the classroom is not optimal. This study aims to develop a web-based e-module teaching material on the human reproductive system for high school/MA class XI students. The research and development model uses ADDIE. The research subjects consisted of 1 media expert, one material expert, two biology teachers, and 30 students. Data collection methods consist of observation, interviews, and questionnaires. Data analysis used descriptive qualitative and quantitative descriptive. The results showed that the assessment score of media experts was 3.82 (Very Valid); the score of material experts was 3.50 (Valid); the score of the biology teacher was 3.91 (Very Valid), and a student score of 3.46 (Valid). Therefore, it can conclude that the web-based e-module on the human reproductive system material is suitable for biology learning. Web-based e-modules can help class XI students to study independently anytime and anywhere and to understand material on the human reproductive system.
Pelatihan Budidaya Maggot untuk Meningkatkan Ketrampilan Masyarakat dalam Pengolahan Limbah Organik Rumah Tangga dan Menciptakan Peluang Usaha di Kelurahan Umbulmartani, Ngemplak, Sleman Octavia, Bernadetta; Rakhmawati, Anna; Aminatun, Tien; Suhartini, Suhartini; Aulia, Fera
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol. 9 No. 1 (2025): Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jpmmp.v9i1.82730

Abstract

The accumulation of rubbish at temporary waste storage locations has become a common concern for us. The organic waste in the rubbish pile usually causes a very strong pungent odor. In fact, if organic waste is managed from every house/location that produces it, it will very significantly reduce both the volume of rubbish piles and the pungent stench. Therefore, it is necessary to instill awareness in every member of society about the importance of managing waste and specifically organic waste which can be degraded by various types of organisms. One of the organisms that can be used to process this organic waste is maggot, which is the larva of the black soldier fly (BSF). Apart from being an organic waste processing agent, maggots can also be used as a feed supplement for chickens and fish. In this way, maggot cultivation can have a dual function for community members who are motivated to manage organic waste independently. Through Lecturer Activities Off Campus (DLK) activities this year, the community in Umbulmartani Village, Ngemplak District, Sleman was given training on Maggot Cultivation to be used as an organic waste processor on a household scale and can also be used to improve the family economy if maggot cultivation is carried out managed seriously. The final activity is evaluation. The program results show the success of the process as indicated by: a. Attendance of participants reaches 100%, b. Increasing knowledge and skills in cultivating maggots for processing family organic waste, c. The results of the participant satisfaction questionnaire showed that participant satisfaction in all aspects was good and very good.
PEMASYARAKATAN TEKNIK PEMBUATAN KOMPOS DENGAN MENGGUNAKAN CACING TANAH (VERMICOMPOSTING) SEBAGAJ UPAYA UNTUK PENANGANAN LIMBAH ORGANIS DAN PENINGKATAN PENDAPATAN PETANI DI PEDESAAN Yulipriyanto, H; Sudarsono, Sudarsono; Suhandoyo, Suhandoyo; Suhartini, Suhartini; Octavia, Bernadetta
INOTEKS: Jurnal Inovasi Ilmu Pengetahuan,Teknologi, dan Seni Vol 1, No 1 (1999)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ino.v1i1.5102

Abstract

Kegiatan  ini bertujuan  untuk  memasyarakatkan  teknik  pembuatan  kompos  dengan  menggunakan  cacing  tanah(vermicomposting) sebagai salah satu alternatifdalam  penanganan limbah organik bagi masyarakat petani pedesaan.Khalayak sasaran kegiatan ini adalah petani pedesaan di desa Sardonoharjo, kecamatan Ngaglik, dan kelompok kandang "Andhini Sembada" desa Kepuh Harjo, kecamatan Cangkringan semuanya terdapat di wilayah kebupaten Sleman Daerah Istimewa Yogyakarta.   Kegiatan diselenggarakan pada bulan Agustus 1996 hingga Januari 1997. Kegiatan ini terlaksana berkat adanya kerja sama antara petani masyarakat di kedua desa pembangunan stasiun pembuatan kompos dengan agen perombak cacing tanah, menyediakan bahan-bahan yang dibutuhkan serta tenaga kerja, sedang perguruan tinggi berlaku sebagai nara sumber. Dalam kegiatan ini digunakan kotoran ternak (sapi),  sampah kandang, jerami kering.Hasilnya menunjukkan bahwa masyarakat desa memperoleh pengalaman baru perihal cacing tanah yang mempunyai kemampuan merombak Iimbah organik. Setelah memperoleh pengetahuan tentang caeing tanah dan cara-cara pengomposan dengan cacing tanah, maka setelah diadakan pembangunan stasiun percobaan kompos, masyarakat menganggap bahwa cara­ cara yang diterapkan sangat mudah dan tidak memerlukan teknologi tinggi. Dengan kata lain respon masyarakat cukup positif. Apalagi di sekitar lokasi percobaan dekat kawasan wisata baru lapangan golfMerapi, yang membutuhkan keteduhan untuk ditanami berbagai pohon yang tentunya membutuhkan pupuk organik kompos.
Tempoyak as a Biotechnology Learning Media: Innovation of Ethnoscience-Based PjBL E-module to Improve Science Literacy and Creativity of Phase E Students Herlinawati, Nabila; Octavia, Bernadetta
Scientiae Educatia: Jurnal Pendidikan Sains Vol 14, No 1 (2025): June 2025
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v14i2.20010

