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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Medical Journal of Indonesia Indonesian Journal of Human Nutrition Majalah Kesehatan FKUB Journal of Issues in Midwifery Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Keperawatan (JKEP) Asian Journal of Health Research Jurnal Kedokteran Hewan Journal of Clinical Microbiology and Infectious Diseases
Sanarto Santoso
Fakultas Kedokteran Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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EKSPRESI PROTEIN ADHF36 PADA PERUBAHAN OSMOLARITAS SERTA pH LINGKUNGAN HIDUP SALMONELLA TYPHI SECARA IN VITRO I Nengah Kundera; Sanarto Santoso; Aulanni’am a; Sri Winarsih
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i1.351

Abstract

Penelitian ini bertujuan mengamati stabilitas ekspresi protein AdhF36 Salmonella typhi pada perlakuan osmolaritas dan pH dengan metode eksperimen laboratorium-eksploratif. Hasil penelitian ini menunjukkan perbedaan ekspresi protein AdhF36 Salmonella typhi pada perlakuan osmolaritas dengan pH. Hasil SDS-PAGE menunjukkan bahwa pita protein AdhF36 tetap terekspresi pada perlakuan osmolaritas 50-350 mM, sebaliknya pita protein ini tidak terdeteksi pada perlakuan pH 4,5-6,0. Hal ini didukung oleh hasil uji Western Blot yang berhasil mendeteksi protein AdhF36 hanya pada perlakuan osmolaritas. Hasil ini membuktikan bahwa Salmonella typhi lebih toleran terhadap perubahan osmolaritas dibandingkan dengan pH.
Chemical Compounds and Antibacterial Activity of Thymus Vulgaris Leaves’ Ethanolic Extract Against Salmonella typhimurium: Thymus vulgaris Against Salmonella typhimurium Abousouh, Emad Khaleefah; Endharti, Agustina Tri; Santoso, Sanarto; Santosaningsih, Dewi
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.09

Abstract

Invasive non-typhoidal Salmonella is an important pathogen that causes life-threatening bloodstream infections. Antibiotic resistance in non-typhoidal Salmonella has emerged as a result of the increasing use of antibiotics; therefore, an alternative source of therapeutic agents is required. This study aimed to investigate the active compound and antibacterial activity of Thymus vulgaris leaves’ ethanolic extract against Salmonella typhimurium. Thin-layer chromatography was performed to identify the active compound in the ethanolic extract of T. vulgaris leaves. The antibiotic effect of the extract was carried out by agar dilution assay using the following concentrations: 0%, 2.5%, 5.0%, 7.5%, 10.0%, 12.5%, and 15.0% (w/v). Thymol and carvacrol were detected in the thin-layer chromatography. The minimum inhibitory concentration of the extract was 10.0% (w/v). In conclusion, T. vulgaris leaves’ ethanolic extract demonstrated antimicrobial activity against S. typhimurium. Further investigation is required to analyze the role of thymol and carvacrol as active compounds against S. typhimurium.
Level of SARS-CoV-2 exposure post the CoronaVac vaccination in health workers at Dr. Saiful Anwar General Hospital, Malang Bormasa, Pelpina; Santoso, Sanarto; Diyah, Nurima; Wahono, Cesarius Singgih; Retnoningsih, Dewi
Journal of Clinical Microbiology and Infectious Diseases Vol. 4 No. 1 (2024): Available online: June 2024
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v4i1.46

