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THE EFFECT OF DIFFERENT CONCENTRATION OF CRUDE BROMELAIN ENZYM TO CATFISH (Clarias gariepinus) SAUCE Kartika Dewi; Rahman Karnila; Suardi Loekman
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was intended to determine the effect of crude bromelain enzym withdifferent concentration to catfish (Clarias gariepinus) sauce. The fuction of crude bromelainenzym as catalysator which helping of hydrolisis in catfish (Clarias gariepinus) saucefermentation. The crude bromelin enzym extracted from fruit of pineapple. Crude bromelainenzym concentration which used for this research was 3% (B3), 6% (B6), and 9% (B9). The bestconcentration of crude bromelain enzym for catfish sauce quality was B9, because producedhighest hydrolisat volume and protein content which was 99.67 ml, 77.86% (bk) respectively.Total oganoleptic value which made with 9% concentration of crude enzym bromelain the wasmost favorable by consumer acceptance with 5.21 colour value, 4.36 taste value, 8.07 texturevalue and 5.91 odor value.Keywords : Fermentation , catfish ( Clarias gariepinus ), Crude bromelain enzym,fish sauce
COLLAGEN EXTRACTION OF PATIN FISH SKIN (Pangasius sp.) WITH DIFFERENT PAPAIN ENZYMES CONCENTRATION Bunga M.W Bunga M.W; Rahman Karnila; Mery Sukmiwati
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 8, No 1 (2021): Edisi 1 Januari s/d Juni 2021
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract This study aims to determine the effect of different papain enzyme concentrations on collagen extraction from the skin of catfish (Pangasius sp.). The method used was an experimental method with completely randomized concoctions (CRD). The treatments given consisted of E0 (Control), E1 (5% papain enzyme), E2 (10% papain enzyme), E3 (15% papain enzyme). This research consisted of 3 stages, namely 1) preparation of raw catfish, 2) deproteination of catfish skin, 3) hydrolysis of raw catfish with acetic acid.The parameters tested were collagen yield, proximate analysis (moisture content, ash content, and protein content), pH, and functional group (FTIR). The results showed that the quality of the collagen obtained increased along with the increase in the concentration of the enzymes used. This is presumably because the addition of the enzyme concentration in the collagen extraction process will increase the yield produced because the enzyme breaks the cross-linking of the collagen telopeptide and dissolves it. Based on this, the use of the optimum concentration was obtained at a concentration of 15% (E2) with a protein content of 71,60%, 14.17%water, and4.21% ash Keywords:Collagen, Catfish, Extraction, Papain Enzymes.
THE EFFECT OF THE HYDROCHLORIC ACID CONCENTRATION TO THE CHARACTERISTICS OF BLACK SEA CUCUMBER (Holothuria edulis) CHITIN Vran Orlando Josua; Edison '; Rahman Karnila
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research aims to determine the physico-chemical characteristics of black sea cucumber ( Holothuria edulis) chitin, get the best hydrochloric acid concentration in demineralization process from crude chitin to chitin, and to determine the highest deacetylation degree of black sea cucumber chitin. This research was conducted in two phases. The first was preparation and the second was drying, sieving, and manufacturing the chitin. The result showed that the different concentration of HCl did not effect significantly to the water content and ash content of black sea cucumber chitin. Meanwhile, the highest deacetylation degree was obtained by the using of HCl at the consentration of 1 N, followed by the concentration 0,5 N then 1,5 N.Keywords : ash content, black sea cucumber, deacetylation degree, water content.
