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Journal : SAINS DAN MATEMATIKA

The Potential Test of Fungal Antagonist Trichoderma viride to inhibit the Growth of Pathogenic Fungi Fusarium moniliforme and Alternaria solani In-Vitro Purwantisari, Susiana; Evendi, Agus
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 3 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Fusarium moniliforme and Alternaria solani are two types of mold which often cause the diseases of cultivated plants. Fusarium moniliforme causes the ear rot disease on corn and the wilt disease of Solanaceae family. Whereas the pathogenic fungus Alternaria solani causes an early bright disease on the onion and potato. This aim of this study was to determine the ability of fungal antagonist Trichoderma viride in inhibiting the growth of Fusarium moniliforme and Alternaria solani in vitro. The growth inhibition ability test were conducted on dual cultures by growing the fungal antagonists with pathogenic fungi in Petri dish containing potato dextrose agar media face-to-face in a distance of 3 cm. Percentages of the growth inhibiting were observed every day in 7 days incubation. The results showed that the fungal antagonist T. viride exhibited the highest inhibition on F. moniliforme in 3 days incubation period which was 63.07 %. Yet the highest inhibition against A. solani was in 2 days incubation period which was 57.35 %. T. viride growth continued to increase since the first day until the seventh day incubation period but contrarily the growth of both pathogenic fungi underwent inhibition. This suggested that T. viride was potential as a biological control agent of F. moniliforme and A. solani growth and have a potency as an active bio fungicide ingredient. Keywords: Dual culture; percentage inhibiting; Trichoderma viride; Alternaria solani; Fusarium moniliforme
Isolation and Identification of Mold Contaminants on Mushroom Growing Medium (Bag Log) and Their Cellulolytic Performance Test Handayani, Tatik; Purwantisari, Susiana
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 2 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Mushrooms naturally grows on logs that have experienced weathering. Raw materials of mushroom growth media containing plenty of wild microbes, especially wild mold on sawdust media. Sterilization conducted did not prevent the occurrence of contamination. This study aim was to determine the types of contaminant molds in cultivated mushroom growth media (bag logs), as well as determine their cellulolytic performance. Mushroom growth media (bag log) samples were taken from three mushroom cultivation locations. Mold isolates obtained were identified by their macroscopic and microscopic characteristics. Test the mold cellulolytic performance was conducted by measuring the ratio of hydrolysis zone with the diameter of mold on CMC (Carboxy Methyl Cellullose) media. The isolation and identification results of contaminant molds consisted of mold isolates which could be grouped into 7 genus which were Aspergillus, Penicillium, Paecillomyces, Trichoderma, Rhizopus, Fusarium and Syncephalastrum. The cellulolytic performances of mold on CMC media showed that Aspergillus flavus has the greatest cellulolytic ability.
Co-Authors Achmadi Priyatmojo Agus Evendi, Agus Ahmad Arfi Setiadi Ahmad Hanafi Ahmad Thontowi Akbar, Muhammad Falih Amal, Daffa Ikhlasul Amatullah, Lutfiana Hary Ana Bila Nadia Anindya Ardiansari Annisa Dhea Lathifa Anto Budiharjo Arfieta, Frisca Arina Tri Lunggani Astriana, Novi Atik Mawarni Azizah, Hana Fitria Bodhicitta Wardaja Budi Raharjo Budi Raharjo Budi Setiyana Budi Setiyana Budihardjo, Kadarwati Chatarina Umbul Wahyuni Choiriyah, Miftahul Dafip, Muchamad Dewi Fajar Kharisma Dion, Romario Dwi Handayani Dwi Handayani Eflita Yohana Elvi Yetti, Elvi Fikri, Dzakiyya Nabilla Fiva Andriyani Govinda Restu Syahputra Hapzi Ali Harum Sitepu Harwika Ria Rizky Agista Hermawan Dwi Ariyanto Hermawan Dwi Ariyanto Herry Wirianata Husna, Faza Laili Ilyas Teguh Pangestu Indriana Putri Inggrit Amedia, Inggrit Jaya, Myra Ivana Yulia Kadarwati Budiharjo Karnoto, K Khanifah, Neni Nur Lintang Dian Saraswati M. Arie Wuryanto Mahardhika, Wahyu Aji Martini Martini MG Isworo Rukmi Mohamad Endy Y Yulianto Mufida, Naimatul Muhammad Bismar Iskandar putra Naufal, Mochammad Fa’iq Qoys Noviyanti, Nita Nurbayani, Farah Arhusy Nurul Halwiyah Pangestu, Ilyas Teguh Purwoko, Agus Puspitasari, Elsye Giovanny Rahmadita, Tika Ramadhan, Akhmad Andika Rasyid, Rido Baharudin Ar Rejeki Siti fe Rejeki Siti Ferniah Retno Hestiningsih RETNO PENI SANCAYANINGSIH Retno Peni Sancayaningsih Rina Sri Kasiamdari Ririh Citra Kumalasari, Ririh Citra Risnanda, Meizulfa Ayu Rizka Amalia Rizky Wahyu Saputro Safina, Marista Fikri Irsya Sari, Dyah Maharani Sisya Puspita Sari, Rachel Dewita Sarjana Parman Septa Primananda Sitepu, Harum Siti Nur Jannah Siti Nur Jannah Sofatun Misrofah Sri Pujiyanto Sri Yuliawati Sri Yuliawati Sri Yuliawati Sukarman Sukarman Supriyadi Supriyadi Sutra Diyana Tanjung, Puspasari Noerwan Tanjung, Puspasari Noerwan Tatik Handayani, Tatik Theresia Damayanti Tuti Yuniatun, Tuti Wardana, Tegar Pratama Kayong Wibisono, Shafa’ Khairunnisa Wijanarka Wijanarka Wulandari, Sukma Aulia YOPI YOPI Yudanto, Yusuf Arya