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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Berkala Ilmiah Pendidikan Biologi (BioEdu) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Alchemy : Journal of Chemistry Jurnal Harpodon Borneo Biotropika ZOO INDONESIA Jurnal Biodjati Jurnal Abdi: Media Pengabdian Kepada Masyarakat Sains dan Matematika Zoo Indonesia Jurnal Teknik Elektro (JTE) Proceeding Biology Education Conference Borneo Journal of Biology Education (BJBE) Journal of Fisheries & Marine Biosaintifika: Journal of Biology & Biology Education BIO-SAINS | Jurnal Ilmiah Biologi Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Dwi Anggorowati Rahayu
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Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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In Silico Study: ACE Inhibitory Activity as a Marine Animal Fatty Acid Antihypertensive Candidate Arumsari, Maharani Dyah; Sururi, Ahmad Misbakhus; Hendrata, Excelino; Kamila, Rahmadian Kahfi Zulfa; Wibowo, Dhea Mutiara Fernanda; Fajriyah, Luluatul; Rahayu, Dwi Anggorowati
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.29899

Abstract

Marine life has much potential for development, especially in the medical field. Its main content is fatty acids, where fatty acids have potential as antihypertensive agents. This research aims to determine the potential of the primary fatty acids in marine biota as antihypertensive agents through an in-silico approach using molecular docking. This study uses the Angiotensin Converting Enzyme (ACE) receptor as the target protein and fatty acid ligands (myristic acid, pentadecanoic acid, eicosapentaenoic acid, linoleic acid, vaccenic acid, 11-eicosenoic acid, palmitic acid), and the control drug captopril for comparison. The initial stages of the research include protein and ligand preparation, followed by molecular docking and visualization. Potential compounds were then analyzed with Lipinski drug-likeness and PASSOnline. The research results show that eicosapentaenoic acid and linoleic acid have the potential as ACE inhibitors. PASSOnline predictions indicate that both had a high probability of being vasodilator agents. Therefore, these two fatty acids had the potential as antihypertensive agents. Further research is needed through in vitro and in vivo testing to utilize marine biota in the medical world.
Morphological and Molecular Characterization of Pufferfish from Lekok Coastal Area, Pasuruan Indonesia Panjaitan, Viona Angelina Erlan; Rangga, Adam Dwi; Sulung, Cahya Ajeng Valenta Tresna; Rahayu, Dwi Anggorowati; Khaleyla, Firas; Nugroho, Endik Deni; Rusdianto, Rusdianto; Mamat, Noorhidayah Binti
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.26663

Abstract

Pufferfish belong to the Tetraodontidae family, which consists of 28 genera and 184 species; however, there are limited studies on the species from East Java, especially Pasuruan. This indicates the need for the collection of more accurate morphological and genetic resources as an important step for its conservation. Therefore, this study was conducted to identify pufferfish species found in the Lekok Coastal Waters, Pasuruan District, through morphological characterization and molecular analysis using DNA barcoding of the COI gene. A total of 12 samples were collected from the Lekok Coastal Waters, Pasuruan Regency. Fin samples were stored in 96% absolute ethanol. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed using bioinformatics tools, the BOLD system, and the Automatic Barcode Gap Discovery (ABGD) web platform. Phenetic taxonomy was further applied using Ntysc to enhance the robustness of genetic analysis. The results showed that there were identified five pufferfish species were identified: Lagocephalus spadiceus, Chelonodontops patoca, Cyclichthys orbicularis, Arothron stellatus, and Arothron reticularis. High haplotype diversity (Hd) of 1 and nucleotide diversity (π) of 0.1 indicate significant genetic variation. The frequency of parsimonious informative sites was 22.6%, with 13 polymorphic sites and an overall ts/tv ratio of 2.2. The phylogenetic tree showed unambiguous branching patterns among species. These findings are supported by morphological and molecular identification results, which indicate the presence of five species of pufferfish along the Lekok Coast, Pasuruan Regency.
Pengembangan Flipbook Berbasis Reciprocal Teaching pada Materi Perubahan Lingkungan untuk Melatihkan Keterampilan Metakognitif Siswa X SMA Nurdiana, Maulida; Rachmadiarti, Fida; Rahayu, Dwi Anggorowati
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol. 13 No. 2 (2024)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/bioedu.v13n2.p383-391

Abstract

Abundance and Potential of Asteroidea as Bioindicator of Lead (Pb) in the Coastal Zone at Bangkalan Regency, Madura Kurniawati, Yusfita; Maramis, Arnetta Yolanda; Mudloifah, Istatik; Rahayu, Dwi Anggorowati
Biotropika: Journal of Tropical Biology Vol. 11 No. 2 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.02.04

