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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Majalah Kedokteran Bandung Althea Medical Journal The Journal of Pure and Applied Chemistry Research Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Sari Pediatri Paediatrica Indonesiana Journal of Maternal and Child Health The International Journal of Tropical Veterinary and Biomedical Research Unnes Journal of Public Health
Budi Setiabudiawan
Departemen Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Dr. Hasan Sadikin Bandung
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Journal : Althea Medical Journal

 1 
Pregnant Human Myometrial 1-41 Cell Viability Test on Vitamin D Administration Aziz, Muhammad Alamsyah; Krisnadi, Sofie Rifayani; Handono, Budi; Setiabudiawan, Budi
Althea Medical Journal Vol 10, No 3 (2023)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v10n3.2750

Abstract

Background: Preterm labor is one of the universal causes of perinatal mortality worldwide. One of the causes of preterm labor is uterine muscle integrity problems. Some mechanistic studies show insight into vitamin D activity’s possible role in the injured muscle. This study aimed to determine whether vitamin D can increase muscle cell viability.Methods: This experimental research used human smooth muscle uterine myometrium cell line pregnant human myometrial (PHM) 1-41. The cells were cultured for 24 hours in hypoxia condition, then incubated with several doses of vitamin D. The PHM1-41 cell viability was measured using spectrophotometry. Data analysis was conducted using IBM SPSS 24.0. A p-value <0.05 was considered statistically significant. Results: The result showed that the minimum level of muscle cell viability after vitamin D incubation was with 300 nM administration, and the maximum level was after 10nM (88.57%+4.48 and 96.21%+2.13 respectively).Conclusions: Vitamin D at a specific dose can improve cell availability. The optimal dose to improve cell viability is 10 nM. 
Co-Authors Abdurachman Sukadi Abdurachman Sukadi Ahmad Zaeni Syafii Alex Chairulfatah Ambrosius Purba Anang Endaryanto Andhika T. Hutapea Andri Firdaus Ani Melani Maskoen Arini Setiawati Asep Aziz Asopari Asri Arum Sari Atu Purnama Dewi Ayu Alia Aziz, Muhammad Alamsyah Bakhtiar Bakhtiar Basrowi, Ray Wagiu Benny Hasan Purwara Budi Handono Cissy B. Kartasasmita Cissy B. Kartasasmita Citra Amelinda Dedi Rachmadi Diana Rosifah Diet Sadiah Rustama Dikdik Kurnia Dilantika, Charisma Dina Muktiarti, Dina Dita Lasendra Djatnika Setiabudi Dwi Prasetyo Edhyana Sahiratmadja Edhyana Sahiratmadja Elza Noviani Endang Sutedja Erny Rachmawati Triwardhani Fedri Ruluwedrata Rinawan Gaga Irawan Nugraha Gartika Sapartini Harold Eka Atmaja Harry Raspati Hendra Dian Adhita Henni Djuhaeni Herry Garna Hesti Puspasari Ida Parwati Ika Agus Rini Irma Ruslina Defi Irman Permana Irna Sufiawati Isabella Riandani Johanes C. Mose Johanes C. Mose Johannes Cornelius Mose Johannes Hudyono Juandy Jo Jusuf Sulaeman Effendi Kartjito, Melissa Stephanie Keri Lestari Ketut Dewi Kumara Wati Kuswandewi Mutyara Laniyati Hamijoyo, Laniyati Lelani Reniarti M. Thaufiq S. Boesoirie, M. Thaufiq S. Mahesa Suryanagara Melati Sudiro Melva Louisa Mieke H. Satari Minerva Riani Kadir Mohamad Yanuar Anggara Myrna Soepriadi Nur Melani Sari Perdana, Nanan Surya Putria Rayani Putria Rayani Apandi Raden Tina Dewi Judistiani Rahmat Gunadi Reni Ghrahani Reni Ghrahani Reni Ghrahani Reni Ghrahani Reni Ghrahani DM Riana Novy RINI SUNDARI RINI SUNDARI, RINI Rovina Ruslami, Rovina Saputri, R. Ayu Hardianti Sasfia Candrianita Sefty Mariany Samosir Setyorini Irianti Sheba, Shiane Hanako Sinta Boesoerie Sitorus, Nova Sjarif Hidayat Effendi Sofie R. Krisnadi Sumadiono Sumadiono Sunjaya, Deni K. Surapsari, Juwalita Susy P. Wihadi Sylvia Rachmayati Teti Madiadipoera Tetty Yuniati Tetty Yuniati Thaufiq S. Boesoirie Tisnasari Hafsah Triana Nurul Meirina Triatmaja, Dendy Vitriana Vitriana Vycke Yunivita Kusumah Dewi Weninggalih, Endah Yulia Yulia Zakiudin Munasir Zulfan M. Alibasyah