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Journal : agriTECH

Distribusi Plumbum, Cadmium pada Biji Kedelai, dan Deprotonasi Gugus Fungsional Karboksil Asam Sitrat dalam Khelasi Sapto Priyadi; Purnama Darmadji; Umar Santoso; Pudji Hastuti
agriTECH Vol 34, No 4 (2014)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.627 KB) | DOI: 10.22146/agritech.9435

Abstract

A study on the distribution of plumbum, cadmium on soybeans and deprotonation of carboxyl functional groups of citric acid in the chelation process. This study was deprotonation phase of carboxyl functional groups of citric acid as chelating agent, that was review chemically activity (protons number) functional group of citric acid was caused of chelation environment pH changes. Research factors were 1) ratios of citric acid with water, consists of three levels i.e. 0,1:1; 0,2:1 and 0,3:1 second factors was chelation environment pH, consists of three levels i.e. 5; 7.5 and 10. The results showed that, the plumbum (Pb) accumulation in seed coat tissue average value 0,37 ± 0,03 ppm, whereas Pb  on cotyledon tissue average value 0,39 ± 0,07 ppm. Cadmium (Cd) accumulation in seed coat tissue average value  was 0,02 ± 0,004 ppm while the Cd on cotyledon tissue average value 0,02 ± 0,003 ppm. The highest deprotonation of functional groups citric acid as chelating agent 9,43cmol+kg was reached on chelation condition citric acid ratios with water 0,3:1 on chelation environment pH 10 (A3B3–1). The study result non significant difference with citric acid ratios with water 0,2:1 and 0,1:1 on chelation environment pH 10.ABSTRAKTelah dilakukan penelitian tentang distribusi plumbum, cadmium pada biji kedelai dan deprotonasi gugus fungsional karboksil asam sitrat dalam proses khelasi. Penelitian ini merupakan tahapan deprotonasi gugus fungsional karboksil asam sitrat, yaitu tinjauan secara kimiawi keaktifan (jumlah proton) gugus fungsional asam sitrat akibat perubahan pH lingkungan khelasi. Faktor penelitian meliputi  ratio asam sitrat terhadap air yang terdiri dari tiga taraf 0,1:1, 0,2:1 dan 0,3:1 dengan pH lingkungan khelasi yang terdiri dari tiga taraf 5; 7,5 dan 10. Hasil penelitian menunjukkan bahwa, akumulasi plumbum (Pb) pada jaringan kulit biji kedelai rerata 0,37 ± 0,03 ppm, sedangkan Pb pada jaringan kotiledon rerata 0,39 ± 0,07 ppm. Akumulasi cadmium (Cd) pada jaringan kulit biji kedelai rerata 0,02 ± 0,004 ppm sedangkan Cd pada jaringan kotiledon rerata 0,02 ± 0,004 ppm. Deprotonasi gugus fungsional chelating agent asam sitrat tertinggi 9,43 cmol + kg –1 dicapai pada kondisi khelasi dengan ratio asam sitrat terhadap air 0,3:1 pada lingkungan khelasi pH 10 (A). Keadaan tersebut tidak berbeda nyata dengan perlakuan ratio asam sitrat 0,2:1 dan 0,1:1 pada lingkungan khelasi pH 10.
Khelasi Plumbum (Pb) dan Cadmium (Cd) Menggunakan Asam Sitrat Pada Biji Kedela Sapto Priyadi; Purnama Darmadji; Umar Santoso; Pudji Hastuti
agriTECH Vol 33, No 4 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.818 KB) | DOI: 10.22146/agritech.9536

