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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Pasir Laut Management of Aquatic Resources Journal (Maquares) AL KAUNIYAH Jurnal Harpodon Borneo Maspari Journal ECSOFiM (Economic and Social of Fisheries and Marine Journal) Journal of Tropical Biodiversity and Biotechnology JFMR (Journal of Fisheries and Marine Research) Acta Aquatica: Aquatic Sciences Journal Jurnal Laut Khatulistiwa Jurnal Akuatiklestari Jurnal Penelitian Perikanan Indonesia Bawal : Widya Riset Perikanan Tangkap Jurnal Segara Journal of Aquaculture and Fish Health Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Jurnal Laut Pulau Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Suradi Wijaya Saputra
Progam Studi Manajemen Sumberdaya Perairan, Departemen Sumberdaya Akuatik Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Physics Social Sciences Other

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Journal : AL KAUNIYAH

 1 
Identification and Phylogenetic Analysis of Blue Swimming Crab (Portunus sp.) in The Northern Waters of Jepara Used DNA Barcoding Lazuardhi, Risqi; Sabdaningsih, Aninditia; Ayuningrum, Diah; Saputra, Suradi Wijaya; Hartoko, Agus; Haeruddin, Haeruddin
Al-Kauniyah: Jurnal Biologi Vol 18, No 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.38705

Abstract

The Awur Bay and Kartini Beach in Jepara, Central Java, are fishing areas with blue swimming crab as a main catch. This study aims to examine the species of blue swimming crabs captured in the Northern Waters of Jepara from different locations, analyze the molecular characteristics based on gender differences, obtain GenBank accession numbers as genetic conservation, and construct their phylogenetic tree. The research used a quantitative descriptive method. Samples were collected using purposive sampling from fishermen's catches and selected based on the difference in crab claws and carapace color, with a total of 4 samples. The samples were extracted using a DNA extraction kit and tested using Polymerase Chain Reaction (PCR) to visualize mitochondrial DNA (COI gene) and processed using MEGA 11. BLAST results showed that the similarity of the samples to Portunus pelagicus ranged from 97.02% to 99.42%. The different colors of claws and carapace did not affect the DNA sequence composition, hence indicating sexual dimorphisms. The DNA sequences from this study were deposited to GenBank with accession number LC836055.1-LC836058.1. Based on the phylogenetic tree construction, the clade of all samples shows a close relationship with the species Portunus pelagicus.
Identification and Phylogenetic Analysis of Blue Swimming Crab (Portunus sp.) in The Northern Waters of Jepara Used DNA Barcoding Lazuardhi, Risqi; Sabdaningsih, Aninditia; Ayuningrum, Diah; Saputra, Suradi Wijaya; Hartoko, Agus; Haeruddin, Haeruddin
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.38705

