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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Berkala Ilmiah Pendidikan Biologi (BioEdu) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Alchemy : Journal of Chemistry Jurnal Harpodon Borneo Biotropika ZOO INDONESIA Jurnal Biodjati Bioedukasi: Jurnal Pendidikan Biologi Jurnal Abdi: Media Pengabdian Kepada Masyarakat Sains dan Matematika Zoo Indonesia Borneo Journal of Biology Education (BJBE) Rumphius Pattimura Biological Journal Biosaintifika: Journal of Biology & Biology Education BIO-SAINS | Jurnal Ilmiah Biologi Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Dwi Anggorowati Rahayu
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Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Social Sciences Veterinary Other

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Morphological and Molecular Characterization of Pufferfish from Lekok Coastal Area, Pasuruan Indonesia Panjaitan, Viona Angelina Erlan; Rangga, Adam Dwi; Sulung, Cahya Ajeng Valenta Tresna; Rahayu, Dwi Anggorowati; Khaleyla, Firas; Nugroho, Endik Deni; Rusdianto, Rusdianto; Mamat, Noorhidayah Binti
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.26663

Abstract

Pufferfish belong to the Tetraodontidae family, which consists of 28 genera and 184 species; however, there are limited studies on the species from East Java, especially Pasuruan. This indicates the need for the collection of more accurate morphological and genetic resources as an important step for its conservation. Therefore, this study was conducted to identify pufferfish species found in the Lekok Coastal Waters, Pasuruan District, through morphological characterization and molecular analysis using DNA barcoding of the COI gene. A total of 12 samples were collected from the Lekok Coastal Waters, Pasuruan Regency. Fin samples were stored in 96% absolute ethanol. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed using bioinformatics tools, the BOLD system, and the Automatic Barcode Gap Discovery (ABGD) web platform. Phenetic taxonomy was further applied using Ntysc to enhance the robustness of genetic analysis. The results showed that there were identified five pufferfish species were identified: Lagocephalus spadiceus, Chelonodontops patoca, Cyclichthys orbicularis, Arothron stellatus, and Arothron reticularis. High haplotype diversity (Hd) of 1 and nucleotide diversity (π) of 0.1 indicate significant genetic variation. The frequency of parsimonious informative sites was 22.6%, with 13 polymorphic sites and an overall ts/tv ratio of 2.2. The phylogenetic tree showed unambiguous branching patterns among species. These findings are supported by morphological and molecular identification results, which indicate the presence of five species of pufferfish along the Lekok Coast, Pasuruan Regency.
Pengembangan Flipbook Berbasis Reciprocal Teaching pada Materi Perubahan Lingkungan untuk Melatihkan Keterampilan Metakognitif Siswa X SMA Nurdiana, Maulida; Rachmadiarti, Fida; Rahayu, Dwi Anggorowati
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol. 13 No. 2 (2024)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/bioedu.v13n2.p383-391

Abstract

Abundance and Potential of Asteroidea as Bioindicator of Lead (Pb) in the Coastal Zone at Bangkalan Regency, Madura Kurniawati, Yusfita; Maramis, Arnetta Yolanda; Mudloifah, Istatik; Rahayu, Dwi Anggorowati
Biotropika: Journal of Tropical Biology Vol. 11 No. 2 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.02.04

Abstract

Asteroidea is a marine invertebrate that has a role as a bioindicator, but the research related to the abundance and potential of Asteroidea as a bioindicator of lead on Madura Island is not to be done. This study aimed to analyse the effect of Pb concentration on Asteroidea population density in Bangkalan Regency. For collecting data, the transect plotting method with a plot measuring 1x1 m2 with 30 plots in the intertidal zone. Seawater sampling was divided into three stations, such as station I-0 meters (edge), station II-50 meters (middle) and station III-100 meters (deep zone), then the seawater samples were tested for lead Pb levels using the AAS method (atomic absorption spectrophotometer). Data analysis was carried out with quantitative descriptive with Asteroidea composition including diversity index, relative density and dominance index as parameters. The results obtained were sea star species, Cryptasterina sp. and Aquilonastra coronata, with a diversity index value of 0, a relative density of 100% and a dominance index of 1. The results for Pb concentration on Tengket Beach at a depth of 0 meters, 50 meters and 100 are 0.072 ppm; 0.16 ppm; 0.61 ppm and Pantai Modung at 0.5 ppm; 0.83 ppm; 0.82 ppm. Based on the analysis of the abundance of Asteroidea, the higher the concentration of Pb contained in the waters, the lower the density of Asteroidea. It was concluded that there was no relationship between Pb concentrations in seawater and sea star population density, found a high lead metal yield on the diversity and abundance of sea stars.
Morphological and Genetic Variation of Filopaludina javanica (von dem Busch, 1844) (Gastropoda: Viviparidae) from Madura Island, Indonesia Nugroho, Hafiidh Haqqu; Ambarwati, Reni; Isnaningsih, Nur Rohmatin; Rahayu, Dwi Anggorowati
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.264-277

