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INAKTIVASI PANAS SPORA BACILLUS CEREUS PADA TAHU Bara Yudhistira; Reny Mailia; Endang S. Rahayu; Yudi Pranoto; Saiful Rochdyanto
Jurnal Teknologi Hasil Pertanian Vol 10, No 1 (2017)
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.381 KB) | DOI: 10.20961/jthp.v10i1.17498

Abstract

As one of perishable food, tofu is susceptible for microorganisms contamination, an effort is needed to solve the problem. Heat treatment is one of the most common methods used to reduce microorganism population. This research aimed to analyze heat treatment effects of Bacillus cereus reduction in tofu. B. cereus isolate was obtained from soy milk cooking process during tofu production in Sudagaran Yogyakarta. D value measurement as parameter analysis was conducted at 80, 90, 100, 110, 120oC. The results showed that D90 value of B. cereus in tofu product was 29.41 minutes D120 value was 1.69 minute, while Z value was 33.33oC. D and Z value obtained was then used as pasteurization reference to extend shelf life of tofu. Pasteurization temperature of 95oC was based on household conventional boiling temperature, and the process time variations were 0, 10, 30 and 34 minutes. During tofu storage at 100C, analyses conducted were Total Plate Count, spore-forming bacteria, and B. cereus enumeration. Sensory parameter was also evaluated; including flavor, color, surface appearance, mucus presence, and texture. The longest shelf life 20 days was obtained by 34 minutes pasteurization and cool storage.
Characteristics of glucomannan of Porang Flour (Amorphophallus oncophyllus) with Various Chemical Extraction Methods Tety Desrita Handayani; Agustina Agustina; Eni Harmayani; Yudi Pranoto
Jurnal Sains dan Teknologi Pangan Vol 8, No 3 (2023): Jurnal Sains dan Teknologi Pangan
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jstp.v8i3.38413

Abstract

Porang plants are widely cultivated in Indonesia as a potential source of glucomannan. Glucomannan is obtained by separating impurity components such as starch, cellulose, protein, fat, and the rest of the flour bag from porang flour. The purpose of this study was to determine the effect of the isolation method on porang flour to produce high purity glucomannan. In this study, the extraction of glucomannan was carried out by a modified simple centrifugation method with the addition of aluminum sulfate (EAS), sodium metabisulfite (ENM), both (ENMAS), and no treatment (EK). All of the glucomannan obtained was then analyzed for composition, color, transparency, degree of acetylation and viscosity. The characteristics of porang glucomannan were then compared with konjac glucomannan (PKG). The results showed that ENMAS showed the highest value with a purity value of 93.71%, WHC 55.06 g water/g glucomannan, whiteness degree 79.16, and gel viscosity 73000 cps.
Physical, Chemical, and Sensory Properties of Ice Cream with the Substitution of Stabilizer with Gelatin from Various Sources Lily Arsanti Lestari; Resha Ayu Wildiana; Fatma Zuhrotun Nisa'; Yuny Erwanto; Yudi Pranoto
Journal of Food and Pharmaceutical Sciences Vol 7, No 3 (2019): J. Food Pharm. Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.702

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Bovine and porcine gelatin are usually used as ice cream stabilizer. However, Muslims areforbidden to consume foods that contain porcine or its derivatives product. Hence, in our previous study, wedeveloped a technique to produce gelatin from local sources such as fish, buffalo, and bovine. This studyaimed to determine the effect of various gelatin on the physical, chemical, and sensory properties of ice cream.Bovine, fish, and buffalo gelatin with the same concentration of 0.3% are added as ice cream stabilizer. Weevaluated the overrun, melting rate, viscosity, as well as the nutrient content. The sensory properties weretested using a hedonic test using a 7-point scale. The substitution of carboxymethyl cellulose (CMC) withvarious gelatin did not affect the overrun, the melting rate, ash and protein content as well as sensoryattributes (p>0.05). On the other hand, ice cream with various gelatin significantly affected the viscosity,moisture, fat, and carbohydrate content (p<0.05).
α-Amilase Inhibitory Activity of Fraction of Ethanolic Extract of Lebui Seed (Cajanus cajan (L.) Millsp.) Grown in West Nusa Tenggara Dian Resti Setyaningrum; Rumiyati; Yekti Asih Purwestri; Satrijo Saloko; Agung Endro Nugroho; Yudi Pranoto; Sri Widyastuti
Journal of Food and Pharmaceutical Sciences Vol 8, No 2 (2020): J. Food Pharm. Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.720

