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All Journal JURNAL SAINS PERTANIAN EQUATOR Jurnal Teknologi Pertanian Andalas AgriHumanis: Journal of Agriculture and Human Resource Development Studies Unri Conference Series: Community Engagement Sagu Publikasi Riset Mahasiswa Manajemen Teknologi dan Industri Pertanian Indonesia Al-Zayn: Jurnal Ilmu Sosial & Hukum Causa: Jurnal Hukum dan Kewarganegaraan JURNAL MULTIDISIPLIN ILMU AKADEMIK Jurnal Intelek Insan Cendikia Journal of Industrial and Mechanical Engineering Jurnal BOLA : Bersama Olahraga Laju Asia
Edo Saputra
Jurusan Teknologi Pertanian, Fakultas Pertanian, Universitas Riau
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VARIASI PENGGUNAAN SUKROSA PADA MEDIUM NATA DE BANANA SKIN TERHADAP KINETIKA PERTUMBUHAN SELULOSA MIKROBIAL Alexander Hutagaol; Fajar Restuhadi; Emma Riftyan; Edo Saputra
Jurnal Sagu Vol 22, No 1 (2023)
Publisher : Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/sagu.22.1.p.8-14

Abstract

Nata de banana skin  merupakan nata yang berasal dari kulit pisang. Pertumbuhan mikroba berguna secara kuantitatif dengan studi kinetika yang disetel untuk mengetahui pertumbuhan selulosa dengan kecepatan maksimum (V  maks  ) dan pengaturan  Michaelis-Menten  (K  M  ). Penelitian ini bertujuan untuk menentukan formula sukrosa terbaik dan kecepatan maksimum (V  max  ) serta konstanta  Michaelis-Menten  (K  M ) pada selulosa mikroba yang dihasilkan. Penelitian ini dilakukan secara eksperimental dengan menggunakan rancangan acak lengkap dengan empat perlakuan dan empat ulangan sehingga diperoleh 16 satuan percobaan.Perlakuan dalam penelitian ini adalah P1 (5% sukrosa), P2 (7% sukrosa), P3 (9% sukrosa) dan P4 (11% sukrosa). Data yang diperoleh dianalisis dengan menggunakan  analysis of variance (ANAVA). Jika Fhitung ≥ Ftabel maka dilanjutkan dengan Uji Jarak Berganda Duncan (DMRT) pada taraf 5%. Hasil variansi menunjukkan bahwa kintika pertumbuhan biomassa selulosa mikroba pada pembuatan nata de banana skin dengan penggunaan sukrosa berpengaruh nyata terhadap ketebalan nata, bobot basah nata, bobot kering nata. rendemen dan kadar gula total. Formulasi perlakuan penambahan sukrosa terbaik pada penelitian ini adalah perlakuan P3 (sukrosa 9%) yang memiliki ketebalan nata 0,30 cm, berat basah nata 6,18 g, berat kering nata 0,079 g, rendemen 5,16%.Nilai kinetika pertumbuhan biomassa mikrobial selulosa kulit pisang Nata de yang dihasilkan adalah kecepatan maksimum (V  max  ) sebesar 0,96019 μM/ml dan konstanta optimum menurut  Michaelis-Menten  (KM  ) 0,61614 μM.
VARIASI PENGGUNAAN SUKROSA PADA MEDIUM NATA DE BANANA SKIN TERHADAP KINETIKA PERTUMBUHAN SELULOSA MIKROBIAL Hutagaol, Alexander; Restuhadi, Fajar; Riftyan, Emma; Saputra, Edo
SAGU Vol. 22 No. 1 (2023): SAGU Journal – Agri. Sci. Tech., Maret, 2023, Vol. 22 : No. 1
Publisher : Fakultas Pertanian Universitas Riau

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Abstract

This study aims to determine the best sucrose formulation and the maximum velocity (Vmax) and Michaelis-Menten constant (KM) on the resulting microbial cellulose. This research was conducted experimentally using a completely randomized design with four treatments and four replications to obtain 16 experimental units. The treatments in this study were P1 (5% sucrose), P2 (7% sucrose), P3 (9% sucrose), and P4 (11% sucrose). The data obtained were analyzed using analysis of variance (ANOVA). If Fcount ≥ Ftable, it will be continued with the Duncan’s multiple range test (DMRT) at the 5% level. The results of variance showed that the growth kinetics of microbial cellulose biomass in the manufacture of nata de banana skin with the use of sucrose had a significant effect on the thickness of the nata, the wet weight of the nata, the dry weight of the nata, the yield and the total sugar content. The best formulation treatment for adding sucrose in this study was treatment P3 (9% sucrose), which had a thickness of 0.30 cm nata, wet weight of nata 6.18 g, dry weight of nata 0.079 g, yield 5.16%. The growth kinetics value of Nata de banana skin microbial cellulose biomass produced was the maximum speed (Vmax) of 0.96019 μM/ml and constant optimum according to Michaelis-Menten (KM) 0.61614 μM.
Co-Authors - Andasuryani Abimanyu Abimanyu Aji Wibowo Alexander Hutagaol Alfarel Kurniawan Anthony Hamzah Ayu Efritadewi Candra, Jumi Dewi Fortuna Ayu Dewi, Yossie Kharisma Dimas Prasetya Edfan Darlis Fajar Restuhadi Heni Widiyani Hutagaol, Alexander Joni Gunawan Keflin, David Kevin Oksandy Ramadhani Masrul Muhammad Idhamsyah Natalia Natalia Nathanael Pratama Rezky Nelson Adi Riyanto Nopiani, Yanti Nurmalina Nurmalina, Nurmalina Paundra, Fajar Pramudya, Daka putra, Ahmad Saputra Putri Ayuni Raswen Efendy Riduansyah Riduansyah Riftyan, Emma Saat Egra Santosa Santosa Santoso, Dwi Saputra, Andhyka Jaya Satrio Aji Pratama Shanti Fiftriani Shanti Fitriani Sirait, Sudirman Sudirman Sirait Syafitri Haryani Tri Suci, Yunisa USMAN PATO Vonny Setiaries Johan Widagdo, Herry Yunisa Tri Suci Yusmarini Yusmarini