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All Journal ALCHEMY Jurnal Penelitian Kimia Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Ilmu Kefarmasian Indonesia AKURASI: Jurnal Riset Akuntansi dan Keuangan SULUH: Jurnal Abdimas Magistrorum et Scholarium: Jurnal Pengabdian Masyarakat Capacitarea : Jurnal Pengabdian Kepada Masyarakat Kartika : Jurnal Ilmiah Farmasi Jurnal Sains dan Kesehatan Akurasi
Liliek Nurhidayati
UNIVERSITAS PANCASILA
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Validation of HPLC for identification of captopril, enalapril, and lisinopril in traditional Indonesian herbal medicine Liliek Nurhidayati; Mawarni Tobing; Elvi Rahmayuni
JURNAL ILMU KEFARMASIAN INDONESIA Vol. 24 No. 1 (2026): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v24i1.2041

Abstract

Traditional Indonesian herbal medicine (Jamu) is widely used for health maintenance and disease  prevention. The addition of medicinal chemicals to Jamu is not in accordance with quality and safety requirements. The purpose of this study was to develop a valid HPLC method for the identification of captopril, lisinopril, and enalapril in Jamu for hypertension. In this study, they were identified using high-performance liquid chromatography with a photodiode array detector. The stationary phase was a C18 YMC column (length 250 mm, diameter 4.6 mm, particle size 5 μm), methanol - 0.1 % phosphoric acid (45:55) as the mobile phase with isocratic elution, a flow rate of 1.0 mL/min, a column temperature of 40 °C, an injection volume of 20 μL, and detection at  210 nm. The results showed that this method fulfilled the specificity requirement, with detection limits of captopril, enalapril, and lisinopril of 0.715, 0.795, and 1.403 ppm, respectively. This HPLC method can be used for the identification of captopril, enalapril, and lisinopril in commercial Jamu.
Optimasi dan Validasi Metode Kromatografi Cair Kinerja Tinggi untuk Menetapkan Kadar Asam Klorogenat dalam Ekstrak Etanol Daun Yakon (Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson) Aziz, Zuhelmi; Nurhidayati, Liliek; Abdillah, Syamsudin; Yuliana, Nancy Dewi; Simanjuntak, Partomuan
ALCHEMY Jurnal Penelitian Kimia Vol 16, No 1 (2020): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4044.619 KB) | DOI: 10.20961/alchemy.16.1.28952.67-76

Abstract

Yakon merupakan tanaman yang dapat digunakan untuk pengobatan dan kebutuhan pangan. Salah satu kandungan zat berkhasiat dalam daun yakon adalah asam klorogenat. Asam klorogenat diketahui memiliki aktifitas sebagai antioksidan, antikanker dan antidiabetes.  Penentuan kadar asam klorogenat dalam matriks yang kompleks diperlukan metode yang selektif dengan ketelitian dan ketepatan yang baik. Pada penelitian ini dilakukan optimasi dan validasi metode kromatografi cair kinerja tinggi (KCKT) fase balik untuk penetapan kadar asam klorogenat. Ekstrak dibuat secara ultrasonikasi menggunakan pelarut etanol 95%. Kondisi optimum diperoleh menggunakan fase gerak asam format 0,1% dalam asetonitril–asam format 0,1% dalam air (gradien); fase diam oktadesilsilan (C18) pada suhu 30 ºC dan detektor UV pada panjang gelombang 328 nm. Metode KCKT ini memberikan hasil yang memiliki ketelitian yang tinggi dengan simpangan baku relatif 0,79% dan ketepatan yang baik dengan perolehan kembali 97,50%. Kadar asam klorogenat yang diperoleh dalam ekstrak etanol 95% daun yakon sebesar 1,02%. Optimization and Validation of High Performance Liquid Chromatography Methods for Determination of Chlorogenic Acid Levels in Ethanol Extracts of Yakon Leaves (Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson). Yacon is a plant that can be used for medication and food needs. One of the bioactive compounds of yacon leaves is chlorogenic acid. Chlorogenic acid has various biological activities, such as antioxidant, anticancer and antidiabetic activities. To determine the chlorogenic acid in such complex matrix, such selective methods with good precision and acuracy are required. In this study, the optimization and validation of reverse phase high performance liquid chromatography (HPLC) method for chlorogenic acid determination were performed. The extract was prepared by ultrasonication in 95% ethanol.The optimized condition for HPLC obtained was by using mobile phase  0.1% formic acid in acetonitrile – 0.1% formic acid in water with gradient elution, stationary phase octadesylsilane  (C18)  at 30 oC and UV detector at of 328 nm. The result showed that HPLC method had high precicion with relative standard deviation of 0.79% and high accuracy with recovery of 97.50%. The chlorogenic acid in the ethanol 95% extract of Yacon leaves was 1,02%.
Co-Authors . Syamsudin Agus Purwanggana Bagus Bahtiar Deni Rahmat Diah Kartika Pratami Dian Ratih Laksmitawati Dianita Ayu Noviastuti Elvi Rahmayuni Esti Mumpuni Esti Mumpuni Esti Mumpuni Farida Ariyani FRANSISKA DIANA SANTI I WAYAN REDJA Kharinta Adella Meynderth Laili Savitri Noor Martati, Titiek Marwati, Umi Mawarni Tobing Meilda Ayu Bathini Moch Futuchul Arifin Mohamad Rafi Mulatsari, Esti Nancy Dewi Yuliana Ni Made Dwi Sandhiutami Nofanti Sandra Noor, Laili Savitri Partomuan Simanjuntak Reczky Muhammad Ramdhan Hasan Riska Eka Putri, Riska Eka ROS SUMARNY Siti Sofiah Syamsudin Abdillah Umi Marwati Yashinta Fitriani YATI SUMIYATI Yesi Desmiaty, Yesi Zaidan, Sarah Zuhelmi Aziz