Abstract

Introduction. COVID-19 is considered a new emerging infection due to SARS-CoV-2. CoronaVac refers to an inactivated SARS-CoV-2 vaccine obtaining EUL (Emergency use listing) from BPOM RI and WHO. This vaccine was previously reported to exhibit good efficacy in generating protective antibodies against SARS-CoV-2. Nevertheless, health workers in Indonesia might pose a high risk of COVID-19 exposure, leading to mortality. This study aims to identify the level of SARS-CoV-2 exposure to the CoronaVac vaccination in health workers at Dr. Saiful Anwar General Hospital, Malang. Methods. This study implemented analytical observational research with a prospective cohort approach on health workers at Dr. Saiful Anwar General Hospital, Malang, who received the 2nd dose of vaccination dose with a 14-day interval. All study subjects were observed for 6 months by examining the level of exposure to SARS-CoV-2 obtained from the results of nasopharyngeal and oropharyngeal swab examinations of SARS-CoV-2 RT PCR examination. Categorical data were tested by using Chi-square or Fisher exact. Results. From 184 samples, the SARS-CoV-2 exposure indicated several outcomes: positive 57 (31 %) and negative 127 (69%). Severity degrees of COVID-19 disease were asymptomatic 17 (29.8%), mild 37 (64.9), moderate 3 (5.3%), severe 0 (0%), and there were no mortality cases. The time (duration) of exposure to SARS-CoV-2 post-CoronaVac vaccination indicated that the highest number of participants with positive RT-PCR swab results post-vaccination was in the fifth month of the CoronaVac vaccination with 41 people. Clinical symptoms of SARS-CoV-2 exposure experienced by participants post the CoronaVac vaccination included pneumonia (3 people), cough and cold (27 people), fever (22 people), myalgia (17 people), and sore throat (11 people). There was no significant relationship p value > 0.05 between gender, age, comorbidities, and Ct value on SARS-CoV-2 exposure post CoronaVac vaccination in health workers at Dr. Saiful Anwar General Hospital, Malang. Conclusion. There was SARS-CoV-2 exposure of 31% post the CoronaVac vaccination in health workers at Dr. Saiful Anwar General Hospital, Malang.
Chemical Compounds and Antibacterial Activity of Thymus Vulgaris Leaves’ Ethanolic Extract Against Salmonella typhimurium: Thymus vulgaris Against Salmonella typhimurium Abousouh, Emad Khaleefah; Endharti, Agustina Tri; Santoso, Sanarto; Santosaningsih, Dewi
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.09

Abstract

Invasive non-typhoidal Salmonella is an important pathogen that causes life-threatening bloodstream infections. Antibiotic resistance in non-typhoidal Salmonella has emerged as a result of the increasing use of antibiotics; therefore, an alternative source of therapeutic agents is required. This study aimed to investigate the active compound and antibacterial activity of Thymus vulgaris leaves’ ethanolic extract against Salmonella typhimurium. Thin-layer chromatography was performed to identify the active compound in the ethanolic extract of T. vulgaris leaves. The antibiotic effect of the extract was carried out by agar dilution assay using the following concentrations: 0%, 2.5%, 5.0%, 7.5%, 10.0%, 12.5%, and 15.0% (w/v). Thymol and carvacrol were detected in the thin-layer chromatography. The minimum inhibitory concentration of the extract was 10.0% (w/v). In conclusion, T. vulgaris leaves’ ethanolic extract demonstrated antimicrobial activity against S. typhimurium. Further investigation is required to analyze the role of thymol and carvacrol as active compounds against S. typhimurium.
Co-Authors A.A. Ketut Agung Cahyawan W Abousouh, Emad Khaleefah Agustina Tri Endharti Alvi Milliana Anggun Rindang Cempaka Aulanni'am, Aulanni'am Aulia Abdul Hamid Avin Ainur Fitrianingsih Bambang Rahardjo Bormasa, Pelpina Dewi Santosaningsih Dian Chusna Mufida Diyah, Nurima Erikawati, Dewi Gani, Agus A Hanifarizani, Rahma Dian I Nengah Kundera I Wayan Agung Indrawan Issiyah, Nurani Lailia Nur Rachma Laksmi Karunia Tanuwijaya M. Fendi Nur Yahya Maimun Z Arthamin, Maimun Z Mutaqin, Fadilah Ni Luh Putu HM Nur Sophia Matin Nurdiana Nurdiana Nurma Afiani Nurtjahjo Budi Santoso Nyoman Artha Megayasa, Nyoman Artha Prawiro, Sumarno Reto Regina Ayu Fristiyanti Retnoningsih, Dewi Roekistiningsih Roekistiningsih Safrina Dewi Ratnaningrum Sjoekoer M. Dzen Sri Winarsih Sumakto Sumakto Sumarno Sumarno Tina Handayani N Tinny Endang Hernowati Wahono, Cesarius Singgih Wisnu Barlianto