Abstract

Filopaludina javanica (von dem Busch, 1844) is a freshwater gastropod species in the Viviparidae family. Filopaludina javanica is widely distributed in freshwater waters in Java (including Madura), Sulawesi, Sumatra, Borneo, Papua, Thailand, and Vietnam. The morphological, morphometric, and molecular characterization studies of F. javanica based on the COI gene originating from Madura Island are still quite limited. Therefore, this study aims to determine the morphological variations, morphometry, and molecular characters of F. javanica in Bangkalan, Madura. The samples used are the Taxonomy Laboratory collection from Madura Island, followed by morphological observations, morphometry, and analysis of DNA: isolation, amplification, electrophoresis, and COI gene sequencing. Morphological and morphometric variations in F. javanica from Madura Island showed seven types of morphological variations. Principal Component Analysis (PCA) scatterplot results showed morphometric clustering of F. javanica based on morphological type. Identification of F. javanica using BLAST and comparison with the GenBank database revealed five nucleotide base variations, with an overall genetic distance of 0.031. Therefore, the phylogenetic tree shows that F. javanica from Madura Island belongs to the same clade as F. javanica from Sarawak, West Java, and North Kalimantan.
MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF Donax faba (BIVALVIA: DONACIDAE) OBTAINED FROM KUTANG BEACH, LAMONGAN, INDONESIA Ambarwati, Reni; Rachmadiarti, Fida; Fitrihidajati, Herlina; Purnomo, Tarzan; Rahayu, Dwi Anggorowati; Faizah, Ulfi
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.2143

Abstract

ARTICLE HIGLIGHTSThis study revealed 19 morphological variations of edible wedge clams, Donax faba (Bivalvia: Donacidae), from Kutang Beach, Lamongan, Indonesia, which has significant role as part of coastal ecosystem.This reseach also highlights the DNA barcoding of Donax faba based on COI gene (Cytochrome C Oxidase Subunit I).The average genetic distance of the research samples was 0.46%, while the value of this parameter between the research samples and the ingroup was found to be 1.51%. ABSTRACTDonax faba is a type of wedge clams with significant ecological and economic roles, as well as variations in color, pattern, and morphometric properties. Recently, a particular population of unidentified D. faba was reported from Kutang Beach, Lamongan, Indonesia. Therefore, this research aimed to assess the morphological variations and molecular characteristics of D. faba obtained from Kutang Beach based on COI gene. A total of 288 samples were collected during the lowest tide for morphological characterization of the color, pattern, and morphometrics of the shells. Additionally, molecular characterization was conducted based on the composition of nucleotide bases and amino acids of COI gene, genetic distance, as well as the relationships. The results showed that D. faba had 19 morphological variations, among which the most dominant type contained a whitish exterior with brown spots and a whitish purple interior. The average genetic distance of the samples was 0.46%, while the value was calculated as 1.51% between the samples and the ingroup. Automatic Barcode Gap Discovery (ABGD) analysis performed using a prior maximal distance of 0.001 showed the separation of these species into distinct categories.
FURTHER STUDY ON TWO SPECIES OF LOACH FISHES (Cypriniformes: Nemacheilidae: Nemacheilus) BASED ON MORPHOLOGY AND MOLECULAR DATA Rahayu, Dwi Anggorowati; Kuntjoro, Sunu; Budijastuti, Widowati; Winarsih, Winarsih; Ambarwati, Reni; Nugroho, Endik Deni; Basith, Abdul; Kurniawan, Nia; Haryono, Haryono
BIOTROPIA Vol. 30 No. 3 (2023): BIOTROPIA Vol. 30 No. 3 December 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1942