Abstract

Diabetes is a metabolic syndrome characterized by high blood sugar levels or hyperglycemia.Alpha-amylase inhibitors can be used as an antidiabetic that controls postprandial hyperglycemia.Alpha-amylase inhibitors are found in plants as protein and non-protein bioactive content such as in lebuiseed. The purpose of this study was to determine the activity of alpha-amylase inhibition of the fraction ofethanol extract of lebui seed. This research was carried out by extraction of lebui seed and then fractionatedlebui extract to obtain n-hexane fraction (HF), ethyl acetate fraction (EAF), and water fraction (WF). Thefractions then tested to alpha-amylase inhibition activity in vitro by measuring the reducing sugar using3.5-dinitrosalicylic (DNS) reagent. Furthermore, the identification of the bioactive content of each fraction wascarried out using thin layer chromatography. The results of alpha-amylase inhibition activity test showed thatall fractions had the ability to inhibit alpha-amylase. The IC50 value of the n-hexane fraction, ethyl acetatefraction, and water fraction were 173.05 mg/ml, 9.98 mg/ml, and 137.19 mg/ml, respectively. Ethyl acetatefraction has the smallest IC50 value compared to other fractions. Based on the identification of the bioactivecontent, bioactive content that is only found in the ethyl acetate fraction but not in other fractions are tannins.Therefore, the chemical content in lebui seed which acts as an alpha-amylase inhibitor might be tannins.
Identifikasi komponen dan Uji Potensi Anti Jamur Minyak Atsiri Serai Dapur (Cympobogon citratus) Terhadap Jamur Penghasil Aflatoksin Yanti, Rini; Nurdiawati, Hermina; Cahyanto, Muhammad N; Pranoto, Yudi
AGRITEKNO: Jurnal Teknologi Pertanian Vol 9 No 2 (2020): AGRITEKNO: Jurnal Teknologi Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jagritekno.2020.9.2.72

Abstract

Aspergillus flavus and A. parasiticus are food spoilage fungi and produce aflatoxin that causes serious food safety problems. The antifungal agent is needed to control these fungi. This study aimed to determine the component of lemongrass essential oil and to test the oil’s ability to inhibit the growth of A. flavus and A. parasiticus. Five variations in the concentration of lemongrass oil were tested separately on A. flavus and A. parasiticus and, fungal growth was observed for 7 days. The results of the identification of the essential oil components using GC-MS showed that lemongrass essential oil has the main components of citral (53.77%) and z-citral (34.05%). Lemongrass essential oil with various concentrations (0.01%; 0.02%; 0.04% and 0.08%) showed the ability to inhibit the growth of A. flavus and A. parasiticus and the concentration of 0.08% indicated that lemongrass oil could inhibit the growth of both fungi for 100% during 7 days of observation. Keywords: antifungal, aflatoxin, citral, essential oil, lemongrass oil ABSTRAK Aspergillus flavus dan A. parasiticus merupakan jamur yang banyak ditemukan mengkontaminasi pangan. Kedua jamur tersebut menghasilkan aflatoksin, yang berbahaya bagi kesehatan manusia, sehingga diperlukan anti-jamur untuk mengendalikan pertumbuhannya. Penelitian ini bertujuan untuk mengetahui komponen minyak atsri serai (Cymbopogon citratus) dan menguji kemampuan minyak tersebut sebagai penghambat pertumbuhan A. flavus dan A. parasiticus. Lima variasi konsentrasi minyak serai diujikan secara terpisah pada A. flavus dan A. parasiticus dan dilakukan pengamatan pertumbuhan jamur selama 7 hari. Hasil identifikasi komponen minyak atsiri menggunakan GC-MS menunjukkan bahwa minyak atsri serai memiliki komponen utama citral (53,77%) dan z-citral (34,05%). Minyak atsiri serai dengan berbagai konsentrasi (0,01%; 0,02%; 0,04% dan 0,08%) menunjukkan kemampuan menghambat pertumbuhan A. flavus dan A. parasiticus dan konsentrasi 0,08 % menunjukkan bahwa minyak serai mampu menghambat pertumbuhan kedua jamur sebesar 100 % selama 7 hari pengamatan Kata kunci: antijamur, aflatoksin, minyak atsiri, minyak serai, sitral
Karakteristik Fisik dan Fungsional Pati Keladi (Xanthosoma sagittifolium) dari Beberapa Lokasi di Maluku Lopulalan, Cynthia G C; Marseno, Djagal W; Marsono, Yustinus; Pranoto, Yudi
AGRITEKNO: Jurnal Teknologi Pertanian Vol 10 No 1 (2021): AGRITEKNO: Jurnal Teknologi Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jagritekno.2021.10.1.17

