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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Berkala Ilmiah Pendidikan Biologi (BioEdu) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Alchemy : Journal of Chemistry Jurnal Harpodon Borneo Biotropika ZOO INDONESIA Jurnal Biodjati Jurnal Abdi: Media Pengabdian Kepada Masyarakat Sains dan Matematika Zoo Indonesia Proceeding Biology Education Conference Borneo Journal of Biology Education (BJBE) Journal of Fisheries & Marine Biosaintifika: Journal of Biology & Biology Education BIO-SAINS | Jurnal Ilmiah Biologi Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Dwi Anggorowati Rahayu
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Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Journal of Fisheries

 1 
Morphological and Molecular Characteristics of Pufferfish Using DNA Barcode COI Gene at Camplong Beach, Madura, Indonesia Adam Dwi Rangga; Viona Angelina Erlan Panjaitan; Endik Deni Nugroho; Rahayu, Dwi Anggorowati; Cahya Ajeng Valentina Sulung; Rusdianto, Rusdianto; Noorhidayah Binti Mamat
Jurnal Ilmiah Perikanan dan Kelautan 2026: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2026)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

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Abstract

Graphical Abstract Highlight Research 1. Combining DNA barcoding with morphological variation analysis can enhance the accuracy of Pufferfish species identification. 2. This is the first study to examine the genetic diversity and phylogenetic relationships pufferfish from Madura, East Java, using partial COI sequences. 3. DNA barcoding confirms the morphological identification of this group (96%-100%) 4. The reliability using targeted DNA barcoding and phenetic taxonomy to study genetic variation and identify species differences in pufferfish.   Abstract Pufferfish are one of the most common fish species in the ocean particularly in Camplong Beach, Madura. Despite the abundant nature, there isn’t much information on this species in East Java, showing the need for identification. Therefore, this study sought to identify pufferfish species in Sampang Regency through morphological and molecular characterization utilizing DNA barcoding of the COI gene. Fins were kept in 96% absolute ethanol, and DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed using bioinformatics tools, including the BOLD system and the web-based Automatic Barcode Gap Discovery (ABGD) tool. To strengthen genetic study, phenetic taxonomy was again applied using Ntysc. Morphological identification revealed five distinct species of pufferfish, namely Chelonodontops patoca, Lagocephalus spadiceus, Arothron reticularis, Otracion rhinorhynchos, and Cyclichthys orbicularis. The high haplotype diversity (Hd) of 0.9 and nucleotide diversity (π) of 0.2 indicated substantial genetic variation. There were 11 polymorphic sites, and the overall ts/tv ratio was 2.2. The frequency of parsimonious informative sites was 21.6%. The phylogenetic tree displayed distinct branching patterns among species. Morphological and molecular identification data supported these results, demonstrating that five distinct pufferfish species occur at Camplong Beach in the Sampang District of Madura.
Co-Authors - Ibrahim, - Abdul Basith Adam Dwi Rangga Afnani, Moh. Royhan Afnani, Royhan Agistiana Azzahra, Athiyya Nur Ahmad Bashri Ahwan, Zainul Ainiyah, Roisatul Aji, Fafit Rahmat Aji, Fatit Rahmar Amang Fathurrohman Amang Fathurrohman, Amang Anissa, Ratih Khairul Arumsari, Maharani Dyah Azrianingzih, Rodiyati Azrianingzih, Rodliyati Cahya Ajeng Valentina Sulung Damayanti, Ayu Elysia Dayat, Muhammad Dwi Listyorini ENDANG SUSANTINI Endik Deni Nugroho Fajriyah, Luluatul Fida Rachmadiarti Hamidah, Safira Aini Ilmi Haryono Haryono Hendrata, Excelino Herlina Fitrihidajati Ibrohim Jamilah, Yaquta Maziyatin Kamila, Rahmadian Kahfi Zulfa Kasiman, Kasiman Kautsaranny, Safira Khaleyla, Firas Khoiriza, Indana Nurul Kurniawati, Yusfita Mamat, Noorhidayah Binti Maramis, Arnetta Yolanda Mudloifah, Istatik Muji Sri Prastiwi Nia Kurniawan Noorhidayah Binti Mamat Novelia, Adelin Nugroho, Hafiidh Haqqu Nur Rohmatin Isnaningsih, Nur Rohmatin Nurdiana, Maulida Nurinsiyah, Ayu Savitri Panjaitan, Viona Angelina Erlan Rabbani, Calista Naya Rangga, Adam Dwi Reni Ambarwati Reza Ardiansyah Rinie Pratiwi Puspitawati Rodiyati Azrianingsih Rofiah Mauludiyah, Nurhidayati Rohmah, Tri Lestari Saidha Roisatul Ainiyah Rupa, Darius Rusdianto Rusdianto, Rusdianto Sonia, Shela Sulung, Cahya Ajeng Valenta Tresna Sunu Kuntjoro Sururi, Ahmad Misbakhus TARZAN PURNOMO Tresna Sulung, Cahya Ajeng Valenta Ulfi Faizah Viona Angelina Erlan Panjaitan Wibisono, Mulyono Wibowo, Dhea Mutiara Fernanda WIDOWATI BUDIJASTUTI Winarsih Winarsih