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All Journal Jurnal Ilmu Kimia Universitas Brawijaya Research Journal of Life Science Natural B The Journal of Pure and Applied Chemistry Research Jurnal Ternak Tropika Jurnal Teknologi Laboratorium Jurnal Riset Agribisnis dan Peternakan JKPK (Jurnal Kimia dan Pendidikan Kimia) JSMARTech : Journal of Smart Bioprospecting and Technology Makara Journal of Science
Anna Roosdiana
Chemistry Department, Brawijaya University Jalan Veteran, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Veterinary Other

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Determination of Immobilization Optimum Conditions of Trichoderma viride’s Xilanase on Acid-Activated Zeolite Matrix Sutrisno, Sutrisno; Roosdiana, Anna; Prasetyawan, Sasangka; Sari, Intan Permata
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 2, No 2 (2017): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.801 KB) | DOI: 10.20961/jkpk.v2i2.11912

Abstract

Xylanase is an enzyme that catalyzes the reaction of xylan hydrolysis into xylose. The free enzyme can be used only once, therefore it needs to be made in the form of immobilization. This study aims to determine the optimum conditions for immobilization of xylanase using an acid-activated zeolite. The xylanase immobilization was performed on variations of shaking time (1-5) hours, variation of xylanase concentration (2 - 4 mg / mL) using 0.1 g zeolite at room temperature and a shaking rate of 100 rpm. The amount of xylanase adsorbed on the zeolite was determined by spectrophotometry using the Biuret reagent and the immobilized xylanase activity formed was determined spectrophotometrically using a DNS reagent The results showed that the optimum condition of xylanase immobilization at zeolite was achieved at 3 hrs shaking time and xylanase concentration 3.5 mg / mL with xylanase adsorbed of 156.5 mg/g zeolite and activity 26.67 units.
MOLECULAR DOCKING APPROACH OF POTENTIAL ALPHA-GLUCOSIDASE INHIBITORS FROM EXTRACTS COMPOUNDS OF R. TUBEROSA L Safitri, Anna; Tirto Sari, Dewi Ratih; Roosdiana, Anna; Fatchiyah, Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol 1, No 2 (2020)
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.017 KB) | DOI: 10.21776/ub.jsmartech.2020.001.02.1

Abstract

The present study investigates anti-diabetic capacity of compounds enclosed in the R. tuberosa L. root extracts by molecular simulation approach to examine the potential of those compounds acting as alpha-glucosidase inhibitors. Compounds chosen were cirsimarin, cirsimaritin, and sorbifolin; quercetin was used for the reference. Those compounds were downloaded from PubChem database, and human alpha-glucosidase 3D structure was obtained from Protein Data Bank. The protein was docked to the flavonoid compounds using HEX 8.0 software and visualized using Discovery Studio 4.1. The interactions of cirsimarin, cirsimaritin, sorbifolin, and quercetin on alpha-glucosidase showed similar binding patterns. They interacted with the active sites of the enzyme, causing inhibition on enzyme activity. The interactions between proteins and ligands were mostly through formation of hydrogen bonds and Van der Waals forces. The binding energy of cirsimarin cirsimaritin, sorbifolin, and quercetin to alpha glucosidase were comparable at -323.3, -279.4, -256.8, and -241.5 cal/mol, respectively. These confirm that compounds contained in the extracts of R. tuberosa L have capacity to be used as inhibitor for alpha glucosidase. 
The potency of green grass jelly extract (Premna oblongifolia Merr) as antihyperlipidemia towards aorta histopathology representation of rat (Rattus norvegicus) induced with high fatty diet (HFD) Winthoko, Eka Nora Vitaloka Aprilia Putri; Roosdiana, Anna; Pratama, Dyah Ayu Oktaviani A; Nugraha, Jusak; Purwanta, Marijam; Rifa’I, Muh Husni; Rendy, Achmad Nur
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.174