Abstract

This study aimed to characterize the physical and functional properties of cocoyam starch from several locations in Maluku. The use of starch in the processed food and non-food industries will be determined by its various characteristics depending on the starch source. It is expected from this research that data can be used to assess the potential of cocoyam starch in its use in the food and non-food industries. Cocoyam tubers from Maluku, i.e. Buru Island, Saparua Island, and Saumlaki Island were used as samples in this research.. The wet extraction method was used to produce cocoyam starch in this research. Physical properties such as tuber length and diameter, tuber skin color, yield were analyzed. Swelling power and solubility, water holding capacity (WHC), paste clarity, pH and color were among functional properties observed. The results showed that the length of cocoyam tubers from the three sample locations was in the range of 8.75-36 cm with a diameter of 8-22 cm. The skin color of the tubers was found to be blackish-brown, and the color of the tubers is white with a starch yield of 12-16%. Based on the statistical analysis results, starch's functional properties like swelling power, solubility, WHC, paste clarity, pH, and color for the three starches were not significantly different from one another. This shows that differences in growing locations within an archipelago did not affect cocoyam starch's functional properties. Keywords: Cocoyam starch, functional property, location, physical property ABSTRAK Tujuan dari penelitian adalah mengkarakterisasi sifat fisik dan fungsional pati keladi yang berasal dari beberapa lokasi di Maluku. Karakteristik yang berbeda berdasarkan sumber pati akan menentukan kesesuaian pemanfaatannya dalam industri pangan olahan maupun non pangan. Diharapkan dari penelitian ini adalah diperoleh data yang dapat digunakan untuk mengkaji potensi pati keladi dalam penggunaanya di industri pangan maupun non pangan. umbi keladi yang digunakan sebagai sampel dalam penelitian ini berasal dari dari lokasi di Maluku yaitu umbi keladi asal pulau Buru, pulau Saparua dan pulau Saumlaki. Metode ekstraksi yang di gunakan dalam penelitian ini untuk menghasilkan pati keladi adalah metode ekstraksi basah. Parameter yang dianalisis meliputi sifat fisik yaitu panjang dan diameter umbi, warna kulit umbi, rendemen; serta sifat fungsional yaitu swelling power dan sollubility, water holding capacity (WHC), kejernihan pasta, pH, warna. Hasil yang diperoleh adalah panjang umbi keladi dari tiga lokasi sampel adalah 8,75-36 cm dengan diameter 8-22 cm. Warna kulit umbi coklat kehitaman dan warna umbi putih serta rendemen pati 12-16%. Berdasarkan hasil analisis statistik, sifat fungsional pati yaitu swelling power, sollubilty, WHC, kejernihan pasta, pH dan warna untuk ketiga pati tidak berbeda nyata satu dengan lainnya. Hal ini menunjukkan bahwa perbedaan lokasi tumbuh dalam satu kepulauan tidak mempengaruhi karakteristik fungsional pati keladi yang diteliti. Kata kunci: Pati keladi, lokasi, sifat fisik, sifat fungsional
PERHITUNGAN PRODUKTIFITAS DAN ANGGARAN BIAYA ALAT BERAT PEKERJAAN RIGID PAVEMENT JALAN SEBULU MENUJU JALAN PROVINSI STA 0+000 SAMPAI DENGAN STA 5+400 KABUPATEN KUTAI KARTANEGARA Sulthon, Arian Lutfi Nur; Dhiva, IGN Aditya; Pranoto, Yudi
JURNAL INERSIA Vol. 10 No. 1 (2018): Jurnal Inersia
Publisher : POLITEKNIK NEGERI SAMARINDA