Abstract

Asteroidea is a marine invertebrate that has a role as a bioindicator, but the research related to the abundance and potential of Asteroidea as a bioindicator of lead on Madura Island is not to be done. This study aimed to analyse the effect of Pb concentration on Asteroidea population density in Bangkalan Regency. For collecting data, the transect plotting method with a plot measuring 1x1 m2 with 30 plots in the intertidal zone. Seawater sampling was divided into three stations, such as station I-0 meters (edge), station II-50 meters (middle) and station III-100 meters (deep zone), then the seawater samples were tested for lead Pb levels using the AAS method (atomic absorption spectrophotometer). Data analysis was carried out with quantitative descriptive with Asteroidea composition including diversity index, relative density and dominance index as parameters. The results obtained were sea star species, Cryptasterina sp. and Aquilonastra coronata, with a diversity index value of 0, a relative density of 100% and a dominance index of 1. The results for Pb concentration on Tengket Beach at a depth of 0 meters, 50 meters and 100 are 0.072 ppm; 0.16 ppm; 0.61 ppm and Pantai Modung at 0.5 ppm; 0.83 ppm; 0.82 ppm. Based on the analysis of the abundance of Asteroidea, the higher the concentration of Pb contained in the waters, the lower the density of Asteroidea. It was concluded that there was no relationship between Pb concentrations in seawater and sea star population density, found a high lead metal yield on the diversity and abundance of sea stars.
Morphological and Genetic Variation of Filopaludina javanica (von dem Busch, 1844) (Gastropoda: Viviparidae) from Madura Island, Indonesia Nugroho, Hafiidh Haqqu; Ambarwati, Reni; Isnaningsih, Nur Rohmatin; Rahayu, Dwi Anggorowati
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.264-277

Abstract

Filopaludina javanica (von dem Busch, 1844) is a freshwater gastropod species in the Viviparidae family. Filopaludina javanica is widely distributed in freshwater waters in Java (including Madura), Sulawesi, Sumatra, Borneo, Papua, Thailand, and Vietnam. The morphological, morphometric, and molecular characterization studies of F. javanica based on the COI gene originating from Madura Island are still quite limited. Therefore, this study aims to determine the morphological variations, morphometry, and molecular characters of F. javanica in Bangkalan, Madura. The samples used are the Taxonomy Laboratory collection from Madura Island, followed by morphological observations, morphometry, and analysis of DNA: isolation, amplification, electrophoresis, and COI gene sequencing. Morphological and morphometric variations in F. javanica from Madura Island showed seven types of morphological variations. Principal Component Analysis (PCA) scatterplot results showed morphometric clustering of F. javanica based on morphological type. Identification of F. javanica using BLAST and comparison with the GenBank database revealed five nucleotide base variations, with an overall genetic distance of 0.031. Therefore, the phylogenetic tree shows that F. javanica from Madura Island belongs to the same clade as F. javanica from Sarawak, West Java, and North Kalimantan.
MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF Donax faba (BIVALVIA: DONACIDAE) OBTAINED FROM KUTANG BEACH, LAMONGAN, INDONESIA Ambarwati, Reni; Rachmadiarti, Fida; Fitrihidajati, Herlina; Purnomo, Tarzan; Rahayu, Dwi Anggorowati; Faizah, Ulfi
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.2143

Abstract

ARTICLE HIGLIGHTSThis study revealed 19 morphological variations of edible wedge clams, Donax faba (Bivalvia: Donacidae), from Kutang Beach, Lamongan, Indonesia, which has significant role as part of coastal ecosystem.This reseach also highlights the DNA barcoding of Donax faba based on COI gene (Cytochrome C Oxidase Subunit I).The average genetic distance of the research samples was 0.46%, while the value of this parameter between the research samples and the ingroup was found to be 1.51%. ABSTRACTDonax faba is a type of wedge clams with significant ecological and economic roles, as well as variations in color, pattern, and morphometric properties. Recently, a particular population of unidentified D. faba was reported from Kutang Beach, Lamongan, Indonesia. Therefore, this research aimed to assess the morphological variations and molecular characteristics of D. faba obtained from Kutang Beach based on COI gene. A total of 288 samples were collected during the lowest tide for morphological characterization of the color, pattern, and morphometrics of the shells. Additionally, molecular characterization was conducted based on the composition of nucleotide bases and amino acids of COI gene, genetic distance, as well as the relationships. The results showed that D. faba had 19 morphological variations, among which the most dominant type contained a whitish exterior with brown spots and a whitish purple interior. The average genetic distance of the samples was 0.46%, while the value was calculated as 1.51% between the samples and the ingroup. Automatic Barcode Gap Discovery (ABGD) analysis performed using a prior maximal distance of 0.001 showed the separation of these species into distinct categories.
FURTHER STUDY ON TWO SPECIES OF LOACH FISHES (Cypriniformes: Nemacheilidae: Nemacheilus) BASED ON MORPHOLOGY AND MOLECULAR DATA Rahayu, Dwi Anggorowati; Kuntjoro, Sunu; Budijastuti, Widowati; Winarsih, Winarsih; Ambarwati, Reni; Nugroho, Endik Deni; Basith, Abdul; Kurniawan, Nia; Haryono, Haryono
BIOTROPIA Vol. 30 No. 3 (2023): BIOTROPIA Vol. 30 No. 3 December 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1942