Abstract

Green grass jelly (Premna oblongifolia merr) is a plant containing fiber and chlorophyll which can lower cholesterol and triglyceride levels. This study have aims to investigate the potency of green grass jelly extract (Premna oblongifolia Merr) to prevent hyperlipidemia. The animal mode used for this study is male Rattus norvegicus, Wistar strain, the age of 8 weeks, and weight of 200 g which is divided into 5 groups of treatment namely group Kn (negative control), Kp (positive control), Kp1, Kp2, and Kp3 induced with HFD and green grass jelly extract at a dose of 5.27 g/ kg BW/ daily, 8.43 g/ kg BW/ daily, 9.37 g/ kg BW/ daily. The data of infiltrating fatty cells and macrophage on aorta histopathology was analyzed by description. This research showed that the treatment of green grass jelly extract (Premna oblongifolia Merr) to animals of hyperlipidemia model reduced infiltration fatty cells and macrophage. The conclusion of this study was the green grass jelly extract was able to prevent an increase of fatty cells and macrophage infiltration of rat (Rattus noervegicus) induced with HFD on dose 9.37 g/ kg/ BW/ daily.
Modeling of Aqueous Root Extract Compounds of Ruellia tuberosa L. for Alpha-Glucosidase Inhibition Through in Silico Study Safitri, Anna; Sari, Dewi Ratih Tirto; Fatchiyah, Fatchiyah; Roosdiana, Anna
Makara Journal of Science Vol. 25, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to analyze the inhibitory activities of aqueous root extract compounds of Ruellia tuberosa L. toward alpha-glucosidase protein by computational docking analysis. Three major compounds contained in the extracts (i.e., betaine, daidzein, and hispidulin) were selected as ligands; quercetin and acarbose were used as the reference. Computational docking analysis was performed using the HEX 8.0.0 program and visualized using the Discovery Studio Visualizer v19.1.0.18287 (2019 version) on the basis of the scoring functions. The interactions between ligands and alpha-glucosidase protein showed different binding patterns. The types of bonds involved in the interaction between the enzyme and these ligands were hydrogen and hydrophobic bonds. Energy generated from docking of betaine, daidzein, hispidulin, quercetin, and acarbose to alpha-glucosidase protein were −167.6, −249.5, −251.2, −241.5, and −322.1 cal/mol, respectively. Acarbose had the lowest energy, indicating that it has the strongest interaction with alpha-glucosidase, followed by hispidulin, daidzein, quercetin, and betaine. Amino acid residues that interacted with the ligands were His717, Met363, Arg608, Pro361, Phe362, Leu865, Glu869, Arg594, andAsp356. The current research shows that R. tuberosa L. aqueous root extracts have the potential to be used as an inhibitor for the alpha-glucosidase protein and as an antidiabetic agent. Nonetheless, further studies are needed to support this modeling study.
Incidence of Extended Spectrum Beta-Lactamase (ESBL) of Escherichia Coli Isolated from the Feces of Dairy Cattles in Blitar Dameanti, Fidi Nur Aini Eka Puji; Amri, Indah Amalia; Roosdiana, Anna; Pratama, Alfaro Rikko; Izofani, Safira; Fahmiantika, Reza; Tedja, Dhaneswara; Sutrisno, Rahayu; Akramsyah, Muhammad Ali
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.5