CHARACTERISTICS OF ORGANOLEPTIC QUALITY AND pHBAUNG (Mystus nemurus) WITH SOAKING IN THE CHITOSAN SOLUTION Jhosua Fransiskus; Rahman Karnila; Andarini Diharmi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractThis study was aimed to determine the organoleptic quality and pH of fresh baung (Mystus nemurus) soaked with chitosan solution with different concentrations. This research used the experimental method, by immersing the fish with chitosan solution with a concentration of 0% (without chitosan), 1, 2, and 3% and soaking time 0, 4, 8 and 12, for 16 hours. The analysis parameters were consisted of organoleptics including eye appearance, gills, mucus, meat (color and appearance), odor, texture, and pH test. The results were showed that immersion of baung in high concentration of chitosan can maintain freshness of baung fish. Soaking fish in 3% chitosan solution is able to maintain freshness with freshness for 12 hours aimed at the value of the eye value of 6.79 (sunken eyes, pupils turn white, cloudy corneas); gills with a value of 7.05 (pale red color, without mucus); odor 6.87 (Neutral). Immersion in chitosan solution for 16 hours produced atexture of baung fish 7.13 (rather soft, less elastic when pressed with a finger, difficult to tear meat from the spine); meat with a value of 7.12 (less brilliant, specific type, no milking along the spine, abdominal wall of whole meat);mucus with a value of 7.05 (The mucus layer starts to become slightly cloudy, the color is rather white, less transparent); and pH 6. The best treatment for this is immersion of baung fish with a concentration of chitosan 3%. Keywords: eye,freshness, solution concentration, texture,
DETERIOTATION IN FISH QUALITY SNAKEHEAD (Channa striata) WITH DIFFERENT LETHAL TECHNIQUE AT ROOM TEMPERATURE 28˚C Dewi Trisnawati; Rahman Karnila; N. Ira Sari
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 7, No 1 (2020): Edisi 1 Januari - Juni 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThis study was aimed to determine determine the lethal techniques to maintain the quality of the freshness of snakehead fish for 12 hours. This research was an experiment with a non factorial complete random design. The treatment was used a lethal fish technique consisting of piercing parts of the brain / Medulla oblongata (A1), cold temperatures (A2), and beating (A3). Observations after the fish is turned off by turning off different techniques performed at intervals of 0, 4, 8, and 12 hours on the sensory quality, chemical and microbiological. The analysis parameters were consisted of organoleptic eyes, gills, and odors), chemistry (TVB, glycogen), and total microbes. The results showed that snakehead fish with deadly technique by being stabbed, cold temperature, was produced organoleptic quality (eyes, gills, and odors) from 0-8 hours the quality can still be maintained freshness.  The Treatment of A1, on the hour to 12 organoleptic (eyes, gills, and smell) still worth resulting average value 6:00, except treatment A2, and A3, are not worthy for consumption (spoiled). TVB analysis results, during the observation of 0- 12 hours of snakehead fish with different way of death experienced an increase at 8 hours still according of quality standards, the 12th hour TVB snakehead fish value > 25 (spoiled). The lowest glycogen levels in treatment A3, at 4-12 hours instead of A2 and A3. Microbiologically, the treatment of A3 treatment has the highest total microbial value. The best lethal technique is by getting a fish stabbed in a part of the brain that can maintain the freshness of snakehead fish for 12 hours. Snakehead are turned off by plugging in the brain, organoleptic (eyes, gills and smell), chemistry (TVB and glycogen), and the total microbiological according to SNI quality standards for fresh fish.Keywords: glycogen, lethal techniques of fish, organoleptic, TPC, TVB.1)Student 
DEMINERALIZATION OF CHITIN FROM VANNAME (Liptonaeus vannamei) SHRIMP SHELLWASTE USING CITRIC ACID AT DIFFERENT pH Windi Ayu Maltadevi; Rahman Karnila; Edison Edison
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol. 9 No. 1 (2022) Edisi 1 Januari s/d Juni 2022
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT               Vanname shrimp (Liptonaeus vannamei) is widely consumed by the community, on the other hand, the skin waste is underutilized. Shrimp shell has a potential use as a chitin product. Chitin can be isolated from shrimp shell by demineralization and then deproteination their shells. The demineralization process was carried out using citric acid. This study aimed to determine the effect of different pH on the use of citric acid in the manufacture of chitin. The research method used was experimental composed as a non-factorial completely randomized design (CRD) with 3 treatment levels, namely K1(pH 4), K2(pH 5) and K3 (pH 6). The best characteristics of vannamei shrimp waste chitin with citric acid at pH 4 indicated by the highest yield of 48.82%, water content 2.18%, ash content 3.30%, and the degree of deacetylation of 40.3%. Keywords:Chitin, Citric acid, pH, Vanname shrimp
CHEMICAL ANALYSIS AND CHARACTERISTICS OF VANNAME SHRIMP (Litopenaeus Vanname) CARAPACE FLOUR Mhd Asrel Ahmad; Rahman Karnila; Edison Edison
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 7, No 2 (2020): 2 Juli - Desember 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT                 Vanname shrimp is one type of shrimp that is widely consumed by the public because it has high nutritional value. This study aims to determine the proportion and proximate chemical composition of vanname shrimp carapace flour. This research method consists of two stages, namely: 1) Preparation of making vanname shrimp carapace flour, 2) Proximate analysis of vanname shrimp carapace flour. The parameters measured in the first stage are the calculation of the proportion value of the body part of the vanname shrimp and the second stage is the calculation of water, ash, protein, fat and carbohydrates content. The results of this study were the calculation of the proportion value of vanname shrimp body parts as follows: 75.10% meat, 15.23% head and 10.75% skin. Proximate chemical composition of vanname shrimp carapace flour is as follows: water content 10.46% (WW), ash content 32.32% (DW), protein content 38.38% (DW), fat content 1.95% (DW) and carbohydrates content 2.75% (by different).Keywords: vanname shrimp, proportion, carapace flour, proximate.