Abstract

A study on the chelation of plumbum and cadmium by citric acid in soybean seeds, it was aimed to identify the effect of chelation treatment (chelating ratio, time contact and seeds condition) on plumbum reduction and cadmium in soybean seeds. A factors of research covering 1st) chelating ratio (citric acid/”C”) consisting of three (1, 2 and 3 grams of citric acid); 2nd) the time contact (“D”) consisting of three (90, 120 and 180 minutes) and the 3rd factors is condition of the seeds (“E”) consisting of three standard (whole qualify e.i. passing sieve of 0.3 inch and restrained at 0.2 inch), ruptured e.i. restrained for sieve at 10 mesh, and flour qualify for pass for sieves 50 mesh. The result showed that the combination of three factors were able to reduce Cd maximaly (non detected/detect limit 0.01 ppm). Reduction Pb maximaly (non detected /detect limit 0.09 ppm) occured in treatment C1D1E1, C1D1E2, C1D2E1, C1D2E2, C1D2E2, C2D1E1, C2D1E2, C2D2E1, C2D3E1, C2D3E2, C3D1E1 and C3D2E1. A combination of citric acid chelation (C) with the time contact (D) showing significant difference and both showed interactions, the reduction of the highest was C1D1 treatment. A combination of citric acid chelation (C) with the condition of the nut (E) showed significant difference and both no interaction, the reduction of the highest was C2E1 treatment. The combination between the time of contact (D) with the condition of the seed (E) showed significant difference and both no interaction, the reduction of the highest were D1E1 and D2E1 treatments.ABSTRAKTelah dilakukan penelitian tentang khelasi plumbum (pb) dan cadmium (cd) menggunakan asam sitrat pada biji kedelai, dengan tujuan untuk mengetahui pengaruh faktor perlakuan khelasi (ratio pengkhelat, waktu kontak dan kondisi biji) dalam mereduksi Pb dan Cd pada biji kedelai. Faktor penelitian meliputi 1) rasio pengkhelat (asam sitrat/C) yang terdiri dari tiga taraf (1, 2 dan 3 gram asam sitrat); 2) waktu kontak (D) yang terdiri dari tiga taraf (90, 120 dan 180 menit) serta faktor ke-3) kondisi biji (E) yang terdiri dari tiga taraf (utuh yaitu lolos pada saringan 0.3 inch dan tertahan pada 0.2 inch, pecah yaitu tertahan pada saringan 10 mesh dan tepung yaitu lolos pada saringan 50 mesh. Hasil penelitian menunjukkan bahwa kombinasi ketiga faktor, mampu mereduksi Cd secara maksimal (tidak terdeteksi/batas deteksi 0,01 ppm). Reduksi Pb tertinggi adalah maksimal (tidak terdeteksi/batas deteksi 0,09 ppm) terjadi pada perlakuan C1D1E1, C1D1E2, C1D2E1 dan C1D2E2; C2D1E1, C2D1E2, C2D2E1, C2D3E1 dan C2D3E2; C3D1E1 dan C3D2E1. Kombinasi antara pengkhelat ”asam sitrat” (C) dengan waktu kontak (D) menunjukkan berbeda nyata dan keduanya menunjukkan interaksi, reduksi tertinggi dicapai perlakuan C1D1. Kombinasi antara pengkhelat ”asam sitrat” (C) dengan kondisi biji (E) menunjukkan berbeda nyata dan keduanya tidak ada interaksi, reduksi tertinggi dicapai perlakuan C2E1. Kombinasi antara waktu kontak (D) dengan kondisi biji (E) menunjukkan berbeda nyata dan tidak ada interaksi, reduksi tertinggi dicapai perlakuan D1E1 dan D2E1.
Identifikasi Logam Berat dalam Biji Jagung Manis dan Kedelai pada Transisi Sistem Pertanian Organik Sapto Priyadi; Soelistijono Soelistijono; Setie Harieni; Kusriani Prasetyowati
agriTECH Vol 38, No 4 (2018)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.274 KB) | DOI: 10.22146/agritech.24739

Abstract

Heavy metal contaminants in land agriculture are environmental problems, and they affect food safety, so there is a need for the research of Pb, Cd and Cu decrease absorption by plants using organic chelating agent, i.e. substance of humus (humic and fulvic acid) in the manure. The research was done with a completely block design, in Gagaksipat, Ngemplak, Boyolali; heavy metal analysis in manure, land and seeds used an atomic absorption spectrophotometer-flame (AAS Jena Contr AA 300, Germany). It was aimed to identify contaminants of Pb, Cd, and Cu in sweet corn and soybean seeds. The research treatments were doses of cow manure: 15, 20, 25, 30, 35, 40, 45 and 50 kg per hectare. The results showed that the enrichment coefficient (EC) of Pb and Cu in sweet corn seeds had low accumulator plants categories; the EC of plumbum in soybean seeds had low accumulator plants categories, whereas the EC of copper soybean seeds had moderate accumulator plants categories; and the EC of cadmium in the sweet corn and soybeans seeds was undetectable. Exposures of Pb and Cd in corn sweet and soybean seeds were undetectable, while exposures of Cu in sweet corn and soybean seeds were 2.03 and 12.39 ppm, respectively. Contamination of Pb, Cd and Cu exposures in land after sweet corn harvested were 30.99; undetectable and 60.58 ppm, respectively. While Pb, Cd and Cu residues in land after soybean harvested were 33.24; undetectable and 56.26 ppm, respectively.