Abstract

Antibiotic resistance (AMR) has caused a serious global threat to human health, animal health and food security. The antibiotic resistance problem, especially in Gram-negative bacteria, is increasingly widespread in various areas and has increased mortality, morbidity, and incidence. This study aims to determine the incidence of ESBL E. coli and the AMR and MDR properties of E.coli isolated from the feces of dairy cattles in Blitar, which have not been reported. This research was conducted from June to September 2022 with 60 samples of dairy cattle feces in Blitar. Samples were isolated and identified until confirmed that 55 (91.6%) samples were E. coli. All samples showed AMR character with 100% streptomycin, 87.27% chloramphenicol, 85.45% ampicillin, 72.73% cefotaxime, 40% tetracycline, 21.82% ciprofloxacin, and 14.55% sulfamethoxazole-trimethoprim. Forty-nine samples were MDR, with 10.9% resistant in three groups, 34.5% in four groups, 29.1% in five groups, 10.9% in six groups, and 3.6% in seven groups. MDR E. coli isolates were then confirmed for ESBL using the DDST test. The incidence of ESBL-producing E. coli from dairy cattle feces in Blitar was 40% (22 isolates). The results of this study can be a warning in the human health, animal health and food security.
Evaluation of Bioactive Compounds, Release Profiles, and Toxicity Test from Microcapsules Containing R. tuberosa L. Extracts Utilizing Gum Arabic Almayda, Nabila; Roosdiana, Anna; MASRURI, Masruri; Safitri, Anna
The Journal of Pure and Applied Chemistry Research Vol. 13 No. 1 (2024): Edition January-April 2024
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2024.013.01.3336

Abstract

This study is essential to analyze the toxicity and release of bioactive compounds in R. tuberosa L. extract microcapsules coated with gum Arabic and to provide information on the use of microcapsules as a drug delivery system with controlled release of active ingredients. Investigations focused on the effect of pH and timing on discharge kinetics, as well as toxicity evaluation. The release of active ingredients from microcapsules was carried out in medium variations, namely pH 2.2 and pH 7.4, with release durations of 30, 60-, 90-, 120-, and 150-min. Bioactive compounds were released by 59.23% at pH 2.2 and 58.21% at pH 7.4 within 150 min. The BSLT (brine shrimp lethal toxicity) assay was used using brine shrimp larvae (Artemia salina L.), resulting in a non-toxic classification as evidenced by an LC50 value of 5729 μg/mL. Microcapsules in optimum conditions were then characterized using FTIR (Fourier-Transform Infrared) and SEM (scanning electron microscopy) instruments. The FTIR analysis showed variations in functional groups in R. tuberosa L. extracts, gum Arabic, and microcapsules. Furthermore, the SEM examination highlighted the morphology of microcapsules that were mostly spherical.
Immobilization of Cutinase from Fussarium oxysporum into Sea Sand Matrix for Catalytic Degradation of Polyethylene Terephthalate (PET) Prameswari, Tira P. K.; Sutrisno, Sutrisno; Roosdiana, Anna
The Journal of Pure and Applied Chemistry Research Vol. 13 No. 1 (2024): Edition January-April 2024
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2024.013.01.3325

Abstract

Cutinase is an enzyme that can be used as a catalyst in the hydrolysis reaction of polyethylene terephthalate (PET) waste into terephthalic acid and ethylene glycol. Generally, cutinase has low stability in its free form. Therefore, to increase cutinase stability, cutinase needs to be immobilized. In this study, cutinase was immobilized into a sea sand matrix by adsorption technique. The purpose of this study was to determine the optimum conditions of PET hydrolysis (pH, temperature, and incubation time), and to study the ability of immobilized cutinase for catalytic degradation of PET. The results show that immobilized cutinase has optimum conditions at pH 8, temperature of 50 °C, and incubation time of 24 hrs. Moreover, the immobilized cutinase can be used for up to three cycles with a residual activity percentage of 66.2% and an activity value of 23.224 nmol. g-1. min-1. The total enzyme activity of immobilized cutinase after being used in three cycles was 89.99 nmol. g-1. min-1 or 28.6% of its free enzyme activity.
Pengaruh Pemberian Tablet Konvensional Kasein Yogurt Susu Kambing Terhadap Akumulasi Residu Dioksin dan Kerusakan Jaringan Musculus Pectoralis Pada Ayam Broiler Yang Dipapar Dioksin Anjani, Godelva Permata; Haskito, Ajeng Erika Prihastuti; Roosdiana, Anna; Pratiwi, Herlina; Mahdi, Chanif
TERNAK TROPIKA Journal of Tropical Animal Production Vol. 23 No. 1 (2022): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2022.023.01.10

