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Isolation And Characterization of Lactobacillus Species From Local Indonesian Cow’s Milk Kandou, Abigail Sabrina; Sugata, Marcelia; Jo, Juandy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7792

Probiotics are a group of beneficial microorganisms that can improve the health of their host. Lactobacillus species are lactic acid bacteria that have great potential as probiotics, in which cow’s milk is a great source of lactic acid bacteria. In this study, Lactobacillus spp. were isolated from fresh local Indonesian cow’s milk. The methods used to characterize the Lactobacillus spp. were (i) biochemical tests including catalase, hemolytic and sugar fermentation tests; (ii) tolerance tests against salt (NaCl 2%, 4% and 6%), low pH (2, 3, 4, 5, 6 and 7) and temperature (7°C, 37°C and 45°C); and (iii) 16S rRNA sequencing. The isolation yielded 14 isolates matching the criteria of Lactobacillus spp. colony and cell (i.e., Gram positive rods that did not produce endospores and did not have a waxy layer covering its cell wall). Based on subsequent biochemical tests, 5 isolates were suspected as potential probiotic Lactobacillus spp. The 16S rRNA sequencing revealed that the isolate L was Limosilactobacillus fermentum. In conclusion, this study demonstrated that local cow’s milk could be used to isolate Lactobacillus spp.

A Comparative Efficacy of Atezolizumab plus Bevacizumab Versus Sorafenib in Advanced Hepatocellular Carcinoma: A Review Marbun, Kristin Talia; Jo, Juandy
Medicinus Vol 12, No 1 (2022): October
Publisher : Fakultas Kedokteran Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/med.v10i2.7023

Hepatocellular carcinoma (HCC) ranks sixth as the most common cancer and fourth as the most common cause of cancer-related death globally. The standard treatment for advanced HCC is by prescribing sorafenib, a tyrosine kinase inhibitor. Despite its moderate efficacy and concerning side effects, there is no better alternative to sorafenib to treat HCC. However, a new combination of atezolizumab (an inhibitor of PD-L1) and bevacizumab (an inhibitor of vascular endothelial growth factor), has shown a potential to surpass the efficacy of sorafenib. This review was written to provide an insight into pharmacodynamics of sorafenib and atezolizumab plus bevacizumab, effectiveness of sorafenib and the one of atezolizumab plus bevacizumab, utilization of atezolizumab plus bevacizumab in the clinical practice, as well as to argue that this combination can replace sorafenib as the standard palliative treatment for HCC.

RNAi-Based Therapy: Prospect as Cancer Treatment Soentoro, Samuel E; Timothy, Michael; Jo, Juandy
Medicinus Vol 13, No 2 (2024): February
Publisher : Fakultas Kedokteran Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/med.v13i2.8110

Cancer is one of the deadliest form of diseases in humans, with the annual deaths ranging in the millions. Conventional treatments including chemotherapy, radiotherapy, or surgery has their limitations, including common off-target and non-specific effects. Ribonucleic acid interference (RNAi) offers a new strategy for treating cancer by silencing specific genes to prevent gene expression. This review highlights the application of RNAi-based approach in targeting cancer, discusses its potential advantages and limitations, summarizes the existing clinical trials and provides a greater understanding of RNAi-based therapy in cancer.

Understanding Tsetse Fly (Glossina morsitans) Behavior through its Genome Marbun, Kristin Talia; Nugroho, Satya; Jo, Juandy
Medicinus Vol 12, No 2 (2023): February
Publisher : Fakultas Kedokteran Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/med.v10i3.7036

Glossina morsitans (G. morsitans), commonly known as tsetse fly, have caused public health concerns throughout the years. G. morsitans is the vector for Trypanosoma brucei (T. brucei), the parasite responsible for causing the deadly African sleeping disease (African trypanosomiasis). Researchers have searched for ways to contain this disease, but to little avail. Fortunately, new advances in sequencing methods have given researchers a new opportunity to win the war against the disease. The whole-genome sequence of G. morsitans provides essential data regarding involved genes that transmits T. brucei to humans. Information about those unique genes facilitates researchers to create new methods to prevent G. morsitans from becoming the vector of T. brucei, enabling the containment of this disease. With this, we review the unique genes in the G. morsitans genome, such as those that contribute to blood-feeding ability, establish a relationship with symbionts, and G. morsitans unique sensory genes, with an expectation that it would enhance our knowledge of G. morsitans as the vector for parasites causing African trypanosomiasis.