Abstract

The Awur Bay and Kartini Beach in Jepara, Central Java, are fishing areas with blue swimming crab as a main catch. This study aims to examine the species of blue swimming crabs captured in the Northern Waters of Jepara from different locations, analyze the molecular characteristics based on gender differences, obtain GenBank accession numbers as genetic conservation, and construct their phylogenetic tree. The research used a quantitative descriptive method. Samples were collected using purposive sampling from fishermen's catches and selected based on the difference in crab claws and carapace color, with a total of 4 samples. The samples were extracted using a DNA extraction kit and tested using Polymerase Chain Reaction (PCR) to visualize mitochondrial DNA (COI gene) and processed using MEGA 11. BLAST results showed that the similarity of the samples to Portunus pelagicus ranged from 97.02% to 99.42%. The different colors of claws and carapace did not affect the DNA sequence composition, hence indicating sexual dimorphisms. The DNA sequences from this study were deposited to GenBank with accession number LC836055.1-LC836058.1. Based on the phylogenetic tree construction, the clade of all samples shows a close relationship with the species Portunus pelagicus.
Co-Authors - Djuwito A'in, Churun Abdul Ghofar Abdul Ghofar Abdul Ghofar Abdul Kohar Mudzakir Abdul Qadir Jailani, Abdul Qadir Abdullah Habibi Adriyani, Alvita Afina Nursa Dewi, Afina Nursa Agus Hartoko Agustiari, Arinta Maulidina Ahnan, Muhammad Firhan Maftuh aji, muhammad tri aliffyana, firanika Angga Yan Prayudha Anhar Solichin Anhar Solihin Aninditia Sabdaningsih Anthonius Hot Arios Apriyanti, Anisa Dwi Arif Widiyanto Asriningpuri, Degrita Herdianti Astuti, Menur Puji Ayu Dwi Purnamawati, Ayu Dwi Ayu Okta Widjayana, Ayu Okta Ayu Rutiyaningsih Ayuningtyas Indrawati Azizi, Nurul Amin Azzahra, Rizqina Nafa Nur Bambang Sulardiono Bayu Surarso Boedi Hendrarto Bosma, Roel H. Bram Setyadji, Bram Budi Nugraha, Budi Churun Ain Damar Nusawicaksono Kurniawan, Damar Nusawicaksono Diah Ayuningrum, Diah Dian Wijayanto Djuwito Djuwito EDY H.P. MELMAMBESSY Eki Septiana Erika Kurniawati, Erika Faik Kurohman Fajrur Rohman, Fajrur Febyansyah Nur Abdullah, Febyansyah Nur Fitria Sari Fofied, Fernanda Gitarini Frida Purwanti Gabriela Ari Sulistyawati Galuh Kirana Anindhita Griselda, Adinda Putri Khairunnisa Haeruddin Haeruddin Hanggoro, Adnan Lintang Harmasnida Rachma, Harmasnida Herna Octivia Damayanti Hilda Fadhila, Hilda Huda, Anisa Arifatul Ibrahim, Putri Sapira Iin Ika Wahyuni Imam Triarso Imanuel Villian Trayanta Soukotta Iswanto, M. Fajar Fajar Johannes Hutabarat Julianto Subekti Karohmatullah, Achmad Mufidh Kartika Widya Iswara Krisliyana Mia Anggarini, Krisliyana Mia Kukuh Prakoso, Kukuh Kurniawan, Wanwan Lazuardhi, Risqi Lestari Lakhsmi Widowati Mahardhika, Septya Mega Manullang, Panogu Marwulandari, Riyani Monica, Soraya Chandra Muhamad Sutri Wardani Nisya, Choirun Norma Afiati Novi Andriani, Novi Nugraha, Satria Wiratama Nurul Mukhlish Bakhtiar Panjaitan, Theresia Pramesti Budi Widyaningrum Pratik Primas Akbar Pratiwi Genesi Siagian, Pratiwi Genesi Prijadi Soedarsono Pujiono Wahyu Purnomo Putra, Muhammad Ardinan Dwi Putri, Lintang Kinanti Ramadhan, Mochammad Rizqy Restiana Wisnu Ariyati Rian Kisworo Rini, Annisa Sulistya Ririn Vianita Rizky Oktarina Nur Dewanti Rosa Anggraeni, Rosa Sakina, Kurnia Sandro, Yosua Sari, Katrina Dwika Sari, Verina Sarjito - Schrama, Johan Shiffa Febyarandika Shalichaty Siahaan, Debora Ernika Siraj, Ahmad Zhafran Siti Aisyah Siti Rudiyanti Siti Yuliani Rochmatin Skar Puji Astuti, Skar Puji Sri Lestari Sri Rejeki Subijanto Subijanto Suryanti Sutrisno Anggoro Taufani, Wiwiet Teguh Trienes, Yoni Wahyu Kurniawan Wahyu Rizkiyana Wiedha Maharmingnastiti, Wiedha Winda Ari Widyaningtiwi Wiwiet Teguh Taufani Yalindua, Fione Yukita Yulianti, Aida Tri Zuleca, Maulidina Ziva