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All Journal Forum Pasca Sarjana Vegetalika Jurnal AgroBiogen Populis : Jurnal Sosial dan Humaniora Sains Natural: Journal of Biology and Chemistry J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Journal of Social Political Sciences (JSPS) Jurnal Abdimas Bina Bangsa Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Journal of Tropical Biodiversity Jurnal Pengabdian Masyarakat Waradin DEDIKASI : Jurnal Pengabdian Kepada Masyarakat Jurnal Umum Pengabdian Masyarakat (JUPEMAS)
Nonon Saribanon
Universitas Nasional
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Nursing Public Health Social Sciences Other

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Journal : Jurnal AgroBiogen

 1 
Molecular Analyses and Resistance Evaluation to Bacterial Leaf Blight on Swampy Rice Genotypes Ahmad Dadang; Siti Yuriah; Nonon Saribanon; Joko Prasetiyono; Dwinita Wikan Utami
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p95-104

Abstract

Swampland is a prospective marginal land to be developed for rice cultivation. Rice plants that are adaptive to swampland usually have good tolerance to Fe. Previously, we obtained several rice lines tolerant to Fe toxicity in the field. This study aimed to analyze the swampy rice genotypes using STS molecular markers linked to bacterial leaf blight (BLB)-resistant genes and evaluate the resistance level of the genotypes against three BLB races under the greenhouse condition. This research was conducted in the Molecular Biology Laboratory and Greenhouse of the Indonesian Center for Agricultural Biotechnology andGenetic Resources Research and Development from January to May 2021. Eighteen swampy rice varieties/lines and two check varieties for Fe toxicity and BLB, respectively, were used in this research. The eight STS molecular markers linked to BLB resistance genes were used for genotyping analysis. The results showed that all rice plants tested contained xa5 allele gene, 19 genotypes had Xa4 allele gene, 17 genotypes had Xa7 allele gene, 9 genotypes had xa13 allele gene, 5 genotypes had xa13 allele gene, and 6 genotypes had Xa4 and Xa21 allele genes. Line B146E-4 was estimated to have 7 resistance alleles genes, meanwhile lines B14301E-KA-17-a, B14315E-KA-1, and B13-1-a have 6 resistance allele genes. The resistance evaluation of the genotypes against the three BLB races (races III, IV, and VIII) under the greenhouse condition showed that line B14315E-KA-1 was confirmed to be highly resistant to BLB disease. This line can be a promising candidate for swampy rice that is resistant toBLB disease.
Co-Authors Aditya Kusuma, Sony Afifah Intan Kamila Ahmad Dadang Alvira Noer Effendi Alvira Noer Effendi Amarullah Amarullah, Amarullah Andri Akbar Apriani, Hesty Arbiani, Aulia Aria, Angga Arief Kurniawan Astri Oktavina Astri Oktavina Astri Oktavina Aziz, Panji B. Surata Azizi, Alfia Annur Aini Basuki Wicaksono Batari Wahyu Pangesti Batari Wahyu Pangesti Bhakti Nur Avianto Dwinita Wikan Utami E. Gumbira Sa’id Effendi, Alvira Noer Endriatmo Soetarto Erdiyanto, Angga Septian Evi Nuraeni Fauzan Cholifatullah Fauzan Cholifatullah Fauziah Ilmi Fauziah Ilmi Fauziah Ilmi Fitriah Basalamah Gustiani, Widya Hana Kifayah Hariyanto Hariyanto Hukom, Frans Alexander A. Ichsan Wahyudin Ilmi , Fauziah Ilmi, Fauziah Isfan Fajar Sukarno Joko Prasetiyono Joshi, Luthfi Kurniawan Kamelia Putri Utami Krisna, Arindya A’isy Lindayu, Feriska M Ainul Labib Melati, Latifa Sukma Muhammad Firdaus Rafzansani Njo Fransiscus Xaverius Anditya Ciptadi Putra Noverita Noverita Noverita Pangesti, Batari Wahyu Panji B Surata Azis Panji B. Surata Aziz Prabandari, Hanna Prihandini, Viola Ayu Putra, Njo Fransiscus Xaverius Anditya Ciptadi Putra, Njo Fransiscus XaveriusAnditya Ciptadi Putrawardana, Ulika Trijoga Qomariyah, Siti Fatimah Nurul R. Dhevan Cahyo Adi Putra Perdana Rafsanzani , Muhammad Firdaus Rafsanzani, Muhammad Firdaus Rahayu Lestari Rahman, Nur Hamidar Ramadhan, Muhammad Ishlah Ramadhona, Yudha Ramadlan, Ahmad Ramdani, M. Yuda Reflinur Reflinur Rubyawan , Rubyawan Saipul, Saipul Saptowo Jumali Pardal Sara Pebrina Sara Pebrina Silvia Hasan Siregar, Muhammad Abrar Putra Siregar, Zuhriansyah Siti Yuriah Slamet Slamet Sri Endarti Rahayu Sri Hartati Subagyo, Banu Sumardjo Surjono H. Sutjahjo Tatang Mitra Setia Tiaraningrum Wahyu Andrias Wening Enggarini Yenisbar Zuhriansyah Zuhriansyah, Zuhriansyah