Future Application of Oncolytic Viruses for Cancer Treatment Lesmana, Jevon Aaron; Jo, Juandy
Medicinus Vol 12, No 2 (2023): February
Publisher : Fakultas Kedokteran Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/med.v10i3.7037

Cancer treatments have developed over the years. A particular improvement is the utilization of oncolytic viruses to treat cancers. Oncolytic viruses are one of the immunotherapeutic tools that potentially could provide good results and benefits to the patients. Oncolytic viruses could mediate antitumor effects. Indeed, the connection between viral infections and cancer treatment have been reported historically. It is known that oncolytic viruses prefer to infect cancer cells rather than normal cells, resulting in the presentation of tumor-associated antigens to the immune system, boosting immunological activity in the tumor microenvironment, as well as assisting in the expression of inflammatory and immunomodulatory cytokines. Oncolytic viruses are a novel regimen in the cancer therapy, in which knowledge and technology of utilizing oncolytic viruses to treat cancer are still evolving. Importantly, clinical trials demonstrated that the viruses were well tolerated by cancer patients. Considering its potency and prospect, oncolytic viral treatments could be a useful additional tool for cancer therapy.

Safety Assessment of Bacillus subtilis G8 Isolated from Natto for Food Application Putri, Nathania Calista; Victor, Hans; Litanto, Vivian; Pinontoan, Reinhard; Jo, Juandy
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87079

Various bacteria are widely used as food-fermenting agents, including Lactobacillus, Bifidobacterium, and Bacillus. Despite they are generally recognized as safe to be consumed by humans, those bacteria could potentially cause antibiotic resistance as they could acquire and transfer antibiotic resistance genes from or to other microbes within the human gastrointestinal tract. Profiling antibiotic resistance pattern in those bacteria is therefore important to control the spread of antibiotic resistance. In this study, antibiotic resistance profile of Bacillus subtilis G8 was assessed. B. subtilis G8 had been isolated from commercialised Japanese natto in Indonesia and had been previously reported for its fibrinolytic characteristics. The antibiotic resistance phenotype and genotype of B. subtilis G8 were assessed through the Kirby-Bauer disk diffusion method and whole-genome analysis, respectively. B. subtilis G8 exhibited resistance towards Oxacillin, Lincomycin and Tiamulin-Lefamulin. The bioinformatics analysis indicated several responsible genes mediating those resistance, i.e., ybxI (for Oxacillin), lmrB (for Lincomycin) and vmlR (for Lincomycin and Tiamulin-Lefamulin). All identified genes were found in the chromosomal DNA. Further analysis found no mobile genetic elements within the genome, therefore reducing a risk of resistance gene transfer via plasmid and subsequently supporting safety profile of B. subtilis G8 in food fermentation usage.

WORKSHOP INTERAKTIF TERKAIT PENYAKIT COVID-19 DAN VARIANNYA BAGI PARA SISWA SEKOLAH MENENGAH ATAS [INTERACTIVE WORKSHOP RELATED TO COVID-19 AND ITS VARIANTS FOR HIGH SCHOOL STUDENTS] Sugata, Marcelia; Jo, Juandy; Soewono, Effendi; Pinontoan, Reinhard; Litanto, Vivian; Victor, Hans; Abigail Christy, Suawa Natania; Vidian, Valerie; Sophiano, Theophilus; Febriani, Enjelia; Jan, Tan Tjie
Jurnal Sinergitas PKM & CSR Vol 6, No 2 (2022): OCTOBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jspc.v6i2.5708