Abstract

This research is motivated by the limitations of teaching materials that are less interactive and innovative and the low scientific literacy and creativity of students. The purpose of this study is to develop an ethnoscience-based Project-Based Learning (PjBL) e-module in making Tempoyak as a biotechnology teaching material to improve scientific literacy and creativity of Phase E students. The method used is Research and Development (R&D) with a 4D development model (Define, Design, Develop, and Disseminate). The research sample consisted of two classes, namely the experimental class using the ethnoscience-based PjBL e-module and the control class using conventional teaching materials. The effectiveness of the e-module was measured by comparing the results of the pretest and posttest, as well as the creativity questionnaire. The results showed that the developed e-module was effective in improving the scientific literacy and creativity of students. The average posttest score of the experimental class increased significantly compared to the control class. Thus, this e-module can be an alternative innovative teaching material that integrates science with local culture, which can improve scientific literacy and creativity.
Identification and genetic study of lactic acid bacteria from intestine of domestic chickens (Gallus gallus domesticus) Ixora Sartika Mercuriani; Heru Nurcahyo; Bernadetta Octavia; Astuti Astuti; Adinda Yuslia Rukmanandita; Fita Nilasari
Indonesian Journal of Biotechnology Vol 30, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.85963

Abstract

The gastrointestinal tract is one of the habitats of lactic acid bacteria (LAB). This research aims to identify the LAB isolated from the intestines of domestic chickens (Gallus gallus domesticus) based on phenotypic and genotypic characteristics. The LAB was cultivated on enrichment media MRSA + CaCO3 1% and a selection of isolates based on the halo zone. The phenotypic character identification using morphological, physiological and biochemical tests was analyzed by similarity simple matching (SSM) and a dendrogram (MVSP 3.1). Genotypic identification was made by 16S rRNA gene sequences with PCR methods, sequencing and analysis using BLAST at www.ncbi.nlm.nih.gov. The results show that 87 isolates were isolated based on the halo zone. Phenotypic tests were performed on eight isolates (J6, J15, J18, J28, B11, B24, B25 and B26). The results show that four isolates have similarities with the genus Bacillus, two isolates with the genus Lactobacillus, and two with the genus Streptococcus. The identification based on the 16S rRNA gene of four selected isolates (J15, J28, B24 and B26) showed that J15 was identified as Bacillus cereus (98.56% similarity); J28 as Lactobacillus johnsonii (99.67%); B24 as Bacillus cereus (98.30%), and B26 as Streptococcus pluranimalium (96.68%).
Co-Authors Abd. Rasyid Syamsuri Adinda Yuslia Rukmanandita Agus Taftazani Agus Taftazani Agus Taftazani Alphrazy, Rivaldo Aminatun, Tien Anggraeni, Neli Dwi Septi Anna Rakhmawati Aris Bastianudin Astuti Astuti Astuti Astuti, Nadya Welli Aulia, Fera Ayuningtyas, Dwi Endang Gati Lestari Fellary Pangesti Fita Nilasari H Yulipriyanto, H Hafizh Adyatma Hartanti, Oktavia Dwi Herlinawati, Nabila Heru Nurcahyo Heru Nurcahyo Imam Riadi Imtiyaz, Arifah Nuha Irenne Odly Kathleen Pasassung Isti'anah, Rayi Kuni Ixora Sartika Mercuriani Kun Sri Budiasih L Sembiring Madhani, Anisa Tiara Mohamad Habibi Nafisah, Aulia Erta Nahar Cahyandaru Nahar Cahyandaru Neli Dwi Septi Anggraeni Nofia, Fifi Nugraha, Aditya Dirgantara Nur Rizki Putri Ramadhanti Nuradiah, Mawaddah Fatin Pertiwi, Melania Citra Pramesti, Ariska Hanum Pratama, Anggi Tias Pujiati TM, Indah Putri Nur Indahsari Putri Nuri Nirwana Raphasya, Fhadinda Hizza Rina Rahmawati, Rina Rizka Apriani Putri Saladina, Salma Putri Saputra, Retno Sandi Sari, Herninda Dhama Sitanggang, Sarah Rapma Kristina Siti Nur Azizah Siti Umniyatie Sudarsono Suhandoyo Suhartini Suhartini Suhartini Suhartini Suhartini Triwibowo Yuwono