EFFECT OF DIFFERENT CONCENTRATION OF PAPAIN ENZYME TOWARDTOTAL AMINO ACID OF EEL (Monopterus albus) PROTEIN HYDROLYSATE Ayu Agustina; Rahman Karnila; Mirna Ilza
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT                This research was aimed to determine the proximate chemical content of eel (Monopterus albus) meat, obtain the eel protein hydrolysate from different concentration of papain enzymes, and determine the total amino acid of eel protein hydrolysate. The method used was an experimental method with a Completely Randomized Design (CRD). The treatments used was various concentrations of papain enzymes P1(3%), P2 (5%), P3(7%). Parameters observed were proximate analysis (moisture, ash, protein, fat, and carbohydrate content) of eel protein hydrolysate, total amino acid analysis and yield. The results showed that the proximate content of eel meat was 81.28% moisture content, 1.76% ash content, 14.54% protein content, 1.28% fat content, and 1.14% carbohydrate (by difference). Total amino acids analysis of P1, P2, and P3 treatments were 14.03%, 14.86%, and 15.66%, respectively.The different concentration of papain enzymes had a very significant effect on yield, whereas the yields of P1, P2, and P3 treatments were 33.05%, 35.25%, and 37.15%, respectively. Keywords: Fish protein hydrolysate, eel, papain enzyme
STUDY OF CONSUMER ACCEPTANCE AGAINST CATFISH (Pangasiusdjambal)MEATBALLS WITH SUBSTITUTION OF YELLOW SWEET POTATO Rokhimin Rokhimin; Rahman Karnila; Suparmi Suparmi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRAK  This research was aimed to determine the best amount of yellow sweet potato flour on processing of catfish (Pangasiusdjambal)meatballs. The method used in this study was the experimental method that was processing the catfish meatballs substitued with yellow sweetpotato flour. The design used was a Completely Randomized Design (CRD) non factorial with 4 treatment levels and 3 replicates. The treatment was the different concentration of yellow sweet potato with 4 treatment levels: Bo (without addition of yellow sweet potato flour/control), B1(25 g of yellow sweet potato flour), B2 (35 g of yellow sweet potato flour), and B3(50 g of yellow sweet potato flour) with 3 replicates. The parameter used in this research was organoleptic (appearance, texture, aroma, and taste) and chemical analysis (moisture, fat,protein, and ash content). Based on the result showed that B1 treatment was most preferable by consumer acceptance(98,43%) which characteristics of appearance was brown grayish colour, specific aroma of catfish meatballs, soft and chewy texture, spices flavor. The proximat analysis of moisture, fat, protein, and ash content was 73.07%, 1.59%, 14.91%, and 1.66%, respectively. Keywords: catfish meatballs, consumer acceptance, substitution
ANALYSIS OF MUDSKIPPER (Periophthalmodonschlosseri) CHEMICAL CONTENT IN DIFFERENT STEAMING TEMPERATURES Esfi Girsang; Edison Edison; Rahman Karnila
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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 ABSTRACT The objective of the study was to examine the effect of varied steaming temperatures on the chemical composition of the fish flour (Periophthalmodonschlosseri). The method used was experimental and composed as Completely Randomized Design (CRD) by conducting the treatment of steaming the fish at different temperatures (60, 70, and 80 oC). The parameters assessed were the rendemen and the psycho-chemical characteristic of the fish flour. The results showed that nutrient proximate composition in fresh fish meat the dry based content of water 79.13% protein 92.83%, ash 4.54%, fat 1.13%, and carbohydrates1.50%. Steaming temperature 60oC was showing the best treatment, indicatedby yielded rendement 17.34%, the content of water 9.97%, protein 82.93%, fat 1.34% and ash 318%. The treatment of different steaming temperatures conducted was not affecting significantly to the physical appearance of fish flour, indicated by the similar aroma and texture characterized as brownish yellow color. The higher the temperature used, the darker the flour yielded color. Keywords: Mudskipper, flour, temperature, steaming