An interactive workshop related to COVID-19 and its variants was held during the introduction of Biology Study Program, Pelita Harapan University to high school students. This activity was carried out online and was divided into two sessions. The first session was socialization about COVID-19 and its variants, while the second session was the time to play an online game called ATtack Covid Variant (ATCV). The success of this activity was measured based on the surveys given before and after the activity. The survey results showed that socialization and online game ATCV have succeeded in increasing students' knowledge and understanding of COVID-19 and its variants. In addition, the students were satisfied with the ATCV game because of the benefits obtained from this game, the rules are easy to understand, and the visuals of the game are attractive.Bahasa Abstract:Workshop interaktif terkait COVID-19 dan variannya dilaksanakan saat kegiatan perkenalan Program Studi Biologi, Universitas Pelita Harapan kepada siswa/i sekolah menengah. Dalam pelaksanaannya, kegiatan ini dilakukan secara daring dan dibagi menjadi dua sesi. Sesi pertama adalah sosialisasi tentang COVID-19 dan variannya, sedangkan sesi kedua merupakan waktu untuk melakukan permainan daring yang bernama ATtack Covid Variant (ATCV). Keberhasilan kegiatan ini diukur berdasarkan survei yang diberikan sebelum dan setelah kegiatan. Hasil survei menunjukkan bahwa kegiatan sosialisasi dan permainan daring ATCV berhasil meningkatkan pengetahuan dan pemahaman para siswa mengenai COVID-19 dan variannya. Selain itu, para siswa merasa puas dengan permainan ATCV karena manfaat yang diperoleh dari permainan ini, peraturan yang mudah dipahami, serta visual permainan yang menarik.

Evaluation of anti-diphtheria toxoid antibody persistence in school-age children in Jakarta, Indonesia Santi, Theresia; Prayitno, Ari; Munasir, Zakiudin; Hadinegoro, Sri Rezeki S.; Harahap, Alida Roswita; Werdhani, Retno Asti; Sah Bandar, Ivo Novita; Jo, Juandy; Hegar, Badriul
Paediatrica Indonesiana Vol. 64 No. 5 (2024): September 2024
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/pi64.5.2024.447-53

Background Diphtheria can be effectively prevented by adequate immunization. A combined vaccine against diphtheria toxoid, pertussis, and tetanus toxoid (DPT) is currently used in routine pediatric immunizations. Outbreaks of diphtheria could emerge in Indonesia as a consequence of declining routine vaccination during the COVID-19 pandemic. Objective To analyze the impact of the first (administered at 18-24 months of age ) and second diphtheria boosters (administered at 5-7 years of age ) in retaining protective levels of anti-diphtheria toxoid antibodies. We also investigated for relevant factors associated with anti-diphtheria toxoid antibody titers. Methods This cross-sectional study was conducted in the Senen District of Jakarta, Indonesia. The inclusion criteria were healthy children aged 6 to 7 years with documented history of DPT vaccination. Primary vaccination defined as 3 doses of DPT at age less than 1 year , first booster was DPT vaccination at 18-24 years of age, and second booster was diphtheria-tetanus (DT) vaccination received at 5 to 7 years of age. Peripheral blood specimens were obtained from participating children, after informed consent was provided by their parents. Antibodies against diphtheria in sera specimens were assessed by commercial anti-diphtheria toxoid immunoglobulin G (IgG) enzyme-linked immunosorbent assay. Results There were 154 children included in the study, with a female majority (61%). Overall, specific humoral immunity against diphtheria was observed in 113 children (73.4%). There was no statistical difference in immunity level between genders. Importantly, children who received the first and second diphtheria booster had significantly higher anti-diphtheria antibody level than those who did not receive both diphtheria booster (P<0.001). Conclusion Booster vaccinations are crucial among school-age children in Indonesia to improve their anti-diphtheria immunity and to minimize a risk of diphtheria outbreaks.

WORKSHOP INTERAKTIF TERKAIT PENYAKIT COVID-19 DAN VARIANNYA BAGI PARA SISWA SEKOLAH MENENGAH ATAS [INTERACTIVE WORKSHOP RELATED TO COVID-19 AND ITS VARIANTS FOR HIGH SCHOOL STUDENTS] Sugata, Marcelia; Jo, Juandy; Soewono, Effendi; Pinontoan, Reinhard; Litanto, Vivian; Victor, Hans; Abigail Christy, Suawa Natania; Vidian, Valerie; Sophiano, Theophilus; Febriani, Enjelia; Jan, Tan Tjie
Jurnal Sinergitas PKM & CSR Vol. 6 No. 2 (2022): OCTOBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jspc.v6i2.5708