Abstract

The identities of two local loaches, Nemacheilus chrysolaimos (Valenciennes, 1846) and N. fasciatus (Valenciennes, 1846) from six rivers, were obtained through a comprehensive examination of their morphology and molecular characteristics in Biltar Regency, East Java, Indonesia. Therefore, this study identified Nemacheilus spp. from Blitar based on morphology and partial sequence of COI. The meristic data obtained for N. chrysolaimos included DII. 7–8 (dorsal fin), AI. 3–5 (anal fin), PI. 9 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). On the other hand, N. fasciatus exhibited the following meristic data, namely D II 7–8 (dorsal fin), AI. 6 (anal fin), PI. 9–10 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). A significant difference was observed in the morphometric characteristics of N. fasciatus across various sampling sites, as determined by the Kruskal-Wallis Test. Furthermore, the nucleotide base composition sequences of Nemacheilus spp. consisted of Thiamine (T), Cytosine (C), Adenine (A), and Guanine (G) with a mean of 29.565%, 32.023%, 23.88%, and 16.244%. Maximum Likelihood (ML) and Minimum Evolution (ME) phylogenetic analysis was also conducted using the Kimura 2 Parameter model to establish two major clades on Nemacheilus spp. and one out-group significantly different from the Nemacheilus spp. The results showed that these major clades exhibited a close relationship at 100% bootstrap support and were grouped under the genus Nemacheilus. The study on Nemacheilus spp. from the Blitar locality differentiated COI sequences between N. fasciatus and N. chrysolaimos. Additionally, N. chrysolaimos, as inferred from reference sequences, was identified as the ancestral species to N. chrysolaimos MZB 26540 and MZB 26539. ABGD analyses, employing a prior maximal distance of 0.025, also indicated the separation of these species into distinct partitions. The integration of morphology and genetic data for Nemacheilus spp. should provide valuable insights for future genetic population studies and conservation initiatives.
Gastropods Diversity in the Mangrove Ecosystem of Kalanganyar River, Kalianyar Village, Bangil, Pasuruan Kautsar, Ghinahilmi Najalin; Ambarwati, Reni; Rahayu, Dwi Anggorowati
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p071-078

Abstract

Mangrove ecosystems are very important habitats for various organisms, including gastropods, which function as primary decomposers in the ecological cycle. This study aims to identify the diversity of gastropod species in the mangrove area along the Kalanganyar River, Kalianyar Village, Bangil, Pasuruan. Samples were collected randomly around mangrove roots, followed by morphological identification and species classification. The results showed that there were five gastropod species from four families, namely Pirenella cingulata, Telescopium telescopium, Sermyla riquetii, Neripteron violaceum, and Ellobium sp. The Shannon-Wiener diversity index (H') of 1.165 indicates a moderate level of diversity, with Pirenella cingulata as the most dominant species. Water quality tests showed that copper (Cu) levels were still below the threshold, but lead (Pb) levels exceeded seawater quality standards. These results indicate that the mangrove ecosystem is fairly stable, although there are environmental pressures that need to be addressed, particularly those related to heavy metal pollution.
Pendekatan Fenetik Taksonomi dalam Identifikasi Kekerabatan dan Pengelompokkan Ikan Genus Tor di Indonesia RAHAYU, DWI ANGGOROWATI; NUGROHO, ENDIK DENI
Bioedukasi: Jurnal Pendidikan Biologi Vol 7, No 1 (2014): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v7i1.2844