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Abstract

Tujuan dari penulisan tugas akhir ini adalah untuk menghitung produktivitas alat berat, anggaran biaya, biaya operasional milik sendiri dan sewa. Perhitungan produktivitas dimulai dengan menghitung volume pekerjaan lapis pondasi, volume pekerjaan perkerasan kaku, dan menghitung anggaran biaya alat. Biaya operasional alat berat tersebut dihitung dengan dua asumsi yaitu milik sendiri dan sewa. Dari perhitungan tersebut didapatkan volume lapis agregat pondasi, volume perkerasan kaku, produktivitas alat berat, hari pekerjaan alat berat, biaya alat berat perjam dan biaya keseluruhan alat berat. Dari perhitungan yang telah dilakukandidapatkan durasi 80 hari atau 13,42 minggu, operasional alat berat milik sendiri sebesar Rp. 1.935.677.217 sedangkan dengan biaya sewa yaitu sebesar Rp. 1.659.853.741.
PERENCANAAN BETON BERTULANG MENGGUNAKAN PROGRAM ETABS 2016 DAN TEKLA STRUKTUR 2017i PADA SDN 027 LOA JANAN ILIR SAMARINDA Saputra, Muhammad Tomy; Pranoto, yudi; Ridwan, M.
JURNAL INERSIA Vol. 10 No. 2 (2018): Jurnal Inersia
Publisher : POLITEKNIK NEGERI SAMARINDA

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Abstract

Tujuan dari perencanaan ini adalah untuk menghitung dan mendetailkan struktur element pelat, balok, dan kolom gedung sdn 027 loa janan ilir. Perhitungan struktur beton dimulai dengan menghitung pembebanan lalu di input kedalam ETABS 2016 dan didapatkan gaya-gaya dalam berupa momen (M), gaya lintang (D) dan gaya normal (N). Kemudian hasil analisa gaya dihitung menggunakan metode SNI 03-2847-2013 dan mengacu pada PPIUG 1983. Dari perhitungan tersebut didapatkan hasil momen kolom yaitu PU = 33679,08 kg.cm MU = 250962,3 kg.cm dengan jumlah tulangan 6 D 16, pada balok MUtumpuan = 385004,09 MUlapangan 192502,04 kg.cm dengan jumlah tulangan lapangan 3 D 13 dan tumpuan 3 D 13 dengan jarak sengkang tumpuan Ø10 - 125 mm dan lapangan Ø10 - 250 mm dan pada pelat lantai didapat jarak tulangan arah x Ø10 - 150 mm, tulangan arah y Ø10 – 250 mm.
Maltodextrin from Cassava Starch (Manihot Utilissima) and Its Application as Encapsulating Agent of Red Guava Juice Extract (Psidium Guajava) Krisnitya, Widya; Darmadji, Purnama; Pranoto, Yudi
Journal of Agri-Food Science and Technology Vol. 1 No. 1 (2020): February
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.178 KB) | DOI: 10.12928/jafost.v1i1.1861

Abstract

Cassava starch is one of the processed products of cassava, which can be made into maltodextrin. Maltodextrin is often applied to the encapsulation method of instant drink packaging in Indonesia. One of the most favorite fruits in the instant drink industry is red guava. The research objectives are to know the characteristic of maltodextrin made by hydrolysis of cassava starch, and to identify the effects of the amount of maltodextrin added to the characteristic of red guava extract produced. In the research, the hydrolysis time of cassava starch is varied into 60, 90, and 120 minutes. Maltodextrin made will be analyzed, and its characteristics on the encapsulation of red guava extract will be defined. The red guava juice is encapsulated by the variants of maltodextrin concentration as 10%, 15%, 20%, and 25%. Red guava extract powder produced from the selected amount of maltodextrin then be analyzed for its characteristics. The results showed that the highest DE was found in maltodextrin with 120 minutes hydrolysis time. The increased amount of maltodextrin added affects the increase of solubility and vitamin C levels of the produced red guava juice extract. The water content is not affected by the amount of maltodextrin added.
Karakteristik Amilografi dan Sifat Pengembangan Tepung Singkong pada Optimasi Modifikasi Kombinasi Asam Laktat-Perlakuan Panas Lembap Widanti, Yannie Asrie; Murdiati, Agnes; Pranoto, Yudi; Fibri, Dwi Larasatie Nur
Jurnal Teknologi dan Industri Pangan Vol. 36 No. 1 (2025): Jurnal Teknologi dan Industri Pangan
Publisher : Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2025.36.1.1