Abstract

The identities of two local loaches, Nemacheilus chrysolaimos (Valenciennes, 1846) and N. fasciatus (Valenciennes, 1846) from six rivers, were obtained through a comprehensive examination of their morphology and molecular characteristics in Biltar Regency, East Java, Indonesia. Therefore, this study identified Nemacheilus spp. from Blitar based on morphology and partial sequence of COI. The meristic data obtained for N. chrysolaimos included DII. 7–8 (dorsal fin), AI. 3–5 (anal fin), PI. 9 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). On the other hand, N. fasciatus exhibited the following meristic data, namely D II 7–8 (dorsal fin), AI. 6 (anal fin), PI. 9–10 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). A significant difference was observed in the morphometric characteristics of N. fasciatus across various sampling sites, as determined by the Kruskal-Wallis Test. Furthermore, the nucleotide base composition sequences of Nemacheilus spp. consisted of Thiamine (T), Cytosine (C), Adenine (A), and Guanine (G) with a mean of 29.565%, 32.023%, 23.88%, and 16.244%. Maximum Likelihood (ML) and Minimum Evolution (ME) phylogenetic analysis was also conducted using the Kimura 2 Parameter model to establish two major clades on Nemacheilus spp. and one out-group significantly different from the Nemacheilus spp. The results showed that these major clades exhibited a close relationship at 100% bootstrap support and were grouped under the genus Nemacheilus. The study on Nemacheilus spp. from the Blitar locality differentiated COI sequences between N. fasciatus and N. chrysolaimos. Additionally, N. chrysolaimos, as inferred from reference sequences, was identified as the ancestral species to N. chrysolaimos MZB 26540 and MZB 26539. ABGD analyses, employing a prior maximal distance of 0.025, also indicated the separation of these species into distinct partitions. The integration of morphology and genetic data for Nemacheilus spp. should provide valuable insights for future genetic population studies and conservation initiatives.
Morphological and Molecular Characteristics of Pufferfish Using DNA Barcode COI Gene at Camplong Beach, Madura, Indonesia Adam Dwi Rangga; Viona Angelina Erlan Panjaitan; Endik Deni Nugroho; Rahayu, Dwi Anggorowati; Cahya Ajeng Valentina Sulung; Rusdianto, Rusdianto; Noorhidayah Binti Mamat
Jurnal Ilmiah Perikanan dan Kelautan 2026: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2026)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i2.82924

Abstract

Graphical Abstract Highlight Research 1. Combining DNA barcoding with morphological variation analysis can enhance the accuracy of Pufferfish species identification. 2. This is the first study to examine the genetic diversity and phylogenetic relationships pufferfish from Madura, East Java, using partial COI sequences. 3. DNA barcoding confirms the morphological identification of this group (96%-100%) 4. The reliability using targeted DNA barcoding and phenetic taxonomy to study genetic variation and identify species differences in pufferfish.   Abstract Pufferfish are one of the most common fish species in the ocean particularly in Camplong Beach, Madura. Despite the abundant nature, there isn’t much information on this species in East Java, showing the need for identification. Therefore, this study sought to identify pufferfish species in Sampang Regency through morphological and molecular characterization utilizing DNA barcoding of the COI gene. Fins were kept in 96% absolute ethanol, and DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed using bioinformatics tools, including the BOLD system and the web-based Automatic Barcode Gap Discovery (ABGD) tool. To strengthen genetic study, phenetic taxonomy was again applied using Ntysc. Morphological identification revealed five distinct species of pufferfish, namely Chelonodontops patoca, Lagocephalus spadiceus, Arothron reticularis, Otracion rhinorhynchos, and Cyclichthys orbicularis. The high haplotype diversity (Hd) of 0.9 and nucleotide diversity (π) of 0.2 indicated substantial genetic variation. There were 11 polymorphic sites, and the overall ts/tv ratio was 2.2. The frequency of parsimonious informative sites was 21.6%. The phylogenetic tree displayed distinct branching patterns among species. Morphological and molecular identification data supported these results, demonstrating that five distinct pufferfish species occur at Camplong Beach in the Sampang District of Madura.
RANCANG BANGUN SISTEM MONITORING KETINGGIAN AIR BERBASIS WIRELESS SENSOR NETWORK DI AGROWISATA BANJARSARI JOMBANG Muhammad Hildan Saputra; Alamsyah, Sayyidul Aulia; Rifqi Firmansyah; Rahayu, Dwi Anggorowati
JURNAL TEKNIK ELEKTRO Vol. 15 No. 1 (2026): JANUARI 2026
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jte.v15n1.p13-19