Abstract

Ikan Tor merupakan ikan air tawar lokal Indonesia dan termasuk ikan terancam punah. Upaya konservasi yang harus dilakukan salah satunya adalah penentuan kekerabatan berdasarkan pendekatan fenetik. Hal ini dikarenakan kedudukan taksonomi dan klasifikasi diantara genus Tor masih rancu. Sumber data yang digunakan adalah karakter meristrik, morfometrik, morfologi secara umum, dan karakter khusus penentu spesies. Karakter khusus penentu spesies diantara ikan genus Tor adalah keberadaan dan ukuran cuping pada bibir bawah. Sampel ikan Tor diambil dari Telaga Banyu Biru Pasuruan (nama lokal ikan Sengkaring dan Tambra), serta spesies acuan (Tor tambraides dari Kalimantan Barat, Tor duoronensis dari Padang, and Tor soro dari Sumatra Utara). Fenogram dibentuk berdasarkan metode UPGMA. Berdasarkan fenogram yang terbentuk diperoleh 3 kelompok kekerabatan dari yang terdekat hingga terjauh dan 2 kelompok apomorfi serta 4 kelompok automorfi. Percabangan pertama terdiri dari dua subklad yaitu Tor duoronensis dan Sengkaring memiliki kesamaan 100%, dan Tambra merupakan sister clad dari Tor duoronensis yang didukung dengan nilai similaritas 92,9%. Subklad kedua terdiri dari Tor Tambra dan Tor soro (Apomorfi B) dengan nilai similaritas 59%, sedangkan Tor tambraides memiliki kekerabatan terjauh dengan indeks kesamaan sebesar 45, 625%. Ikan Sengkaring dan Tambra menunjukkan pola pengelompokan yang dekat dengan Tor duoronensis berdasarkan tujuh karakter morfometrik pembeda utama. Ketujuh karakter pembeda utama tersebut adalah ukuran cuping, SL (panjang standart), MXBL (panjang sungut rahang atas), CPL (panjang batang ekor), PDL (panjang sebelum sirip dorsal), IW (jarak antar mata) dan SNL (panjang moncong). Hasil analisis morfometrik menunjukkan bahwa ikan Sengkaring dan Tambra mutlak bukan merupakan ikan Tor Tambraides maupun Tor soro dikarenakan ukuran tubuh yang berbeda secara signifikan.
Co-Authors - Ibrahim, - Abdul Basith Afnani, Moh. Royhan Afnani, Royhan Agistiana Azzahra, Athiyya Nur Ahmad Bashri Ahwan, Zainul Ainiyah, Roisatul Aji, Fafit Rahmat Aji, Fatit Rahmar Amang Fathurrohman Anissa, Ratih Khairul Arumsari, Maharani Dyah Azrianingzih, Rodiyati Azrianingzih, Rodliyati Damayanti, Ayu Elysia Dayat, Muhammad Dwi Listyorini ENDANG SUSANTINI Endik Deni Nugroho Fajriyah, Luluatul Fida Rachmadiarti Hamidah, Safira Aini Ilmi Haryono Haryono Hendrata, Excelino Herlina Fitrihidajati Ibrohim Jamilah, Yaquta Maziyatin Kamila, Rahmadian Kahfi Zulfa Kasiman, Kasiman Kautsar, Ghinahilmi Najalin Kautsaranny, Safira Khaleyla, Firas Khoiriza, Indana Nurul Kurniawati, Yusfita Mamat, Noorhidayah Binti Maramis, Arnetta Yolanda Mudloifah, Istatik Muji Sri Prastiwi Nia Kurniawan Novelia, Adelin Nugroho, Hafiidh Haqqu Nur Rohmatin Isnaningsih, Nur Rohmatin Nurdiana, Maulida Nurinsiyah, Ayu Savitri Panjaitan, Viona Angelina Erlan Rabbani, Calista Naya Rangga, Adam Dwi Reni Ambarwati Reza Ardiansyah Rinie Pratiwi Puspitawati Rodiyati Azrianingsih Rofiah Mauludiyah, Nurhidayati Rohmah, Tri Lestari Saidha Roisatul Ainiyah Rupa, Darius Rusdianto Rusdianto, Rusdianto Sonia, Shela Sulung, Cahya Ajeng Valenta Tresna Sunu Kuntjoro Sururi, Ahmad Misbakhus TARZAN PURNOMO Tresna Sulung, Cahya Ajeng Valenta Ulfi Faizah Wibisono, Mulyono Wibowo, Dhea Mutiara Fernanda WIDOWATI BUDIJASTUTI Winarsih Winarsih