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All Journal Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Proceeding Seminar LPPM UMP Tahun 2014 Sain Akuatik E-Dimas: Jurnal Pengabdian kepada Masyarakat JRST (Jurnal Riset Sains dan Teknologi) Jurnal Gema Ngabdi Community Development Journal: Jurnal Pengabdian Masyarakat Proceedings Series on Physical & Formal Sciences Society: Jurnal Pengabdian Masyarakat Jurnal WIDYA LAKSMI (Jurnal Pengabdian Kepada Masyarakat) Abdimas Indonesian Journal Prosiding University Research Colloquium Journal of Fish Health JPEMAS: Jurnal Pengabdian Kepada Masyarakat
Cahyono Purbomartono
Faculty of Fisheries and Marine Science, Muhammadiyah University of Purwokerto
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Journal : Journal of Fish Health

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Isolation and Molecular Identification of Pathogenic Bacteria in Giant Gourami (Osphronemus gouramy) Cultivated in Tamansari and Singasari Villages, Banyumas Regency Lenterani, Febiana; Purbomartono, Cahyono; Pratama, Ikhsan
Journal of Fish Health Vol. 6 No. 2 (2026): Journal of Fish Health
Publisher : Study Program of Aquaculture, Department of Fisheries and Marine Science, Faculty of Agriculture, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v6i2.9837

Abstract

Giant gourami (Osphronemus gouramy) is a freshwater fishery commodity with high economic value and is widely cultivated in Banyumas Regency. However, the emergence of diseases caused by pathogenic bacteria often becomes an obstacle in cultivation activities because it can reduce the survival rate and productivity of fish. This study aims to identify the types of pathogenic bacteria found in gouramy fish raised in Tamansari and Singasari Villages, Banyumas Regency. The research method was carried out by taking liver samples of gouramy fish which were then isolated on TSA media. Identification was carried out based on the morphological characteristics of bacterial colonies, the Gram KOH test, and molecular identification. The results showed that from both locations, several bacterial isolates were obtained with similar colony morphological characteristics, namely round (circular), small size, smooth edges, smooth surface, and cream color. The results of the Gram KOH test showed that all isolates were Gram-negative bacteria. Based on these characteristics, the isolates have similarities with the genus Acinetobacter which is often found as an opportunistic bacteria in freshwater fish. The results of molecular identification also showed that the bacteria obtained from the cultivated gouramy fish were Acinetobacter sp. This study provides a preliminary overview of the presence of opportunistic bacteria such as Acinetobacter sp., which have the potential to become pathogens in gourami when water quality conditions are unstable.
Study of the Presence of Streptococcosis in Tilapia Cultivation in Banyumas Regency Saputra, Kukuh Andre Setya; Purbomartono, Cahyono; Pratama, Ikhsan; Mulia, Dini Siswani; Rijal, Muhammad Azharul
Journal of Fish Health Vol. 6 No. 2 (2026): Journal of Fish Health
Publisher : Study Program of Aquaculture, Department of Fisheries and Marine Science, Faculty of Agriculture, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v6i2.9839

Abstract

Tilapia is the most widely cultivated fish in Banyumas which is susceptible to bacterial diseases caused by the increasing number of disease cases in tilapia (Oreochromis niloticus) due to high cultivation intensification. One of the diseases that often attacks is streptococcosis caused by the bacteria Streptococcus sp., which can cause mass mortality and major economic losses for farmers. The purpose of this study was to identify pathogenic bacteria in farmed tilapia in Banyumas. The method used in this study was a purposive sampling method, namely by taking samples of tilapia that have characteristics of disease symptoms such as protruding eyes, damaged fins, and red wounds on the scales from three locations, namely Beji Village, Karangsalam Village, and Pasir Wetan Village. Bacterial samples were obtained from four organs, namely the brain, kidneys, eyes, and liver, then observed the morphology of bacterial colonies and Gram KOH tests were carried out. The results showed that bacterial colonies found in most organs were round to irregular in shape, white to yellowish in color, and Gram positive, which shows the characteristics of Streptococcus sp. bacteria. Based on these results, it can be concluded that Streptococcus sp. bacteria are still the main threat in tilapia cultivation in Banyumas, so it is necessary to take preventive measures through the implementation of good biosecurity, the use of immunostimulants, and the development of vaccines to maintain the sustainability of tilapia cultivation businesses, especially in Banyumas Regency.
Co-Authors . Suwarsito Aditya Maulana, Aditya Bagis, Fatmah Budi, Larasati Ika Dini Siswani Mulia Eka Yulyanti, Eka Filardhi, Mohamad Noufal Heri Maryanto Hery Maryanto, Hery Ikhsan Pratama Jejentri, Jejentri Juli R. Wuliandari, Juli R. Lenterani, Febiana Miftakhul Mudah, Miftakhul Mochammad Imron Awalludin Nabil, Imam Chaerul Pribadi, Teguh Prihandoko, Arief Rijal, Muhammad Azharul Rusman, Ade Saputra, Kukuh Andre Setya Tumisem Tumisem Tumisem, T Wakhudin Wakhudin, Wakhudin Widjarnako, Beny Widya Apriyanti, Widya Wijanarko, Beny R Wijarnako, Raden Beny Wijarnako3, Raden Beny Yanuar Verianto, Yanuar