Abstract

Although original properties of local flour have limited their uses in bread making, modification potentially enables them to become a viable option in the making of bakery products, aiming to substitute wheat flour. This study aimed to determine the optimum condition of lactic acid-HMT treatments for cassava flour. The amylographic characteristics of the flour and its effects on bread expansion were observed. A five-factor Box-Behnken design (lactic acid concentration, reaction time, HMT temperature, HMT time, and flour water content) was used, while the resulting data were analyzed using linear regression in Minitab software at a 5% significance level. The results showed that the treatment yielding modified cassava flour with high baking expansion was obtained at 0.5% lactic acid, 60 min reaction time, 30% flour water content, and temperature 140 °C for 1 h. Moreover, remarkable changes in pasting properties were observed after modification. The treatment increased peak, trough, and final viscosity but decreased breakdown viscosity. The morphology of modified flour differed from native one, with the former exhibiting more hollow granules and a flake-like structure.
Co-Authors Agnes Murdiati Agnes Murdiati Agung Endro Nugroho Agustina Agustina Amir Husni Angwar, Mukhamad Ariani, Dini Ariani, Dini Ashri Mukti Benita Bambang Setiaji Bara Yudhistira Bara Yudhistira Cahyanto, Muhammad N Darmadji, Purnama Dego Yusa Ali Dewi, Angela Myrra Puspita Dhiva, IGN Aditya Dian Resti Setyaningrum Dimas Rahadian Aji Muhammad, Dimas Rahadian Aji Dini Ariani Djagal Marseno Djagal W Marseno Djagal Wiseso Marseno Djagal Wiseso Marseno Djagal Wiseso Marseno Djagal Wiseso Marseno Doddy Sutono Dodik Suprapto Edowai, Desi Natalia Eduard Fransisco Tethool Elfanti, Maharani P. Elok Pawening Maharani Endang S. Rahayu Eni Harmayani Eni Harmayani Eni Harmayani Eni Harmayani Ervika Rahayu Novita Herawati Fatchul Anam Nurlaili Fatma Zuhrotun Nisa Fela Laila Ferdiansyah, Mokhamad Khoiron Fibri, Dwi Larasatie Nur Hafni Rahmawati Halim, Lina Haryadi Haryadi Haryadi Haryadi Hayati, Febsi Hermina Nurdiawati Herry , Herry Isak Silamba Joko Nugroho Wahyu Karyadi Joko Nugroho Wahyu Karyadi Karlina, L Lola Krisnitya, Widya Lily Arsanti Lestari Lopulalan, Cynthia G C M. Ridwan Made Darawati Miftakhussolikhah, Miftakhussolikhah Mimin Yulita Kusumaningrum Muhammad Nur Cahyanto nFN Miftakhussolikhah nFN Nurliyani Novianty, Hilda Novianty, Hilda Nur Rohmah Lufti A'yuni Nurdiawati, Hermina Nurfitri Ekantari Nuri Arum Anugrahati Nursigit Bintoro Nursigit Bintoro Nursigit Bintoro Prawira, Muhammad Aditya Puji Wulandari Purnama Darmadji Purnama Darmadji Purnama Darmadji Purnama Darmadji Purnama Darmadji Rahayu, Endang Sutriswati Rahayu, Ervika Rahma Bayunita Hapsari Reny Mailia Reny Mailia Resha Ayu Wildiana Retno Ayu Kurniasih Rini Yanti Rini Yanti, Rini RNH, Ervika Rosiana, Nita Maria Rumiyati Sabrina, A'isyah Mutiara Saiful Rochdyanto Saiful Rochdyanto Saputra, Muhammad Tomy Sarman Oktovianus Gultom Satrijo Saloko Siti masithah Fiqtinovri Sri Hartati Sri Hartuti Sri Hartuti Sri Hartuti Sri Raharjo Sri Widyastuti Sri Widyastuti Sri Winarti Stefani Amanda Harmani Subaryono Subaryono Subaryono Subaryono, Subaryono Sudibyo, Anung Sudip Kumar Rakshit Sugeng Pondang Sugiharto Suharwadji, - Suharwadji, - Sulthon, Arian Lutfi Nur Supriyadi Supriyadi Supriyanto Supriyanto Tanwirul Millati Taswir Taswir Tazwir Tazwir Tety Desrita Handayani Triwitono, Priyanto Tyas Utami Umar Santoso Ustadi Ustadi Ustadi, Ustadi Wardah Wardah Wulandari, Pudji Yekti Asih Purwestri Yuny Erwanto Yustinus Marsono Yustinus Marsono Yustinus Marsono Yustinus Marsono