Abstract

Dioksin merupakan turunan dari Polychlorinated trichloro-dibenzo-p-dioxins (TCDDs) yang bersifat lipofilik dan bioakumulasi. Dioksin mampu berikatan dengan Aryl hydrocarbon Receptors maupun Transforming Growth Faktor Beta, sehingga sitokrom P450 teraktivasi dan memicu kerusakan jaringan. Kasein yogurt susu kambing merupakan sumber peptida bioaktif dan antioksidan. Penelitian ini bertujuan untuk melihat pengaruh tablet konvensional kasein yogurt susu kambing terhadap kadar residu dioksin dan kerusakan jaringan musuculus pectoralis ayam broiler yang terpapar dioksin. Penelitian ini menggunakan 18 ekor ayam broiler berusia 21 hari, dibagi menjadi 3 kelompok, terdiri atas: 1) Kelompok negatif (tanpa perlakukan); 2) Kelompok positif (TCDD 50 ng/kg pakan/hari); 3) Kelompok terapi (TCDD 50 ng/kg pakan/hari + tablet konvensional kasein yogurt susu kambing 750 mg/ekor peroral). Kadar residu dioksin diukur dengan metode spektrofotometri-UV diolah sebagai data kuantitatif dianalisa dengan sidik ragam (ANOVA) dilanjutkan Uji Tukey. Histopatologi musculus pectoralis diwarnai dengan menggunakan metode pewarnaan Hematoxylin-Eosin (HE) dan diamati secara deskriptif. Hasil penelitian menunjukkan analisa statistik pemberian tablet konvensional kasein yogurt susu kambing memberikan pengaruh tidak berbeda nyata (p>0.05) pada penurunan kadar residu dioksin, namun data pengukuran residu dioksin menunjukkan bahwa adanya penurunan kadar residu dioksin sebesar 18,20%. Pemberian tablet konvensional kasein yogurt susu kambing juga terbukti dapat memperbaiki kerusakan jaringan musculus pectoralis ayam broiler (Gallus domesticus) yang dipapar dioksin dengan mengurangi jumlah jaringan ikat perimisium yang mengalami penebalan. Kesimpulan penelitian ini adalah pemberian tablet konvensional kasein yogurt susu kambing tidak memberi pengaruh yang signifikan untuk menurunkan kadar residu TCDD dan dapat memperbaiki kerusakan histopatologi musculus pectoralis ayam broiler (Gallus domesticus) yang dipapar dioksin.
Co-Authors A.A. Ketut Agung Cahyawan W Adyatama Ardian Akhmad Kholisul Fuad Akramsyah, Muhammad Ali Almayda, Nabila Amri, Indah Amalia Anjani, Godelva Permata Aulanni'am, Aulanni'am Ayunda Arum Sari Chanif Mahdi Dameanti, Fidi Nur Aini Eka Puji Diah Mardiana Edi Sukmana Elvanila Tanjung Fahmiantika, Reza fatchiyah . Firmawati, Aulia Haskito, Ajeng Erika Prihastuti Herawati Herawati Iftakhul Mufarrikha Imaroh Isya intan permata sari Izofani, Safira Janatun Na’imah Jusak Nugraha Kurniasari, Balqis Larasati, Luckyta Retno Masruri Masruri Mufida, Fadillah Prameswari, Tira P. K. Pratama, Alfaro Rikko Pratama, Dyah Ayu Oktaviani A Pratama, Dyah Ayu Oktavianie Ardhiana Pratiwi, Herlina Purwanta, Marijam Putri Dewi Rendy, Achmad Nur Rifa’I, Muh Husni Rosmanansari, Novia Sintesa Dara Safitri, Anna Salamah, Alfi Sari, Dewi Ratih Tirto Sasangka Prasetyawan Satriana Satriana Selvi Marcellia Sutrisno, Rahayu Sutrisno, Sutrisno Tantowidjojo, Vanessa Rachmani Tedja, Dhaneswara Thaati, Hayyunisa Widyanti, Marta Wijaya, Anik Sri Windari, Hema Aprilia Setyo Winthoko, Eka Nora Vitaloka Aprilia Putri