Co-Authors ', Dahlia ', Desmelati Afriadi Sinaga Ainiwati Ainiwati Anak Agung Istri Sri Wiadnyani Anda rini Diharmi Andarini Diharmi Andri Hendrizal Andriani, Andarini Anggito Naibaho Angraika Dirta Ani Karmila Anindinda Prameswari Hastiza Anisa Anastasia Yunus Annisa Sharah Antonius Zulqaiman Aras Mulyadi Asfi, Widya Mustika Auladi, Asyik Ayu Agustina Bayhakki Bayhakki Bella Andita Anggreini Bintal Amin Bintang, M. Irwan Sapta Purna Budijono, Budijono Bunga M.W Bunga M.W Bustari Hasan Cindytia Prastari Dahlia Dahlia Daviny, Nindy Deddy Purnama Defri Ilham Desmelati ' Desmelati Desmelati Desmelati Desmelati Desnizarianti ' Devi Meri Yati Dewi Trisnawati Dewita Dewita Dewita Dewita Dhea Oktama Rivaldo Dian Iriani Dian Maulidawati Dini Fitri Edis Edis Edison ' Edison Edison Edison Edison Edison Edison Edison Edison Efriyeldi, Efriyeldi Ega Aryanda Putra Eko Prianto, Eko Elda Nazriati Endang Sulistya Ningsih, Endang Sulistya Eni Sumiarsih Esfi Girsang Esy Maryanti Fadhlami, Putri Insani Fajar Syukron Faradini Faradini Fatma Eka Putri Febrigusni, Audia Febrina Tumangger Feliatra Ganda Aliem Pakarsya Lubis Ghazali, Tengku Muhammad Gianthi, Fiskia Gita Amelia Gusti Putra Yunius Henni Syawal Heriansyah, Ivan Heriansyah, Ivan Hilyati Fadhlah Ika Darmila Siregar Indah Khairunnisa Indra Muhammad Ridwan Intan Elisa Irvan Irvan Ismulyati Ismulyati Ismulyati, Ismulyati Jamal Basmal Jayanti, Tinneke Dwi Jeremy Chrisdwi Putra Hutajulu Jhosua Fransiskus Kartika Dewi Kembaren, Bella Billiant J Ananta Lesi Kurnia Putri M Irwan Sapta Purna Bintang M Lamudin Noor M. Alhadid M. Alhadid MADE ASTAWAN Mery Sukmiwati Mery Sukmiwati Mhd Asrel Ahmad Mirna Ilza Mirna Ilza Mirna Liza Moh Wahbi Mubarak Mubarak Muhammad Raihan Munisatul Haq Muslihul Makrif N. Ira Sari Nadia Adilla Nadia Mahardika Nasib Naibaho, Nasib Nindy Daviny Nintha Pratiwi Nittaya Chaiyanate, Nittaya Nofrizal Nofrizal Nofrizal Nofrizal Nur Azima Nur Wahid, Nur Oktama Rivaldo, Dhea Putra, Harifa Syah Putra, Muhammad Ridho Putri, Dea Arsifah Putri, Fatma Eka Rachmiwati Yusuf Radian Saputra Ramadhani, Nuzul Ria Rana Salma Ridwan Manda Putra Rifati Hanifa Rivaldo, Dhea Oktama Riwanda, Wahyu Rokhimin Rokhimin Rommy Novrihansa Ronal Kurniawan Rony Setyawan Rovilah, Kanza Roy Susanto Rozi Rudy R Nitibaskara Sam Herodian Saparudi Saparudi Sayyidina Ahmad Zhariif Selamat ariyadin Septia Murni, Septia Septin Alvin Mendrofa Shabrina Nur Fakhriah Sholihah, Aulia Putri Sidauruk, Shanty Wisuda Siregar, Riri Andriani Siti Humairah Suardi Loekman Sukarno Sukarno Sukendi Sukendi Sukirno Mus Sulistiana Pramita Nosa Sumarto Sumarto Sumarto Sumarto Sumarto, Sumarto Suparmi ' Suparmi Suparmi Suyanto Suyanto Syafruddin Nasution Syahrul Syahrul Syamsiar, Syamsiar Tedy Kamal Sumannag Tengku Muhammad Ghazali Tengku Muhammmad Ghazali, Tengku Muhammmad Tengku Nurhidayah Teresia Br Ginting Thamrin Thamrin Thamrin Thamrin Thamrin Thamrin Tia Novita Laili Tika Oktasari Tjipto Leksono Tri Ayu Ningsih Triatma Putri Tutik Wresdiyati Usman Muhammad Tang USMAN PATO Vran Orlando Josua Wahbi, Moh Wahyu Riwanda Widiyanto, Rifki Rahma Wikky Aditiya Putra Wikky Aditiya Putra Windi Ayu Maltadevi Winona Oktania Abriestina Yuliana Yuliana Yusmaida Yusmaida Yusni Ikhwan Siregar yusri munthe yyusmarni yyusmarni Zahtamal Zahtamal Zakiah Zakiah Zulfan Saam