An interactive workshop related to COVID-19 and its variants was held during the introduction of Biology Study Program, Pelita Harapan University to high school students. This activity was carried out online and was divided into two sessions. The first session was socialization about COVID-19 and its variants, while the second session was the time to play an online game called ATtack Covid Variant (ATCV). The success of this activity was measured based on the surveys given before and after the activity. The survey results showed that socialization and online game ATCV have succeeded in increasing students' knowledge and understanding of COVID-19 and its variants. In addition, the students were satisfied with the ATCV game because of the benefits obtained from this game, the rules are easy to understand, and the visuals of the game are attractive.Bahasa Abstract:Workshop interaktif terkait COVID-19 dan variannya dilaksanakan saat kegiatan perkenalan Program Studi Biologi, Universitas Pelita Harapan kepada siswa/i sekolah menengah. Dalam pelaksanaannya, kegiatan ini dilakukan secara daring dan dibagi menjadi dua sesi. Sesi pertama adalah sosialisasi tentang COVID-19 dan variannya, sedangkan sesi kedua merupakan waktu untuk melakukan permainan daring yang bernama ATtack Covid Variant (ATCV). Keberhasilan kegiatan ini diukur berdasarkan survei yang diberikan sebelum dan setelah kegiatan. Hasil survei menunjukkan bahwa kegiatan sosialisasi dan permainan daring ATCV berhasil meningkatkan pengetahuan dan pemahaman para siswa mengenai COVID-19 dan variannya. Selain itu, para siswa merasa puas dengan permainan ATCV karena manfaat yang diperoleh dari permainan ini, peraturan yang mudah dipahami, serta visual permainan yang menarik.

PENYULUHAN KEBERSIHAN DAN KESEHATAN BAGI SISWA SEKOLAH DASAR NEGERI KELAPA DUA II DI TANGERANG, BANTEN Jo, Juandy; Sugata, Marcelia; Victor, Hans; Jan, Tan Tjie; Pinontoan, Reinhard; Samantha, Ariela; Litanto, Vivian; Christy, Suawa Natania Abigail; Simamora, Chelsea Valeria; Firmansyah, Daniel Elia; Thanos, Celeste Abigail
Jurnal Sinergitas PKM & CSR Vol. 7 No. 3 (2023): DECEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jspc.v7i3.7666

Kebersihan dan kesehatan diri merupakan isu penting di kalangan anak-anak usia 6-12 tahun atau jenjang sekolah dasar (SD) di Indonesia. Umumnya, sekolah telah mengajarkan pentingnya kebersihan diri dan kesehatan, tetapi bahaya nyata yang dapat timbul akibat kelalaian dalam menjaga kebersihan serta kesehatan belum tentu dipahami oleh para siswa SD. Kelalaian menjaga kebersihan dan kesehatan diri dengan baik, dapat mengakibatkan timbulnya penyakit yang dapat mempengaruhi kesehatan pada anak. Dengan begitu, perlu dilakukan penyuluhan mengenai kebersihan dan kesehatan diri untuk mengurangi resiko terkena penyakit. Penyuluhan kebersihan dan kesehatan dilakukan untuk meningkatkan praktik kebersihan diri dan kesehatan pada siswa/i SD negeri Kelapa Dua II Tangerang. Kegiatan penyuluhan ini dilakukan dengan penjelasan dan pemaparan materi yang interaktif seperti video animasi serta gambar-gambar animasi dengan tujuan untuk membuat siswa/i mudah memahami materi yang disampaikan. Pada penyuluhan ini juga dilakukan survei pada sebelum (pre-test) dan setelah (post-test) pemaparan materi untuk melihat pemahaman siswa/i. Dari survei yang telah dilakukan, terlihat adanya peningkatan pemahaman siswa/i mengenai kebersihan dan kesehatan diri. Hal ini terlihat dari peningkatan jumlah reponden (dari 49,2% saat pre-test menjadi 56,4% pada post-test) yang menjawab cara yang dapat dilakukan untuk memiliki hidup sehat adalah dengan makan makanan yang bergizi dan menjaga kebersihan. Oleh karena itu, acara ini dapat dikatakan berhasil meningkatkan pemahaman siswa/i mengenai kebersihan dan kesehatan diri.

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