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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Journal of Tropical Biodiversity and Biotechnology Paediatrica Indonesiana Jurnal Sinergitas PkM & CSR Medicinus : Jurnal Kedokteran FaST : Jurnal Sains dan Teknologi J-HESTECH (Journal Of Health Educational Science And Technology) Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute Unnes Journal of Public Health
Juandy Jo
Universitas Pelita Harapan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Isolation And Characterization of Lactobacillus Species From Local Indonesian Cow’s Milk Kandou, Abigail Sabrina; Sugata, Marcelia; Jo, Juandy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7792

Abstract

Probiotics are a group of beneficial microorganisms that can improve the health of their host. Lactobacillus species are lactic acid bacteria that have great potential as probiotics, in which cow’s milk is a great source of lactic acid bacteria. In this study, Lactobacillus spp. were isolated from fresh local Indonesian cow’s milk. The methods used to characterize the Lactobacillus spp. were (i) biochemical tests including catalase, hemolytic and sugar fermentation tests; (ii) tolerance tests against salt (NaCl 2%, 4% and 6%), low pH (2, 3, 4, 5, 6 and 7) and temperature (7°C, 37°C and 45°C); and (iii) 16S rRNA sequencing. The isolation yielded 14 isolates matching the criteria of Lactobacillus spp. colony and cell (i.e., Gram positive rods that did not produce endospores and did not have a waxy layer covering its cell wall). Based on subsequent biochemical tests, 5 isolates were suspected as potential probiotic Lactobacillus spp. The 16S rRNA sequencing revealed that the isolate L was Limosilactobacillus fermentum. In conclusion, this study demonstrated that local cow’s milk could be used to isolate Lactobacillus spp.
Assessment on the Antimicrobial Activity of Lactiplantibacillus plantarum SU-KC1a against Listeria monocytogenes ATCC-7644 Timothy, Michael; Samantha, Ariela; Sugata, Marcelia; Tan, Tjie Jan; Jo, Juandy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.8038

Abstract

Lactobacillus species are particularly noteworthy due to their ability to synthesize a variety of antimicrobial substances, including organic acids and bacteriocins. Lactiplantibacillus plantarum SU-KC1a, a member of the genus of Lactobacillus, had been previously isolated from human breast milk by our group. A preliminary in silico investigation had identified that Lpb. plantarum SU-KC1a possessed genes responsible for plantaricin, which is a type of bacteriocin produced by Lpb. plantarum strains. This study therefore aimed to assess the antibacterial activity of Lpb. plantarum SU-KC1a against pathogenic Listeria monocytogenes ATCC-7644 and to induce the biosynthesis of plantaricin by Lpb. plantarum SU-KC1a through co-culture with L. monocytogenes ATCC-7644 as the inducer strain. Our results showed that the cell-free supernatants from both co-culture and monoculture of Lpb. plantarum SU-KC1a exhibited a modest antibacterial activity against L. monocytogenes ATCC-7644. However, it was further determined that the inhibitory effect was not due to the production of plantaricin. Instead, it was primary attributed to the production of organic acids, which decreased the pH and inhibited the growth of L. monocytogenes ATCC-7644. In conclusion, our study demonstrated that Lpb. plantarum SU-KC1a could exert a modest antibacterial activity towards L. monocytogenes ATCC-7644, but not through the induction of plantaricin.
PENGUJIAN AKTIVITAS ANTIJAMUR Bacillus amyloliquefaciens STRAIN N1 [ASSESSMENT ON ANTIFUNGAL ACTIVITY OF Bacillus amyloliquefaciens STRAIN N1] Jo, Juandy; Subroto, Sara Thalia; Victor, Hans; Sanjaya, Astia
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 5 No. 1 (2021): NOVEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The negative impact of fungal contamination in food products is profound. The contamination could be caused by fungi that threatens human health as well. Effective and safe antifungal compounds therefore are crucial to control this issue. It had been reported that certain strains of Bacillus amyloliquefaciens exerted an antifungal activity. As a strain of Bacillus amyloliquefaciens (called strain N1) had been isolated by our laboratory from a chicken gizzard, we investigated whether this strain had an antifungal activity as well. Two species of fungi, i.e., Aspergillus welwitschiae and Penicillium sp., were utilized as the target in the whole-cell co-culture method. The result showed that Bacillus amyloliquefaciens strain N1 significantly inhibited the growth of Aspergillus welwitschiae and Penicillium sp. (percentages of inhibition were 73.3% and 100%, respectively). Next, with the availability of whole genome data of Bacillus amyloliquefaciens strain N1, the bioinformatic analyses with RAST and nucleotide BLAST database suggested that Bacillus amyloliquefaciens strain N1 could secrete antifungal compounds of bacillomycin D, surfactin and fengycin. In conclusion, Bacillus amyloliquefaciens strain N1 has a potential to be further utilized due to the presence of its antifungal activity. BAHASA INDONESIA ABSTRAK:Dewasa ini, permasalahan kontaminasi pada produk makanan banyak ditemukan. Kontaminasi ini dapat disebabkan, salah satunya oleh jamur yang dapat mengancam kesehatan manusia. Oleh karena itu, senyawa antijamur yang efektif dan aman dibutuhkan untuk mengendalikan permasalahan ini. Berdasarkan penelitian terdahulu, strain tertentu dari bakteri Bacillus amyloliquefaciens diketahui memiliki aktivitas antijamur. Pada penelitian ini, isolat bakteri Bacillus amyloliquefaciens strain N1 yang berhasil diisolasi dari tembolok ayam oleh laboratorium Program Studi Biologi Universitas Pelita Harapan diteliti kemampuan aktivitas antijamurnya. Dua spesies jamur, Aspergillus welwitschiae dan Penicillium sp. dipergunakan sebagai target dalam percobaan dengan metode whole-cell co-culture. Hasil percobaan menunjukkan bahwa Bacillus amyloliquefaciens strain N1 menghambat pertumbuhan Aspergillus welwitschiae dan Penicillium sp. masing-masing sebesar 73,3% dan 100%. Percobaan selanjutnya adalah analisis bioinformatika dengan RAST dan nucleotide BLAST database berhubung tersedianya data whole genome dari Bacillus amyloliquefaciens strain N1, dimana ditunjukkan kemungkinan bahwa senyawa antijamur yang dihasilkan adalah bacillomycin D, surfactin dan fengcyn. Kesimpulan kami adalah bahwa Bacillus amyloliquefaciens strain N1 memiliki potensi untuk dipergunakan lebih lanjut berkat aktivitas antijamurnya.Kata kunci: analisis bioinformatika; antijamur; Aspergillus welwitschiae; Bacillus amyloliquefaciens strain N1; Penicillium sp.
Analisis Aktivitas Antioksidan Ekstrak Daun Kedondong (Spondias dulcis) Menggunakan Response Surface Methodology [Analyzing the Antioxidant Activity of Ambarella Leaf Extract (Spondias dulcis) using Response Surface Methodology] Adhiwijaya, Ruben Patrick; Sugata, Marcelia; Jo, Juandy
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 5 No. 2 (2022): MAY
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jstfast.v6i1.5314