Abstract

Pemantauan ketinggian air di Desa Banjarsari Kecamatan Bandarkedungmulyo Kabupaten Jombang masih dilakukan secara manual, sehingga tidak responsif terhadap perubahan cepat yang merugikan ekosistem perairan. Penelitian ini bertujuan mengembangkan sistem monitoring ketinggian air berbasis Wireless Sensor Network (WSN) dengan komunikasi LoRa. Sistem terdiri dari water level sensor, ESP32, modul LoRa E220-400T22D, dan dashboard web berbasis MQTT, dengan topologi komunikasi point-to-point yang sederhana dan efisien. Hasil pengujian menunjukkan sensor water level mencapai akurasi 96,54% dengan error 3,46%, komunikasi LoRa stabil hingga jarak 147 meter dengan RSSI optimal −34 dBm, dan dashboard menampilkan data real-time dengan latency rata-rata 110–200 ms. Sistem WSN-LoRa ini terbukti feasible dan reliable untuk monitoring ketinggian air real-time dan layak diterapkan sebagai solusi pengelolaan air sawah yang berkelanjutan.
Co-Authors - Ibrahim, - Abdul Basith Adam Dwi Rangga Afnani, Moh. Royhan Afnani, Royhan Agistiana Azzahra, Athiyya Nur Ahmad Bashri Ahwan, Zainul Ainiyah, Roisatul Aji, Fafit Rahmat Aji, Fatit Rahmar Alamsyah, Sayyidul Aulia Amang Fathurrohman Anissa, Ratih Khairul Arumsari, Maharani Dyah Azrianingzih, Rodiyati Azrianingzih, Rodliyati Cahya Ajeng Valentina Sulung Damayanti, Ayu Elysia Dayat, Muhammad Dwi Listyorini ENDANG SUSANTINI Endik Deni Nugroho Fajriyah, Luluatul Fida Rachmadiarti Hamidah, Safira Aini Ilmi Haryono Haryono Hendrata, Excelino Herlina Fitrihidajati Ibrohim Jamilah, Yaquta Maziyatin Kamila, Rahmadian Kahfi Zulfa Kasiman, Kasiman Kautsaranny, Safira Khaleyla, Firas Khoiriza, Indana Nurul Kurniawati, Yusfita Mamat, Noorhidayah Binti Maramis, Arnetta Yolanda Mudloifah, Istatik Muhammad Hildan Saputra Muji Sri Prastiwi Nia Kurniawan Noorhidayah Binti Mamat Novelia, Adelin Nugroho, Hafiidh Haqqu Nur Rohmatin Isnaningsih, Nur Rohmatin Nurdiana, Maulida Nurinsiyah, Ayu Savitri Panjaitan, Viona Angelina Erlan Rabbani, Calista Naya Rangga, Adam Dwi Reni Ambarwati Reza Ardiansyah Rifqi Firmansyah Rinie Pratiwi Puspitawati Rodiyati Azrianingsih Rofiah Mauludiyah, Nurhidayati Rohmah, Tri Lestari Saidha Roisatul Ainiyah Rupa, Darius Rusdianto Rusdianto, Rusdianto Sonia, Shela Sulung, Cahya Ajeng Valenta Tresna Sunu Kuntjoro Sururi, Ahmad Misbakhus TARZAN PURNOMO Tresna Sulung, Cahya Ajeng Valenta Ulfi Faizah Viona Angelina Erlan Panjaitan Wibisono, Mulyono Wibowo, Dhea Mutiara Fernanda WIDOWATI BUDIJASTUTI Winarsih Winarsih