Abstract

Antioxidants are substances that mitigate the impacts of free radicals on the body, in which they can be extracted from plants. One common Indonesian plant that has a potential to be used as a source of antioxidants is the ambarella plant (Spondias dulcis). The aim of this research is to measure the antioxidant activity of ambarella leaf’s extract via response surface methodology to determine the optimum conditions required to facilitate maximum extraction. Ambarella leaves were dried and soaked in methanol by using various combinations of time, methanol concentration and solid:solvent ratio (SSR). Antioxidant activity was measured using the DPPH test. The Response Surface Methodology (RSM) was utilized to determine the optimum conditions. The obtained optimum conditions were 26.5 hours, 82.2% methanol and a solid:solvent ratio of 1:14.6. Bahasa Indonesia Abstract:Antioksidan adalah sejenis senyawa yang dapat meminimalkan pengaruh radikal bebas dalam tubuh, dimana senyawa tersebut dapat diekstraksi dari tanaman. Salah satu tanaman di Indonesia yang memiliki potensi digunakan sebagai sumber antioksidan adalah kedondong (Spondias dulcis). Tujuan penelitian ini adalah untuk mengukur aktivitas antioksidan ekstrak daun kedondong, serta menemukan kondisi yang optimum untuk ekstraksi daun kedondong dengan menggunakan response surface methodology. Daun kedondong dikeringkan lalu dimaserasi menggunakan berbagai kombinasi parameter waktu, konsentrasi pelarut metanol, dan solid:solvent ratio (SSR). Aktivitas antioksidan ekstrak lalu diukur menggunakan uji DPPH. Response Surface Methodology (RSM) digunakan untuk menemukan kondisi yang optimum. Kondisi maserasi yang optimum adalah 26,5 jam, konsentrasi pelarut metanol 82,2% dan SSR 1:14,6.
AKTIVITAS ANTIMIKROBIAL Lactiplantibacillus plantarum F75 DAN SU-KC1a TERHADAP PERTUMBUHAN BAKTERI PATOGEN [ANTIMICROBIAL ACTIVITIES OF Lactiplantibacillus plantarum F75 AND SU-KC1a AGAINST THE GROWTH OF PATHOGENIC BACTERIA] Widjaja, Anasthasia Nathania; Sugata, Marcelia; Jo, Juandy
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 6 No. 1 (2022): NOVEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jstfast.v6i2.6085

Abstract

Lactic acid bacteria (LAB), including Lactiplantibacillus species, are important within the food industry, as antimicrobial-exhibiting LAB are utilized as a bioprotective culture to increase food quality and safety. However, antimicrobial substances and other beneficial properties should be considered strain-specific, hence further characterization at the strain level is needed. Lactiplantibacillus plantarum SU-KC1a and F75 have been isolated by the Biology Study Program, Universitas Pelita Harapan. In this study, the antimicrobial activity of L. plantarum SU-KC1a and F75 against Escherichia coli and Staphylococcus aureus was evaluated. The results showed that supernatants of L. plantarum SU-KC1a and F75 could inhibit the growth of E. coli and S. aureus. Subsequent experiments suggested that the antimicrobial activity of both strains of L. plantarum were likely due to the production of organic acids that eventually decreased the pH.Bahasa Indonesia Abstract:Bakteri asam laktat (BAL), termasuk Lactiplantibacillus species, memiliki peran yang penting dalam industri makanan, karena BAL yang menunjukkan aktivitas antimikroba dipergunakan sebagai kultur bioprotektif untuk meningkatkan kualitas dan keamanan pangan. Namun, senyawa antimikroba dan juga sifat menguntungkan lainnya seharusnya dianggap lebih spesifik terhadap masing-masing strain, sehingga karakterisasi lebih lanjut pada tingkat strain perlu dilakukan. Lactiplantibacillus plantarum SU-KC1a dan F75 telah diisolasi oleh Program Studi Biologi Universitas Pelita Harapan. Pada penelitian ini, aktivitas dilakukan evaluasi antimikroba dari L. plantarum SU-KC1a dan F75 terhadap Escherichia coli dan Staphylococcus aureus. Hasil dari penelitian ini menunjukkan bahwa supernatan dari L. plantarum SU-KC1a dan F75 memiliki aktivitas antimikroba terhadap E. coli dan S. aureus. Hasil uji lebih lanjut mengindikasikan bahwa aktivitas antimikroba dari kedua strain L. plantarum lebih dikarenakan oleh produksi asam organik yang menurunkan pH.
Co-Authors Abigail Christy, Suawa Natania Adhiwijaya, Ruben Patrick Advelina Hubertha Fanggidae Anasthasia Nathania Widjaja Andareas, Pangeran Ari Prayitno, Ari Ariela Samantha Aruan, Maroloan Badriul Hegar Basrowi, Ray Wagiu Budi Setiabudiawan Celeste Abigail Thanos Chelsea Valeria Simamora Christy, Suawa Natania Abigail Daniel Elia Firmansyah Dela Rosa Dilantika, Charisma Ernestine Arianditha Pranasti Febriani, Enjelia Feronia Reni Cyrena Santoso Firmansyah, Daniel Elia Friska Dewi Sari Hutauruk Harahap, Alida Roswita Irawan, Michella Ivo Novita Sah Bandar Jevon Lesmana Kandou, Abigail Sabrina Kartjito, Melissa Stephanie Kristin Marbun Kristin Talia Marbun Lesmana, Jevon Aaron Litanto, Vivian Marbun, Kristin Talia Margaretha Lodwinia Maroloan Aruan Octaviana, Cindy Pangeran Andareas Pongajow, Billy Yosua Costantin Putri, Angela Clarita Putri, Nathania Calista Reinhard Pinontoan Retno Asti Werdhani Ruben Patrick Adhiwijaya Samantha, Ariela Sanjaya, Astia Sara Thalia Subroto Satya Nugroho Satya Nugroho Simamora, Chelsea Valeria Sitorus, Nova Soentoro, Samuel E Soewono, Effendi Sophiano, Theophilus Soputro, Veronica Tammy Sri Rezeki S. Hadinegoro, Sri Rezeki S. Suawa Natania Abigail Christy Subroto, Sara Thalia Sugata, Marcelia Surapsari, Juwalita Suryadi, Jennifer Claudia Tan Tjie Jan, Tan Tjie Thanos, Celeste Abigail Thenoch, Adrian Samuel Theodora, Gisella Theresia Santi, Theresia Timothy, Michael Tjhajadi, Jennifer Patricia Victor, Hans Vidian, Valerie Vivian Litanto Watumbara, Medista Lisa Widjaja, Anasthasia Nathania Wijaya, Accoladea Yap, Cecilia Aileen Zakiudin Munasir